Lensless holographic microscope with a time and memory-saving algorithm for large-volume imaging of organoids

Weiqiang Zheng, Juan Wang, Yunhong Zhou, Qiang Zeng, Cheng Zhang, Li Liu, Hui Yu & YUTING YANG
Organoids, the 3D culture systems derived from stem cells, are promising models for human organs. But organoid study requires large-volume imaging with single cell resolution, which is beyond the spatial bandwidth limit of conventional optical microscopy. Herein, we propose a lensless holographic microscope empowered with a time and memory-saving algorithm. It solves the tradeoff between the imaging field of view, resolution and processing speed, and provides a practical tools for organoids’ study. We first build...
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