Additional file 2: Table S1. Statistical analysis of behavioural bioassays. Two-way ANONA method was used to evaluate the significance of the behavioural modifications.

PLEASE NOTE, THESE DATA ARE ALSO REFERRED TO IN ANOTHER PUBLICATION. PLEASE SEE http://dx.doi.org/10.1111/mec.14493. The fungal skin disease chytridiomycosis has caused the devastating decline and extinction of hundreds of amphibian species globally, yet the potential for evolving resistance, and the underlying pathophysiological mechanisms remain poorly understood. We exposed 406 naïve, captive-raised alpine tree frogs (Litoria verreauxii alpina) from multiple populations (one evolutionarily naïve to chytridiomycosis) to the aetiological agent Batrachochytrium dendrobatidis in two concurrent and...

Because little is known about cataract in pilots, we estimated prevalence by anonymously ascertaining all commercial airline pilots diagnosed with cataract 2011–2016 using the electronic Medical Records System of the Australian Civil Aviation Safety Authority. Of 14,163 Australian male commercial pilots licensed in 2011, 1286 aged ≥60 had biennial eye examinations showing a cataract prevalence of 11.6%. Among 12,877 pilots aged <60, based on compulsory eye examinations only when first licensed, prevalence was 0.5%. There...

Diagnosis of attention deficit hyperactive disorder (ADHD) has been associated with increased risk of mortality in large register samples. However, there is less known about the association between symptoms of ADHD in adolescents and risk of mortality in general population samples. The Northern Finland Birth Cohort 1986 (n = 9432 at recruitment in early pregnancy) linked to nationwide register data for deaths was utilized to study the association between parent-rated ADHD symptoms assessed using Strengths...

Additional file 2.

Abstract Background Observational epidemiological studies suggest a link between several factors related to ovulation and reproductive function and endometrial cancer (EC) risk; however, it is not clear whether these relationships are causal, and whether the risk factors act independently of each other. The aim of this study was to investigate putative causal relationships between the number of live births, age at last live birth, and years ovulating and EC risk. Methods We conducted a series...

Latex for a supplemental document with expanded experimental methods

Additional file 1: Figure S1. ECF showed no effect on mice with DSS-induced colitis. ECF was intraperitoneally injected into mice, which were then administered DSS for 7 days. The differences in parameters between the DSS and ECF-DSS groups are shown: (A) body weight change, (B) DAI score, and (C) colon length (8–10 mice per group). The experiment was repeated independently.

Additional file 1: Figure S1. ECF showed no effect on mice with DSS-induced colitis. ECF was intraperitoneally injected into mice, which were then administered DSS for 7 days. The differences in parameters between the DSS and ECF-DSS groups are shown: (A) body weight change, (B) DAI score, and (C) colon length (8–10 mice per group). The experiment was repeated independently.

Supplemental material, sj-docx-2-dhj-10.1177_20552076221144104 for Understanding what we know so far about young people's engagement with wellbeing apps. A scoping review and narrative synthesis by Gaston Antezana, Anthony Venning, David Smith and Niranjan Bidargaddi in Digital Health

Cancers require telomere maintenance mechanisms for unlimited replicative potential. They achieve this through TERT activation or alternative telomere lengthening associated with ATRX or DAXX loss. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, we dissect whole-genome sequencing data of over 2500 matched tumor-control samples from 36 different tumor types aggregated within the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium to characterize the genomic footprints of these mechanisms. While the...

Successful gene therapy for brain tumors are often limited by two important factors, the existence of blood brain barrier (BBB) and inefficient transfection of brain tumor cells. In this study, we designed a series of peptide-based gene delivery vectors decorated with T7 segment for binding the transferrin (Tf) receptors which were highly expressed on brain tumor cells, and evaluated their ability of gene delivery. The physicochemical properties of peptide vectors or peptide/DNA complexes were studied...

Massively parallel sequencing a small proportion of the whole genome at high coverage enables answering a wide range of questions from molecular evolution and evolutionary biology to animal and plant breeding and forensics. In this study, we describe the development of restriction-site associated DNA (RAD) sequencing approach for Ion Torrent PGM platform. Our protocol results in extreme genome complexity reduction using two rare-cutting restriction enzymes and strict size selection of the library allowing sequencing of...

Additional file 1: Database S1. The B. glabrata protein database used in the proteomic data analysis.

The ability to solve cognitive tasks depends upon adaptive changes in the organization of whole-brain functional networks. However, the link between task-induced network reconfigurations and their underlying energy demands is poorly understood. We address this by multimodal network analyses integrating functional and molecular neuroimaging acquired concurrently during a complex cognitive task. Task engagement elicited a marked increase in the association between glucose consumption and functional brain network reorganization. This convergence between metabolic and neural processes...

Latex for a supplemental document with expanded experimental methods

Multi-omics datasets represent distinct aspects of the central dogma of molecular biology. Such high-dimensional molecular profiles pose challenges to data interpretation and hypothesis generation. ActivePathways is an integrative method that discovers significantly enriched pathways across multiple datasets using statistical data fusion, rationalizes contributing evidence and highlights associated genes. As part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2658 cancers across 38 tumor types, we integrated...

Additional file 10: Figure S3. The co-expression levels of S. mansoni proteins identified.

Additional file 7: Figure S2. The representative MS/MS of supporting peptides of S. mansoni protein identification.

Additional file 1: Table S1. Genome-wide significant independent SNPs for Body Mass Index in women (Pulit 2018). Table S2. Genome-wide significant independent SNPs for Years Ovulating (Hg19). Table S3. Genome-wide significant independent SNPs for age at menarche downloaded from the ReproGen Consortium Website (Perry et al., 2014; Hg19). Table S4. Genome-wide significant independent SNPs for age at menopause downloaded from the ReproGen Consortium Website (Hg19; Day et al., 2015). Table S5. Genome-wide significant independent SNPs...

Additional file 2.

Additional file 3.

Abstract Background Observational epidemiological studies suggest a link between several factors related to ovulation and reproductive function and endometrial cancer (EC) risk; however, it is not clear whether these relationships are causal, and whether the risk factors act independently of each other. The aim of this study was to investigate putative causal relationships between the number of live births, age at last live birth, and years ovulating and EC risk. Methods We conducted a series...

BackgroundIncreased levels of wellbeing contribute to people being more productive, resilient, physically healthy and showing lower levels of mental illness. Using mobile apps to increase wellbeing in young people is becoming the method of choice. This study sought to critically appraise the current evidence base with regards to young people's (16–24 years of age) engagement with wellbeing apps.MethodsA systematic review of the literature and narrative synthesis was conducted to investigate users’ characteristics and other potential...

Disease progression could be altered or even reversed in decompensated patients with HBV-related cirrhosis once they initiate antiviral therapy. However, little is known about the stable re-compensation in these patients. In this retrospective study, HBV-related liver cirrhosis patients were consecutively enrolled at the first decompensated event of ascites or variceal hemorrhage (VH), and divided into immediate-treatment, on-treatment and delayed/no treatment groups. Patients were followed up to at least presence of second decompensation event or to...