4 Works

Data from: High fidelity detection of crop biomass QTL from low-cost imaging in the field

Darshi Banan, Rachel E. Paul, Max J. Feldman, Mark W. Holmes, Hannah Schlake, Ivan Baxter, Hui Jiang & Andrew D.B. Leakey
Field-based, rapid, and non-destructive techniques for assessing plant productivity are needed to accelerate the discovery of genotype-to-phenotype relationships in next-generation biomass grass crops. The use of hemispherical imaging and light attenuation modeling was evaluated against destructive harvest measures with respect to their ability to accurately capture phenotypic and genotypic relationships in a field-grown grass crop. Plant area index (PAI) estimated from below-canopy hemispherical images, as well as a suite of thirteen traits assessed by manual...

Data from: Robust DNA isolation and high-throughput sequencing library construction for herbarium specimens

Saman Saeidi, Michael R. McKain & Elizabeth A. Kellogg
Herbaria are an invaluable source of plant material that can be used in a variety of biological studies. The use of herbarium specimens is associated with a number of challenges including sample preservation quality, degraded DNA, and destructive sampling of rare specimens. In order to more effectively use herbarium material in large sequencing projects, a dependable and scalable method of DNA isolation and library preparation is needed. This paper demonstrates a robust, beginning-to-end protocol for...

Data from: Brassicales phylogeny inferred from 72 plastid genes: a reanalysis of the phylogenetic localization of two paleopolyploid events and origin of novel chemical defenses

Patrick P. Edger, Jocelyn C. Hall, Alex Harkess, Michelle Tang, Jill Coombs, Setareh Mohammadin, M. Eric Schranz, Zhiyong Xiong, James Leebens-Mack, Blake C. Meyers, Kenneth J. Systma, Marcus A. Koch, Ihsan A. Al-Shehbaz, J. Chris Pires & Kenneth J. Sytsma
PREMISE OF THE STUDY - Previous phylogenetic studies employing molecular markers have yielded various insights into the evolutionary history across Brassicales, but many relationships between families remain poorly supported or unresolved. A recent phylotranscriptomic approach utilizing 1155 nuclear markers obtained robust estimates for relationships among 14 of 17 families. Here we report a complete family‐level phylogeny estimated using the plastid genome. METHODS - We conducted phylogenetic analyses on a concatenated data set comprising 44,926 bp...

Data from: Specimen-based analysis of morphology and the environment in ecologically dominant grasses: the power of the herbarium

Christine A. McAllister, Michael R. McKain, Mao Li, Bess Bookout & Elizabeth A. Kellogg
Herbaria contain a cumulative sample of the world’s flora, assembled by thousands of people over several hundred years. Recent advances in computation, DNA sequencing, and image manipulation have allowed us to capitalize on this resource. Using herbarium material, we conducted a species-level analysis of a major clade in the grass tribe Andropogoneae, which includes the dominant species of the world’s grasslands, from the genera Andropogon, Schizachyrium, Hyparrhenia, and other groups. We imaged 188 of the...

Registration Year

  • 2018
    4

Resource Types

  • Dataset
    4

Affiliations

  • Donald Danforth Plant Science Center
    4
  • University of Georgia
    1
  • Inner Mongolia University
    1
  • University of Alberta
    1
  • University of Wisconsin-Madison
    1
  • Principia College
    1
  • University of Missouri
    1
  • Heidelberg University
    1
  • University of Alabama, Tuscaloosa
    1
  • University of Illinois at Urbana Champaign
    1