Additional file 1: Table S1. Association of baseline variables and comorbidities with levels of SARS-CoV-2 antibodies at time point 4 (January-February 2021) prior to the massive rollout of vaccination. Table S2. Seropositivity against the SARS-CoV-2 receptor-binding domain antigen from variants of concern. Table S3. Reinfections. Table S4. Significant associations of baseline variables with differential antibody rate of change. Fig. S1. Overall distribution of antibody responses for each isotype and antigen pair of the 128 pre-pandemic...

Additional file 1: Table S1. Association of baseline variables and comorbidities with levels of SARS-CoV-2 antibodies at time point 4 (January-February 2021) prior to the massive rollout of vaccination. Table S2. Seropositivity against the SARS-CoV-2 receptor-binding domain antigen from variants of concern. Table S3. Reinfections. Table S4. Significant associations of baseline variables with differential antibody rate of change. Fig. S1. Overall distribution of antibody responses for each isotype and antigen pair of the 128 pre-pandemic...

Abstract This study evaluated the persistence of IgM, IgA, and IgG to SARS-CoV-2 spike and nucleocapsid antigens up to 616 days since the onset of symptoms in a longitudinal cohort of 247 primary health care workers from Barcelona, Spain, followed up since the start of the pandemic. The study also assesses factors affecting antibody levels, including comorbidities and the responses to variants of concern as well as the frequency of reinfections. Despite a gradual and...

Additional file 3: Figure S3. Odds ratio for the association between mortality (1A–C), intensive care unit admission (2A–C) and peripheral artery disease in each of the four cohorts and overall. I2 indicates heterogeneity in the estimates. Odds ratio estimates adjusted for: model 1: age and sex (B); model 2: model 1 + type of diabetes, arterial hypertension, and the presence of microvascular disease (C).

Abstract Objective The purpose of this study was to examine the levels of cerebrospinal fluid (CSF) apolipoprotein E (apoE) species in Alzheimer’s disease (AD) patients. Methods We analyzed two CSF cohorts of AD and control individuals expressing different APOE genotypes. Moreover, CSF samples from the TgF344-AD rat model were included. Samples were run in native- and SDS-PAGE under reducing or non-reducing conditions (with or without β-mercaptoethanol). Immunoprecipitation combined with mass spectrometry or western blotting analyses...

Additional file 1. Images of complete blots from which figures were obtained, and also the boxes selected with the ImageQuant Studio software for quantification.

Additional file 1: Fig S1. Comparison of reference assemblies. Fig S2. Circos plots representing structural variation among strains. Fig S3. Dotplot representing improvements in assembly of strain CAS08-0016. Fig S4. Barplot representing origin of accessory families. Fig S5. Graphical representation of the adhesin detection pipeline. Fig S6. Gains and losses of adhesin families in the C. glabrata strains. Fig S7. Summary of the LongHam pipeline. Fig S8. Example of an adhesin gene cluster. Fig S9....

Additional file 2: Table S1. Assembly statistics for the re-assembly of the genomes from Arastehfar et al. [20]. Table S2. List of rearrangements when compared to the C. glabrata sanger reference strain. Table S3. Summary of the results of the gene annotation for the 21 C. glabrata strains. Table S4. Complete list of orthogroups as determined by orthofinder. Table S5. List of chromosome substitutions during assembly. Table S6. List of predicted adhesins for the 21...

Additional file 1: Figure S1. Odds ratio for the association between mortality (1A–C), intensive care unit admission (2A–C) and use of invasive mechanical ventilation during the hospitalization (3A–C) and ischemic heart disease in each of the four cohorts and overall. I2 indicates heterogeneity in the estimates. Odds ratio estimates adjusted for: model 1: age and sex (B); model 2: model 1 + type of diabetes, arterial hypertension, and the presence of microvascular disease (C).

Additional file 2: Figure S2. Odds ratio for the association between mortality (1A–C), intensive care unit admission (2A–C) and and stroke in each of the four cohorts and overall. I2 indicates heterogeneity in the estimates. Odds ratio estimates adjusted for: model 1: age and sex (B); model 2: model 1 + type of diabetes, arterial hypertension, and the presence of microvascular disease (C).

Abstract Background It is not clear whether pre-existing macrovascular complications (ischemic heart disease, stroke or peripheral artery disease) are associated with health outcomes in people with diabetes mellitus hospitalized for COVID-19. Methods We conducted cohort studies of adults with pre-existing diabetes hospitalized for COVID-19 infection in the UK, France, and Spain during the early phase of the pandemic (between March 2020—October 2020). Logistic regression models adjusted for demographic factors and other comorbidities were used to...

Additional file 3: Figure S3. Odds ratio for the association between mortality (1A–C), intensive care unit admission (2A–C) and peripheral artery disease in each of the four cohorts and overall. I2 indicates heterogeneity in the estimates. Odds ratio estimates adjusted for: model 1: age and sex (B); model 2: model 1 + type of diabetes, arterial hypertension, and the presence of microvascular disease (C).

Additional file 1: Fig S1. Comparison of reference assemblies. Fig S2. Circos plots representing structural variation among strains. Fig S3. Dotplot representing improvements in assembly of strain CAS08-0016. Fig S4. Barplot representing origin of accessory families. Fig S5. Graphical representation of the adhesin detection pipeline. Fig S6. Gains and losses of adhesin families in the C. glabrata strains. Fig S7. Summary of the LongHam pipeline. Fig S8. Example of an adhesin gene cluster. Fig S9....

Abstract Objective The purpose of this study was to examine the levels of cerebrospinal fluid (CSF) apolipoprotein E (apoE) species in Alzheimer’s disease (AD) patients. Methods We analyzed two CSF cohorts of AD and control individuals expressing different APOE genotypes. Moreover, CSF samples from the TgF344-AD rat model were included. Samples were run in native- and SDS-PAGE under reducing or non-reducing conditions (with or without β-mercaptoethanol). Immunoprecipitation combined with mass spectrometry or western blotting analyses...

Additional file 1. Images of complete blots from which figures were obtained, and also the boxes selected with the ImageQuant Studio software for quantification.

Additional file 3. Chromosome-level assemblies from diverse clades reveal limited structural and gene content variation in the genome of Candida glabrata.

Additional file 3. Chromosome-level assemblies from diverse clades reveal limited structural and gene content variation in the genome of Candida glabrata.

Abstract Background Candida glabrata is an opportunistic yeast pathogen thought to have a large genetic and phenotypic diversity and a highly plastic genome. However, the lack of chromosome-level genome assemblies representing this diversity limits our ability to accurately establish how chromosomal structure and gene content vary across strains. Results Here, we expanded publicly available assemblies by using long-read sequencing technologies in twelve diverse strains, obtaining a final set of twenty-one chromosome-level genomes spanning the known...

Additional file 1: Figure S1. Odds ratio for the association between mortality (1A–C), intensive care unit admission (2A–C) and use of invasive mechanical ventilation during the hospitalization (3A–C) and ischemic heart disease in each of the four cohorts and overall. I2 indicates heterogeneity in the estimates. Odds ratio estimates adjusted for: model 1: age and sex (B); model 2: model 1 + type of diabetes, arterial hypertension, and the presence of microvascular disease (C).

Additional file 2: Figure S2. Odds ratio for the association between mortality (1A–C), intensive care unit admission (2A–C) and and stroke in each of the four cohorts and overall. I2 indicates heterogeneity in the estimates. Odds ratio estimates adjusted for: model 1: age and sex (B); model 2: model 1 + type of diabetes, arterial hypertension, and the presence of microvascular disease (C).

Abstract Background It is not clear whether pre-existing macrovascular complications (ischemic heart disease, stroke or peripheral artery disease) are associated with health outcomes in people with diabetes mellitus hospitalized for COVID-19. Methods We conducted cohort studies of adults with pre-existing diabetes hospitalized for COVID-19 infection in the UK, France, and Spain during the early phase of the pandemic (between March 2020—October 2020). Logistic regression models adjusted for demographic factors and other comorbidities were used to...

Abstract Background Candida glabrata is an opportunistic yeast pathogen thought to have a large genetic and phenotypic diversity and a highly plastic genome. However, the lack of chromosome-level genome assemblies representing this diversity limits our ability to accurately establish how chromosomal structure and gene content vary across strains. Results Here, we expanded publicly available assemblies by using long-read sequencing technologies in twelve diverse strains, obtaining a final set of twenty-one chromosome-level genomes spanning the known...

Abstract This study evaluated the persistence of IgM, IgA, and IgG to SARS-CoV-2 spike and nucleocapsid antigens up to 616 days since the onset of symptoms in a longitudinal cohort of 247 primary health care workers from Barcelona, Spain, followed up since the start of the pandemic. The study also assesses factors affecting antibody levels, including comorbidities and the responses to variants of concern as well as the frequency of reinfections. Despite a gradual and...

Additional file 2: Table S1. Assembly statistics for the re-assembly of the genomes from Arastehfar et al. [20]. Table S2. List of rearrangements when compared to the C. glabrata sanger reference strain. Table S3. Summary of the results of the gene annotation for the 21 C. glabrata strains. Table S4. Complete list of orthogroups as determined by orthofinder. Table S5. List of chromosome substitutions during assembly. Table S6. List of predicted adhesins for the 21...