719 Works

Iodine mediated synthesis of diaryl diselenides using SeO2 as a selenium source

Dileep Kommula, Qing Li, Siyang Ning, Wujun Liu, Qian Wang & Zongbao K. Zhao
A simple, eco-friendly and convenient procedure has been developed for the synthesis of diphenyl diselinides from readily available aryl boronic acids by reaction with SeO2 in the presence of iodine under simple conditions. The methodology is reported about 19 examples and applicable to a broad scope of aryl boronic acids containing electron-rich and electron-poor substituents. Apart from being milder and environmentally benign conditions, this synthetic protocol comprises a novel, reliable methodology to give good to...

Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients

Baoqing Sun, Ying Feng, Xiaoneng Mo, Peiyan Zheng, Qian Wang, Pingchao Li, Ping Peng, Xiaoqing Liu, Zhilong Chen, Huimin Huang, Fan Zhang, Wenting Luo, Xuefeng Niu, Peiyu Hu, Longyu Wang, Hui Peng, Zhifeng Huang, Liqiang Feng, Feng Li, Fuchun Zhang, Fang Li, Nanshan Zhong & Ling Chen
The emerging COVID-19 caused by SARS-CoV-2 infection poses severe challenges to global public health. Serum antibody testing is becoming one of the critical methods for the diagnosis of COVID-19 patients. We investigated IgM and IgG responses against SARS-CoV-2 nucleocapsid (N) and spike (S) protein after symptom onset in the intensive care unit (ICU) and non-ICU patients. 130 blood samples from 38 COVID-19 patients were collected. The levels of IgM and IgG specific to N and...

GIT1 overexpression promotes epithelial-mesenchymal transition and predicts poor prognosis in hepatocellular carcinoma

Guifu Wang, Xuesong Bai, Guoqing Jiang, Shengjie Jin, Qian Wang, Aoqing Wang, Rui Peng, Aiwu Ke & Dousheng Bai
Globally, hepatocellular carcinoma (HCC) is one of the most common causes of cancer-associated mortalities. It has a high rate of metastasis and recurrence, which predict a poor prognosis. G-protein-coupled receptor (GPCR)-kinase interacting protein-1 (GIT1) is a multifunctional scaffold protein that mediates the progression of various tumors. Studies have correlated GIT1 with HCC, however, these correlations have not been fully elucidated. Therefore, we aimed at evaluating the expression of GIT1 in HCC tissues and cells, and...

Dynamic Dissection of Dynein and Kinesin-1 Cooperatively Mediated Intercellular Transport of Porcine Epidemic Diarrhea Coronavirus along Microtubule Using Single Virus Tracking

Wei Hou, Wenjie Kang, Yangyang Li, Yanke Shan, Shouyu Wang & Fei Liu
It is now clear that the intercellular transport on microtubules by dynein and kinesin-1 motors has an important role in the replication and spread of many viruses. Porcine epidemic diarrhea virus (PEDV) is an enveloped, single-stranded RNA virus of the Coronavirus family, which can infect swine of all ages and cause severe economic losses in the swine industry. Elucidating the molecular mechanisms of the intercellular transport of PEDV through microtubule, dynein and kinesin-1 will be...

Dynamic Dissection of Dynein and Kinesin-1 Cooperatively Mediated Intercellular Transport of Porcine Epidemic Diarrhea Coronavirus along Microtubule Using Single Virus Tracking

Wei Hou, Wenjie Kang, Yangyang Li, Yanke Shan, Shouyu Wang & Fei Liu
It is now clear that the intercellular transport on microtubules by dynein and kinesin-1 motors has an important role in the replication and spread of many viruses. Porcine epidemic diarrhea virus (PEDV) is an enveloped, single-stranded RNA virus of the Coronavirus family, which can infect swine of all ages and cause severe economic losses in the swine industry. Elucidating the molecular mechanisms of the intercellular transport of PEDV through microtubule, dynein and kinesin-1 will be...

Expanded 3D nanofibre sponge scaffolds by gas-foaming technique enhance peripheral nerve regeneration

Feng Rao, Zhipeng Yuan, Ming Li, Fei Yu, Xingxing Fang, Baoguo Jiang, Yongqiang Wen & Peixun Zhang
Peripheral nerve injury has troubled clinical doctors for many years. To obtain better function recovery of peripheral nerve repair at the base of hollow nerve guidance conduit (NGC), many NGCs with fillers were developed in the application of tissue-engineered nerve graft. In this study, expanded 3D nanofibre sponge scaffolds with orientation and porosity were first fabricated by electrospinning and gas-foaming technique. Polylactic acid (PLA)/silk fibroin nanofibre sponge scaffolds were prepared as filler to construct 3D...

Acute kidney injury in idiopathic membranous nephropathy with nephrotic syndrome

Tianxin Chen, Ying Zhou, Xinxin Chen, Bo Chen & Jingye Pan
The impact of acute kidney injury (AKI) on the progression of renal function in idiopathic membranous nephropathy (iMN) with nephrotic syndrome (NS) patients have not yet been reported, we sought to investigate the incidence, clinical features and prognosis of AKI in iMN with NS patients and determine clinical predictors for progression from AKI to advanced chronic kidney disease (CKD) stage. We analyzed clinical and pathological data of iMN with NS patients retrospectively collected from Jan...

Silencing circular RNA circ_0054537 and upregulating microRNA-640 suppress malignant progression of renal cell carcinoma via regulating neuronal pentraxin-2 (NPTX2)

Long Pei, Xianqiang Lv, Gaopei Jia, Xiaoliang Tan, Ming Li & Aili Zhang
Hsa_circ_0054537 (circ_0054537) is a novel tumor-related circular RNA in renal cell carcinoma (RCC), and we intended to ascertain its dysregulation and functions in RCC malignant progression, as well as the underlying mechanism via serving as competing endogenous RNA (ceRNA). In this research, using real-time quantitative PCR, we found circ_0054537 was upregulated in RCC tissues and cells, and distributed throughout the cytoplasm. Then, functional effects of circ_0054537 in RCC were detected using cell counting kit-8, transwell,...

Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients

Baoqing Sun, Ying Feng, Xiaoneng Mo, Peiyan Zheng, Qian Wang, Pingchao Li, Ping Peng, Xiaoqing Liu, Zhilong Chen, Huimin Huang, Fan Zhang, Wenting Luo, Xuefeng Niu, Peiyu Hu, Longyu Wang, Hui Peng, Zhifeng Huang, Liqiang Feng, Feng Li, Fuchun Zhang, Fang Li, Nanshan Zhong & Ling Chen
The emerging COVID-19 caused by SARS-CoV-2 infection poses severe challenges to global public health. Serum antibody testing is becoming one of the critical methods for the diagnosis of COVID-19 patients. We investigated IgM and IgG responses against SARS-CoV-2 nucleocapsid (N) and spike (S) protein after symptom onset in the intensive care unit (ICU) and non-ICU patients. 130 blood samples from 38 COVID-19 patients were collected. The levels of IgM and IgG specific to N and...

Paeoniflorin reduce luxS/AI-2 system-controlled biofilm formation and virulence in Streptococcus suis

Jinpeng Li, Qingying Fan, Manyu Jin, Chenlong Mao, Hui Zhang, Xiaoling Zhang, Liyun Sun, Daniel Grenier, Li Yi, Xiaogai Hou & Yang Wang
Streptococcus suis (S. suis), more specifically serotype 2, is a bacterial pathogen that threatens the lives of pigs and humans. Like many other pathogens, S. suis exhibits quorum sensing (QS) system-controlled virulence factors, such as biofilm formation that complicates treatment. Therefore, impairing the QS involving LuxS/AI-2 cycle in S. suis, may be a promising alternative strategy for overcoming S. suis infections. In this study, we investigated paeoniflorin (PF), a monoterpenoid glycoside compound extracted from peony,...

Mitochondrial-bound hexokinase 1 can affect the glucolipid metabolism and reactive oxygen species production in goose fatty liver

Minmeng Zhao, Qian Wang, Long Liu, Tuoyu Geng & Daoqing Gong
To investigate the functions of hexokinase 1 (HK1) in the formation of goose fatty liver, a total of 40 healthy 63-day-old male Landes geese were selected and randomly assigned to a control group and an overfeeding treatment. In addition, the overexpression or RNA interference assay of HK1, and transcriptome analysis after HK1 overexpression were performed in the goose primary hepatocytes. Data showed that the mRNA expression of hepatic HK1 was upregulated in overfed treatment compared...

Mitochondrial-bound hexokinase 1 can affect the glucolipid metabolism and reactive oxygen species production in goose fatty liver

Minmeng Zhao, Qian Wang, Long Liu, Tuoyu Geng & Daoqing Gong
To investigate the functions of hexokinase 1 (HK1) in the formation of goose fatty liver, a total of 40 healthy 63-day-old male Landes geese were selected and randomly assigned to a control group and an overfeeding treatment. In addition, the overexpression or RNA interference assay of HK1, and transcriptome analysis after HK1 overexpression were performed in the goose primary hepatocytes. Data showed that the mRNA expression of hepatic HK1 was upregulated in overfed treatment compared...

Long non-coding RNA terminal differentiation-induced non-coding RNA regulates cisplatin resistance of choroidal melanoma by positively modulating extracellular signal-regulated kinase 2 via sponging microRNA-19b-3p

Wei Li, Aiqin Nie, Longyu Jin, Yubo Cui, Ning Xie & Gaohua Liang
In the present study, we aimed to investigate the role of long non-coding RNA terminal differentiation-induced non-coding RNA (TINCR) in cisplatin (DDP) resistance of choroidal melanoma (CM) and the potential molecular mechanisms. CM and non-CM tissues were collected from 60 CM patients. DDP-resistant CM cells were obtained by selection with linearly increased DDP treatment. The expression levels of TINCR, microR-19b-3p (miR-19b-3p), and extracellular signal-regulated kinase 2 (ERK-2) were detected by quantitative real-time PCR. Cholecystokinin octapeptide...

Effect of wounding and wound age on infection of canola cotyledons by Leptosphaeria maculans, interacting with leaf wetness

Shuanglong Huang, Chun Zhai, Zhongwei Zou, Fei Liu, Paula Parks, Linda Mcgregor, W. G. Dilantha Fernando & Gary Peng
Blackleg, caused by the fungus Leptosphaeria maculans, is an important disease of canola. Both ascospores and pycnidiospores of the fungus can infect intact leaves under conditions of extended leaf wetness, with infection then progressing further into the stem. In western Canada, spring is typically cool and dry and additional factors may be involved for successful infection. This study was designed to assess the effect of wounding and wound age on cotyledon infection, to evaluate the...

Transcriptomic analysis of the innate immune signatures of a SARS-CoV-2 protein subunit vaccine ZF2001 and an mRNA vaccine RRV

Qian Wang, Ziyang Song, Jinghuan Yang, Qian He, Qunying Mao, Yu Bai, Jianyang Liu, Chaoqiang An, Xujia Yan, Bopei Cui, Lifang Song, Dong Liu, Miao Xu & Zhenglun Liang
Analysis of large-scale gene expression post vaccination can provide an overview of immune responses. We used transcriptional approaches to comprehensively analyze the innate immune response signatures elicited by protein subunit (PS) vaccine ZF2001 and an mRNA vaccine named RRV. A fine-grained time-dependent dissection of large-scale gene expression post immunization revealed that ZF001 induced MHC class II-related genes, including cd74 and H2-Aa, more expeditiously than the RRV. Notably, the RRV induced MHC class I-related genes such...

Evolutionary analysis of MIR156/157 family during floral induction in walnut (Juglans regia L.)

Xing Yuan, Jianxin Niu, Li Zhou, Shaowen Quan, Li Ma & Hang Xu
The miR156/157 family is widely identified in various plant species and plays important regulatory roles in floral induction and stress response. However, information regarding evolution of miR156/157s and their functions during floral induction in walnut (Juglans regia) remains unknown. In this study, four precursors and four mature miR156/157s were identified in J. regia via small RNA sequencing. We constructed phylogenetic trees for precursor sequences of miR156/157s from different plants, as well as J. regia. The...

Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells

Wenwen Liu, Min Chen, Chao Liu, Liying Wang, Huafang Wei, Ruidan Zhang, Zhengxing Ren, Yinghong Chen, Mengcheng Luo, Jianguo Zhao, Hongwei Jiang, Fei Gao & Wei Li
Primary ovarian insufficiency (POI), also known as premature ovarian failure, is an ovarian defect in humans characterized by the premature depletion of ovarian follicles before the age of 40. However, the mechanisms underlying POI remain largely unknown. Here, we show that knockout of Epg5 (ectopic P-granules autophagy protein 5 homolog (C. elegans)) results in subfertility in female mice, which exhibit a POI-like phenotype. Single-cell RNA sequencing analysis revealed that the knockout of Epg5 affected the...

Additional file 1 of Genomic Architecture of Yield Performance of an Elite Rice Hybrid Revealed by its Derived Recombinant Inbred Line and Their Backcross Hybrid Populations

Fan Zhang, Conghe Zhang, Xiuqin Zhao, Shuangbing Zhu, Kai Chen, Guixiang Zhou, Zhichao Wu, Min Li, Tianqing Zheng, Wensheng Wang, Zhi Yan, Qinyong Fei, Zhikang Li, Jinjie Chen & Jianlong Xu
Additional file 1: Table S1. Phenotypic performance and heterosis of traits in Q9311B/WSSM RILs and backcross F1s (Q9311A × RIL).

Additional file 7 of Genomic Architecture of Yield Performance of an Elite Rice Hybrid Revealed by its Derived Recombinant Inbred Line and Their Backcross Hybrid Populations

Fan Zhang, Conghe Zhang, Xiuqin Zhao, Shuangbing Zhu, Kai Chen, Guixiang Zhou, Zhichao Wu, Min Li, Tianqing Zheng, Wensheng Wang, Zhi Yan, Qinyong Fei, Zhikang Li, Jinjie Chen & Jianlong Xu
Additional file 7: Table S4. Epistasis QTLs for 12 traits in the three environments.

Additional file 9 of Genomic Architecture of Yield Performance of an Elite Rice Hybrid Revealed by its Derived Recombinant Inbred Line and Their Backcross Hybrid Populations

Fan Zhang, Conghe Zhang, Xiuqin Zhao, Shuangbing Zhu, Kai Chen, Guixiang Zhou, Zhichao Wu, Min Li, Tianqing Zheng, Wensheng Wang, Zhi Yan, Qinyong Fei, Zhikang Li, Jinjie Chen & Jianlong Xu
Additional file 9: Fig. S4. Whole-genome variation of two parental lines of QYSM. a, Density and chromosome distribution of polymorphic SNPs between Q9311B and WSSM. b, The principal component analysis. c, Phylogenetic positions of two parental lines (Q9311B and WSSM) in the neighbor-joining tree of 1,946 rice accessions from 3 K-RG, in which the known information on the clades of 3 K-RG is indicated.

Genomic Architecture of Yield Performance of an Elite Rice Hybrid Revealed by its Derived Recombinant Inbred Line and Their Backcross Hybrid Populations

Fan Zhang, Conghe Zhang, Xiuqin Zhao, Shuangbing Zhu, Kai Chen, Guixiang Zhou, Zhichao Wu, Min Li, Tianqing Zheng, Wensheng Wang, Zhi Yan, Qinyong Fei, Zhikang Li, Jinjie Chen & Jianlong Xu
Abstract Background Since its development and wide adoption in China, hybrid rice has reached the yield plateau for more than three decades. To understand the genetic basis of heterosis in rice and accelerate hybrid rice breeding, the yield performances of the elite rice hybrid, Quan-you-si-miao (QYSM) were genetically dissected by whole-genome sequencing, large-scale phenotyping of 1061 recombined inbred lines (RILs) and 1061 backcross F1 (BCF1) hybrids derived from QYSM’s parents across three environments and gene-based...

Additional file 1 of Age- and sex-related differences in the retinal capillary plexus in healthy Chinese adults

Binbin Su, Xiaoxuan Zhu, Kai Yang, Yunfan Xiao, Chunmei Li, Keai Shi, Jia Qu, Fan Lu, Ming Li & Lele Cui
Additional file 1: Figure S1. Vessel densities of the whole measured area, parafovea, and four quadrants (temporal, superior, nasal, and inferior) in the (a, c) SCP and (b, d) DCP measured with optical coherence tomography angiography stratified by age in males and females. Table S1. Baseline characteristics of male participants in the study. Table S2. Baseline characteristics of female participants in the study. Table S3. Vessel densities of the whole measured area, parafovea, and four...

Additional file 10 of Circulating tumor DNA integrating tissue clonality detects minimal residual disease in resectable non-small-cell lung cancer

Siwei Wang, Ming Li, Jingyuan Zhang, Peng Xing, Min Wu, Fancheng Meng, Feng Jiang, Jie Wang, Hua Bao, Jianfeng Huang, Binhui Ren, Mingfeng Yu, Ninglei Qiu, Houhuai Li, Fangliang Yuan, Zhi Zhang, Hui Jia, Xinxin Lu, Shuai Zhang, Xiaojun Wang, Youtao Xu, Wenjia Xia, Tongyan Liu, Weizhang Xu, Xinyu Xu … & Rong Yin
Additional file 10. Figure S7: Prognostic value of presurgical ctDNA detection. A). The recurrence-free survival analysis of patients stratified by presurgical ctDNA detection. B). The multi-variant Cox regression for presurgical ctDNA detection. Abbreviations: RFS – recurrence-free survival, LNM – lymph node metastasis, LUAD – lung adenocarcinoma, LUSC - lung squamous-cell carcinoma.

Additional file 13 of Circulating tumor DNA integrating tissue clonality detects minimal residual disease in resectable non-small-cell lung cancer

Siwei Wang, Ming Li, Jingyuan Zhang, Peng Xing, Min Wu, Fancheng Meng, Feng Jiang, Jie Wang, Hua Bao, Jianfeng Huang, Binhui Ren, Mingfeng Yu, Ninglei Qiu, Houhuai Li, Fangliang Yuan, Zhi Zhang, Hui Jia, Xinxin Lu, Shuai Zhang, Xiaojun Wang, Youtao Xu, Wenjia Xia, Tongyan Liu, Weizhang Xu, Xinyu Xu … & Rong Yin
Additional file 13. Supplementary results.

Additional file 1 of Circulating tumor DNA integrating tissue clonality detects minimal residual disease in resectable non-small-cell lung cancer

Siwei Wang, Ming Li, Jingyuan Zhang, Peng Xing, Min Wu, Fancheng Meng, Feng Jiang, Jie Wang, Hua Bao, Jianfeng Huang, Binhui Ren, Mingfeng Yu, Ninglei Qiu, Houhuai Li, Fangliang Yuan, Zhi Zhang, Hui Jia, Xinxin Lu, Shuai Zhang, Xiaojun Wang, Youtao Xu, Wenjia Xia, Tongyan Liu, Weizhang Xu, Xinyu Xu … & Rong Yin
Additional file 1. Table S1: Patient demography.

Registration Year

  • 2023
    51
  • 2022
    593
  • 2021
    41
  • 2020
    16
  • 2019
    9
  • 2018
    1
  • 2017
    3
  • 2014
    2
  • 2013
    1
  • 2012
    1

Resource Types

  • Text
    302
  • Dataset
    165
  • Collection
    126
  • Image
    112
  • Other
    8
  • Audiovisual
    6

Affiliations

  • Institute of Hydrobiology
    719
  • University of Chinese Academy of Sciences
    691
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    559
  • Shanghai Jiao Tong University
    483
  • Shandong University
    420
  • Zhejiang University
    396
  • Peking University
    389
  • Capital Medical University
    387
  • Nanjing Medical University
    368
  • Ministry of Education of the People's Republic of China
    365