5 Works

Data from: Cost-effective enrichment hybridization capture of chloroplast genomes at deep multiplexing levels for population genetics and phylogeography studies

Cédric Mariac, Nora Scarcelli, Juliette Pouzadou, Adeline Barnaud, Claire Billot, Adama Faye, Vincent Maillol, Sylvain Santoni, Yves Vigouroux, Thomas L. P. Couvreur, Ayite Kougbeadjo, François Sabot & Guillaume Martin
Biodiversity, phylogeography and population genetic studies will be revolutionized by access to large datasets thanks to next generation sequencing methods. In this study, we develop an easy and cost-effective protocol for in-solution enrichment hybridization capture of complete chloroplast genomes applicable at deep multiplexed levels. The protocol uses cheap in-house species-specific probes developed via long range PCR of the entire chloroplast. Barcoded libraries are constructed and in-solution enrichment of the chloroplasts is done using the probes....

Data from: Bushmeat genetics: setting up a reference framework for the DNA-typing of African forest bushmeat

Philippe Gaubert, Flobert Njiokou, Ayodeji Olayemi, Paolo Pagani, Sylvain Dufour, Emmanuel Danquah, Mac Elikem K. Nutsuakor, Gabriel Ngua, Alain-Didier Missoup, Pablo A. Tedesco, Rémy Dernat & Agostinho Antunes
The bushmeat trade in tropical Africa represents illegal, unsustainable off-takes of millions of tons of wild game – mostly mammals – per year. We sequenced four mitochondrial gene fragments (cyt b, COI, 12S, 16S) in >300 bushmeat items representing nine mammalian orders and 59 morphological species from five western and central African countries (Guinea, Ghana, Nigeria, Cameroon and Equatorial Guinea). Our objectives were to assess the efficiency of cross-species PCR amplification and to evaluate the...

Data from: Closing a gap in tropical forest biomass estimation: taking crown mass variation into account in pantropical allometries

Pierre Ploton, Nicholas Barbier, Stéphane Takoudjou Momo, Maxime Réjou-Méchain, Faustin Boyemba Bosela, Georges Chuyong, Gilles Dauby, Vincent Droissart, Adeline Fayolle, Rosa Calisto Goodman, Mathieu Henry, Narcisse Guy Kamdem, John Katembo Mukirania, David Kenfack, Moses Libalah, Alfred Ngomanda, Vivien Rossi, Bonaventure Sonké, Nicolas Texier, Duncan Thomas, Donatien Zebaze, Pierre Couteron, Uta Berger & Raphaël Pélissier
Accurately monitoring tropical forest carbon stocks is an outstanding challenge. Allometric models that consider tree diameter, height and wood density as predictors are currently used in most tropical forest carbon studies. In particular, a pantropical biomass model has been widely used for approximately a decade, and its most recent version will certainly constitute a reference in the coming years. However, this reference model shows a systematic bias for the largest trees. Because large trees are...

Data from: Intra-individual polymorphism in chloroplasts from NGS data: where does it come from and how to handle it?

N. Scarcelli, C. Mariac, T. L. P. Couvreur, A. Faye, D. Richard, F. Sabot, C. Berthouly-Salazar & Y. Vigouroux
Next generation sequencing allows access to a large quantity of genomic data. In plants, several studies used whole chloroplast genome sequences for inferring phylogeography or phylogeny. Even though the chloroplast is a haploid organelle, NGS plastome data identified a non negligible number of intra-individual polymorphic SNPs. Such observations could have several causes such as sequencing errors, the presence of heteroplasmy or transfer of chloroplast sequences in the nuclear and mitochondrial genomes. The occurrence of allelic...

Cocoa mirid attractiveness by different colour of trap

Hermine Mahot, Joseph R. Mahob, David R. Hall, Sarah E.J. Arnold, Apollin K. Fotso, Gertrude Membang, Nathalie Ewane, Adolph Kemga, Komi K.M. Fiaboe, Charles F.B. Bilong & Rachid Hanna
Cocoa mirids, Sahlbergella singularis and Distantiella theobroma, are the most economically important insect pests of cocoa in West and Central Africa, where they can cause up to 40% losses in cocoa yields. Sahlbergella singularis is the most common species in Cameroon and was therefore targeted in this study. During a two-year trial in 15 cocoa plantations in Ayos and Konye, in the Centre and Southwest regions of Cameroon respectively, the most effective colour – white,...

Registration Year

  • 2014
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  • 2015
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  • 2016
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Resource Types

  • Dataset
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Affiliations

  • Université de Yaoundé I
    5
  • Institut de Recherche pour le Développement
    2
  • University of Liège
    1
  • University of Greenwich
    1
  • Kwame Nkrumah University of Science and Technology
    1
  • Oregon State University
    1
  • Université Libre de Bruxelles
    1
  • French National Institute for Agricultural Research
    1
  • International Institute of Tropical Agriculture
    1
  • International Institute of Tropical Agriculture
    1
  • Yale University
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  • Harvard University
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  • University of Buea
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  • TU Dresden
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  • University of Porto
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