30 Works

Mapping the global potential transmission hotspots for severe fever with thrombocytopenia syndrome by machine learning methods

Dong Miao, Ke Dai, Guo-Ping Zhao, Xin-Lou Li, Wen-Qiang Shi, Jiu Song Zhang, Yang Yang, Wei Liu & Li-Qun Fang
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with increasing spread. Currently SFTS transmission has expanded beyond Asian countries, however, with definitive global extents and risk patterns remained obscure. Here we established an exhaustive database that included globally reported locations of human SFTS cases and the competent vector, Haemaphysalis longicornis (H. longicornis), as well as the explanatory environmental variables, based on which, the potential geographic range of H. longicornis and risk areas...

An infectious clone of enterovirus 71(EV71) that is capable of infecting neonatal immune competent mice without adaptive mutations

Huiying Zhang, Zhigang Song, Jingyi Zou, Yanling Feng, Jing Zhang, Lehao Ren, Xiaonan Zhang, Yunwen Hu, Zhenghong Yuan & Zhigang Yi
Enterovirus 71 (EV71) is a major pathogen that causes hand, foot and mouth disease (HFMD), which is a life threatening disease in certain children. The pathogenesis of EV71-caused HFMD is poorly defined due to the lack of simple and robust animal models with severe phenotypes that recapitulate symptoms observed in humans. Here, we generated the infectious clone of a clinical isolate from a severe HFMD patient. Virus rescued from the cDNA clone was infectious in...

Prolonged shedding of severe acute respiratory syndrome coronavirus 2 in patients with COVID-19

Qian Li, Xiao-Shuang Zheng, Xu-Rui Shen, Hao-Rui Si, Xi Wang, Qi Wang, Bei Li, Wei Zhang, Yan Zhu, Ren-Di Jiang, Kai Zhao, Hui Wang, Zheng-Li Shi, Hui-Lan Zhang, Rong-Hui Du & Peng Zhou
Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

Prolonged shedding of severe acute respiratory syndrome coronavirus 2 in patients with COVID-19

Qian Li, Xiao-Shuang Zheng, Xu-Rui Shen, Hao-Rui Si, Xi Wang, Qi Wang, Bei Li, Wei Zhang, Yan Zhu, Ren-Di Jiang, Kai Zhao, Hui Wang, Zheng-Li Shi, Hui-Lan Zhang, Rong-Hui Du & Peng Zhou
Following acute infection, individuals COVID-19 may still shed SARS-CoV-2 RNA. However, limited information is available regarding the active shedding period or whether infectious virus is also shed. Here, we monitored the clinical characteristics and virological features of 38 patients with COVID-19 (long-term carriers) who recovered from the acute disease, but still shed viral RNA for over 3 months. The median carrying history of the long-term carriers was 92 days after the first admission, and the...

Mapping the global potential transmission hotspots for severe fever with thrombocytopenia syndrome by machine learning methods

Dong Miao, Ke Dai, Guo-Ping Zhao, Xin-Lou Li, Wen-Qiang Shi, Jiu Song Zhang, Yang Yang, Wei Liu & Li-Qun Fang
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with increasing spread. Currently SFTS transmission has expanded beyond Asian countries, however, with definitive global extents and risk patterns remained obscure. Here we established an exhaustive database that included globally reported locations of human SFTS cases and the competent vector, Haemaphysalis longicornis (H. longicornis), as well as the explanatory environmental variables, based on which, the potential geographic range of H. longicornis and risk areas...

Long non-coding RNA PCAT1 drives clear cell renal cell carcinoma by upregulating YAP via sponging miR-656 and miR-539

Rui Wang, Bin Zheng, Hongyan Liu & Xiuxian Wan
Clear cell renal cell carcinoma (ccRCC) is the most common RCC subtype with high metastasis, poor prognosis and conventional chemotherapy resistance. Prostate cancer associated transcript 1 (PCAT1) is an important lncRNA that was reported to be involved in cell proliferation, migration and invasion of several types of cancer cells. However, its role in ccRCC is still undetermined. This study found that PCAT1 levels were elevated in ccRCC tumors as well as several ccRCC cells, and...

Increasing SARS-CoV-2 nucleic acid testing capacity during the COVID-19 epidemic in Beijing: experience from a general hospital

Qi Wang, Xiaotao Zhao, Zhihong Yue, Xiaohui Bai, Huiying Rao, Na Han, Tianbing Wang, Hui Wang & Baoguo Jiang
Under the ongoing COVID-19 prevention and control measures in China, increasing the laboratory severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid testing capacity has become the top priority. Since the COVID-19 outbreak in Xinfadi market in Beijing in June 2020, large-scale screening of key populations has been carried out, challenging the nucleic acid testing capabilities of hospital laboratories. Therefore, within 48 hours, Peking University People’s Hospital (PKUPH) transformed a non-nucleic acid testing laboratory into...

β-Sitosterol-loaded solid lipid nanoparticles ameliorate complete Freund’s adjuvant-induced arthritis in rats: involvement of NF-кB and HO-1/Nrf-2 pathway

Feng Zhang, Zhiyu Liu, Xijing He, Zhanqi Li, Bin Shi & Fengmei Cai
Rheumatoid arthritis (RA), autoimmune disease that is categorized via chronic inflammation manifestation, obesity, cardiovascular risk and even enhanced the mortality and affect the 0.3 and 1% of population worldwide. The current experimental study was scrutinize the anti-arthritic effect of β-sitosterol loaded solid lipid nanoparticles (SLN) against complete Fruend adjuvant (CFA)-induced arthritis via dual pathway. Double emulsion solvent displacement method was used for the preparation of β-sitosterol solid lipid nanoparticles (SLN). CFA was used to induce...

BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains

Xiaoli Wu, Yanrong Zheng, Mengru Liu, Yue Li, Shijia Ma, Weidong Tang, Wenping Yan, Ming Cao, Wanqing Zheng, Lei Jiang, Jiaying Wu, Feng Han, Zhenghong Qin, Liang Fang, Weiwei Hu, Zhong Chen & Xiangnan Zhang
Mitophagy, the elimination of damaged mitochondria through autophagy, promotes neuronal survival in cerebral ischemia. Previous studies found deficient mitophagy in ischemic neurons, but the mechanisms are still largely unknown. We determined that BNIP3L/NIX, a mitophagy receptor, was degraded by proteasomes, which led to mitophagy deficiency in both ischemic neurons and brains. BNIP3L exists as a monomer and homodimer in mammalian cells, but the effects of homodimer and monomer on mitophagy are unclear. Site-specific mutations in...

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Chupeng Hu, Wei Liu, Ningyin Xu, An Huang, Zhenxing Zhang, Menghui Fan, Guojing Ruan, Yue Wang, Tao Xi & Yingying Xing
Tissue-resident memory T (TRM) cells, located in the epithelium of most peripheral tissues, constitute the first-line defense against pathogen infections. Our previous study reported that gastric subserous layer (GSL) vaccination induced a “pool” of protective tissue-resident memory CD4+T (CD4+TRM) cells in the gastric epithelium. However, the mechanistic details how CD4+TRM cells form in the gastric epithelium are unknown. Here, our results suggested that the vaccine containing CCF in combination with Silk fibroin hydrogel (SF) broadened...

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Chupeng Hu, Wei Liu, Ningyin Xu, An Huang, Zhenxing Zhang, Menghui Fan, Guojing Ruan, Yue Wang, Tao Xi & Yingying Xing
Tissue-resident memory T (TRM) cells, located in the epithelium of most peripheral tissues, constitute the first-line defense against pathogen infections. Our previous study reported that gastric subserous layer (GSL) vaccination induced a “pool” of protective tissue-resident memory CD4+T (CD4+TRM) cells in the gastric epithelium. However, the mechanistic details how CD4+TRM cells form in the gastric epithelium are unknown. Here, our results suggested that the vaccine containing CCF in combination with Silk fibroin hydrogel (SF) broadened...

Risk factors associated with COVID-19 infection: a retrospective cohort study based on contacts tracing

Tao Liu, Wenjia Liang, Haojie Zhong, Jianfeng He, Zihui Chen, Guanhao He, Tie Song, Shaowei Chen, Ping Wang, Jialing Li, Yunhua Lan, Mingji Cheng, Jinxu Huang, Jiwei Niu, Liang Xia, Jianpeng Xiao, Jianxiong Hu, Lifeng Lin, Qiong Huang, Zuhua Rong, Aiping Deng, Weilin Zeng, Jiansen Li, Xing Li, Xiaohua Tan … & Wenjun Ma
This study aimed to estimate the attack rates, and identify the risk factors of COVID-19 infection. Based on a retrospective cohort study, we investigated 11,580 contacts of COVID-19 cases in Guangdong Province from 10 January to 15 March 2020. All contacts were tested by RT-PCR to detect their infection of SARS-COV-2. Attack rates by characteristics were calculated. Logistic regression was used to estimate the risk factors of infection for COVID-19. A total of 515 of...

Convergence of carbapenem resistance and hypervirulence in a highly-transmissible ST11 clone of K. pneumoniae: An epidemiological, genomic and functional study

Ping Li, Qiqiang Liang, Wugao Liu, Beiwen Zheng, Lizhang Liu, Wei Wang, Zhijiang Xu, Man Huang & Youjun Feng
Co-occurrence of hypervirulence and KPC-2 carbapenem resistant phenotypes in a highly-transmissible ST11 clone ofKlebsiella pneumoniae has elicited deep concerns from public health stand point. To address this puzzle, we conducted a large-scale epidemiological, clinical and genomic study of K. pneumonia ST11 clones with both hypervirulence and carbapenem resistance in two tertiary hospitals in Zhejiang province. Most of the patients (15/23) were diagnosed with exclusively carbapenem-resistant K. pneumoniae (CRKP) infections. Ten death cases were reported, some...

Burkholderia pseudomallei interferes with host lipid metabolism via NR1D2-mediated PNPLA2/ATGL suppression to block autophagy-dependent inhibition of infection

Mengling Tang, Zhiqiang Hu, Chenglong Rao, Jiangao Chen, Siqi Yuan, Jiangang Zhang, Chan Mao, Jingmin Yan, Yupei Xia, Meijuan Zhang, Juanjuan Yue, Yang Xiang, Jianping Xie, Xuhu Mao & Qian Li
Burkholderia pseudomallei: which causes melioidosis with high mortality in humans, has become a global public health concern. Recently, infection-driven lipid droplet accumulation has been related to the progression of host-pathogen interactions, and its contribution to the pathogenesis of infectious disease has been investigated. Here, we demonstrated that B. pseudomallei infection actively induced a time-dependent increase in the number and size of lipid droplets in human lung epithelial cells and macrophages. We also found that lipid...

Synergistic ultrasonic biophysical effect-responsive nanoparticles for enhanced gene delivery to ovarian cancer stem cells

Chun Liufu, Yue Li, Yan Lin, Jinsui Yu, Meng Du, Yuhao Chen, Yaozhang Yang, Xiaojing Gong & Zhiyi Chen
Ovarian cancer stem cells (OCSCs) that are a subpopulation within bulk tumor survive chemotherapy and conduce to chemo-resistance and tumor relapse. However, conventional gene delivery is unsuitable for the on-demand content release, which limits OCSCs therapeutic utility. Here, we reported ultrasound-targeted microbubble destruction (UTMD)-triggerable poly(ethylene glycol)-disulfide bond-polyethylenimine loaded microbubble (PSP@MB). Taking advantage of glutathione (GSH) responsiveness, ultrasound triggering and spatiotemporally controlled release manner, PSP@MB is expected to realize local gene delivery for OCSCs treatment. But...

Synergistic ultrasonic biophysical effect-responsive nanoparticles for enhanced gene delivery to ovarian cancer stem cells

Chun Liufu, Yue Li, Yan Lin, Jinsui Yu, Meng Du, Yuhao Chen, Yaozhang Yang, Xiaojing Gong & Zhiyi Chen
Ovarian cancer stem cells (OCSCs) that are a subpopulation within bulk tumor survive chemotherapy and conduce to chemo-resistance and tumor relapse. However, conventional gene delivery is unsuitable for the on-demand content release, which limits OCSCs therapeutic utility. Here, we reported ultrasound-targeted microbubble destruction (UTMD)-triggerable poly(ethylene glycol)-disulfide bond-polyethylenimine loaded microbubble (PSP@MB). Taking advantage of glutathione (GSH) responsiveness, ultrasound triggering and spatiotemporally controlled release manner, PSP@MB is expected to realize local gene delivery for OCSCs treatment. But...

Profiling serum cytokines in COVID-19 patients reveals IL-6 and IL-10 are disease severity predictors

Huan Han, Qingfeng Ma, Cong Li, Rui Liu, Li Zhao, Wei Wang, Pingan Zhang, Xinghui Liu, Guosheng Gao, Fang Liu, Yingan Jiang, Xiaoming Cheng, Chengliang Zhu & Yuchen Xia
Since the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China, it has rapidly spread across many other countries. While the majority of patients were considered mild, critically ill patients involving respiratory failure and multiple organ dysfunction syndrome are not uncommon, which could result death. We hypothesized that cytokine storm is associated with severe outcome. We enrolled 102 COVID-19 patients who were admitted to Renmin Hospital (Wuhan, China). All patients were classified into moderate, severe...

Burkholderia pseudomallei interferes with host lipid metabolism via NR1D2-mediated PNPLA2/ATGL suppression to block autophagy-dependent inhibition of infection

Mengling Tang, Zhiqiang Hu, Chenglong Rao, Jiangao Chen, Siqi Yuan, Jiangang Zhang, Chan Mao, Jingmin Yan, Yupei Xia, Meijuan Zhang, Juanjuan Yue, Yang Xiang, Jianping Xie, Xuhu Mao & Qian Li
Burkholderia pseudomallei: which causes melioidosis with high mortality in humans, has become a global public health concern. Recently, infection-driven lipid droplet accumulation has been related to the progression of host-pathogen interactions, and its contribution to the pathogenesis of infectious disease has been investigated. Here, we demonstrated that B. pseudomallei infection actively induced a time-dependent increase in the number and size of lipid droplets in human lung epithelial cells and macrophages. We also found that lipid...

Convergence of carbapenem resistance and hypervirulence in a highly-transmissible ST11 clone of K. pneumoniae: An epidemiological, genomic and functional study

Ping Li, Qiqiang Liang, Wugao Liu, Beiwen Zheng, Lizhang Liu, Wei Wang, Zhijiang Xu, Man Huang & Youjun Feng
Co-occurrence of hypervirulence and KPC-2 carbapenem resistant phenotypes in a highly-transmissible ST11 clone ofKlebsiella pneumoniae has elicited deep concerns from public health stand point. To address this puzzle, we conducted a large-scale epidemiological, clinical and genomic study of K. pneumonia ST11 clones with both hypervirulence and carbapenem resistance in two tertiary hospitals in Zhejiang province. Most of the patients (15/23) were diagnosed with exclusively carbapenem-resistant K. pneumoniae (CRKP) infections. Ten death cases were reported, some...

BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains

Xiaoli Wu, Yanrong Zheng, Mengru Liu, Yue Li, Shijia Ma, Weidong Tang, Wenping Yan, Ming Cao, Wanqing Zheng, Lei Jiang, Jiaying Wu, Feng Han, Zhenghong Qin, Liang Fang, Weiwei Hu, Zhong Chen & Xiangnan Zhang
Mitophagy, the elimination of damaged mitochondria through autophagy, promotes neuronal survival in cerebral ischemia. Previous studies found deficient mitophagy in ischemic neurons, but the mechanisms are still largely unknown. We determined that BNIP3L/NIX, a mitophagy receptor, was degraded by proteasomes, which led to mitophagy deficiency in both ischemic neurons and brains. BNIP3L exists as a monomer and homodimer in mammalian cells, but the effects of homodimer and monomer on mitophagy are unclear. Site-specific mutations in...

β-Sitosterol-loaded solid lipid nanoparticles ameliorate complete Freund’s adjuvant-induced arthritis in rats: involvement of NF-кB and HO-1/Nrf-2 pathway

Feng Zhang, Zhiyu Liu, Xijing He, Zhanqi Li, Bin Shi & Fengmei Cai
Rheumatoid arthritis (RA), autoimmune disease that is categorized via chronic inflammation manifestation, obesity, cardiovascular risk and even enhanced the mortality and affect the 0.3 and 1% of population worldwide. The current experimental study was scrutinize the anti-arthritic effect of β-sitosterol loaded solid lipid nanoparticles (SLN) against complete Fruend adjuvant (CFA)-induced arthritis via dual pathway. Double emulsion solvent displacement method was used for the preparation of β-sitosterol solid lipid nanoparticles (SLN). CFA was used to induce...

Silk fibroin hydrogel as mucosal vaccine carrier: induction of gastric CD4+TRM cells mediated by inflammatory response induces optimal immune protection against Helicobacter felis

Chupeng Hu, Wei Liu, Ningyin Xu, An Huang, Zhenxing Zhang, Menghui Fan, Guojing Ruan, Yue Wang, Tao Xi & Yingying Xing
Tissue-resident memory T (TRM) cells, located in the epithelium of most peripheral tissues, constitute the first-line defense against pathogen infections. Our previous study reported that gastric subserous layer (GSL) vaccination induced a “pool” of protective tissue-resident memory CD4+T (CD4+TRM) cells in the gastric epithelium. However, the mechanistic details how CD4+TRM cells form in the gastric epithelium are unknown. Here, our results suggested that the vaccine containing CCF in combination with Silk fibroin hydrogel (SF) broadened...

Risk factors associated with COVID-19 infection: a retrospective cohort study based on contacts tracing

Tao Liu, Wenjia Liang, Haojie Zhong, Jianfeng He, Zihui Chen, Guanhao He, Tie Song, Shaowei Chen, Ping Wang, Jialing Li, Yunhua Lan, Mingji Cheng, Jinxu Huang, Jiwei Niu, Liang Xia, Jianpeng Xiao, Jianxiong Hu, Lifeng Lin, Qiong Huang, Zuhua Rong, Aiping Deng, Weilin Zeng, Jiansen Li, Xing Li, Xiaohua Tan … & Wenjun Ma
Objectives To estimate the attack rates, and identify the risk factors of COVID-19 infection. Methods Based on a retrospective cohort study, we investigated 11,580 contacts of COVID-19 cases in Guangdong Province from January 10 to March 15, 2020. All contacts were tested by RT-PCR to detect their infection of SARS-COV-2. Attack rates by characteristics were calculated, and logistic regression was used to estimate the risk factors of infection for COVID-19. Results A total of 515...

Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniae

Shijun Sun, Hua Gao, Yudong Liu, Longyang Jin, Ruobing Wang, Xiaojuan Wang, Qi Wang, Yuyao Yin, Yawei Zhang & Hui Wang
Tigecycline is considered one of the last-resort antimicrobials for carbapenem-resistant K. pneumoniae. Plasmid-mediated tigecycline resistance remains largely unclear. Here, by utilizing whole genome sequencing, we report a plasmid-mediated tigecycline resistance mechanism, a 6,489 bp Resistance-nodulation-division family (RND) efflux pump (tmexCD1-toprJ1 pump), that confers transferable tigecycline resistance in K pneumoniae isolated from patients and chickens. In addition, we identified high prevalence of the plasmids co-harbouring both tmexCD1-toprJ1 pump and mcr (tmexCD1-mcr co-harbouring plasmid) from human in...

Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene mcr in one plasmid confers transferable multidrug resistance in Klebsiella pneumoniae

Shijun Sun, Hua Gao, Yudong Liu, Longyang Jin, Ruobing Wang, Xiaojuan Wang, Qi Wang, Yuyao Yin, Yawei Zhang & Hui Wang
Tigecycline is considered one of the last-resort antimicrobials for carbapenem-resistant K. pneumoniae. Plasmid-mediated tigecycline resistance remains largely unclear. Here, by utilizing whole genome sequencing, we report a plasmid-mediated tigecycline resistance mechanism, a 6,489 bp Resistance-nodulation-division family (RND) efflux pump (tmexCD1-toprJ1 pump), that confers transferable tigecycline resistance in K pneumoniae isolated from patients and chickens. In addition, we identified high prevalence of the plasmids co-harbouring both tmexCD1-toprJ1 pump and mcr (tmexCD1-mcr co-harbouring plasmid) from human in...

Registration Year

  • 2020
    30

Resource Types

  • Text
    16
  • Dataset
    12
  • Other
    2

Affiliations

  • Sixth Affiliated Hospital of Sun Yat-sen University
    30
  • Fudan University
    23
  • Sun Yat-sen University
    21
  • Zhejiang University
    19
  • Shandong University
    19
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    19
  • Huazhong University of Science and Technology
    17
  • Capital Medical University
    17
  • Tongji University
    17
  • Central South University
    16