Additional file 1: Supplement I. Supplemental figure 1. Lung regions used for lung ultrasound examination. A. supine position. Each hemithorax is divided in to six regions: upper and lower parts of the anterior, lateral and posterior chest wall. B: prone position. Each hemithorax is divided in to six regions: upper and lower parts of the posterior, lateral and anterior chest wall. Adapted from Heldeweg et al. with permission of the author (1). Supplement II. Definitions...

Supplementary Material 2 Illumina TSO500 NGS Gene List

Abstract Background A subset of meningiomas progress in histopathological grade but drivers of progression are poorly understood. We aimed to identify somatic mutations and copy number alterations (CNAs) associated with grade progression in a unique matched tumour dataset. Methods Utilising a prospective database, we identified 10 patients with meningiomas that had undergone grade progression and for whom matched pre- and post-progression tissue (n = 50 samples) was available for targeted next-generation sequencing. Results Mutations in...

Abstract Background Aging skin is characterized by a disturbed structure and lack of blood supply, which makes it difficult to heal once injured. ADSCs secrete large amounts of cytokines, which promote wound healing and vascular regeneration through paracrine secretion, and the number of cytokines can be elevated by hypoxic pretreating. However, the components of ADSCs are difficult to retain in wounds. Gelatin methacrylate (GelMA) is a photopolymerizable hydrogel synthesized from gelatin and has recently emerged...

Additional file 1. ASCOT ADAPT Statistical Analysis Appendix.

Abstract Background SARS-CoV-2 infection is associated with a significant risk of hospitalisation, death, and prolonged impact on quality of life. Evaluation of new treatment options and optimising therapeutic management of people hospitalised with SARS-CoV-2 infection remains essential, but rapid changes in pandemic conditions and potential therapies have limited the utility of traditional approaches to randomised controlled trials. Methods ASCOT ADAPT is an international, investigator-initiated, adaptive platform, randomised controlled trial of therapeutics for non-critically ill patients...

Abstract Background A subset of meningiomas progress in histopathological grade but drivers of progression are poorly understood. We aimed to identify somatic mutations and copy number alterations (CNAs) associated with grade progression in a unique matched tumour dataset. Methods Utilising a prospective database, we identified 10 patients with meningiomas that had undergone grade progression and for whom matched pre- and post-progression tissue (n = 50 samples) was available for targeted next-generation sequencing. Results Mutations in...

Additional file 3. Final Participant Information Sheet Approved by the Ethics Committee Reviewing the SNAP Trial. consent form removed.

Additional file 2. Table S2. Lists of six lncRNAs and 1623 miRNAs (in the attachment of supporting information).

Additional file 3. Table S3. List of filtered 259 lncRNA-miRNA relationship pairs (in the attachment of supporting information).

Abstract Background Investment in a clinical research culture appears to be associated with benefits for consumers, staff, and overall organisational performance. The validated 55-item Research Capacity and Culture (RCC) tool was developed specifically to gauge the research capacity and culture of health professionals and workplace settings within which they work. Results of some individual studies suggest that professional discipline and workplace setting may impact RCC results however it has never been used in a dedicated...

Additional file 1: Table S1. Table displaying optimal cut-offs for the six tumor features determined previously (Walker et al. 2023) in the additive feature combination approach. Table S2. SLS tumors (n=13) that showed discordant MMR IHC findings between clinical diagnostic testing before study entry and testing completed internally during this study and the change in their MMR status and/or pattern of MMR protein loss. Table S3. The concordance between the final MMR IHC result and...

Abstract Routine screening of tumors for DNA mismatch repair (MMR) deficiency (dMMR) in colorectal (CRC), endometrial (EC) and sebaceous skin (SST) tumors leads to a significant proportion of unresolved cases classified as suspected Lynch syndrome (SLS). SLS cases (n = 135) were recruited from Family Cancer Clinics across Australia and New Zealand. Targeted panel sequencing was performed on tumor (n = 137; 80×CRCs, 33×ECs and 24xSSTs) and matched blood-derived DNA to assess for microsatellite instability...

Supplementary Material 7

Abstract Background We aimed to investigate the effects of awake prone positioning (APP) in nonintubated adult patients with acute hypoxemic respiratory failure due to COVID-19. Methods The PubMed, Embase, Web of Science and Cochrane Central Register databases were searched up to June 1, 2022. All randomized trials investigating the effects of APP were included in the present meta-analysis. The primary outcome was intubation rate, and the secondary outcomes included the length of intensive care unit...

Bidirectional fermentation is a technology that utilizes fungi to ferment medicinal edible substrates, with synergistic and complementary advantages. In this work, a fermentation strategy was established to produce a high yield of γ-aminobutyric acid (GABA) and Monascus pigments (MPs) using Monascus and mulberry leaves (MLs). Firstly, the basic fermentation parameters were determined using single-factor experiments, followed by Plackett-Burman (PB) experimental design to identify MLs, glucose, peptone, and temperature as significant influencing factors. The fermentation parameters...

Abstract Background Sodium glucose co-transporter-2 (SGLT2) inhibitors improve long-term cardiovascular and renal outcomes in individuals with type 2 diabetes. However, the safety of SGLT2 inhibitors in ICU patients with type 2 diabetes is uncertain. We aimed to perform a pilot study to assess the relationship between empagliflozin therapy and biochemical, and clinical outcomes in such patients. Methods We included 18 ICU patients with type 2 diabetes receiving empagliflozin (10 mg daily) and insulin to target...

Additional file 2: Supplementary Figure 1. (A) Structural variants plot generated from WGS data of uLMS227 primary patient sample showing intra-chromosomal rearrangements. (B) Copy number profile generated from the uLMS227 patient sample. Total copy number is shown in black and minor copy number in red. Supplementary Figure 2. Methylation analysis of the BRCA1 and RAD51C promoters. Red lines indicate a 100% methylated control, blue lines indicated 0% methylated control and the green line represents the...

Additional file 1: Fig. S1. Kaplan-Meier plots of 28-day survival in patients randomized at low-norepinephrine equivalent dose and high-norepinephrine equivalent dose separated by treatment arms. Fig. S2. Day 28 survival by baseline norepinephrine equivalent dose in all enrolled patients. Fig. S3. Day 28 survival by baseline norepinephrine equivalent dose separated by treatment group. Blue: angiotensin II, red: placebo.

Additional file 1: Fig. S1. Study design and patient eligibility criteria. Narrative S1. Patient death.

Abstract Background Hereditary angioedema (HAE) is a rare disease characterized by unpredictable, recurring subcutaneous or submucosal swelling. Without effective therapy, HAE can negatively impact patients’ quality of life. Management of HAE includes on-demand treatment of attacks and short- and long-term prophylaxis (LTP) to prevent attacks. Newer therapies may be more tolerable and effective in managing HAE; however, therapies such as androgens are still widely used in some countries owing to their relative ease of access...

Demographics, comorbidities, intraoperative and postoperative data for 120 particpants from the SNaPP Pilot Study. Male and female participants aged 40 years and over and presenting for abdominal or thoracic surgery under relaxant general anaesthesia. Assessed at hospital discharge, or after 7 days if still in hospital, for the primary outcome (postoperative pulmonary complications); at 30 days for days alive and at home and at 3 months for quality of life.

Supplementary Material 2 Illumina TSO500 NGS Gene List

Additional file 1. ASCOT ADAPT Statistical Analysis Appendix.

Additional file 1: Table S1. Different multivariate Cox proportional hazards regression models; Table S2. Percentage of missing data in the variables of interest at baseline; Table S3. The impact of Driving pressure and respiratory rate on 28-day mortality in Model D; Table S4. Association between time-varying ventilatory variables and mortality in ARDS patients with different lung morphology after excluding patients receiving ECMO (n=352); Fig. S1. Lung morphology of ARDS patients based on lung CT: non-focal...