Whether hemostatic status was correlated with the diverse types of acute kidney injury in cirrhotic patients is unclear. The present study aimed to investigate the relationship between hemostatic markers and the diverse types of acute kidney injury (AKI) in liver cirrhosis. Cirrhotic patients with consecutive treatment at the First Affiliated Hospital of Medicine School, Zhejiang University, were pooled in a cohort. Their demographic and clinical data, biochemistry parameters and hemostatic markers were assessed to identify...

To investigate the effect of IL-27 on Th9 differentiation and Th1/Th2 balance. C57BL/6 (B6) mice were treated with ovalbumin to establish an allergic asthma (AA) model and subjected to IL-27 overexpression (OV) and empty vector (EV). Hematoxylin-eosin (HE) staining was performed to observe lung tissue inflammation. Flow cytometry was carried out to evaluate the percentage of Th9, Th1, and Th2 cells. The expression of IL-27, IL-27R, IL-9, T-bet, IFN-γ, and IgE was evaluated by quantitative...

To investigate the effect of IL-27 on Th9 differentiation and Th1/Th2 balance. C57BL/6 (B6) mice were treated with ovalbumin to establish an allergic asthma (AA) model and subjected to IL-27 overexpression (OV) and empty vector (EV). Hematoxylin-eosin (HE) staining was performed to observe lung tissue inflammation. Flow cytometry was carried out to evaluate the percentage of Th9, Th1, and Th2 cells. The expression of IL-27, IL-27R, IL-9, T-bet, IFN-γ, and IgE was evaluated by quantitative...

In this work we theoretically study the exceptional points and reflection spectra characteristics of a grating coupled metal-insulator-metal heterostructure, which is a non-Hermitian system. Our results show that by selecting suitable geometrical parameters with grating periodicity @150 nm, that satisfy zero reflection condition, double exceptional points appear in a mode bifurcation regime. Furthermore, the thickness of partition metal layer between two cavities plays an important role in controlling the reflection properties of the heterostructure. There...

Additional file 3: Table S2. The number of paired ends reads for the ferret lung samples.

Additional file 4: Table S3. Complete annotation GO TERMS in smoke exposed ferrets compared to controls.

Additional file 5: Table S4. Complete list (52) of DEGs in common between ferret, mice, and human transcriptome.

Additional file 5: Table S4. Complete list (52) of DEGs in common between ferret, mice, and human transcriptome.

Additional file 3. The potential binding partners of RalA identified by immunoprecipitation-LC-MS.

Recent studies have shown that the use of mesenchymal stem/stromal cells (MSCs) may be a promising strategy for treating spinal cord injury (SCI). This study aimed to explore the effectiveness of human umbilical cord-derived MSCs (hUC-MSCs) with different administration routes and dosages on SCI rats. Following T10-spinal cord contusion in Sprague-Dawley rats (N = 60), three different dosages of hUC-MSCs were intrathecally injected into rats (SCI-ITH) after 24 h. Intravenous injection of hUC-MSCs (SCI-i.v.) and...

Supplemental material, sj-docx-1-cll-10.1177_09636897221139734 for Therapeutic Efficacy of Human Mesenchymal Stem Cells With Different Delivery Route and Dosages in Rat Models of Spinal Cord Injury by Guangyang Liu, Zhiling Zhao, Herui Wang, Chunhua Hao, Weiting Wang, Chenliang Zhang, Tiehua Wang, Xin Li, Jingjing Xi, Shaoyun Li, Haomiao Long, Yi Mi, Li Miao, Yaoyao Chen, Liqiang Xu, Libo Zheng, Hao Wang, Ning Ding, Fengmei Zhu, Qinggang Ge and Yongjun Liu in Cell Transplantation

Recent studies have shown that the use of mesenchymal stem/stromal cells (MSCs) may be a promising strategy for treating spinal cord injury (SCI). This study aimed to explore the effectiveness of human umbilical cord-derived MSCs (hUC-MSCs) with different administration routes and dosages on SCI rats. Following T10-spinal cord contusion in Sprague-Dawley rats (N = 60), three different dosages of hUC-MSCs were intrathecally injected into rats (SCI-ITH) after 24 h. Intravenous injection of hUC-MSCs (SCI-i.v.) and...

Tissue RNA was isolated from fresh mouse colon sample by Trizol reagent. Total RNA (1 µg) was reverse-transcribed using a reverse transcriptase kit (TakaRa, cat. RR036A-1, Tokyo, Japan). Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnolog, cat. 11201ES03, Shanghai, China) was used for Real-time qPCR. The comparative cycle threshold (Ct) method (2−ΔΔCT) was used to calculate relative expression, β-actin was used as the internal reference. We evaluated immunostaining by multiplying the intensity score and proportion...

Additional file 1: Supplementary figures.

Abstract Background Ventilatory power is a novel index which could reflect both ventilation efficiency and peripheral blood flow. However, its clinical value in pulmonary hypertension (PH) is rarely discussed. In the present study, we aimed to investigate the diagnostic and prognostic value of ventilatory power as well as its association with disease severity in PH. Methods Consecutive patients with normal hemodynamics and patients diagnosed with PH between September, 2012 and December, 2020 in Fuwai hospital...

Additional file 1. The quantitative details and statistics of histological analysis in the present study.Table 1 Statistics of H&E staining;Table 2 Statistics of Masson’s staining;Table 3 Statistics of IHC staining for CK19; Table 4 Statistics of IHC staining forα-SMA.

Abstract Background Clonorchiasis caused by Clonorchis sinensis is a zoonotic parasitic disease characterized by cholangitis, biliary proliferation, biliary fibrosis, and even cholangiocarcinoma. Our previous study showed that the expression of interleukin (IL)-33 is increased in both humans and mice infected by C. sinensis, suggesting that IL-33 is potentially involved in the pathogenesis of clonorchiasis. However, the roles and potential mechanism of IL-33 underlying remain unknown. Methods Wild-type (WT) and IL-33 knockout (KO) mice (BALB/c female...

Additional file 2: Supplemental Table 1. Resources used for our coronavirus disease-related data collection.

Additional file 2: Supplemental Table 1. Resources used for our coronavirus disease-related data collection.

Abstract Background The current COVID-19 pandemic and the previous SARS/MERS outbreaks of 2003 and 2012 have resulted in a series of major global public health crises. We argue that in the interest of developing effective and safe vaccines and drugs and to better understand coronaviruses and associated disease mechenisms it is necessary to integrate the large and exponentially growing body of heterogeneous coronavirus data. Ontologies play an important role in standard-based knowledge and data representation,...

Abstract Despite many nano-based strategies devoted to delivering cisplatin for tumor therapy, its clinical benefits are compromised by poor tissue penetration and limited DNA adducts formation of the drug. Herein, a cisplatin loading nanomotor based janus structured Ag-polymer is developed for cisplatin delivery of deeper tissue and increased DNA adducts formation. The nanomotor displayed a self‐propelled tumor penetration fueled by hydrogen peroxide (H2O2) in tumor tissues, which is catalytically decomposed into a large amount of...

Supplemental material, sj-pdf-1-jva-10.1177_11297298221139640 for Risk factors for in-stent restenosis in maintenance hemodialysis patients with central venous occlusive disease and biomechanical assessment of stents by Yang Yu, Yuqin Xiong, Tao Li, Jingyuan Zhou, Weijie Yan, Yan Xiong, Yu Chen and Ping Fu in The Journal of Vascular Access

Supplemental material, sj-pdf-2-jva-10.1177_11297298221139640 for Risk factors for in-stent restenosis in maintenance hemodialysis patients with central venous occlusive disease and biomechanical assessment of stents by Yang Yu, Yuqin Xiong, Tao Li, Jingyuan Zhou, Weijie Yan, Yan Xiong, Yu Chen and Ping Fu in The Journal of Vascular Access

Supplemental material, sj-pdf-3-jva-10.1177_11297298221139640 for Risk factors for in-stent restenosis in maintenance hemodialysis patients with central venous occlusive disease and biomechanical assessment of stents by Yang Yu, Yuqin Xiong, Tao Li, Jingyuan Zhou, Weijie Yan, Yan Xiong, Yu Chen and Ping Fu in The Journal of Vascular Access

Objectives:To investigate the risk factors and biomechanical mechanisms of in-stent restenosis (ISR) in central venous occlusive disease (CVOD).Patients and methods:This retrospective study consecutively included 77 maintenance hemodialysis (MHD) patients with CVOD who received the first percutaneous transluminal angioplasty with stenting (PTS) due to symptomatic CVOD in a tertiary hospital. The mean age was 59.7 ± 14.0 years, and 51.9% of patients were male. The clinical characteristics, occurrence of ISR and patency rates were recorded. Finite...