Reconstruction of image sequences from ungated and scanning-aberrated laser scanning microscopy images of the beating heart

O. Mariani, Alexander Ernst, Nadia Mercader & Michael Liebling
Fluorescence laser-scanning microscopy is a wellestablished imaging technique in biology, available in many imaging facilities to investigate structures within live animal embryos such as zebrafish. Laser scanning microscopes (LSM) are limited when used to study dynamic heart morphology or function. Despite their ability to resolve static cardiac structures, the fast motion of the beating heart introduces severe artifacts in the scanned images and gating the acquisitions to the heartbeat is difficult to implement on traditional...
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