220 Works

Data from: Phylogeography and demographic history of two widespread Indo-Pacific mudskippers (Gobiidae: Periophthalmus)

Gianluca Polgar, Lorenzo Zane, Massimiliano Babbucci, Federica Barbisan, Tomaso Patarnello, Lukas Rüber & Chiara Papetti
This study provides a first description of the phylogeographic patterns and evolutionary history of two species of the mudskipper genus Periophthalmus. These amphibious gobies are distributed throughout the whole Indo-Pacific region and Atlantic coast of Africa, in peritidal habitats of soft-bottom coastal ecosystems. Three sequence datasets of two widely distributed species, Periophthalmus argentilineatus and P. kalolo, were obtained by amplifying and sequencing two mtDNA markers (D-loop and 16S rDNA) and the nDNA rag1 region. The...

Data from: \"Transcriptome sequences for Campanula gentilis\" in Genomic Resources Notes accepted 1 April 2015 – 31 May 2015

Töre Demet, Federico Luebert, Guilhem Mansion, Ludo A. H. Muller, M. Vidotto, E. Boscari, L. Congiu, A. Grapputo, L. Zane, Vera Maria Fonseca Almeida-Val, Maria Manuela Coelho, Tiago Filipe Jesus & Demet Töre
In this report, we present the transcriptome of a single accession of Campanula gentilis Kovanda, obtained through the sequencing of both a normalized and a non-normalized cDNA library generated from stem and leaf tissue. The resources we provide include the raw sequence reads, the assembled contigs, the putative open reading frames, the contig/ORF annotations and the normalized as well as non-normalized expression levels.

Data from: High cover of hedgerows in the landscape supports multiple ecosystem services in Mediterranean cereal fields

Matteo Dainese, Silvia Montecchiari, Tommaso Sitzia, Maurizia Sigura & Lorenzo Marini
Field-margin diversification through conservation and restoration of hedgerows is becoming a prominent intervention for promoting biodiversity and associated ecosystem services in intensive agricultural landscapes. However, how increasing cover of hedgerows in the landscape can affect ecosystem services has rarely been considered. Here, we assessed the effect of increased field-margin complexity at the local scale and increasing cover of hedgerows in the landscape on the provision of pest control, weed control and potential pollination. Locally, three...

Data from: The replication of Frataxin gene is assured by activation of dormant origins in the presence of a GAA-repeat expansion

Martina Stevanoni, Elisa Palumbo & Antonella Russo
It is well known that DNA replication affects the stability of several trinucleotide repeats, but whether replication profiles of human loci carrying an expanded repeat differ from those of normal alleles is poorly understood in the endogenous context. We investigated this issue using cell lines from Friedreich’s ataxia patients, homozygous for a GAA-repeat expansion in intron 1 of the Frataxin gene. By interphase, FISH we found that in comparison to the normal Frataxin sequence the...

Data from: Familiarity mediates equitable social associations in guppies

Silvia Cattelan, Tyrone Lucon-Xiccato, Andrea Pilastro & Matteo Griggio
Several fish species are characterized by social systems in which individuals prefer to interact with specific group mates. However, there is no clear evidence that such associations are equitable, i.e. they are mutual between individuals. We addressed this issue in the guppy (Poecilia reticulata), a species in which social network analyses have evidenced non-random social associations between individuals. We measured the mutual preference in pairs of guppies that previously familiarized in groups of either two...

Data from: Quick-change artists: male guppies pay no cost to repeatedly adjust their sexual strategies

Martina Magris, Gianluca Chimetto, Sofia Rizzi & Andrea Pilastro
Sexually selected traits involved in mate acquisition and fertilization success are usually costly and males often plastically adjust their reproductive investment in response to social conditions. Phenotypic plasticity in male sexual traits is generally assumed to be adaptive, yet its costs are rarely investigated. Male guppies (Poecilia reticulata) adjust their ejaculate production and sexual behavior in response to perceived mating opportunities. In natural populations mating opportunities can fluctuate continuously, and the iterated activation of plastic...

Data from: The interplay of landscape composition and configuration: new pathways to manage functional biodiversity and agro-ecosystem services across Europe

Emily A. Martin, Matteo Dainese, Yann Clough, András Báldi, Riccardo Bommarco, Vesna Gagic, Michael Garratt, Andrea Holzschuh, David Kleijn, Anikó Kovács-Hostyánszki, Lorenzo Marini, Simon G. Potts, Henrik G. Smith, Diab Al Hassan, Matthias Albrecht, Georg K. S. Andersson, Josep Asis, Stephanie Aviron, Mario Balzan, Laura Baños-Picón, Ignasi Bartomeus, Peter Batary, Françoise Burel, Berta Caballero-López, Elena D. Concepcion … & Ingolf Steffan-Dewenter
Managing agricultural landscapes to support biodiversity and ecosystem services is a key aim of a sustainable agriculture. However, how the spatial arrangement of crop fields and other habitats in landscapes impacts arthropods and their functions is poorly known. Synthesising data from 49 studies (1515 landscapes) across Europe, we examined effects of landscape composition (% habitats) and configuration (edge density) on arthropods in fields and their margins, pest control, pollination and yields. Configuration effects interacted with...

Data from: Behavioural patterns of vocal greeting production in four primate species

Pawel Fedurek, Christof Neumann, Yaelle Bouquet, Stephanie Mercier, Martina Magris, Fredy Quintero & Klaus Zuberbühler
Social animals have evolved a range of signals to avoid aggressive and facilitate affiliative interactions. Vocal behaviour is especially important in this respect with many species, including various primates, producing acoustically distinct ‘greeting calls’ when two individuals approach each other. While the ultimate function of greeting calls has been explored in several species, little effort has been made to understand the mechanisms of this behaviour across species. The aim of this study was to explore...

Data from: Extra food provisioning reduces extra-pair paternity in the lesser kestrel (Falco naumanni)

Alessandra Costanzo, Nicola Tommasi, Andrea Galimberti, Giorgia Scesa, Roberto Ambrosini, Matteo Griggio, Jacopo G. Cecere & Diego Rubolini
Female promiscuity can function to acquire both direct and indirect benefits from their social mate and extra-pair males. In many raptor species, intense mate-feeding significantly contributes to female energy requirements before and during egg laying. Moreover, females may use mate-feeding effort to assess male quality. In this study of the lesser kestrel (Falco naumanni), we aimed at experimentally manipulating the female’s perception of mate quality by providing females with extra food during egg laying, and...

Along-shelf connectivity and circumpolar gene flow in Antarctic silverfish (Pleuragramma antarctica)

Jilda Alicia Caccavo, Chiara Papetti, , Rainer Knust, Julian R. Ashford & Lorenzo Zane
The Antarctic silverfish (Pleuragramma antarctica) is a critically important forage species with a circumpolar distribution and is unique among other notothenioid species for its wholly pelagic life cycle. Previous studies have provided mixed evidence of population structure over regional and circumpolar scales. The aim of the present study was to test the recent population hypothesis for Antarctic silverfish, which emphasizes the interplay between life history and hydrography in shaping connectivity. A total of 1067 individuals...

Simultaneous two-photon voltage or calcium imaging and multi-channel LFP recordings in barrel cortex of awake and anesthetized mice

Claudia Cecchetto, Stefano Vassanelli & Bernd Kuhn
Neuronal population activity, both spontaneous and sensory-evoked, generates propagating waves in cortex. However, high spatiotemporal-resolution mapping of these waves is difficult as calcium imaging, the work horse of current imaging, does not reveal subthreshold activity. Here, we present a platform combining voltage or calcium two-photon imaging with multi-channel local field potential (LFP) recordings in different layers of the barrel cortex from anesthetized and awake head-restrained mice. A chronic cranial window with access port allows injecting...

Italy

Claudia Padovani, Giuliano Bobba, Alice Baroni, Marinella Belluati, Cecilia Biancalana, Mauro Bomba, Alice Fubini, Francesco Marrazzo, Rossella Rega, Christian Ruggiero, Simone Sallusti, Sergio Splendore & Michele  Valente

L’icnosito della località “Foresta” di Tora e Piccilli e le impronte umane fossili

Adolfo Panarello, Gennaro Farinaro & Paolo Mietto
This report provides a complete description of the general “Ciampate del diavolo” palaeontological site (Tora e Piccilli, Central-Southern Italy) and each of the human ichnites there found so far. This description is integrated and completed with the tables, graphs, and pictures given in Supplements 1, 2, and 3 of this volume [Panarello et al., 2022a;b;c]. We think that this huge amount of both numeric and visual data will be very useful for any other human...

Segni umani recenti sulla pendice delle orme preistoriche

Paolo Mietto & Adolfo Panarello
The various on-field activities which have been carried out by researchers in the “Devil’s Trails” palaeontological site from 2005 to 2010 and the subsequent Ph.D. researches on the site that took place until 2016 have allowed us to identify - in addition to the various ichnological evidence of prehistoric times - also a series of anthropic cuts made in various historical times. These cuts sometimes overlap the prehistoric traces, or partially alter them, so creating...

Mnamon: Cuneiforme sumerico

Salvatore Gaspa
Introduzione critica al cuneiforme sumerico, risorse elettroniche e bibliografia.

Synaptic alterations in visual cortex reshape contrast-dependent gamma oscillations and inhibition-excitation ratio in a genetic mouse model of migraine

Nicolò Meneghetti, Chiara Cerri, Eleonora Vannini, Elena Tantillo, Angelita Tottene, Daniela Pietrobon, Matteo Caleo & Alberto Mazzoni
Abstract Background Migraine affects a significant fraction of the world population, yet its etiology is not completely understood. In vitro results highlighted thalamocortical and intra-cortical glutamatergic synaptic gain-of-function associated with a monogenic form of migraine (familial-hemiplegic-migraine-type-1: FHM1). However, how these alterations reverberate on cortical activity remains unclear. As altered responsivity to visual stimuli and abnormal processing of visual sensory information are common hallmarks of migraine, herein we investigated the effects of FHM1-driven synaptic alterations in...

sj-pdf-1-pss-10.1177_09567976221097947 – Supplemental material for Severe Developmental Dyscalculia Is Characterized by Core Deficits in Both Symbolic and Nonsymbolic Number Sense

Gisella Decarli, Francesco Sella, Silvia Lanfranchi, Giulia Gerotto, Silvia Gerola, Giuseppe Cossu & Marco Zorzi
Supplemental material, sj-pdf-1-pss-10.1177_09567976221097947 for Severe Developmental Dyscalculia Is Characterized by Core Deficits in Both Symbolic and Nonsymbolic Number Sense by Gisella Decarli, Francesco Sella, Silvia Lanfranchi, Giulia Gerotto, Silvia Gerola, Giuseppe Cossu and Marco Zorzi in Psychological Science

Additional file 4 of LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer

Donatella Delle Cave, Silvia Buonaiuto, Bruno Sainz, Marco Fantuz, Maria Mangini, Alessandro Carrer, Annalisa Di Domenico, Tea Teresa Iavazzo, Gennaro Andolfi, Carme Cortina, Marta Sevillano, Christopher Heeschen, Vincenza Colonna, Marco Corona, Antonio Cucciardi, Martina Di Guida, Eduard Batlle, Annachiara De Luca & Enza Lonardo
Additional file 4: Figure S4. Knockdown of LAMC2 does not affect cell growth. (a) Western blot analysis of LAMC2 in sh empty and LAMC2 knockdown cells. Parallel β-ACTIN immunoblotting was performed. (b) qPCR analysis for LAMC2 and CD44 gene expression in sh empty and LAMC2 knockdown cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to sh empty. (c) Representative images of sh empty and LAMC2 knockdown cells...

Additional file 6 of LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer

Donatella Delle Cave, Silvia Buonaiuto, Bruno Sainz, Marco Fantuz, Maria Mangini, Alessandro Carrer, Annalisa Di Domenico, Tea Teresa Iavazzo, Gennaro Andolfi, Carme Cortina, Marta Sevillano, Christopher Heeschen, Vincenza Colonna, Marco Corona, Antonio Cucciardi, Martina Di Guida, Eduard Batlle, Annachiara De Luca & Enza Lonardo
Additional file 6: Figure S6. Knockdown of LAMC2 affects tumorigenicity. (a) Representative images from a gelatin degradation assay of sh empty and LAMC2 knockdown cells. Nuclei were stained with Hoechst 33342 (blue), green represents actin (Alexa Fluor™ 488 Phalloidin) and red illustrates gelatin (Rodhamine). The white dashed line circles indicate the areas of degradation. (b) Degradation potential of sh empty and LAMC2 knockdown cells. (c) qPCR analysis for EMT genes in sh empty and LAMC2...

Additional file 10 of LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer

Donatella Delle Cave, Silvia Buonaiuto, Bruno Sainz, Marco Fantuz, Maria Mangini, Alessandro Carrer, Annalisa Di Domenico, Tea Teresa Iavazzo, Gennaro Andolfi, Carme Cortina, Marta Sevillano, Christopher Heeschen, Vincenza Colonna, Marco Corona, Antonio Cucciardi, Martina Di Guida, Eduard Batlle, Annachiara De Luca & Enza Lonardo
Additional file 10: Figure S10. Inhibition of transforming growth factor beta (TGF-β) signaling blocks LAMC2-induced metastasis. (a) Enrichment plots for pancreatic cancer, focal adhesion, hypoxia, MAPK signaling, glycolysis and gluconeogenesis pathways in EGFP+ versus EGFP- cells isolated by FACS from subcutaneous tumors. (b) Western blot analysis for pSMAD2 and SMAD2 in EGFP- versus EGFP+ PANC-1 and #253 cells. Parallel GAPDH immunoblotting was performed. (c) Flow cytometry quantification of EGFP and ALK5-TGFβR1 in EGFP- and EGFP+...

sj-docx-1-dhj-10.1177_20552076221133696 - Supplemental material for A topic trend analysis on COVID-19 literature

Sara Urru, Veronica Sciannameo, Corrado Lanera, Silvano Salaris, Dario Gregori & Paola Berchialla
Supplemental material, sj-docx-1-dhj-10.1177_20552076221133696 for A topic trend analysis on COVID-19 literature by Sara Urru, Veronica Sciannameo, Corrado Lanera, Silvano Salaris, Dario Gregori and Paola Berchialla in Digital Health

Additional file 3 of Unlocking the molecular basis of wheat straw composition and morphological traits through multi-locus GWAS

Salvatore Esposito, Francesca Taranto, Paolo Vitale, Donatella Bianca Maria Ficco, Salvatore Antonio Colecchia, Piergiorgio Stevanato & Pasquale De Vita
Additional file 3: Supplementary Fig. 3. Genotypic variability of the 185wheat genotypes. Loading plot of the first (PC1) and second (PC2) principalcomponents showing the variation among individuals. Based on Triticum ssp.,genotypes are represented by different colored symbols indicated in the legend.

Additional file 1 of Unlocking the molecular basis of wheat straw composition and morphological traits through multi-locus GWAS

Salvatore Esposito, Francesca Taranto, Paolo Vitale, Donatella Bianca Maria Ficco, Salvatore Antonio Colecchia, Piergiorgio Stevanato & Pasquale De Vita
Additional file 1: Supplementary Fig. 1. Frequency distribution of BLUPsfor 15 traits among cultivars. X-axisshow BLUP values while in the y-axisthe frequency is reported.

Additional file 4 of Unlocking the molecular basis of wheat straw composition and morphological traits through multi-locus GWAS

Salvatore Esposito, Francesca Taranto, Paolo Vitale, Donatella Bianca Maria Ficco, Salvatore Antonio Colecchia, Piergiorgio Stevanato & Pasquale De Vita
Additional file 4: Supplementary Fig. 4. Intra-chromosomal LD decaydistance (kb) evaluated considering (A) the whole genome, (B) A genome, (C) Bgenome. Dashed lines indicate the r2 threshold. The intersectionpoint between the decay LD curve and the LD threshold was shown.

Additional file 7 of Unlocking the molecular basis of wheat straw composition and morphological traits through multi-locus GWAS

Salvatore Esposito, Francesca Taranto, Paolo Vitale, Donatella Bianca Maria Ficco, Salvatore Antonio Colecchia, Piergiorgio Stevanato & Pasquale De Vita
Additional file 7: Supplementary Table 1. BLUPs values for the traits under study.

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