31 Works

Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Abstract Background We aimed to assess the prevalence of Salmonella Typhi through DNA and IgM-antibody detection methods as a prelude to extended surveillance activities at sites in Ghana, Madagascar, and Ethiopia. Methods We performed species-specific real-time polymerase reaction (RT-PCR) to identify bacterial nucleic acid, and enzyme-linked immunosorbent assay (ELISA) for detecting HlyE/STY1498-, CdtB/STY1886-, pilL/STY4539- and Vi-antigens in blood and biopsy specimens of febrile and non-febrile subjects. We generated antigen-specific ELISA proxy cut-offs by change-point analyses,...

Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Abstract Background We aimed to assess the prevalence of Salmonella Typhi through DNA and IgM-antibody detection methods as a prelude to extended surveillance activities at sites in Ghana, Madagascar, and Ethiopia. Methods We performed species-specific real-time polymerase reaction (RT-PCR) to identify bacterial nucleic acid, and enzyme-linked immunosorbent assay (ELISA) for detecting HlyE/STY1498-, CdtB/STY1886-, pilL/STY4539- and Vi-antigens in blood and biopsy specimens of febrile and non-febrile subjects. We generated antigen-specific ELISA proxy cut-offs by change-point analyses,...

Termite diversity is resilient to land-use change

Gabriel Quansah, Gabriel Quansah, Stephen Adu-Bredu, Vincent Logah, Yadvinder Malhi, Paul Eggleton & Catherine Parr
Cocoa is an important crop for Ghana’s economy, contributing 25% of Gross Domestic Product (GDP). The crop, however, is mainly cultivated on forest-derived soils and is a major cause of land-use change. Termites are an important biological component of tropical ecosystems providing numerous ecosystem services. Previous studies have indicated that termites are sensitive to forest disturbance and decrease in richness and abundance across land-use intensification gradients, with consequences for the essential services that they provide....

Additional file 1 of The incidence of TB and MDR-TB in pediatrics and therapeutic options: a systematic review

Sheetal Harichander, Ebenezer Wiafe, Kofi Boamah Mensah, Varsha Bangalee & Frasia Oosthuizen
Additional file 1. Search strategy.

Additional file 2 of The incidence of TB and MDR-TB in pediatrics and therapeutic options: a systematic review

Sheetal Harichander, Ebenezer Wiafe, Kofi Boamah Mensah, Varsha Bangalee & Frasia Oosthuizen
Additional file 2. Quality assessment tool.

Additional file 1 of Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Additional file 1. Location of sites in Ethiopia [1A], Ghana [1B] and Madagascar [1C]. Notes: The location of each site is indicated as a black dot and the site’s name in red font; Ethiopia (Figure 1A): nine regional states (black italic, capital letters) and two chartered cities (black italic, lower letters) are shown;SNNPR=Southern Nations, Nationalities and People’s Region; Ghana (Figure 1B): ten regions (black italic, capital letters) are shown; Madagascar (Figure 1C): six provinces (black...

Additional file 2 of Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Additional file 2. Distribution of ELISA OD values by antigen.

Strengthening climate adaptation in the northern region of Ghana: insights from a stakeholder analysis

Enoch Yeleliere, Andy Bonaventure Nyamekye, Philip Antwi-Agyei & Emmanuel Frimpong Boamah
Climate change significantly impacts agriculture. Building an informed and collaborative process among stakeholders remains vital in minimizing climate risks and building climate-adaptive and resilient agricultural systems in sub-Saharan Africa (SSA). However, stakeholders’ involvement and collaboration in agriculture decision-making are framed by institutional, power, and resource dynamics, often ignoring vulnerable stakeholders most susceptible to climate shocks. These collaborative dynamics are less studied in the climate adaptation literature, especially in the context of Ghana and SSA. This...

sj-docx-1-cpc-10.1177_10556656221135926 - Supplemental material for Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate

Waheed Awotoye, Peter A Mossey, Jacqueline B Hetmanski, Lord J J Gowans, Mekonen A Eshete, Wasiu L Adeyemo, Azeez Alade, Erliang Zeng, Olawale Adamson, Olutayo James, Azeez Fashina, Modupe O Ogunlewe, Thirona Naicker, Chinyere Adeleke, Tamara Busch, Mary Li, Aline Petrin, Abimbola Oladayo, Sami Kayali, Joy Olotu, Veronica Sule, Mohaned Hassan, John Pape, Emmanuel T Aladenika, Peter Donkor … & Azeez Butali
Supplemental material, sj-docx-1-cpc-10.1177_10556656221135926 for Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate by Waheed Awotoye, Peter A Mossey, Jacqueline B Hetmanski, Lord J J Gowans, Mekonen A Eshete and Wasiu L Adeyemo, Azeez Alade, Erliang Zeng, Olawale Adamson, Olutayo James, Azeez Fashina, Modupe O Ogunlewe, Thirona Naicker, Chinyere Adeleke, Tamara Busch, Mary Li, Aline Petrin, Abimbola Oladayo, Sami Kayali, Joy Olotu, Veronica Sule, Mohaned Hassan, John Pape,...

Additional file 1 of Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Additional file 1. Location of sites in Ethiopia [1A], Ghana [1B] and Madagascar [1C]. Notes: The location of each site is indicated as a black dot and the site’s name in red font; Ethiopia (Figure 1A): nine regional states (black italic, capital letters) and two chartered cities (black italic, lower letters) are shown;SNNPR=Southern Nations, Nationalities and People’s Region; Ghana (Figure 1B): ten regions (black italic, capital letters) are shown; Madagascar (Figure 1C): six provinces (black...

Additional file 1 of The incidence of TB and MDR-TB in pediatrics and therapeutic options: a systematic review

Sheetal Harichander, Ebenezer Wiafe, Kofi Boamah Mensah, Varsha Bangalee & Frasia Oosthuizen
Additional file 1. Search strategy.

Additional file 2 of The incidence of TB and MDR-TB in pediatrics and therapeutic options: a systematic review

Sheetal Harichander, Ebenezer Wiafe, Kofi Boamah Mensah, Varsha Bangalee & Frasia Oosthuizen
Additional file 2. Quality assessment tool.

Additional file 1 of Multi-drug resistant bacteria isolates from lymphatic filariasis patients in the Ahanta West District, Ghana

Bill Clinton Aglomasa, Cynthia Kyerewaa Adu-Asiamah, Samuel Opoku Asiedu, Priscilla Kini, Emmanuel Kobla Atsu Amewu, Kennedy Gyau Boahen, Solomon Wireko, Isaac Kingsley Amponsah, Yaw Duah Boakye, Vivian Etsiapa Boamah & Alexander Kwarteng
Additional file 1.

Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate

Waheed Awotoye, Peter A Mossey, Jacqueline B Hetmanski, Lord J J Gowans, Mekonen A Eshete, Wasiu L Adeyemo, Azeez Alade, Erliang Zeng, Olawale Adamson, Olutayo James, Azeez Fashina, Modupe O Ogunlewe, Thirona Naicker, Chinyere Adeleke, Tamara Busch, Mary Li, Aline Petrin, Abimbola Oladayo, Sami Kayali, Joy Olotu, Veronica Sule, Mohaned Hassan, John Pape, Emmanuel T Aladenika, Peter Donkor … & Azeez Butali
ObjectivesNovel or rare damaging mutations have been implicated in the developmental pathogenesis of nonsyndromic cleft lip with or without cleft palate (nsCL ± P). Thus, we investigated the human genome for high-impact mutations that could explain the risk of nsCL ± P in our cohorts.Materials and Methods:We conducted next-generation sequencing (NGS) analysis of 130 nsCL ± P case-parent African trios to identify pathogenic variants that contribute to the risk of clefting. We replicated this analysis...

Effectiveness of school-based interventions for preventing tobacco smoking initiation among young people in low- and middle-income countries: a systematic review protocol

Divine Darlington Logo, Yeetey Enuameh, George Adjei, Arti Singh, Emmanuel Nakua, Edward Dassah, Felix Boakye Oppong & Ellis Owusu-Dabo
Abstract Background Despite the commendable progress made globally in tobacco control, the world is falling short of achieving a 30% relative reduction in current tobacco use by 2025. The African region remains the least in the efforts in fighting the tobacco epidemic and is most exploited by the tobacco industry. Schools have been continuously used for over three decades as a setting for delivering youth smoking prevention programmes; however, the evidence of the effectiveness of...

Edge disturbance shapes liana diversity and abundance but not liana-tree interaction network patterns in moist semi-deciduous forests, Ghana

Patrick Addo-Fordjour
Edge disturbance can drive liana community changes and alter liana-tree interaction networks, with ramifications for forest functioning. Understanding edge effects on liana community structure and liana-tree interactions is therefore essential for forest management and conservation. We evaluated the response of liana community structure and the patterns of liana-tree interaction structure to forest edge in two moist semi-deciduous forests in Ghana (Asenanyo and Suhuma Forest Reserves: AFR and SFR, respectively). Liana community structure and liana-tree interactions...

Additional file 2 of Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Additional file 2. Distribution of ELISA OD values by antigen.

Additional file 1 of Multi-drug resistant bacteria isolates from lymphatic filariasis patients in the Ahanta West District, Ghana

Bill Clinton Aglomasa, Cynthia Kyerewaa Adu-Asiamah, Samuel Opoku Asiedu, Priscilla Kini, Emmanuel Kobla Atsu Amewu, Kennedy Gyau Boahen, Solomon Wireko, Isaac Kingsley Amponsah, Yaw Duah Boakye, Vivian Etsiapa Boamah & Alexander Kwarteng
Additional file 1.

sj-docx-1-cpc-10.1177_10556656221135926 - Supplemental material for Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate

Waheed Awotoye, Peter A Mossey, Jacqueline B Hetmanski, Lord J J Gowans, Mekonen A Eshete, Wasiu L Adeyemo, Azeez Alade, Erliang Zeng, Olawale Adamson, Olutayo James, Azeez Fashina, Modupe O Ogunlewe, Thirona Naicker, Chinyere Adeleke, Tamara Busch, Mary Li, Aline Petrin, Abimbola Oladayo, Sami Kayali, Joy Olotu, Veronica Sule, Mohaned Hassan, John Pape, Emmanuel T Aladenika, Peter Donkor … & Azeez Butali
Supplemental material, sj-docx-1-cpc-10.1177_10556656221135926 for Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate by Waheed Awotoye, Peter A Mossey, Jacqueline B Hetmanski, Lord J J Gowans, Mekonen A Eshete and Wasiu L Adeyemo, Azeez Alade, Erliang Zeng, Olawale Adamson, Olutayo James, Azeez Fashina, Modupe O Ogunlewe, Thirona Naicker, Chinyere Adeleke, Tamara Busch, Mary Li, Aline Petrin, Abimbola Oladayo, Sami Kayali, Joy Olotu, Veronica Sule, Mohaned Hassan, John Pape,...

Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate

Waheed Awotoye, Peter A Mossey, Jacqueline B Hetmanski, Lord J J Gowans, Mekonen A Eshete, Wasiu L Adeyemo, Azeez Alade, Erliang Zeng, Olawale Adamson, Olutayo James, Azeez Fashina, Modupe O Ogunlewe, Thirona Naicker, Chinyere Adeleke, Tamara Busch, Mary Li, Aline Petrin, Abimbola Oladayo, Sami Kayali, Joy Olotu, Veronica Sule, Mohaned Hassan, John Pape, Emmanuel T Aladenika, Peter Donkor … & Azeez Butali
ObjectivesNovel or rare damaging mutations have been implicated in the developmental pathogenesis of nonsyndromic cleft lip with or without cleft palate (nsCL ± P). Thus, we investigated the human genome for high-impact mutations that could explain the risk of nsCL ± P in our cohorts.Materials and Methods:We conducted next-generation sequencing (NGS) analysis of 130 nsCL ± P case-parent African trios to identify pathogenic variants that contribute to the risk of clefting. We replicated this analysis...

Farmers perceptions on trends of onset and session of rainfall in Northern Ghana

Francis Muthoni, Winifred Ayinpogbilla Atiah, Bekele Kotu, Fred Kizito & Leonard K. Amekudzi
The timing of onset and cession of rainfall has wide implications on the cropping calendar activities. This information is recorded by rainfall gauge stations that are sparse in northern Ghana. Data recorded from earth observing satellites can complement the sparse gauge stations. However, there is need to verify if the trends measured from satellite data is consistent with the experiences of farmers. The aim of this tool is to collect information on farmers perceptions on...

The incidence of TB and MDR-TB in pediatrics and therapeutic options: a systematic review

Sheetal Harichander, Ebenezer Wiafe, Kofi Boamah Mensah, Varsha Bangalee & Frasia Oosthuizen
Abstract Background Tuberculosis (TB) is considered one of the top 10 causes of death worldwide and the leading cause of death from a single infectious agent. Multidrug-resistant (MDR) TB can affect people of all age groups, including children (aged 0–15 years). However, very little is known about the extent of this problem in children. This systematic review aims to investigate the incidence of TB and drug-resistant (DR) TB among the pediatric population. It also reviews...

The incidence of TB and MDR-TB in pediatrics and therapeutic options: a systematic review

Sheetal Harichander, Ebenezer Wiafe, Kofi Boamah Mensah, Varsha Bangalee & Frasia Oosthuizen
Abstract Background Tuberculosis (TB) is considered one of the top 10 causes of death worldwide and the leading cause of death from a single infectious agent. Multidrug-resistant (MDR) TB can affect people of all age groups, including children (aged 0–15 years). However, very little is known about the extent of this problem in children. This systematic review aims to investigate the incidence of TB and drug-resistant (DR) TB among the pediatric population. It also reviews...

Additional file 3 of Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Additional file 3. Mean ELISA ODs by antigen. Notes: OD=optical density; red dot=mean of original data; blue dot=mean of each 1,000 bootstrap random samples; light red dashed line=upper and lower 95% confidence interval (CI) of original data; dark red dashed line=upper and lower 99% confidence interval (CI) of original data

Additional file 3 of Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia

Ursula Panzner, Ondari Daniel Mogeni, Yaw Adu-Sarkodie, Trevor Toy, Hyon Jin Jeon, Gi Deok Pak, Se Eun Park, Yeetey Enuameh, Ellis Owusu-Dabo, Trinh Van Tan, Abraham Aseffa, Mekonnen Teferi, Biruk Yeshitela, Stephen Baker, Raphael Rakotozandrindrainy & Florian Marks
Additional file 3. Mean ELISA ODs by antigen. Notes: OD=optical density; red dot=mean of original data; blue dot=mean of each 1,000 bootstrap random samples; light red dashed line=upper and lower 95% confidence interval (CI) of original data; dark red dashed line=upper and lower 99% confidence interval (CI) of original data

Registration Year

  • 2022
    31

Resource Types

  • Text
    18
  • Collection
    10
  • Dataset
    3

Affiliations

  • Kwame Nkrumah University of Science and Technology
    31
  • Kintampo Health Research Centre
    12
  • Kangbuk Samsung Hospital
    8
  • University of Cambridge
    8
  • Federal Ministry of Health
    8
  • World Health Organization
    8
  • University of Basel
    8
  • University of Antananarivo
    8
  • Heidelberg University
    8
  • Swiss Tropical and Public Health Institute
    8