Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

Aura Maria Moreno-Echeverri, Eva Susnik, Dimitri Vanhecke, Patricia Taladriz-Blanco, Sandor Balog, Alke Petri-Fink & Barbara Rothen-Rutishauser
Abstract Background In the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental outcome. We address some of these pitfalls and suggest possible solutions. The accuracy of fluorescence microscopy methods has an important role in obtaining insights into NP interactions with lysosomes at...
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