Orthogonality and burden of a heterologous AND gate gene circuit in E. coli host

Baojun Wang
RNA-Seq sample preparation and sequencing E. coli NCM3722 cultures were grown and growth stopped 4 hrs post day dilution by adding 1/10 volume of 5% phenol 95% ethanol (v/v). Cells were harvested by centrifugation (4500 x g for 30 min). Supernatants were discarded and pellets drained by gravity flow for 5 min. Pellets wet weights were measured by subtracting the weights of cognate empty tubes. There are 7 samples in total containing 6 different plasmid...
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