9 Works

Supplementary mass spectonomy data to analyse generation of spliced mutant Kras/RAC2 epitopes

Gerald Willimsky
In vitro proteasome processing of neo-splicetopes does not predict their presentation in vivo Proteasome catalyzed peptide splicing (PCPS) of cancer-driving antigens could generate attractive neoepitopes to be targeted by TCR-based adoptive T cell therapy. Based on a spliced peptide prediction algorithm TCRs were generated against putative KRASG12V and RAC2P29L derived neo-splicetopes with high HLA-A*02:01 binding affinity. TCRs generated in mice with a diverse human TCR repertoire specifically recognized the respective target peptides with high efficacy....

Current Advances, Challenges, and Opportunities in Stroke Research, Management, and Care

Julius Emmrich, Samuel Knauss & Matthias Endres
Stroke is the second leading cause of death (after heart disease) and a major cause of disability worldwide. Also called a brain attack, typical symptoms of stroke include sudden one-sided weakness and numbness in the face and arm as well as altered speech. Most strokes are ischaemic strokes where arterial blood flow to the brain is interrupted by an embolism or thrombosis. Strokes are less frequently caused by haemorrhage. Non-contrast cranial computed tomography (CT) imaging,...

Data from: Clinical presentation and management of SMART syndrome

Sebastian F. Winter, Joshua P. Klein, Eugene J. Vaios, Philipp Karschnia, Eudocia Q. Lee, Helen A. Shih, Franziska Loebel & Jorg Dietrich
Stroke-like migraine attacks after radiation therapy (SMART) syndrome represents a rare but serious condition manifesting years after cranial radiation therapy (RT). Characterized by migraine-type headaches, stroke-like deficits and/or seizures and MR imaging abnormalities, including cortical gyriform enhancement in irradiated brain regions, SMART remains diagnostically and therapeutically challenging. Distinction from tumor progression is difficult and treatment options are limited. Although frequently reversible, SMART episodes can recur and effectuate persistent neurologic and/or imaging sequelae.

A comprehensive survey among statistical members of medical ethics committees in Germany on their personal impression of completeness and correctness of biostatistical aspects of submitted study protocols

Geraldine Rauch
Original data of a comprehensive survey among statistical members of medical ethics committees in Germany on their personal impression of completeness and correctness of biostatistical aspects of submitted study protocols. To enable reproduction of the results presented in the corresponding paper, the final dataset is freely available here, without any information on the medical ethics committee’s federal state to avoid any risk of potential re-identification.

Sepsis geht alle an!

Rainer Petzina, Herwig Gerlach, Manuela Forster, Christiane Hartog & Matthias Gründling

Data for: Effect of using a cognitive aid on content and feasibility of debriefings of simulated emergencies

Julia Freytag, Fabian Stroben, Wolf Hautz, Dorothea Penders & Juliane Kämmer
Background Adverse events in patient care are often caused by failures in teamwork. Simulation training and its debriefing can contribute to improving teamwork and thus patient care. When conducting debriefings, there are several design factors that can potentially influence learning outcomes. This study examines the use of a cognitive aid to help structure the content of debriefings and compares it with debriefings that are merely roughly structured. In addition, the feasibility of the debriefing, the...

Prospective Quantification of CSF Biomarkers in Antibody-Mediated Encephalitis

Gregory Day, Melanie L. Yarbrough, Peter Körtvelyessy, Harald Prüss, Robert C. Bucelli, Marvin J. Fritzler, Warren Mason, David F. Tang-Wai, Claude Steriade, Julien Hébert, Rachel L. Henson, Elizabeth M. Herries, Jack H. Ladenson, A. Sebastian Lopez-Chiriboga, Neill R. Graff-Radford, John C. Morris & Anne Fagan
Objective: To determine whether neuronal and neuroaxonal injury, neuroinflammation, and synaptic dysfunction associate with clinical course and outcomes in antibody-mediated encephalitis (AME), we measured biomarkers of these processes in CSF from patients presenting with AME and cognitively normal individuals. Methods: Biomarkers of neuronal (total tau, VILIP-1) and neuroaxonal damage (neurofilament light chain [NfL]), inflammation (YKL-40), and synaptic function (neurogranin, SNAP-25) were measured in CSF obtained from 45 patients at the time of diagnosis of NMDA...

Appendix: Association between hemostatic profile and migraine: a Mendelian randomization analysis

Yanjun Guo, Pamela Rist, Maria Sabater-Lleal, Paul De Vries, Nicholas Smith, Paul Ridker, Tobias Kurth & Daniel Chasman
Objective: To assess support for a causal relationship between hemostatic measures and migraine susceptibility using genetic instrumental analysis. Methods: Two-sample Mendelian randomization (MR) instrumental leveraging available genome-wide association study (GWAS) summary statistics was applied to hemostatic measures as potential causal for migraine and its subtypes, migraine with aura (MA) and migraine without aura (MO). Twelve blood-based measures of hemostasis were examined, including plasma level or activity of eight hemostatic factors and two fibrinopeptides together with...

Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stages

Carolin Ulbricht, Ruth Leben, Asylkhan Rakhymzhan, Frank Kirchhoff, Lars Nitschke, Helena Radbruch, Raluca Niesner & Anja Hauser
Calcium is a universal second messenger present in all eukaryotic cells. The mobilization and storage of Ca2+ ions drives a number of signaling-related processes, stress-responses or metabolic changes, all of which are relevant for the development of immune cells and their adaption to pathogens. Here, we introduce the FRET-reporter mouse YellowCaB expressing the genetically encoded calcium indicator TN-XXL in B lymphocytes. Calcium-induced conformation change of TN-XXL results in FRET-donor quenching measurable by two-photon fluorescence lifetime...

Registration Year

  • 2021
    9

Resource Types

  • Dataset
    7
  • Text
    2

Affiliations

  • Charité
    7
  • Charité - University Medicine Berlin
    2
  • Brigham and Women's Hospital
    2
  • Massachusetts General Hospital
    1
  • New York University Langone Medical Center
    1
  • Universitätsklinik für Neurologie
    1
  • University of Washington
    1
  • University of Erlangen-Nuremberg
    1
  • German Rheumatism Research Centre
    1
  • Saarland University
    1