Characterization of RNA in exosomes secreted by human breast cancer cell lines using next-generation sequencing

Piroon Jenjaroenpun, Yuliya Kremenska, Vrundha M Nair, Maksym Kremenskoy, Baby Joseph & Igor V Kurochkin
Exosomes are nanosized (30-100 nm) membrane vesicles secreted by most cell types. Exosomes have been found to contain various RNA species including miRNA, mRNA and long non-protein coding RNAs. A number of cancer cells produce elevated levels of exosomes. Because exosomes have been isolated from most body fluids they may provide a source for non-invasive cancer diagnostics. Transcriptome profiling that uses deep-sequencing technologies (RNA-Seq) offers enormous amount of data that can be used for biomarkers...