The fitted trees for evolutionary analyses using MEME, SLAC, FUBAR method

Supplemental Material for Association between serum uric acid level and systemic lupus erythematosus kidney outcome: An observational study in Southern Chinese population and a meta-analysis by Yipeng Han, Xiaoxi Lu, Shibai Xiao, Jiayu Qin, Lijuan Zheng, Yi Feng, Yujie Cai, Rong Qiu, Qin Huang, and Min Yang in Lupus

Enterovirus 76 (EV-A76) is a serotype of enterovirus A and has been rarely reported. In this paper, we present the genetic characteristics of 15 EV-A76 isolates reported to circulate in the Xinjiang Uighur autonomous region of China in 2011. Sequence analysis revealed that all Chinese EV-A76 isolates had high similarity (> 98.3%) in the VP1 region, and five Chinese EV-A76 isolates were selected for whole genome sequencing based on VP1 nucleotide divergence. Similarity plots and...

Supplemental material, sj-docx-6-taj-10.1177_20406223221143245 for Chest X-ray features facilitate screening for pulmonary hypertension caused by fibrosing mediastinitis by Mingfang Zhou, Bo Li, Yaling Chen, Aqian Wang, Yining Zhu, Yu Li, Hongling Su, Jingchun Fan, Yan Zhang and Yunshan Cao in Therapeutic Advances in Chronic Disease

Supplemental material, sj-png-2-taj-10.1177_20406223221143245 for Chest X-ray features facilitate screening for pulmonary hypertension caused by fibrosing mediastinitis by Mingfang Zhou, Bo Li, Yaling Chen, Aqian Wang, Yining Zhu, Yu Li, Hongling Su, Jingchun Fan, Yan Zhang and Yunshan Cao in Therapeutic Advances in Chronic Disease

Background:Misdiagnosis and underdiagnosis of pulmonary hypertension caused by fibrosing mediastinitis (PH-FM) are considerably prevalent due to unspecific symptoms and as well as the lack of awareness of this fatal disease.Objectives:The aim of this study was to evaluate the diagnostic accuracy of the chest X-ray (CXR) for screening the patients with PH-FM from those with pulmonary hypertension (PH).Design:This was a retrospective observational cohort study.Methods:The patients with suspected PH were recruited between October 2014 and October 2020....

Objective: Motor vehicle crashes result in egregious personal injury, mortality, and economic cost but are relatively rare in naturalistic observations. There is, however, evidence of strong relationships between crashes and less severe (but more common) “surrogate” events (e.g., near-crashes). Despite this strong relationship, there can still be some important differences in findings when these surrogate events are investigated in lieu of, or combined with, crashes. Therefore, it is relevant to describe and quantify differences between...

The shallow-water hydrothermal vent system of Kueishan Island has been described as one of the world's most acidic and sulfide-rich marine habitats. The only recorded metazoan species living in the direct vicinity of the vents is Xenograpsus testudinatus, a brachyuran crab endemic to marine sulfide-rich vent systems. Despite the toxicity of hydrogen sulfide, X. testudinatus occupies an ecological niche in a sulfide-rich habitat, with the underlying detoxification mechanism remaining unknown. Using laboratory and field-based experiments,...

Licorice is a product with the same origin as medicine and food and is widely used in traditional Chinese medicine and the food, cosmetics and tobacco industries. With the increasing demand for licorice in the herbal market, there is also increasing concern about complex varieties and quality deterioration. Considering the importance of the quality and safety of commercial licorice products, we used DNA barcoding, HPLC and colorimetry to distinguish and evaluate commercial licorice products. Here,...

We and others have previously demonstrated that exposure to nickel nanoparticles (Nano-Ni) caused fibrogenic and carcinogenic effects; however, the underlying mechanisms are still not fully understood. This study aimed to investigate the effects of Nano-Ni on epithelial-mesenchymal transition (EMT) in human bronchial epithelial cells (BEAS-2B) and its underlying mechanisms since EMT is involved in both cancer pathogenesis and tissue fibrosis. Our results showed that exposure to Nano-Ni, compared to the control Nano-TiO2, caused a remarkable...

Colorectal cancer (CRC) is a common gastrointestinal cancer with high incidence and mortality rates. CRC may be associated with regulation of circulating nucleotides. This study aimed to evaluate the serum levels of nucleotide-metabolizing enzymes (ATPase and AMPase) in patients with CRC and to explore the clinical diagnostic value of these enzymes. The gene set variation analysis (GSVA) score of the ATP-adenosine signature was calculated using tumor samples from The Cancer Genome Atlas (TCGA). ATP-adenosine signaling...

Although the use of enclosed learning space appears to influence both attention and learning in undergraduates, this issue has rarely been investigated. This study used four types of desktop spaces with different degrees of enclosure, namely, unenclosed, enclosed on one side, enclosed on two sides and enclosed on three sides, with enclosure degrees of 0, 25, 50 and 75%, respectively. Subjective evaluations, attention tests and eye tracking were used to analyze the effects of the...

In this work, the influence of sand impingements on cathodic protection (CP) of marine carbon steel in natural sea water was studied in comparison with the CP performances in static and flowing sea water without sand particles. Results show that the propagation of local active anodic dissolution at the steel inclusions could be totally inhibited by providing sufficient cathodic current in the sea water free of sand particles. However, the local ‘CP shielding’, potential fluctuation...

Bilateral aid procurement is politicised and strongly favours suppliers from donor countries. Does multilateral development assistance eliminate the procurement bias favouring donor countries because international bureaucrats make procurement decisions? Existing evidence from the World Bank, which delegates procurement responsibilities to aid recipient countries, cannot answer our theoretical question. Using official data from 20 UN organisations during the 2013–2018 period and applying regression and mediation analysis, we find that the procurement of international organisations still favours...

Complex disorders usually affect multiple symptom domains measured by several outcomes. The importance of these outcomes is often different among patients. Current approaches integrate multiple outcomes without considering patient preferences at the individual level. In this article, we propose a new composite Desirability of Outcome Ranking (DOOR) that integrates individual level ranking of outcome importance and define a winning probability measuring the overall treatment effect. Stratified randomization can be performed based on the participants’ baseline...

Monitoring brain activity is a novel development for hazard recognition in the construction industry. However, very few empirical studies have investigated the causal connections within the brain. This study aimed to explore the brain connectivity of construction workers during hazard recognition. Electroencephalogram data were collected from construction workers to perform image-based hazard recognition tasks. The Granger causality-based adaptive directed transfer function was used to simulate directed and time-variant information flow across the observed brain activity...

Due to ever increasing use of telecommunication and electronic devices in offshore and stealth technology sectors, developing highly-efficient electromagnetic interference (EMI) shielding materials with good thermal resistance are urgently required. In this paper, the effect of MWCNTs incorporation on the mechanical, interlaminar bonding and EMI performance of MWCNT/CF/PEEK composites were firstly studied. The introduction of interlaminar MWCNTs did not affect the tensile strength and modulus significantly at low loadings whilst slight decrease in them was...

Due to ever increasing use of telecommunication and electronic devices in offshore and stealth technology sectors, developing highly-efficient electromagnetic interference (EMI) shielding materials with good thermal resistance are urgently required. In this paper, the effect of MWCNTs incorporation on the mechanical, interlaminar bonding and EMI performance of MWCNT/CF/PEEK composites were firstly studied. The introduction of interlaminar MWCNTs did not affect the tensile strength and modulus significantly at low loadings whilst slight decrease in them was...

One new chamigrane sesquiterpene, antroalbol A (1), was isolated from the cultures of the higher fungus Antrodiella albocinnamomea. Its structure was established by means of spectroscopic methods, and the absolute configuration of 1 was confirmed by single crystal x-ray diffraction analysis. The compound was evaluated for its cytotoxicity against five human cancer cell lines, but no significant cytotoxicity was found.

Two new halogenated metabolites, laurenhalogens A (1) and B (2), along with four known ones (3–6), were isolated from the red alga Laurencia sp. The structures of 1 and 2 were determined by the means of UV, IR, MS, NMR and X-ray diffraction analysis. In addition, the antibacterial activities of 1–6 were also evaluated.

One new 6a,11a-dehydropterocarpan derivative, 6-O-methyl-anhydrotuberosin (1), one new 6a-hydroxypterocarpan, (6aR,11aR,11bR)-hydroxytuberosone (7), and seven known compounds including two 6a,11a-dehydropterocarpans (2 and 4), two coumestans (3 and 5), one isoflavonoid (6) and two other phenolic compounds (8 and 9) were isolated from the roots of Pueraria lobata. The structures of the isolated compounds were elucidated with spectroscopic and spectrometric methods (1 D and 2DNMR, HRESIMS). Compounds 1, 2, 4–5 showed potent LSD1 inhibitory activities with IC50 values...

Currently, human papillomavirus (HPV) vaccines are in short supply, so the development of HPV vaccines has a broad market prospect. The 3-, 9-, and 15-valent HPV vaccines developed by ourselves all contain HPV58-derived antigen components. It is important to detect HPV 58 during vaccine production. Here, we introduced a development process of HPV58 type-specific antibodies and a detection kit. Briefly, HPV58 L1-Virus Like Particles (VLPs) were used as antigens to immunize mice, followed by extraction...

SARS-CoV-2 variants continue to emerge facing established herd immunity. L452R, previously featured in the Delta variant, quickly emerged in Omicron subvariants, including BA.4/BA.5, implying a continued selection pressure on this residue. The underlying links between spike mutations and their selective pressures remain incompletely understood. Here, by analyzing 221 structurally characterized antibodies, we found that IGHV1-69-encoded antibodies preferentially contact L452 using germline-encoded hydrophobic residues at the tip of HCDR2 loop. Whereas somatic hypermutations or VDJ rearrangements...

Additional file 7. The correlation between the input DNA (cfDNA) concentration and the copy number of thereference gene POP4.

Supplemental material, sj-docx-1-taj-10.1177_20406223221135011 for Association between high-mobility group box 2 and subclinical hypertension-mediated organ damage in young adults by Jindong Wan, Gang Liu, Siwei Xia, Sen Liu, Yi Yang, Dan Wang, Jixin Hou, Xiaozhen Dai, Peng Zhou and Peijian Wang in Therapeutic Advances in Chronic Disease