Additional file 1:

The experiment and results.

To investigate the molecular mechanism of the ADVENTITIOUS ROOTING RELATED OXYGENASE 1 (ARRO-1) gene in adventitious rooting of tree peony, the PsARRO-1 gene was isolated from Paeonia ostii. Subsequently, a yeast two-hybrid (Y2H) cDNA library using the split-ubiquitin yeast membrane system was constructed, the insert fragments lengths of pPR3-N membrane Y2H library were mainly concentrated between 500 and 3,000 bp, and the library capacity reached 1.3 × 107 CFUs, with an efficiency of recombinant of...

Osteoporosis is a worldwide disease that seriously affects the quality of life and survival rate of the elderly. The detection of bone biomarkers will provide supplementary information on bone mineral density, contributing to the accurate diagnosis of osteoporosis and better health care for prevention. This study aimed to investigate the efficacy of oxidative stress markers—8-oxo-7,8-dihydroguanine (8-oxoGsn) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGsn) in the assessment of osteoporosis. We conducted a cross-sectional study among menopausal women with a mean...

The aim of this study was to develop a gambogic acid-loaded mixed micelles (GA-M) system, using Kolliphor HS15 and lecithin, for enhancement of oral bioavailability. GA-M was prepared using the thin film hydration method, and particle size and zeta potential indexes were used to determine the optimized formulation was optimized with taking particle size, zeta potential as indexes. The optimal GA-M system had a mean particle size in the nanometer range (87.22 ± 0.68 nm)...

Additional file 1: Fig. S1. Time series and predicting epidemic magnitude of CVA16 activity in Anhua county during 2013-2016 (A) and that in Hunan province during 2009-2018 (B). Fig. S2. Participants’ included time in baseline and follow-up times (A) and the epidemic seasons of CVA16 activity during 2009-2018 in Anhua, Hunan province (B). Fig. S3. Distribution of maternal antibody titre transfer ratio against CVA16 for all pairs of neonate-mother (A) and positive mothers with antibody...

Additional file 1: Fig. S1. Time series and predicting epidemic magnitude of CVA16 activity in Anhua county during 2013-2016 (A) and that in Hunan province during 2009-2018 (B). Fig. S2. Participants’ included time in baseline and follow-up times (A) and the epidemic seasons of CVA16 activity during 2009-2018 in Anhua, Hunan province (B). Fig. S3. Distribution of maternal antibody titre transfer ratio against CVA16 for all pairs of neonate-mother (A) and positive mothers with antibody...

Supplementary Material 1

Supplemental Document

Abstract Background Gestational age (GA) is associated with later cognition and behavior. However, it is unclear how specific cognitive domains and brain structural development varies with the stepwise change of gestational duration. Methods This large-scale longitudinal cohort study analyzed 11,878 early adolescents’ brain volume maps at 9–10 years (baseline) and 5685 at 11–12 years (a 2-year follow-up) from the Adolescent Brain Cognitive Development (ABCD) study. According to gestational age, adolescents were divided into five categorical...

Abstract Background The shared care pathway for ADHD is a program developed in Canada with two main strategies: (a) implement a shared care pathway between general practitioners (GPs) and specialists, and (b) step up or down care so that the patient is treated at the most appropriate level of care, depending on the complexity or outcome of their illness. The current study aims to identify the challenges and facilitators of implementing this program in a...

Layered transition metal dichalcogenides (TMDCs) pave the way for fundamental research on atomically thin materials and optoelectronic devices. Due to their intrinsic 2D nature, few-layer TMDCs heterostructures exhibit several unique physical properties that are extremely sensitive to the number of layers. However, studies on the effects of interlayer coupling on excitons and valley polarization are still insufficient. Here, we investigate the polarization of the excitonics valley in MoS2-WS2 heterostructures using circular polarization-resolved photoluminescence. The valley...

Additional file 4: Table S1. Synthetic lethal interactive pairs and chemical inhibitors involving SWI/SNF members.

IntroductionBleeding after heart valve surgery is a serious clinical challenge. Hypocalcemia has been associated with the extent of bleeding in patients with spontaneous intracerebral hemorrhage. However, the association between blood calcium levels and bleeding extent in heart valve replacement patients has not been clearly established. This study aimed at determining the association between blood calcium levels and perioperative hemorrhage after heart valve replacement therapy.MethodsBased on preoperative blood calcium levels, patients were grouped into the hypocalcemia...

Abstract Background A major driver of cancer chromosomal instability is replication stress, the slowing or stalling of DNA replication. How replication stress and genomic instability are connected is not known. Aphidicolin-induced replication stress induces breakages at common fragile sites, but the exact causes of fragility are debated, and acute genomic consequences of replication stress are not fully explored. Results We characterize DNA copy number alterations (CNAs) in single, diploid non-transformed cells, caused by one cell...

Additional file 5. Table S1. List of primers required for this study.

Additional file 5: Fig. S1. MβCD plus anti-GD2 treatment inhibits tumor growth in vivo. A, Schematic of MβCD and anti-GD2 treatments. B, Representative images of xenograft 9464D tumors with different treatments. C, The tumors were removed for weight analysis 21 days after the beginning of treatments. D, Tumor growth curves of subcutaneous 9464D cells with different treatments. Fig. S2. Monitoring of mouse weight during the experiment.

Abstract Background Beta-1,3-galactosyltransferase-4 (B3GALT4) plays a critical regulatory role in tumor biology. However, the role of B3GALT4 in modulating the tumor microenvironment (TME) of neuroblastoma (NB) remains unknown. Methods Public datasets and clinical NB samples were collected to evaluate the expression and clinical significance of GD2 and B3GALT4 in NB patients. CCK-8, colony formation, and transwell assays and experiments in tumor-bearing mouse models were conducted to investigate the function of B3GALT4. Flow cytometry, ELISA, immunohistochemistry,...

Additional file 10: Table S5. The summary of lncRNAs exhibiting allele-specific expression in cultivated sunflower lines for edible fruit and oil identified in 12 DAP sunflower endosperm.

Additional file 12: Table S7. GO Gene Ontology analysis of co-expressed protein-coding genes with lncRNAs of allelic bias toward cultivated lines for oil.

Additional file 2: Table S2. The genomic information of lncRNAs in sunflower endosperm at 12DAP.

Additional file 11: Table S6. GO Gene Ontology analysis of co-expressed protein-coding genes with lncRNAs of allelic bias toward cultivated lines for edible fruit.

Background:Non-small-cell lung cancer (NSCLC) with additional nodule(s) located in the same lobe or ipsilateral different lobe were designated as T3 and T4, respectively, which was merely defined by anatomical location of additional nodule(s), regardless of other prognostic factors.Methods:A total of 4711 patients with T1-4, N0-2, M0 NSCLC undergoing complete resection were identified between 2009 and 2014, including 145 patients with additional nodule(s) in the same lobe (T3-Add) and 174 patients with additional tumor nodule(s) in...

Additional file 4: Figure S3. Volcano plot showing the log2 fold change of 517 significantly differentially expressed miRNAs in CRC patients from the TCGA database.

Additional file 5: Figure S4. Prediction of 6 miRNAs (miR-29b-3p, miR-4677-3p, miR-144-3p, miR-30b-5p, miR-30e-5p and miR-374b-5p) targeting LIFR-AS1 in CRC.