Additional file 2 of Sphingosine kinase 1 contributes to the metastatic potential of epithelial ovarian cancer to the adipocyte-rich nicheChen Wang, Taiyang Ye, Wenjing Wang, Keqi Song, Jie Zhu, Lan Dai & Wen Di
Additional file 2: Fig. S1 Inhibition of SphK1 by siRNA suppressed adipocyte-induced metastasis of HEY cells. A Adipocyte isolation. Primary human omentum tissues were collected and mature adipocytes were extracted. Adipocytes were visualized by (i) microscopy, (ii) stained with Oil red O to confirm the extraction of mature adipocytes, and (iii) by fluorescence microscopy with calcein-AM to confirm viability. The scale bar represents 100 μm. B HEY cells were transfected with negative control siRNA or...
Additional file 3 of Sphingosine kinase 1 contributes to the metastatic potential of epithelial ovarian cancer to the adipocyte-rich nicheChen Wang, Taiyang Ye, Wenjing Wang, Keqi Song, Jie Zhu, Lan Dai & Wen Di
Additional file 3: Fig. S2 SphK1 inhibitor PF543 suppressed adipocyte-induced metastasis of EOC cells. A Serum-starved SKOV3 and HEY cells were treated with increasing doses of PF543 as indicated for 2 h. Expression level of SphK1 was determined by Western blot and normalized to GAPDH. Densitometric analysis of SphK1 was shown on the right. Data are mean ± SD (n = 3). ns, no significance versus control; two-tailed Student’s t test. B Serum-starved SKOV3 and...
Isolation of Diaphorobacter sp. LW2 capable of degrading Phenanthrene and its migration mediated by DiaphorobacterJialu Li, Mei Hong, Rui Tang, Tingchen Cui, Yadong Yang, Jing Lv, Na Liu & Yutao Lei
Phenanthrene, one of the polycyclic aromatic hydrocarbons, is stubborn and persistent and exists widely in petroleum-contaminated soil. Filamentous fungi are good assistants to bacterial transport, by hyphae passing through soil pores and reaching further positions. An isolated bacterial strain, from the contaminated soil of the coking plant, was identified as Diaphorobacter and named LW2, which could use phenanthrene as the only carbon source and energy for its growth. LW2 could degrade phenanthrene in a wide...
Biomarkers for isolated congenital heart disease based on maternal amniotic fluid metabolomics analysisXuelian Yuan, Lu Li, Hong Kang, Meixian Wang, Jing Zeng, Yanfang Lei, Nana Li, Ping Yu, Xiaohong Li & Zhen Liu
Abstract Introduction Congenital heart disease (CHD) is one of the most prevalent birth defects in the world. The pathogenesis of CHD is complex and unclear. With the development of metabolomics technology, variations in metabolites may provide new clues about the causes of CHD and may serve as a biomarker during pregnancy. Methods Sixty-five amniotic fluid samples (28 cases and 37 controls) during the second and third trimesters were utilized in this study. The metabolomics of...
Fibrinogen and antithrombin III are associated with in-hospital mortality among critically ill patients with acute kidney injuryGuangyuan Zhang, Lei Zhang, Sun Si, Tiancheng Jiang, Yi Xia, Yongkun Zhu, Xiangyu Zhang, Chi Yao, Ming Chen & Shuqiu Chen
Coagulation factors participates in the inflammatory cascade, known to play a crucial role in the development of acute kidney injury (AKI). Thus, it’s likely that some factors may be associated with AKI. Among them, low levels of fibrinogen and antithrombin III (ATIII) activity have been proved to increase mortality in patients with sepsis. Moreover, they are also reported to be associated with higher incidence of AKI. However, the association between coagulation parameters, especially fibrinogen and...
The establishment of structure activity relationship (SAR) for rakicidin derivatives is pretty vital to develop rakicidins as a new type of anti-cancer agents. Herein, two novel rakicidin derivatives, compounds B1-1 (1) and B1-2 (2), a cyclic depsipeptide and a chain lipopeptide, respectively, were isolated from culture broth of Micromonospora chalcea FIM-R160609, and their structures were elucidated clearly by extensive NMR and HR-ESI-MS analyses. Following, their cytotoxic activities were evaluated against HCT-8 and PANC-1 human cancer...
sj-tif-3-onc-10.1177_11795549221134735 – Supplemental material for First-Line Osimertinib in Patients With EGFR-Mutated Non-Small Cell Lung Cancer: Effectiveness, Resistance Mechanisms, and Prognosis of Different Subsequent TreatmentsNaifu Nie, Jianghua Li, Jian Zhang, Jie Dai, Zhulin Liu, Zhenyu Ding, Yubo Wang, Mengxiao Zhu, Chen Hu, Rui Han, Huan Tang, Li Li & Yong He
Supplemental material, sj-tif-3-onc-10.1177_11795549221134735 for First-Line Osimertinib in Patients With EGFR-Mutated Non-Small Cell Lung Cancer: Effectiveness, Resistance Mechanisms, and Prognosis of Different Subsequent Treatments by Naifu Nie, Jianghua Li, Jian Zhang, Jie Dai, Zhulin Liu, Zhenyu Ding, Yubo Wang, Mengxiao Zhu, Chen Hu, Rui Han, Huan Tang, Li Li and Yong He in Clinical Medicine Insights: Oncology
First-Line Osimertinib in Patients With EGFR-Mutated Non-Small Cell Lung Cancer: Effectiveness, Resistance Mechanisms, and Prognosis of Different Subsequent TreatmentsNaifu Nie, Jianghua Li, Jian Zhang, Jie Dai, Zhulin Liu, Zhenyu Ding, Yubo Wang, Mengxiao Zhu, Chen Hu, Rui Han, Huan Tang, Li Li & Yong He
Background:Although the clinical application of osimertinib, a third-generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), has been a new step forward in the first-line treatment of non-small cell lung cancer (NSCLC), an increasing number of patients with progression on osimertinib represents a great challenge clinically. The patterns of resistance mechanisms and subsequent treatment strategies after first-line osimertinib resistance are not well established.Methods:Between January 1, 2016 and October 31, 2020, a consecutive of 56 EGFR-mutant lung...
Additional file 1 of Association of education background with clinical pharmacists’ clinical pharmacy workload in tertiary hospitals of ChinaQingran Sun, Lei Chen, Yuankai Huang & Xiaoyu Xi
Additional file 1: Appendix 1. Questionnaire for education background and workload of clinical pharmacists (English version).
METTL3-mediated long non-coding RNA MIR99AHG methylation targets miR-4660 to promote bone marrow mesenchymal stem cell osteogenic differentiationLintao Li, Beiyue Wang, Xing Zhou, Hao Ding, Chang Sun, Yicun Wang, Fan Zhang & Jianning Zhao
Whether long non-coding RNA Mir-99a-Let-7c Cluster Host Gene (LncRNA MIR99AHG) is involved in osteoporosis (OP) remains vague, so we hereby center on its implication. Old C57BL/6J mice were injected with the silencing lentivirus of MIR99AHG and subjected to microCT analysis and immunohistochemistry on osteogenic cells. The osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) with or without transfection was determined by alkaline phosphatase (ALP) and Alizarin Red S staining. Total N(6)-methyladenosine (m6A) on the...
Additional file 2 of AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitinationRou Zhao, Baoyu He, Qingli Bie, Jinghe Cao, Haoran Lu, Zhixin Zhang, Jing Liang, Li Wei, Huabao Xiong & Bin Zhang
Additional file 2: Supplementary Table 1. Sequences ofreal-time PCR primers. Supplementary Table 2. Primary Antibodies. Supplementary Table 3. Second Antibodies. Supplementary Table 4. ShRNA or siRNA Oligonucleotides. Supplementary Table 5. Tumorigenicityof knockdown AQP5 and control group. Supplementary Table 6. Tumorigenicity of knockdown AQP5 and control group. Supplementary Table 7. Tumorigenicityof knockdown AQP5 and LGR5. Supplementary Table 8. Differentially expressed genes between exogenousoverexpression of AQP5 and control group. Supplementary Table 9. Differentially expressed genes between exogenousoverexpression...
Additional file 3 of AQP5 complements LGR5 to determine the fates of gastric cancer stem cells through regulating ULK1 ubiquitinationRou Zhao, Baoyu He, Qingli Bie, Jinghe Cao, Haoran Lu, Zhixin Zhang, Jing Liang, Li Wei, Huabao Xiong & Bin Zhang
Additional file 3.
Additional file 1 of TSH levels within the normal range and risk of cardiovascular and all-cause mortality among individuals with diabetesPing Zhu, Guojuan Lao, Chuping Chen, Lihui Luo, Jing Gu & Jianmin Ran
Additional file 1: Figure S1. Weighted Kaplan–Meier survival curves for all-cause (A) and CVD (B) mortality based on tertiles of TSH levels among patients with diabetes. The low-normal TSH (tertile 1) indicates serum TSH levels of 0.39 to 1.30 mIU/L; medium-normal TSH (tertile 2), serum TSH levels of 1.30 to 2.09 mIU/L; and high-normal TSH (tertile 3), serum TSH levels of 2.09 to 4.60 mIU/L.
Additional file 1 of UBR7 inhibits HCC tumorigenesis by targeting Keap1/Nrf2/Bach1/HK2 and glycolysisLiang Zhao, Min Kang, Xiaomeng Liu, Zhenran Wang, Yan Wang, Haiqiang Chen, Wenhui Liu, Shiqian Liu, Baibei Li, Chong Li, Antao Chang & Bo Tang
Additional file 1.
Additional file 1 of Optimized dose selective HDAC inhibitor tucidinostat overcomes anti-PD-L1 antibody resistance in experimental solid tumorsPei Zhang, Yang Du, Hua Bai, Zhijie Wang, Jianchun Duan, Xin Wang, Jia Zhong, Rui Wan, Jiachen Xu, Xiran He, Di Wang, Kailun Fei, Ruofei Yu, Jie Tian & Jie Wang
Additional file 1. The effect of tucidinostat on cell proliferation, apoptosis and hepatonephric function. a Comparison of cell proliferation of 4T1, LLC, and CT26 cells treated with different doses (2.5, 5, 7.5 μM) of tucidinostat for 24 h by CCK-8 assay. b Comparison of cell apoptosis of 4T1, LLC, and CT26 cells treated with different doses of tucidinostat (2.5, 5, 7.5 μM) for 6 h by Annexin V-FITC/PI assay. c Mouse CT26 cells (5 ×...
Additional file 6 of Inhibition of platelet activation suppresses reactive enteric glia and mitigates intestinal barrier dysfunction during sepsisBo Cheng, Mengyu Du, Shuxuan He, Lan Yang, Xi Wang, Hui Gao, Haiqing Chang, Wei Gao, Yan Li, Qiang Wang & Yansong Li
Additional file 6: Figure S6. Single-cell sequencing analysis of adult mice intestine. a Dotplot for highest specificity gene markers of cell clusters. Dot size represents the percentage of cells expressing the denoted gene, and the color represents average normalized expression level within the denoted cluster. b The expression of GFAP in different cell clusters by analysis of the Uniform Manifold Approximation and Projection (UMAP). c Different cell subpopulation of the GSE156905 dataset derived from the...
Additional file 1 of Aligned nanofibrous collagen membranes from fish swim bladder as a tough and acid-resistant suture for pH-regulated stomach perforation and tendon ruptureZhaohui Luan, Shuang Liu, Wei Wang, Kaige Xu, Shaosong Ye, Ruijue Dan, Hong Zhang, Zhenzhen Shu, Tongchuan Wang, Chaoqiang Fan, Malcolm Xing & Shiming Yang
Additional file 1: Supplementary Fig. S1. PGA with highly complex structure under SEM. Supplementary Fig. S2. Two other crosslinking methods. Supplementary Fig. S3. The detailed process of fabricating DCDS suture with standardization. Supplementary Fig. S4. The tensile strength of double-layers swim bladder with and without crosslinking.
Aligned nanofibrous collagen membranes from fish swim bladder as a tough and acid-resistant suture for pH-regulated stomach perforation and tendon ruptureZhaohui Luan, Shuang Liu, Wei Wang, Kaige Xu, Shaosong Ye, Ruijue Dan, Hong Zhang, Zhenzhen Shu, Tongchuan Wang, Chaoqiang Fan, Malcolm Xing & Shiming Yang
Abstract Background Wound closure in the complex body environment places higher requirements on suture’s mechanical and biological performance. In the scenario of frequent mechanical gastric motility and extremely low pH, single functional sutures have limitations in dealing with stomach bleeding trauma where the normal healing will get deteriorated in acid. It necessitates to advance suture, which can regulate wounds, resist acid and intelligently sense stomach pH. Methods Based on fish swim bladder, a double-stranded drug-loaded...
Additional file 2 of Relationship between serum homocysteine, fibrinogen, lipoprotein-a level, and peripheral arterial disease: a dose–response meta-analysisHecheng Wang, Pengpeng Wu, Deying Jiang, Hao Zhang, Jian Zhang, Yu Zong & Yanshuo Han
Additional file 2: Table S2. Characteristics of individual studies on Hcy, FIB, LPa and risk of PADs.
Relationship between serum homocysteine, fibrinogen, lipoprotein-a level, and peripheral arterial disease: a dose–response meta-analysisHecheng Wang, Pengpeng Wu, Deying Jiang, Hao Zhang, Jian Zhang, Yu Zong & Yanshuo Han
Abstract Aim At present, the relationship between serum homocysteine (Hcy), fibrinogen (FIB), lipoprotein-a (LPa), and PAD is uncertain, and there has been no meta-analysis to establish the dose–response relationship between their exposure levels and PAD. Methods and results Relevant literature published in PubMed, Embase, and Web of Science was retrieved. The robust error meta-regression method was used to assess the linear and non-linear dose–response relationship between exposure level and PAD risk. A total of 68...
Additional file 1 of Effects of qigong exercise on the physical and mental health of college students: a systematic review and Meta-analysisJianping Lin, Yi fang Gao, Yue Guo, Ming Li, Yuxiang Zhu, Ruoshi You, Shaoqing Chen & Shizhong Wang
Supplementary Material 1: Figure S1. Sensitivity analysis of the effect of Qigong interventions on depression
Additional file 3 of Effects of qigong exercise on the physical and mental health of college students: a systematic review and Meta-analysisJianping Lin, Yi fang Gao, Yue Guo, Ming Li, Yuxiang Zhu, Ruoshi You, Shaoqing Chen & Shizhong Wang
Supplementary Material 3: PRISMA checklist
Association of education background with clinical pharmacists’ clinical pharmacy workload in tertiary hospitals of ChinaQingran Sun, Lei Chen, Yuankai Huang & Xiaoyu Xi
Abstract Background Workload of healthcare providers may affect the quality of healthcare. Practical evidences have indicated that healthcare providers are differentially associated with workload due to their different education and training background. Clinical pharmacists are an indispensable part of medical teams. Under the precondition that clinical pharmacists in China generally undertake uneven clinical pharmacy workload, the relationship of workload and clinical pharmacists’ different education backgrounds remains unclear. This study aimed to assess the association between...
Additional file 3 of Integrative analyses of maternal plasma cell-free DNA nucleosome footprint differences reveal chromosomal aneuploidy fetuses gene expression profileMin Zhang, Kun Li, Shoufang Qu, Zhiwei Guo, Yuanli Wang, Xu Yang, Junhua Zhou, Guojun Ouyang, Rongtao Weng, Fenxia Li, Yingsong Wu & Xuexi Yang
Additional file 3: Table S3. Differential promoter coverage genes and GO, KEGG enrichment analysis results of differential genes for pregnant women with trisomy 18.
Additional file 6 of Exogenous ABA and IAA modulate physiological and hormonal adaptation strategies in Cleistocalyx operculatus and Syzygium jambos under long-term waterlogging conditionsEl-Hadji Malick Cisse, Juan Zhang, Da-Dong Li, Ling-Feng Miao, Li-Yan Yin & Fan Yang
Additional file 6.
Sun Yat-sen University6,372
Chinese Academy of Medical Sciences & Peking Union Medical College4,155
Shanghai Jiao Tong University3,838
Capital Medical University3,247
Huazhong University of Science and Technology3,118
Chinese Academy of Sciences3,047
University of Chinese Academy of Sciences2,681