MOLECULAR CHARACTERIZATION OF THE MICROBIAL COMMUNITY INVOLVED IN THE CARBON CYCLE IN DIFFERENT AREAS AND TYPES OF SOIL MANAGEMENT

Silvia Landi, Raimondo Piccolo, Stefania Simoncini & Roberta Pastorelli
This paper addresses the diversity of two soil bacterial groups involved in the biogeochemical carbon cycle: bacteria implicated in chitin degradation and methanotrophs. To evaluate the influence of soil physico-chemical and anthropic characteristics on the diversity of these microbial groups, total DNA was directly extracted from soils differently managed and sampled in central and south Italy. PCR-Denaturing Gradient Gel Electrophoresis (DGGE) analyses targeting genes coding for chitinase (chiA), particulate methane monooxygenase (pmoA) and 16S rRNA...