Characterization and mutagenesis of Chinese hamster ovary cells endogenous retroviruses to inactivate viral particle release

Pierre-Olivier Duroy, Sandra Bosshard, Emanuel Schmid‐Siegert, Samuel Neuenschwander, Ghislaine Arib, Philippe Lemercier, Jacqueline Masternak, Lucien Roesch, Flavien Buron, Pierre‐Alain Girod, Ioannis Xenarios & Nicolas Mermod
The Chinese hamster ovary (CHO) cells used to produce biopharmaceutical proteins are known to contain type‐C endogenous retrovirus (ERV) sequences in their genome and to release retroviral‐like particles. Although evidence for their infectivity is missing, this has raised safety concerns. As the genomic origin of these particles remained unclear, we characterized type‐C ERV elements at the genome, transcriptome, and viral particle RNA levels. We identified 173 type‐C ERV sequences clustering into three functionally conserved groups....

Registration Year

  • 2019
    1

Resource Types

  • Dataset
    1

Affiliations

  • Swiss Institute of Bioinformatics
    1
  • University of Lausanne
    1