An optimized exosome production strategy for enhanced yield while without sacrificing cargo loading efficiency

Rongxin Zhang, Te Bu, Ruidan Cao, Zhelong Li, Chen Wang, Bing Huang, Mengying Wei, Lijun Yuan & Guodong Yang
Abstract Background Exosome mediated mRNA delivery is a promising strategy for the treatment of multiple diseases. However, the low yield of exosomes is a bottleneck for clinical translation. In this study, we boosted exosome production via simultaneously reducing the expression of genes inhibiting exosome biogenesis and supplementing the culture medium with red cell membrane components. Results Among the candidate genes, knocking down of Rab4 was identified to have the highest efficacy in promoting exosome biogenesis...
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