Supplemental material, sj-pdf-1-jva-10.1177_11297298221139640 for Risk factors for in-stent restenosis in maintenance hemodialysis patients with central venous occlusive disease and biomechanical assessment of stents by Yang Yu, Yuqin Xiong, Tao Li, Jingyuan Zhou, Weijie Yan, Yan Xiong, Yu Chen and Ping Fu in The Journal of Vascular Access

Objectives:To investigate the risk factors and biomechanical mechanisms of in-stent restenosis (ISR) in central venous occlusive disease (CVOD).Patients and methods:This retrospective study consecutively included 77 maintenance hemodialysis (MHD) patients with CVOD who received the first percutaneous transluminal angioplasty with stenting (PTS) due to symptomatic CVOD in a tertiary hospital. The mean age was 59.7 ± 14.0 years, and 51.9% of patients were male. The clinical characteristics, occurrence of ISR and patency rates were recorded. Finite...

Tissue RNA was isolated from fresh mouse colon sample by Trizol reagent. Total RNA (1 µg) was reverse-transcribed using a reverse transcriptase kit (TakaRa, cat. RR036A-1, Tokyo, Japan). Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnolog, cat. 11201ES03, Shanghai, China) was used for Real-time qPCR. The comparative cycle threshold (Ct) method (2−ΔΔCT) was used to calculate relative expression, β-actin was used as the internal reference. We evaluated immunostaining by multiplying the intensity score and proportion...

Additional file 1. List of KSA panel members.

Abstract Background Brain arteriovenous malformation (AVM) is an important cause of hemorrhagic stroke in young adults, which can lead to severe neurological impairment. The registry of Multimodality treatment for brain ArTeriovenous malformation in mainland CHina (MATCH) is a national prospective registry to identify the natural history of AVMs in Asian population; to investigate traditional and emerging hemorrhagic predictors; and to explore the superiority of the multidisciplinary assessment in improving the long-term outcomes. Methods Consecutive AVM...

Our study aimed to compare the predictive value of the COPD Assessment Test (CAT) score at baseline and short-term change in CAT for future exacerbations in chronic obstructive pulmonary disease (COPD) patients. This was a multicentre prospective study. Patients with COPD were recruited into the study and followed up for one year. CAT score and exacerbation in the previous year were collected at baseline. Change in CAT was defined as CAT score changing between baseline...

Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into...

Severe Community Acquired Pneumonia (SCAP) challenges public health globally. Considerable improvements in molecular pathogen testing emerged in the last few years. Our prospective study combinedly used traditional culture, antigen tests, PCR and mNGS in SCAP pathogen identification with clinical outcomes. From June 2018 to December 2019, we conducted a multi-centre prospective study in 17 hospitals of SCAP patients within 48 hours of emergency room stay or hospitalization in China. All clinical data were uploaded into...

α-Fluorinated chalcones were prepared and evaluated for their cell growth inhibitory properties against six human cancer cell lines. The most potent chalcone 4c demonstrated excellent selective toxicity against cancer cells versus normal human cells, with IC50 values at nanomolar concentration ranges against 5 cancer cell lines. A further study revealed that 4c could bind to the colchicine site of tubulin, disrupt the cell microtubule networks, and effectively inhibit tubulin polymerisation. Cellular-based mechanism studies elucidated that...

The traditional systemic chemotherapy through intravenous infusion of doxorubicin (DOX) has many side effects. The aim of this study was to develop a PLGA-based DOX-loaded implant and to evaluate the efficacy and drug metabolism distribution of the implant in intratumoral chemotherapy for osteosarcoma (OS). In this study, implants containing DOX, poly(d,l-lactide-co-glycolide), and polyethylene glycol 4000 were prepared by melt-molding method. Then, the antitumor activity and systemic drug distribution of the implants were tested in a...

Kenaf (Hibiscus cannabinus L.) is an industrial fiber crop with significant heterosis and tolerance to abiotic stresses. However, the mechanism underlying heterosis in kenaf under drought conditions is completely unknown. In this study, F1 hybrids were remarkably tolerant to drought stress. Through methylation-sensitive amplification polymorphism (MSAP) analysis, we highlighted that total DNA methylation level under drought decreased by 11.2% in F1 and increased by 29.7% and 13.1% in parents CP079 and CP071, respectively. Unlike parents,...

Additional file 1: Table S1. Scoring system for histological changes in the colon. Table S2. The sequences of primers used in this study. Figure S1. ERβ deficiency did not influence the sensorimotor function, memory function, or social interactions in mice following induced experimental colitis. (A) Nest score in the nest building test was performed among the four groups to detect the sensorimotor functions. (B) Spatial memory was assessed by percentage of spontaneous alterations in the...

Abstract Background Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood. Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice. Results In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β...

Abstract Background Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood. Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice. Results In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β...

Additional file 1. Multiplanar reconstruction of MRI using FS PD-TSE sequence of Case 1.

Hereditary hemorrhagic telangiectasis (HHT) and juvenile polyposis syndrome (JPS) are both relatively rare hereditary disorders. It has been reported that patients with SMAD4 mutations may suffer from both HHT and JPS, defined as JPS/HHT. To improve the understanding and diagnosis of these diseases, we herein report a case of a 17-year-old male with abdominal pain and hematochezia. Low-tension computed tomography (CT) of the small intestine showed intussusception. Combined with the patient’s medical history of nasal...

Abstract Background Among critically ill patients with acute kidney injury (AKI), earlier initiation of renal replacement therapy (RRT) may mitigate fluid accumulation and confer better outcomes among individuals with greater fluid overload at randomization. Methods We conducted a pre-planned post hoc analysis of the STandard versus Accelerated initiation of Renal Replacement Therapy in Acute Kidney Injury (STARRT-AKI) trial. We evaluated the effect of accelerated RRT initiation on cumulative fluid balance over the course of 14...

Additional file 11. High-quality, free open access resources for point-of-care ultrasound education

Additional file 2. Pre-course knowledge assessment of the learners

Additional file 5. Ultrasound-guided non-vascular access and advanced procedures

Additional file 7. Hemodynamic assessment of a critically ill patient using point-of-care ultrasound

Additional file 8. Focused thoracic imaging in a critically ill patient

Additional file 9. Focused assessment using sonography in trauma

Additional file 1. Supplemental methods, tables, and figures.

Abstract Background Artificial intelligence (AI) algorithms are capable of automatically detecting contouring boundaries in medical images. However, the algorithms impact on clinical practice of cervical cancer are unclear. We aimed to develop an AI-assisted system for automatic contouring of the clinical target volume (CTV) and organs-at-risk (OARs) in cervical cancer radiotherapy and conduct clinical-based observations. Methods We first retrospectively collected data of 203 patients with cervical cancer from West China Hospital. The proposed method named...