108 Works

NOX5-induced uncoupling of endothelial NO synthase is a causal mechanism and theragnostic target of an age-related hypertension endotype

Harald H. H. W. Schmidt, Mahmoud H. Elbatreek, Sepideh Sadegh, Elisa Anastasi, Emre Guney, Cristian Nogales, Tim Kacprowski, Ahmed A. Hassan, Andreas Teubner, Po-Hsun Huang, Chien-Yi Hsu, Paul M. H. Schiffers, Ger M. Janssen, Pamela W. M. Kleikers, Anil Wipat, Jan Baumbach & Jo G. R. De Mey
Hypertension is the most important cause of death and disability in the elderly. In 9 out of 10 cases, the molecular cause, however, is unknown. One mechanistic hypothesis involves impaired endothelium-dependent vasodilation through reactive oxygen species (ROS) formation. Indeed, ROS forming NADPH oxidase (Nox) genes associate with hypertension, yet target validation has been negative. We re-investigate this association by molecular network analysis and identify NOX5, not present in rodents, as a sole neighbor to human...

Correlative 3D SBFSEM data from: Intermittent bulk release of human cytomegalovirus

Felix Flomm, Timothy K Soh, Carola Schneider, Linda Wedemann, Hannah M Britt, Konstantinos Thalassinos, Soeren Pfitzner, Rudolph Reimer, Kay Grünewald & Jens Bernhard Bosse
Human Cytomegalovirus (HCMV) can infect a variety of cell types by using virions of varying glycoprotein compositions. It is still unclear how this diversity is generated, but spatio-temporally separated envelopment and egress pathways might play a role. So far, one egress pathway has been described in which HCMV particles are individually enveloped into small vesicles and are subsequently exocytosed continuously. However, some studies have also found enveloped virus particles inside multivesicular structures but could not...

Anti-EGFR-Based Therapy in Recurrent or Metastatic HNSCC – What Difference Does it Make?

Hendrik Eggers, Lea Häbel, Arnold Ganser, Viktor Grünwald, Roland Merten, Athanasia Warnecke, Martin Durisin & Philipp Ivanyi
Patients with R/M HNSCC treated with palliative first-line therapy at Hannover Medical School between October 2005 and December 2016 have been included to show changes in survival following broad utilization of cetuximab. Treatment periods were defined from 10/2005 to 12/2008 (Period A) and 01/2009 to 12/2016. Overall survival did not improve over time. However, in subgroup analysis cetuximab utilized at any time vs. never showed a significant improve of overall survival (11.3 vs. 6.3 months,...

Fecal microbiota dynamics and its relationship to diarrhea and health in dairy calves

Hongwei Chen, Yalu Liu, Kailang Huang, Bin Yang, Yuanyuan Zhang, Zhongtang Yu & Jiakun Wang
Abstract Background Diarrhea is a major cause of morbidity and mortality in young calves, resulting in considerable economic loss for dairy farms. To determine if some gut microbes might have resistance to dysbiotic process with calf diarrhea by dictating the microbial co-occurrence patterns from birth to post-weaning, we examined the dynamic development of the gut microbiota and diarrhea status using two animal trials, with the first trial having 14 Holstein dairy calves whose fecal samples...

Associations of habitual glucosamine supplementation with incident gout: a large population based cohort study

Mengyi Liu, Ziliang Ye, Yanjun Zhang, Sisi Yang, Qimeng Wu, Chun Zhou, Panpan He, Yuanyuan Zhang, Xiaoqin Gan & Xianhui Qin
Abstract Objectives The association between habitual glucosamine use and incident gout has not been examined in previous studies. We aimed to evaluate the association of habitual use of glucosamine with the risk of gout in general population. Methods A total of 436,594 participants (55.4% female) without prior gout at baseline who completed a questionnaire on supplementation use, which included glucosamine, in the UK Biobank were enrolled. Incident gout was recorded from self-report, death register, primary...

Additional file 1 of Accuracy of intraoral real-time navigation versus static, CAD/CAM-manufactured pilot drilling guides in dental implant surgery: an in vitro study

Robert Stünkel, Alexander-Nicolai Zeller, Thomas Bohne, Florian Böhrnsen, Edris Wedi, David Raschke & Philipp Kauffmann
Additional file 1: Fig. S1. A) Schematic illustration of spatial offsets from implant planning. Analysis shows deviations in drilling depth, oro-vestibular as well as mesio-distal direction and angulation. B) Section of the Treatment Evaluation Tool in coDiagnostix®. Planning is shown in blue. Red shows the actual implant position. C) Mandible model used for the in vitro testing with pilot drilling guide in situ. D) DENACAM system used for the in vitro testing. E) Major findings...

Inhaled mosliciguat (BAY 1237592): targeting pulmonary vasculature via activating apo-sGC

Eva M. Becker-Pelster, Michael G. Hahn, Martina Delbeck, Lisa Dietz, Jörg Hüser, Johannes Kopf, Thomas Kraemer, Tobias Marquardt, Thomas Mondritzki, Johannes Nagelschmitz, Sylvia M. Nikkho, Philippe V. Pires, Hanna Tinel, Gerrit Weimann, Frank Wunder, Peter Sandner, Joachim Schuhmacher, Johannes-Peter Stasch & Hubert K. F. Truebel
Abstract Background Oxidative stress associated with severe cardiopulmonary diseases leads to impairment in the nitric oxide/soluble guanylate cyclase signaling pathway, shifting native soluble guanylate cyclase toward heme-free apo-soluble guanylate cyclase. Here we describe a new inhaled soluble guanylate cyclase activator to target apo-soluble guanylate cyclase and outline its therapeutic potential. Methods We aimed to generate a novel soluble guanylate cyclase activator, specifically designed for local inhaled application in the lung. We report the discovery and...

Do in-service training materials for midwifery care providers in sub-Saharan Africa meet international competency standards? A scoping review 2000–2020

Joanne Welsh, Hashim Hounkpatin, Mechthild M. Gross, Claudia Hanson & Ann-Beth Moller
Abstract Background Levels of maternal and neonatal mortality remain high in sub-Saharan Africa, with an estimated 66% of global maternal deaths occurring in this region. Many deaths are linked to poor quality of care, which in turn has been linked to gaps in pre-service training programmes for midwifery care providers. In-service training packages have been developed and implemented across sub-Saharan Africa in an attempt to overcome the shortfalls in pre-service training. This scoping review has...

Additional file 2 of Do in-service training materials for midwifery care providers in sub-Saharan Africa meet international competency standards? A scoping review 2000–2020

Joanne Welsh, Hashim Hounkpatin, Mechthild M. Gross, Claudia Hanson & Ann-Beth Moller
Supplementary Material 2

Additional file 5 of Fecal microbiota dynamics and its relationship to diarrhea and health in dairy calves

Hongwei Chen, Yalu Liu, Kailang Huang, Bin Yang, Yuanyuan Zhang, Zhongtang Yu & Jiakun Wang
Additional file 5: Fig. S4. Heatmap of the ASVs that identified to be diarrheic status transition-associated in trial 2. The ASVs were identified based on fold change and analysis using LEfSe or DESeq2.

Autoimmune hepatitis in young Somalian men – experience from a German tertiary care center (Case reports)

Theresa Kirchner, Bjoern Hartleben, Sophia Koehler, Frank Schuppert, Irina Berger, Heiner Wedemeyer & Ingmar Mederacke
Histological changes in somalian patients with autoimmune hepatitis.

Additional file 11 of Epigenome-wide analysis of T-cell large granular lymphocytic leukemia identifies BCL11B as a potential biomarker

Patricia Johansson, Teresa Laguna, Julio Ossowski, Vera Pancaldi, Martina Brauser, Ulrich Dührsen, Lara Keuneke, Ana Queiros, Julia Richter, José I. Martín-Subero, Reiner Siebert, Brigitte Schlegelberger, Ralf Küppers, Jan Dürig, Eva M. Murga Penas, Enrique Carillo-de Santa Pau & Anke K. Bergmann
Additional file 11: Material and Methods.

Additional file 11 of Epigenome-wide analysis of T-cell large granular lymphocytic leukemia identifies BCL11B as a potential biomarker

Patricia Johansson, Teresa Laguna, Julio Ossowski, Vera Pancaldi, Martina Brauser, Ulrich Dührsen, Lara Keuneke, Ana Queiros, Julia Richter, José I. Martín-Subero, Reiner Siebert, Brigitte Schlegelberger, Ralf Küppers, Jan Dürig, Eva M. Murga Penas, Enrique Carillo-de Santa Pau & Anke K. Bergmann
Additional file 11: Material and Methods.

Additional file 1 of Partial otubain 1 deficiency compromises fetal well-being in allogeneic pregnancies despite no major changes in the dendritic cell and T cell compartment

Annika Stutz, Gopala Nishanth, Ana C. Zenclussen & Anne Schumacher
Supplementary Material 1. Representative flow cytometry dot plots are displayed for different DC and T cell populations within each organ analyzed. First, total lymphocytes were gated. Then, DC and T cell frequencies were determined according to their specific marker combinations

Functional proteomic profiling links deficient DNA clearance with increased mortality in individuals with severe COVID-19 pneumonia

Iker Valle Aramburu, Dennis Hoving, Spyros I Vernardis, Martha CF Tin, Marianna Ioannou, Mia I Temkin, Nathalia M De Vasconcelos, Vadim Demichev, Elisa Theresa Helbig, Lena Lippert, Klaus Stahl, Matthew White, Helena Radbruch, Jana Ihlow, David Horst, Scott T Chiesa, John E Deanfield, Sascha David, Christian Bode, Florian Kurth, Markus Ralser & Venizelos Papayannopoulos
The factors that influence survival during severe infection are unclear. Extracellular chromatin drives pathology, but the mechanisms enabling its accumulation remain elusive. Here, we show that in murine sepsis models, splenocyte death interferes with chromatin clearance through the release of the DNase I inhibitor actin. Actin-mediated inhibition was compensated by upregulation of DNase I or the actin scavenger gelsolin. Splenocyte death and neutrophil extracellular trap (NET) clearance deficiencies were prevalent in individuals with severe COVID-19...

Detection of differential selection pressure and functional-specific sites in subunits of vertebrate neuronal nicotinic acetylcholine receptors

Mengwen Zhao, Yuequn Ma, Juncai Xin, Changying Cao & Ju Wang
The nicotinic acetylcholine receptors (nAChR) are made of subunits evolved from a common ancestor. Despite the similarity in their sequences and structures, the properties of these subunits vary significantly. Thus, identifying the evolution features and function-related sites specific to each subunit is essential for understanding the characteristics of the subunits and the receptors assembled by them. In this study, we examined the sequence features of the nine neuronal nAChRs subunits from representative vertebrate species. Analysis...

Early clinical course of biopsy-proven IgA vasculitis nephritis

Sarina Butzer, Imke Hennies, Charlotte Gimpel, Jutta Gellermann, Gesa Schalk, Sabine König, Anja K. Büscher, Anja Lemke & Martin Pohl
Abstract Background IgA vasculitis (IgAV) is the most common form of systemic vasculitis in childhood and frequently involves the kidney. A minority of patients with IgA vasculitis nephritis (IgAVN), especially those presenting with heavy proteinuria and/or kidney failure at onset, are at risk of chronic end-stage kidney disease. For deciding upon treatment intensity, knowledge of the short-term clinical course of IgAVN is needed to improve treatment algorithms. Methods For this retrospective multicenter study, the medical...

Inhaled mosliciguat (BAY 1237592): targeting pulmonary vasculature via activating apo-sGC

Eva M. Becker-Pelster, Michael G. Hahn, Martina Delbeck, Lisa Dietz, Jörg Hüser, Johannes Kopf, Thomas Kraemer, Tobias Marquardt, Thomas Mondritzki, Johannes Nagelschmitz, Sylvia M. Nikkho, Philippe V. Pires, Hanna Tinel, Gerrit Weimann, Frank Wunder, Peter Sandner, Joachim Schuhmacher, Johannes-Peter Stasch & Hubert K. F. Truebel
Abstract Background Oxidative stress associated with severe cardiopulmonary diseases leads to impairment in the nitric oxide/soluble guanylate cyclase signaling pathway, shifting native soluble guanylate cyclase toward heme-free apo-soluble guanylate cyclase. Here we describe a new inhaled soluble guanylate cyclase activator to target apo-soluble guanylate cyclase and outline its therapeutic potential. Methods We aimed to generate a novel soluble guanylate cyclase activator, specifically designed for local inhaled application in the lung. We report the discovery and...

Additional file 1 of Inhaled mosliciguat (BAY 1237592): targeting pulmonary vasculature via activating apo-sGC

Eva M. Becker-Pelster, Michael G. Hahn, Martina Delbeck, Lisa Dietz, Jörg Hüser, Johannes Kopf, Thomas Kraemer, Tobias Marquardt, Thomas Mondritzki, Johannes Nagelschmitz, Sylvia M. Nikkho, Philippe V. Pires, Hanna Tinel, Gerrit Weimann, Frank Wunder, Peter Sandner, Joachim Schuhmacher, Johannes-Peter Stasch & Hubert K. F. Truebel
Additional file 1: Fig. S1. Treatment scheme of inhaled and systemic administered mosliciguat and of vehicle treatment in the minipig model. Each animal underwent eight unilateral ventilation cycles and animals were divided into two groups.

Development and validation of a RASS-related nomogram to predict the in-hospital mortality of neurocritical patients: a retrospective analysis based on the MIMIC-IV clinical database

Shenyan Gu, Yuqin Wang, Kaifu Ke, Xin Tong, Jiahui Gu & Yuanyuan Zhang
Richmond agitation-sedation scale (RASS) is a simple and widely used tool for evaluating sedation and agitation in adult ICU patients. Early deep sedation has been shown to be an important independent predictor of death, however, studies on the role of RASS in the prognostic assessment of neurocritical patients are lacking. The purpose of this study was to investigate the relationship between RASS and in-hospital mortality in neurocritical patients, and to develop and validate an effective...

Autoimmune hepatitis in young Somalian men – experience from a German tertiary care center (Case reports)

Theresa Kirchner, Bjoern Hartleben, Sophia Koehler, Frank Schuppert, Irina Berger, Heiner Wedemeyer & Ingmar Mederacke
Histological changes in somalian patients with autoimmune hepatitis.

Generation of functional fat organoid from rat superficial fascia

Yanfei Zhang, Yuanyuan Zhang, Yingyue Dong, Tongsheng Chen & Guoheng Xu
The organoid is a 3D cell architecture formed by self-organized tissues or cells in vitro with similar cell types, histological structures, and biological functions of the native organ. Depending on the unique organ structures and cell types, producing organoids requires individualized design and is still challenging. Organoids of some tissues, including adipose tissue, remain to generate to be more faithful to their original organ in structure and function. We previously established a new model of...

Design, synthesis and biological characteristics of pyrazolo[3,4-d]pyrimidine derivatives as potential VEGFR-2 inhibitors - Supporting Information

Dan-Xia Ying, Ju Wang, Xiu-Fang Li, Wen Zhang & Guo-Wu Rao
Supplementary: The 1H-NMR, 13C-NMR and MS data of synthesized compounds I-1 to 6. Supplementary 1: I-1 Supplementary: The 1H-NMR, 13C-NMR and MS data of synthesized compounds Ⅱ-1 to 6. Supplementary 7: II-1

Additional file 1 of Epigenome-wide analysis of T-cell large granular lymphocytic leukemia identifies BCL11B as a potential biomarker

Patricia Johansson, Teresa Laguna, Julio Ossowski, Vera Pancaldi, Martina Brauser, Ulrich Dührsen, Lara Keuneke, Ana Queiros, Julia Richter, José I. Martín-Subero, Reiner Siebert, Brigitte Schlegelberger, Ralf Küppers, Jan Dürig, Eva M. Murga Penas, Enrique Carillo-de Santa Pau & Anke K. Bergmann
Additional file 1: Table S1. T-LGL patient data. Table S2. Benign samples. Table S3. Sample overview. Table S4. Primer sequences and CpGs used in pyrosequencing. Table S5. CpGs validated by pyrosequencing. Table S6. Pearson correlation of results of bisulfite sequencing and methylation array data. Table S7. Chromatin states—description. Table S8. Taqman probes used in qPCR. Table S9. Differentially methylated probes and genomic features. Table S10. Differentially hypermethylated genes. Table S11. Differentially hypomethylated genes. Table S12....

Autoimmune hepatitis in young Somalian men – experience from a German tertiary care center (Case reports)

Theresa Kirchner, Bjoern Hartleben, Sophia Koehler, Frank Schuppert, Irina Berger, Heiner Wedemeyer & Ingmar Mederacke
Histological changes in somalian patients with autoimmune hepatitis.

Registration Year

  • 2022
    108

Resource Types

  • Text
    42
  • Image
    27
  • Collection
    26
  • Dataset
    13

Affiliations

  • Hannover Medical School
    108
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    30
  • Sichuan University
    28
  • Capital Medical University
    28
  • Xi'an Jiaotong University
    26
  • Nanfang Hospital
    26
  • Southern Medical University
    26
  • Sheng Jing Hospital
    26
  • Wake Forest University
    26
  • Chinese Academy of Sciences
    26