MOLECULAR CHARACTERIZATION OF THE MICROBIAL COMMUNITY INVOLVED IN THE CARBON CYCLE IN DIFFERENT AREAS AND TYPES OF SOIL MANAGEMENT

Landi, Silvia; CRA-ABP Consiglio Per La Ricerca E La Sperimentazione In Agricoltura Centro Di Ricerca Per L’Agrobiologia E La Pedologia, Firenze, Piccolo, Raimondo; CRA-ABP Consiglio Per La Ricerca E La Sperimentazione In Agricoltura Centro Di Ricerca Per L’Agrobiologia E La Pedologia, Firenze, Simoncini, Stefania; CRA-ABP Consiglio Per La Ricerca E La Sperimentazione In Agricoltura Centro Di Ricerca Per L’Agrobiologia E La Pedologia, Firenze & Pastorelli, Roberta; CRA-CIN Consiglio Per La Ricerca E La Sperimentazione In Agricoltura Centro Di Ricerca Per Le Colture Industriali , Bologna
This paper addresses the diversity of two soil bacterial groups involved in the biogeochemical carbon cycle: bacteria implicated in chitin degradation and methanotrophs. To evaluate the influence of soil physico-chemical and anthropic characteristics on the diversity of these microbial groups, total DNA was directly extracted from soils differently managed and sampled in central and south Italy. PCR-Denaturing Gradient Gel Electrophoresis (DGGE) analyses targeting genes coding for chitinase (chiA), particulate methane monooxygenase (pmoA) and 16S rRNA...