642 Works

Host Factor Experiment (ICL012-R)

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Purpose: To obtain samples from Calu-3 cells infected with A/Vietnam/1203-CIP048_RG4/2004 (H5N1). Details: Time Points = 0, 3, 7, 12, 18 and 24hrs post infection were taken for mutant virus while wild type virus was only tested at 7 and 24hrs post-infection; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of samples;...

Host Factor Experiment (IM007-P)

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Purpose: To obtain lung samples from C57BL6 mice infected with Vietnam/1203-CIP048_RG4/2004 (H5N1) for both transcriptional and proteomic analyses. Details: Time Points = 1, 2, 4 and 7 days post infection; 5 replicates for infected mice and triplicate mice for the mocks; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^3 and 10^4 PFU.

Data for tree mortality calibration of satellite and LiDAR-derived fire severity estimates.

N. Macriss, T.J. Furniss, S.M.A. Jeronimo, E. L. Crowley, O. W. Germain, S. Germain, V. R. Kane, A. J. Larson & James A. Lutz
The 55 JFSP plots were established to capture forest types, fire histories, and severity levels beyond those found within the YFPD. The plots were square 50 m × 50 m plots established in Pinus ponderosa, Pinus jeffreyi, and Abies concolor – Pinus lambertiana forest types between 1,431 m and 2,250 m elevation. Plots were installed post-fire based on a randomly chosen locations stratified by burn severity levels as calculated from the differenced Normalized Burn Ratio...

Data for allometric equations of Chrysolepis sempervirens, Cornus sericea, Corylus cornuta ssp. californica, and Leucothoe davisiae.

James A. Lutz, J. A. Freund, A. J. Larson, M. E. Swanson, T. J. Furniss, K. M. L. Becker, E. M. Blomdahl, C. A. Cansier, S. J. Germain & S. M. A. Jeronimo
This data set includes measurements of 40 stems of Chrysolepis sempervirens (Kellogg) Hjelmq. (bush chinquapin), 41 stems of Cornus sericea L. (redosier dogwood), 50 stems of Corylus cornuta Marsh. ssp. californica (A. DC.) E. Murray, and 40 stems of Leucothoe davisiae Torrey (Sierra laurel), as reported in Lutz et al. (2014, 2017). Nomenclature follows Flora of North America (1993+).

Host Factor Experiment (SCL006-P)

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Purpose: To obtain samples for transcriptional analysis and proteomics in triplicate using wild typeicSARS urbani and icSARS Bat SRBD mutant in 2B4 cells/sorted Calu3 cells with high ACE2 expression. Overview of Experiment: Time Points = 0, 7, 12, 24, 30, 36, 48, 54, 60 and 72 hrs post infection. (Note: there is no time point at 7 h for icSARS Bat SRBD.) Done in triplicate for both RNA and Protein. Triplicates are defined as 3...

Host Factor Experiment (ICL004-R)

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Purpose: To obtain samples from Calu-3 cells infected with A/Vietnam/1203/2004(H5N1) for both transcriptional and proteomics analyses. Details: Time Points = 0, 3, 7, 12, 18, and 24 h post infection; Done in triplicate for both RNA and Protein; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of samples; Culture medium (the...

Chinese Funerary Biographies: Chinese Texts

Patricia Buckley Ebrey, Ping Yao & Cong Ellen Zhang
This text is an online supplement to Chinese Funerary Biographies: An Anthology of Remembered Lives, edited by Patricia Buckley Ebrey, Ping Yao, and Cong Ellen Zhang (University of Washington Press, 2019), containing the original biographies in Chinese.

Module 20: Early Numeracy [online module]

Host Factor Experiment (ICL010-P)

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Purpose: To obtain samples from Calu-3 cells infected with A/Netherlands/602/2009 (H1N1) and A/CA/04/09 (H1N1) for transcriptional analysis and proteomics analysis for A/Netherlands/602/2009. For A/Netherlands/602/2009 and mock infected the time points are 0, 3, 7, 12, 18, 24, 30, 36 and 48 h post infection. For A/CA/04/09 the time points are 0,12, 24 and 48 h post infection.There are triplicate samples for all conditions for both transcriptional and proteomics analyses. Triplicates are defined as 3 different...

Host Factor Experiment (ICL011-P)

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Purpose: To obtain samples from Calu-3 cells infected with mutants A/Vietnam/1203-CIP048_RG2/2004 (H5N1) and A/Vietnam/1203-CIP048_RG3/2004 (H5N1). Details: Time Points = 0, 3, 7, 12, 18 and 24hrs post infection were taken for mutant viruses while wild type virus was only tested at 7 and 24hrs post-infection; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock...

Host Factor Experiment (ICL012-P)

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Purpose: To obtain samples from Calu-3 cells infected with A/Vietnam/1203-CIP048_RG4/2004 (H5N1). Details: Time Points = 0, 3, 7, 12, 18 and 24hrs post infection were taken for mutant virus while wild type virus was only tested at 7 and 24hrs post-infection; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of samples;...

Host Factor Experiment (IM010-R)

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Purpose: To obtain lung samples from C57BL6 mice infected with Vietnam/1203/2004 (H5N1) for transcriptional analyses. Details: Mouse strains = WT, IDO1 knock-out and Tnfrsf1b knock-out; Time Points = 2 and 6 days post-infection; 2-3 replicates for each mouse strain; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 1000 pfu.

Host Factor Experiment (SM007-R)

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Purpose: To obtain lung samples from Tnfrsf1a/1b knock out mutant mice infected with SARS MA15 virus for transcriptional analyses. Details: Time Points = days 2, 4 and 7 post-infection; 2-4 replicate mice for each condition; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^4 pfu.

Host Factor Experiment (CA04M001-P)

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Purpose: To look at the host response to different doses across 4 time points after infection. Samples were collected for both transcriptomics and proteomics. General Design: 20 week-old C57Bl6 mice; Three Doses = 1E3, 1E4, 1E5, 1E6 (PFU); Time points of 1, 2, 4 and 7 days; ~5 mice/time point for infections; 3 mice/timepoint for time matched mocks

Data from: Does nasal echolocation influence the modularity of the mammal skull?

Sharlene E. Santana & Sarah E. Lofgren
In vertebrates, changes in cranial modularity can evolve rapidly in response to selection. However, mammals have apparently maintained their pattern of cranial integration throughout their evolutionary history and across tremendous morphological and ecological diversity. Here, we use phylogenetic, geometric morphometric and comparative analyses to test the hypothesis that the modularity of the mammalian skull has been remodelled in rhinolophid bats due to the novel and critical function of the nasal cavity in echolocation. We predicted...

Data from: Precipitation alters interactions in a grassland ecological community

Nicolas Deguines, Justin S. Brashares & Laura R. Prugh
Climate change is transforming precipitation regimes world-wide. Changes in precipitation regimes are known to have powerful effects on plant productivity, but the consequences of these shifts for the dynamics of ecological communities are poorly understood. This knowledge gap hinders our ability to anticipate and mitigate the impacts of climate change on biodiversity. Precipitation may affect fauna through direct effects on physiology, behaviour or demography, through plant-mediated indirect effects, or by modifying interactions among species. In...

Data from: Genetic signals of artificial and natural dispersal linked to colonization of South America by non-native Chinook salmon (Oncorhynchus tshawytscha)

Daniel Gomez-Uchida, Diego Cañas-Rojas, Carla M. Riva-Rossi, Javier E. Ciancio, Miguel A. Pascual, Billy Ernst, Eduardo Aedo, Selim S. Musleh, Francisca Valenzuela-Aguayo, Thomas P. Quinn, James E. Seeb & Lisa W. Seeb
Genetics data have provided unprecedented insights into evolutionary aspects of colonization by non-native populations. Yet, our understanding of how artificial (human-mediated) and natural dispersal pathways of non-native individuals influence genetic metrics, evolution of genetic structure, and admixture remains elusive. We capitalize on the widespread colonization of Chinook salmon Oncorhynchus tshawytscha in South America, mediated by both dispersal pathways, to address these issues using data from a panel of polymorphic SNPs. First, genetic diversity and the...

Data from: Rise and fall of a hybrid zone: implications for the roles of aggression, mate choice, and secondary succession

Travis R. Robbins, Lorelei E. Walker, Kelvin D. Gorospe, Stephen A. Karl, Aaron W. Schrey, Earl D. McCoy & Henry R. Mushinsky
Hybridization can be an important evolutionary force by generating new species and influencing evolution of parental species in multiple ways, including introgression and the consequences of hybrid vigor. Determining the ecological processes underlying evolution in hybrid zones is difficult however because it requires examining changes in both genotypic frequencies over time and corresponding ecological information, data that are rarely collected together. Here, we describe genetic and ecological aspects of a hybrid zone between the Eastern...

Data from: Hybridization between genetically modified Atlantic salmon and wild brown trout reveals novel ecological interactions

Krista B. Oke, Peter A. H. Westley, Darek T. R. Moreau & Ian A. Fleming
Interspecific hybridization is a route for transgenes from genetically modified (GM) animals to invade wild populations, yet the ecological effects and potential risks that may emerge from such hybridization are unknown. Through experimental crosses, we demonstrate transmission of a growth hormone transgene via hybridization between a candidate for commercial aquaculture production, GM Atlantic salmon (Salmo salar), and closely related wild brown trout (S. trutta). Transgenic hybrids were viable and grew more rapidly than transgenic salmon...

Data from: Individual marking of soft-bodied subtidal invertebrates in situ - a novel staining technique applied to the giant plumose anemone Metridium farcimen (Tilesius, 1809)

Christopher D. Wells & Kenneth P. Sebens
The ability to recognize individuals and track growth over time is crucial to population dynamics research as well as studies of animal behavior. Invertebrates are particularly difficult to track as they often molt, have regenerative capabilities, or lack hard parts to attach markers. We tested, in laboratory and field studies, a new way of marking sea anemones (order Actiniaria) by injection of three vital stains (i.e., neutral red, methylene blue, and fluorescein). Neutral red and...

Data from: Morphological and functional maturity of the oral jaws covary with offspring size in Trinidadian guppies

Terry R. Dial, Luz Patricia Hernandez & Elizabeth L. Brainerd
Large size of individual offspring is routinely selected for in highly competitive environments, such as in low-predation populations of the Trinidadian guppy (Poecilia reticulata). Large guppy offspring outcompete their smaller conspecifics, but the functional mechanisms underlying this advantage are unknown. We measured jaw kinematics during benthic feeding and cranial musculoskeletal morphologies in neonates and juveniles from five populations of Trinidadian guppy and found that both kinematics and morphologies vary substantially with neonatal size. Rotation at...

Data from: Spatial and temporal dynamics and value of nature-based recreation, estimated via social media

Laura J. Sonter, Keri B. Watson, Spencer A. Wood & Taylor H. Ricketts
Conserved lands provide multiple ecosystem services, including opportunities for nature-based recreation. Managing this service requires understanding the landscape attributes underpinning its provision, and how changes in land management affect its contribution to human wellbeing over time. However, evidence from both spatially explicit and temporally dynamic analyses is scarce, often due to data limitations. In this study, we investigated nature-based recreation within conserved lands in Vermont, USA. We used geotagged photographs uploaded to the photo-sharing website...

Data from: Go big or go fish: morphological specializations in carnivorous bats

Sharlene E. Santana & Elena Cheung
Specialized carnivory is relatively uncommon across mammals, and bats constitute one of the few groups in which this diet has evolved multiple times. While size and morphological adaptations for carnivory have been identified in other taxa, it is unclear what phenotypic traits characterize the relatively recent evolution of carnivory in bats. To address this gap, we apply geometric morphometric and phylogenetic comparative analyses to elucidate which characters are associated with ecological divergence of carnivorous bats...

Data from: A major shift in diversification rate helps explain macroevolutionary patterns in primate species diversity

Jessica H. Arbour & Sharlene E. Santana
Primates represent one of the most species rich, wide ranging and ecologically diverse clades of mammals. What major macroevolutionary factors have driven their diversification and contributed to the modern distribution of primate species remains widely debated. We employed phylogenetic comparative methods to examine the role of clade age and evolutionary rate heterogeneity in the modern distribution of species diversity of Primates. Primate diversification has accelerated since its origin, with decreased extinction leading to a shift...

Data from: Artificial selection on reproductive timing in hatchery salmon drives a phenological shift and potential maladaptation to climate change

Michael D. Tillotson, Heidy K. Barnett, Mary E. Buthimethee, Michele E. Koehler & Thomas P. Quinn
The timing of breeding migration and reproduction links generations and substantially influences individual fitness. In salmonid fishes, such phenological events (seasonal return to fresh water and spawning) vary among populations but are consistent among years, indicating local adaptation in these traits to prevailing environmental conditions. Changing reproductive phenology has been observed in many populations of salmonids, and is sometimes attributed to adaptive responses to climate change. The sockeye salmon spawning in the Cedar River near...

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