375 Works

Additional file 6 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 6: Figure S2. miRNAs with similar expression patterns in crown and leaf tissues at the winter dormancy stage, spring green-up stage and jointing stage.

Additional file 7 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 7: Figure S3. miRNAs with different expression patterns in crown and leaf tissues at the winter dormancy stage, spring green-up stage and jointing stage.

The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia

Ying Zhou, Yu Tang, Pan Fu, Dongxing Tian, Lianhua Yu, Yunkun Huang, Gang Li, Meng Li, Yong Wang, Zehua Yang, Xiaogang Xu, Zhe Yin, Dongsheng Zhou, Laurent Poirel & Xiaofei Jiang
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and blaKPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of blaKPC-IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and blaKPC-IncF plasmids...

The effects of different parity and delivery mode on wheezing disorders in the children—a retrospective cohort study in Fujian, China

Haiyan Gao, Chong Miao, Haibo Li, Meng Bai, Huijie Zhang, Zhengqin Wu, Wei Li, Wenjuan Liu, Libo Xu, Guanghua Liu & Yibing Zhu
The relationship between childbirth delivery methods and the risk of wheezing in children remains controversial. Few studies have explored it under different maternal conditions. To explore the influence of childbirth delivery method on the onset of wheezing in children of different parity. A total of 21716 patients were included in this retrospective observational study. Multivariable logistic regression was used to analyze the relationship between childbirth delivery method and wheezing in children under 18 years of...

Block-Wise Variable Selection for Clustering Via Latent States of Mixture Models

Beomseok Seo, Lin Lin & Jia Li
Mixture modeling is a major paradigm for clustering in statistics. In this article, we develop a new block-wise variable selection method for clustering by exploiting the latent states of the hidden Markov model on variable blocks or the Gaussian mixture model. The variable blocks are formed by depth-first-search on a dendrogram created based on the mutual information between any pair of variables. It is demonstrated that the latent states of the variable blocks together with...

Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Abstract Background Winter wheat requires prolonged exposure to low temperature to initiate flowering (vernalization). Shoot apical meristem of the crown is the site of cold perception, which produces leaf primordia during vegetative growth before developing into floral primordia at the initiation of the reproductive phase. Although many essential genes for winter wheat cold acclimation and floral initiation have been revealed, the importance of microRNA (miRNA) meditated post-transcriptional regulation in crowns is not well understood. To...

Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Abstract Background Winter wheat requires prolonged exposure to low temperature to initiate flowering (vernalization). Shoot apical meristem of the crown is the site of cold perception, which produces leaf primordia during vegetative growth before developing into floral primordia at the initiation of the reproductive phase. Although many essential genes for winter wheat cold acclimation and floral initiation have been revealed, the importance of microRNA (miRNA) meditated post-transcriptional regulation in crowns is not well understood. To...

A new dihydroflavone and a new polyacetylene glucoside from Bidens parviflora

Fu-Jie Cai, Chuan-Hou Li, Xiao-Hui Sun, Lu Wang, Jin-Li Tian, Wei Zhao, De-Gang Kong, Qing Liu & Hong-Lei Zhou
A new dihydroflavone, 2(S)-isookanin-4′-methoxy-8-O-β-D-glucopyranoside (1), and a new polyacetylene glucoside, (10S)-tridecane-2E-ene-4,6,8-triyne-1-ol-10-O-β-D-glucopyranoside (2), along with seven known compounds (3–9), were isolated from the herb of Bidens parviflora Willd. The structures of all the extracted compounds were elucidated by HR-ESI-MS, 1 D and 2 D NMR spectra, as well as circular dichroism (CD).

Comparative analysis of circular RNA enrichment methods

Huajuan Shi, Ying Zhou, Erteng Jia, Zhiyu Liu, Min Pan, Yunfei Bai, Xiangwei Zhao & Qinyu Ge
The circRNAs sequencing results vary due to the different enrichment methods and their performance is needed to systematic comparison. This study investigated the effects of different circRNA enrichment methods on sequencing results, including abundance and species of circRNAs, as well as the sensitivity and precision. This experiment was carried out by following four common circRNA enrichment methods: including ribosomal RNA depletion (rRNA–), polyadenylation and poly (A+) RNA depletion followed by RNase R treatment (polyA+RNase R),...

Efficacy and safety of pembrolizumab for treating advanced non-small-cell lung cancer: a meta-analysis of phase II and III randomized controlled trials

Tianyao Gong, Lei Liu, Yufei Liu, Ke Liu, Yang Yang, Liuying Li & Chuantao Zhang
We conducted a meta-analysis to systematically review the efficacy and safety of pembrolizumab for advanced NSCLC. Databases were searched for randomized controlled trials (RCTs) treated with pembrolizumab till July 2021. Seven RCTs and 3988 patients were included. Our analysis suggests that pembrolizumab was more effective at improving PFS (HR, 0.59; 95% CI: 0.43–0.79; p = 0.0005), OS (HR, 0.65; 95% CI: 0.55–0.76; p < 0.00001) and ORR (RR, 1.85; 95% CI: 1.64–2.09; p < 0.00001)...

The significant immune escape of pseudotyped SARS-CoV-2 variant Omicron

Li Zhang, Qianqian Li, Ziteng Liang, Tao Li, Shuo Liu, Qianqian Cui, Jianhui Nie, Qian Wu, Xiaowang Qu, Weijin Huang & Youchun Wang
The emergence of Omicron/BA.1 has brought new challenges to fight against SARS-CoV-2. A large number of mutations in the Spike protein suggest that its susceptibility to immune protection elicited by the existing COVID-19 infection and vaccines may be altered. In this study, we constructed the pseudotyped SARS-CoV-2 variant Omicron. The sensitivity of 28 serum samples from COVID-19 convalescent patients infected with SARS-CoV-2 original strain was tested against pseudotyped Omicron as well as the other variants...

Therapeutic effect of a novel curcumin derivative GT863 on a mouse model of amyotrophic lateral sclerosis

Hajime Kato, Hiroyasu Sato, Michiaki Okuda, Jun Wu, Shingo Koyama, Yasuhiko Izumi, Tomonori Waku, Mitsuyoshi Iino, Masashi Aoki, Shigeki Arawaka, Yasuyuki Ohta, Kenichi Ishizawa, Kanan Kawasaki, Yasuomi Urano, Tomohiro Miyasaka, Noriko Noguchi, Toshiaki Kume, Akinori Akaike, Hachiro Sugimoto & Takeo Kato
The present study investigated the therapeutic effects of the curcumin derivative 3-[(1E)-2-(1H-indol-6-yl)ethenyl]-5-[(1E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]ethenyl]-1H-pyrazole (GT863) in amyotrophic lateral sclerosis (ALS). The inhibitory effect of GT863 on superoxide dismutase 1 (SOD1) aggregation was evaluated in cell-free assays. GT863 interfered with the conformational changes of the SOD1 protein and later, oligomeric aggregation. Furthermore, its antioxidant, anti-inflammatory, and neuroprotective effects were evaluated in cell-free and cultured cell assays. GT863 inhibited H2O2− and glutamate-induced cytotoxicity and activated an antioxidant responsive element...

Additional file 2 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 2: Figure S1. Common and specific known miRNAs expressed in wheat crown tissues at four different growth and development stages.

Trypanosoma evansi evades host innate immunity by releasing extracellular vesicles to activate TLR2-AKT signaling pathway

Ran Wei, Xin Li, Xiaocen Wang, Nan Zhang, Yuru Wang, Xichen Zhang, Pengtao Gong & Jianhua Li
Surra, one of the most important animal diseases with economic consequences in Asia and South America, is caused by Trypanosoma evansi. However, the mechanism of immune evasion by T. evansi has not been extensively studied. In the present study, T. evansi extracellular vesicles (TeEVs) were characterized and the role of TeEVs in T. evansi infection were examined. The results showed that T. evansi and TeEVs could activate TLR2-AKT pathway to inhibit the secretions of IL-12p40,...

Metabolism-related long non-coding RNAs (lncRNAs) as potential biomarkers for predicting risk of recurrence in breast cancer patients

Jian-ying Ma, Shao-hua Liu, Jie Chen & Qin Liu
Metabolism affects the development, progression, and prognosis of various cancers, including breast cancer (BC). Our aim was to develop a metabolism-related long non-coding RNA (lncRNA) signature to assess the prognosis of BC patients in order to optimize treatment. Metabolism-related genes between breast tumors and normal tissues were screened out, and Pearson correlation analysis was used to investigate metabolism-related lncRNAs. In total, five metabolism-related lncRNAs were enrolled to establish prognostic signatures. Kaplan-Meier plots and the receiver...

Prognostic and immunological significance of metastasis associated lung adenocarcinoma transcript 1 among different kinds of cancers

Lili Guo, Xiuwen Zhang, Hongli Pan, Yang Li, Jing Wang, Lin Li, Yafang Dong, Xinxin Du, Jun Chen & Fengjie Guo
LncRNAs belong to the type of noncoding RNA transcripts, which exceed 200 nucleotides in size. MALAT1 as one of the earlier identified lncRNAs in cancer is investigated by more and more scientific researchers. Expression, clinical significance and function of MALAT1 in pan-cancer exist as big difference. To detect the expression and clinical significance of MALAT1 gene precisely and comprehensively among different kinds of cancers, some classical databases such as GEPIA, TIMER, KM Plotter, and PrognoScan...

sj-pdf-1-prx-10.1177_00332941211029621 - Supplemental material for Working College Students’ Time Pressure and Work-School Conflict: Do Boundary Permeability and Dispositional Mindfulness Matter?

Min (Maggie) Wan, Li Feng, Xiao Meng, Muxin Zhai & Robert Konopaske
Supplemental material, sj-pdf-1-prx-10.1177_00332941211029621 for Working College Students’ Time Pressure and Work-School Conflict: Do Boundary Permeability and Dispositional Mindfulness Matter? by Min (Maggie) Wan, Li Feng, Xiao Meng, Muxin Zhai and Robert Konopaske in Psychological Reports

Working College Students’ Time Pressure and Work-School Conflict: Do Boundary Permeability and Dispositional Mindfulness Matter?

Min (Maggie) Wan, Li Feng, Xiao Meng, Muxin Zhai & Robert Konopaske
Although working college students are experiencing increasing demands on their time, the influence of time pressure on students’ work-school experience has been under-studied in the extant career development literature. Drawing on boundary theory and conservation of resources theory, the present research investigates the degree to which work or school time pressure is associated with working college students’ work-school conflict through work-school boundary permeability. Moreover, this study considers dispositional mindfulness as an individual resource that buffers...

Tumour suppressor TET2 safeguards enhancers from aberrant DNA methylation and epigenetic reprogramming in ERα-positive breast cancer cells

Ruitu Lyu, Xuguo Zhu, Yinghui Shen, Lijun Xiong, Lu Liu, Hang Liu, Feizhen Wu, Christian Argueta & Li Tan
Aberrant DNA methylation is an epigenetic hallmark of malignant tumours. The DNA methylation level is regulated by not only DNA methyltransferases (DNMTs) but also Ten-Eleven Translocation (TET) family proteins. However, the exact role of TET genes in breast cancer remains controversial. Here, we uncover that the ERα-positive breast cancer patients with high TET2 mRNA expression had better overall survival rates. Consistently, knockout of TET2 promotes the tumorigenesis of ERα-positive MCF7 breast cancer cells. Mechanistically, TET2...

Tumor suppressor TET2 safeguards enhancers from aberrant DNA methylation and epigenetic reprogramming in ERα-positive breast cancer cells

Ruitu Lyu, Xuguo Zhu, Yinghui Shen, Lijun Xiong, Lu Liu, Hang Liu, Feizhen Wu, Christian Argueta & Li Tan
Aberrant DNA methylation is an epigenetic hallmark of malignant tumors. The DNA methylation level is regulated by not only DNA methyltransferases (DNMTs) but also Ten-Eleven Translocation (TET) family proteins. However, the exact role of TET genes in breast cancer remains controversial. Here, we uncover that the ERα-positive breast cancer patients with high TET2 mRNA expression had better overall survival rates. Consistently, knockout of TET2 promotes the tumorigenesis of ERα-positive MCF7 breast cancer cells. Mechanistically, TET2...

Tumor-associated neutrophils activated by tumor-derived CCL20 (C-C motif chemokine ligand 20) promote T cell immunosuppression via programmed death-ligand 1 (PD-L1) in breast cancer

Louis Boafo Kwantwi, Shujing Wang, Wenjun Zhang, Weidong Peng, Zeyu Cai, Youjing Sheng, Han Xiao, Xian Wang & Qiang Wu
Breast cancer is the leading cause of cancer-related death among women despite the significant improvement in diagnosis and treatment. Tumor-associated neutrophils have been shown to suppress antitumor functions of the host. However, how breast cancer tumor microenvironment influences the phenotype and functions of neutrophils to potentiate T cell immunosuppression is unknown. Herein, neutrophils isolated from peripheral blood of healthy donors were treated with supernatants from breast cancer cell lines or recombinant human CCL20. PD-L1 expression...

Synthesis of N-doped carbon dots for highly selective and sensitive detection of metronidazole in real samples and its cytotoxicity studies

Xiaoxiang Wang, Tao Lin, Wei Wu, Haisuo Wu & Dongdong Yan
The current investigation reports the synthesis of N-CDs using glucosamine, ascorbic acid, and ethylenediamine precursors by a simple hydrothermal technique. The formation of N-CDs was proved by various characterisation techniques such as X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Fourier-Transform Infrared Spectrophotometer (FT-IR). The optical properties were investigated by fluorescence and UV-vis spectrophotometer. Also, N-CDs showed high selectivity in detecting the MTZ compared to several other analytes. However, the metronidazole...

Silencing of let-7b-5p inhibits ovarian cancer cell proliferation and stemness characteristics by Asp-Glu-Ala-Asp-box helicase 19A

Xiujuan Huang, Hongxia Dong, Yang Liu, Fen Yu, Shunshi Yang, Zhen Chen & Jueying Li
The emergence and recurrence of ovarian cancer are associated with ovarian cancer stem cells. For cancer treatment, gene delivery of microbubbles (MB)-mediated microRNA (miRNA) is considered as a promising approach. In this study, our aim is to investigate the effects of MB-mediated let-7b-5p inhibitor on the proliferation and stemness characteristics of ovarian cancer (OVCA) cells. Let-7b-5p inhibitor mediated by MB was prepared (termed MB-let-7b-5p inhibitor), and the effects of MB-let-7b-5p inhibitor and let-7b-5p inhibitor on...

Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF

Ying Xu, Wen Xu, Wei Liu, Gaofeng Chen, Shili Jiang, Jiamei Chen, Xun Jian, Hua Zhang, Ping Liu & Yongping Mu
Our previous studies indicated that Yiguanjian decoction (YGJ) has an anti-hepatic-fibrosis effect and could regulate macrophage status. To elucidate the mechanism of YGJ in regulating macrophages. Liver cirrhosis was induced by CCl4 for 12 weeks combined with 2-acetylaminofluorene (2-AAF) for the last 4 weeks in male Wistar rats. YGJ (3.56 mg/kg) orally administered in the last 4 weeks, and SORA (1 mg/kg) as control. In vitro, RAW264.7 cells were treated with lipopolysaccharides (LPSs) to induce...

Establishment of a venovenous extracorporeal membrane oxygenation in a rat model of acute respiratory distress syndrome

Yongnan Li, Jian Huang, Rongzhi Zhang, Shixiong Wang, Xingdong Cheng, Pengbin Zhang, Kerong Zhai, Wei Wang, Debin Liu & Bingren Gao
Introduction:Venovenous extracorporeal membrane oxygenation (VV ECMO) is now considered a reasonable option to salvage acute respiratory distress syndrome (ARDS). However, we lack a rodent model for experimental studies. This study was undertaken to establish an animal model of VV ECMO in ARDS rats.Methods:A total of 18 Sprague-Dawley (SD) rats (350 ± 50 g) were used in this study. Using a rat model of oleic acid (OA)-induced ARDS, VV ECMO was established through cavoatrial cannulation of...

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