Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material

Gordon Wiegleb, Susanne Reinhardt, Andreas Dahl & Nico Posnien
Abstract Background Recent technological advances opened the opportunity to simultaneously study gene expression for thousands of individual cells on a genome-wide scale. The experimental accessibility of such single-cell RNA sequencing (scRNAseq) approaches allowed gaining insights into the cell type composition of heterogeneous tissue samples of animal model systems and emerging models alike. A major prerequisite for a successful application of the method is the dissociation of complex tissues into individual cells, which often requires large...
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