Traditional grating lenses can accumulate phase for adjusting wavefronts, and plasmonic resonances can be excited in metasurfaces with discrete structures for optical field modulation. Diffractive and plasma optics have been developing in parallel, with easy processing, small size, and dynamic control advantages. Due to theoretical hybridization, structural design can combine advantages and show great potential value. Changing such as the shape and size of the flat metasurface can easy produce light field reflections, but 3D...

Supplement 1

Additional file 1. Original data of WB.

Additional file 4: Figure S3. Interference of LncRNA-TBP inhibits fatty acid oxidation in skeletal muscle. (A-F) Relative LncRNA-TBP expression (n = 4) (A), relative mtDNA content (n = 4) (B), relative fatty acid β-oxidation rate (n = 4) (C), relative FFA and TG content (n = 4) (D), relative mRNA (n = 6) (E) and protein (n = 3) (F) expression levels of fatty acid oxidation or synthesis related-genes in gastrocnemius with LncRNA-TBP interference in...

Additional file 4: Figure S3. Interference of LncRNA-TBP inhibits fatty acid oxidation in skeletal muscle. (A-F) Relative LncRNA-TBP expression (n = 4) (A), relative mtDNA content (n = 4) (B), relative fatty acid β-oxidation rate (n = 4) (C), relative FFA and TG content (n = 4) (D), relative mRNA (n = 6) (E) and protein (n = 3) (F) expression levels of fatty acid oxidation or synthesis related-genes in gastrocnemius with LncRNA-TBP interference in...

Additional file 5: Figure S4. Interference of LncRNA-TBP activates fast-twitch muscle phenotype and reduces muscle hypertrophy. (A-N) relative enzymes activity of LDH and SDH (n = 4) (A), Relative glycogen content (n = 5) (B), relative mRNA expression levels of glycogenolytic and glycolytic genes (n = 6) (C), relative mRNA expression levels of several fast-/slow-twitch myofiber genes (n = 6) (D), immunohistochemistry analysis of MYH1A/MYH7B (n = 3) (E), MYH1A/MYH7B protein content (n = 8)...

Additional file 9: Figure S8. The expression and location analysis of TBP. (A) Relative TBP expression during the proliferation and differentiation of CPM isolated from XH chicken (n = 4). (B) Subcellular location of TBP protein annotated by UniProt Knowledgebase ( https://www.uniprot.org/ ). In panels (A), results are presented as mean ± SEM.

Additional file 2: Figure S1. Conservative analysis of LncRNA-TBP performed by using the NCBI’s BLAST. A total of eighteen species, including Anas platyrhynchos, Anser cygnoides, Apteryx mantelli mantelli, Aquila chrysaetos, Bos taurus, Coturnix japonica, Gallus gallus, Geospiza fortis, Homo sapiens, Meleagris gallopavo, Melopsittacus undulatus, Mus musculus, Numida meleagris, Ovis aries, Pan troglodytes, Rattus norvegicus, Sus scrofa and Zebra finch were used for Nucleotide BLAST. Top 4 most conservative results were listed above.

Abstract Background Glycyrrhizic acid (GA), a saponin compound often used as a flavoring agent, can elicit anti-inflammatory and anti-tumor effects, and alleviate aging. However, the specific mechanism by which GA alters immune cell populations to produce these beneficial effects is currently unclear. Results In this study, we systematically analyzed single-cell sequencing data of peripheral blood mononuclear cells from young mice, aged mice, and GA-treated aged mice. Our in vivo results show that GA reduced senescence-induced...

Additional file 3: Fig. S3. The overall methylation profile of COL12A1 gene in iCCA and adjacent nontumor tissue samples from TCGA (A) and GSE156299 (B) dataset, respectively. TCGA, The Cancer Genome Atlas; iCCA, intrahepatic cholangiocarcinoma.

Additional file 3: Table S3. The frequency of mutation site in 5000 normal population.

Abstract Background Metastasis is the predominant cause of mortality in patients with breast cancer. Long noncoding RNAs (lncRNAs) have been shown to drive important phenotypes in tumors, including invasion and metastasis. However, the lncRNAs involved in metastasis and their molecular and cellular mechanisms are still largely unknown. Methods The transcriptional and posttranscriptional processing of LINC00478-associated cytoplasmic RNA (LacRNA) was determined by RT-qPCR, semiquantitative PCR and 5′/3′ RACE. Paired-guide CRISPR/cas9 and CRISPR/dead-Cas9 systems was used to...

Additional file 1.

Additional file 3.

Additional file 4.

Additional file 6.

Additional file 2. Additional dataset S1.

Additional file 1: Table S1. All siRNAs/shRNAs used in this research.

Additional file 2: Table S2. All PCR primers used in this research.

Additional file 4: Figure S2. GO and KEGG analysis of circFAM13B relative mRNA sequencing. A. GO analysis was conducted on the differentially expressed genes of mRNA sequencing. B. KEGG analysis was conducted on the differentially expressed genes of mRNA sequencing. C. Western blot was conducted to investigate the influence of circFAM13B on PD-L1 in T24 cells. D. Western blot was conducted to investigate the influence of circFAM13B on PD-L1 in UMUC3 cells.

Additional file 5: Figure S3. PKM2 knockdown inhibited the immune escape of BCa cells. A-B. QRT-PCR assays were performed to validate the efficiency of PKM2 siRNAs transfection in T24 and UMUC3 cells (***P<0.001, Student’s t-test). C–D. ELISA assays were carried out to detect the granzyme B and IFN-γ produced by CD8+ T cells, which were co-cultured with PKM2 siRNAs transfected or control T24 and UMUC3 cells (*P<0.05, **P<0.01, ***P<0.001, Student’s t-test). E–F. The killing ability...

Additional file 6: Figure S4. IGF2BP1 promoted the proliferation of BCa cells. A-B. CCK8 assays were carried out in IGF2BP1 siRNAs transfected or control T24 and UMUC3 cells (***P<0.001, Student’s t-test). C–D. Colony formation assays were performed in IGF2BP1 siRNAs transfected or control T24 and UMUC3 cells (**P<0.01, Student’s t-test). Data are expressed as mean±SD, n=3.

Additional file 7: Figure S5. IGF2BP1 overexpression rescued the repressed proliferation induced by circFAM13B in BCa. A-B. CCK8 assays showed that the overexpression of IGF2BP1 rescued the inhibition of proliferation caused by circFAM13B in T24 and UMUC3 cells (**P<0.01, ***P<0.001, Student’s t-test). C–D. Colony formation assays showed that the overexpression of IGF2BP1 rescued the inhibition of proliferation caused by circFAM13B in T24 and UMUC3 cells (*P<0.05, **P<0.01, Student’s t-test). E. qRT-PCR confirmed circFAM13B inhibited PKM2...

Abstract Lung cancer has one of the highest morbidity and mortality rates in the world. Frailty is common in many countries and is a major cause of premature functional decline and premature death in older adults, and may affect the treatment and prognosis of lung cancer patients. To investigate the predictive value of frailty at diagnosis on all-cause mortality in lung cancer patients, this study retrospectively collected and analysed clinical information on lung cancer patients...

Additional file 8: Figure S6. Construction of HNRNPL knockdown T24 and UMUC3 cells. A. GO analysis was conducted on the proteins pulled down by flanking introns probe. B. The results of RNAct database predictions and circFAM13B flanking introns relative mass spectrometry analysis were intersected, and HNRNPL and ADAR1 were found. C. The expression of ADAR1 and circFAM13B in T24 or UMUC3 cells were confirmed by qRT-PCR after ADAR1 siRNA transfection (**P<0.01, ***P<0.001, Student’s t-test). D....