Additional file 1: Supplementary Figure S1. Genome coverage plot of B.intestinalis reported by Wochenende. Wochenende did not misclassify B. subtilis in the mock community by Sui et al (SRSRR11207337) [29], but rather detected B. intestinalis with a high and evenly distributed coverage. Indeed all the other tools also reported B. intestinalis instead of B. subtilis. Supplementary Figure S2. Wochenende analysis of an alternative long-read Zymo Even DNA mock community. The mock community was sequenced on...

Abstract Background Shotgun metagenome analysis provides a robust and verifiable method for comprehensive microbiome analysis of fungal, viral, archaeal and bacterial taxonomy, particularly with regard to visualization of read mapping location, normalization options, growth dynamics and functional gene repertoires. Current read classification tools use non-standard output formats, or do not fully show information on mapping location. As reference datasets are not perfect, portrayal of mapping information is critical for judging results effectively. Results Our alignment-based...

Human Cytomegalovirus (HCMV) can infect a variety of cell types by using virions of varying glycoprotein compositions. It is still unclear how this diversity is generated, but spatio-temporally separated envelopment and egress pathways might play a role. So far, one egress pathway has been described in which HCMV particles are individually enveloped into small vesicles and are subsequently exocytosed continuously. However, some studies have also found enveloped virus particles inside multivesicular structures but could not...

Additional file 1. Comparative subgroup analysis. Of 66 patients, we identified 28 children with crescentic glomeruli and/or nephrotic syndrome at onset of IgAVN. Referring to the German Society of Pediatric Nephology, a corticosteroid pulse therapy only would have been recommended. Of these 28 children, 17 received corticosteroid pulse therapy per protocol, 11 did not receive any immunosuppressive therapy. We therefore performed a comparative subgroup analysis which showed similar improvements of clinical symptoms and proteinuria.

Abstract Background Adipose tissue-derived adipokines are involved in various crosstalk between adipose tissue and other organs. Omentin1, a novel adipokine, exerts vital roles in the maintenance of body metabolism, insulin resistance and the like. However, the protective effect of omentin1 in myocardial ischemia (MI)-induced heart failure (HF) and its specific mechanism remains unclear and to be elucidated. Methods The model of MI-induced HF mice and oxygen glucose deprivation (OGD)-injured cardiomyocytes were performed. Mice with overexpression...

Additional file1: Figure S1. Representative immunohistochemical images of the expression of omentin1 in adipose tissues of mice injected with AAV-NC or AAV-omentin1, and the statistical results of staining intensity of omentin1 were presented, scale bar = 250 μm (n = 3). The experiments were performed in triplicate. Results were expressed as mean ± SD. ##P < 0.01, ####P < 0.0001 vs. the sham group; ****P < 0.0001 vs. the model group, one-way ANOVA. Figure S2....

Abstract Background Nowadays, 3D planning and static for dynamic aids play an increasing role in oral rehabilitation of the masticatory apparatus with dental implants. The aim of this study is to compare the accuracy of implant placement using a 3D-printed drilling guide and an intraoral real-time dynamic navigation system. Methods A total of 60 implants were placed on 12 partially edentulous lower jaw models. 30 were placed with pilot drilling guides, the other half with...

Additional file 1: Fig. S1. Treatment scheme of inhaled and systemic administered mosliciguat and of vehicle treatment in the minipig model. Each animal underwent eight unilateral ventilation cycles and animals were divided into two groups.

Additional file 3: Table S1. The ASVs in co-occurrence pattern of trial 1 stage 4.

Additional file 4: Fig. S3. Principal coordinates analysis (PCoA) plot of fecal microbiotas among ages in trial 2. The fecal microbiotas differences (P values) between diarrheic and non-diarrheic calves within the same age were compared.

Additional file 1: Figure S1. Flow chart of study participants. Figure S2. Association of glucosamine use and the risk of gout stratified by potential risk factors in males. Table S1. Single nucleotide polymorphisms used to build the genetic risk score for gout.

Additional file 1: Figure S1. Flow chart of study participants. Figure S2. Association of glucosamine use and the risk of gout stratified by potential risk factors in males. Table S1. Single nucleotide polymorphisms used to build the genetic risk score for gout.

Abstract Objectives The association between habitual glucosamine use and incident gout has not been examined in previous studies. We aimed to evaluate the association of habitual use of glucosamine with the risk of gout in general population. Methods A total of 436,594 participants (55.4% female) without prior gout at baseline who completed a questionnaire on supplementation use, which included glucosamine, in the UK Biobank were enrolled. Incident gout was recorded from self-report, death register, primary...

The factors that influence survival during severe infection are unclear. Extracellular chromatin drives pathology, but the mechanisms enabling its accumulation remain elusive. Here, we show that in murine sepsis models, splenocyte death interferes with chromatin clearance through the release of the DNase I inhibitor actin. Actin-mediated inhibition was compensated by upregulation of DNase I or the actin scavenger gelsolin. Splenocyte death and neutrophil extracellular trap (NET) clearance deficiencies were prevalent in individuals with severe COVID-19...

Supplemental material, sj-docx-1-opp-10.1177_10781552221144303 for Knowledge and practices about safe handling regarding the risk of exposure to antineoplastic drugs for caregivers in compounding units and in operating rooms performing HIPEC/PIPAC by Clémence Delafoy, Hubert Benoist, Alex Patin, Michèle Vasseur, Sonia Guillouet, Clarisse Eveno, Jean-Marc Guilloit, Pascal Odou, Nicolas Simon and Guillaume Saint-Lorant in Journal of Oncology Pharmacy Practice

Supplemental material, sj-docx-2-opp-10.1177_10781552221144303 for Knowledge and practices about safe handling regarding the risk of exposure to antineoplastic drugs for caregivers in compounding units and in operating rooms performing HIPEC/PIPAC by Clémence Delafoy, Hubert Benoist, Alex Patin, Michèle Vasseur, Sonia Guillouet, Clarisse Eveno, Jean-Marc Guilloit, Pascal Odou, Nicolas Simon and Guillaume Saint-Lorant in Journal of Oncology Pharmacy Practice

IntroductionEver since the late 1970s, occupational exposure associated with the handling of antineoplastic drugs (ADs) in the healthcare environment has been highlighted and demonstrated. Contamination was detected in both operating rooms (OR) and compounding units (CU), where healthcare workers handle and are exposed to ADs in different ways. In the OR, the risk of exposure is higher and the staff receives less training in handling ADs than in the CU. This study aimed to assess...

Supplemental Material for Epidemiological and clinical characteristics of vaccinated COVID-19 patients: A meta-analysis and systematic review by Wen Tian, Xingxiang Ren, Mei Han, Yuanyuan Zhang, Xu Gao, Zhihai Chen, and Wei Zhang in International Journal of Immunopathology and Pharmacology

Objective: With the global epidemic of coronavirus disease 2019 (COVID-19), vaccination rates are increasing globally. This study evaluated the relevant clinical manifestations of vaccinated COVID-19 patients. Methods: We searched carefully in 11 databases such as PubMed, Embase, Scopus, Cochrane Library, Web of Science, Ovid, China National Knowledge Infrastructure Database, Wan Fang Data, Sinomed, VIP Database, and Reading Showing Database up to 26 March 2022. To search for articles that have described the characteristics of vaccinated...

Histological changes in somalian patients with autoimmune hepatitis.

Histological changes in somalian patients with autoimmune hepatitis.

Additional file 3: Fig. S2. Differential methylation of CpG loci in the SOCS3 promoter in T-LGL samples. Comparison of CpG methylation (beta-value) for CpGs in the SOCS3 promoter between CD8+ memory cells (CD8mem) and T-LGLL samples (LGL). On top, adjusted p val of differential methylation analysis (dmpFDR, top left) and adjusted p value of differential variability analysis (dmVar, top right).

Additional file 5: Fig. S3B. Gene Ontology analysis of genes hyper- (A) and hypomethylated (B) of T-LGL patients. Significant Biological processes (GO database) enriched in genes associated with significantly differentially methylated CpG loci in T-LGL. Enrichment represented as odds ratio. Point size represents the gene count of each pathway. Enrichment p value obtained by overrepresentation analysis [30], represented by point color. B Gene Ontology analysis of hypomethylated genes in T-LGL.

Abstract Background The molecular pathogenesis of T-cell large granular lymphocytic leukemia (T-LGLL), a mature T-cell leukemia arising commonly from T-cell receptor αβ-positive CD8+ memory cytotoxic T cells, is only partly understood. The role of deregulated methylation in T-LGLL is not well known. We analyzed the epigenetic profile of T-LGLL cells of 11 patients compared to their normal counterparts by array-based DNA methylation profiling. For identification of molecular events driving the pathogenesis of T-LGLL, we compared...

Objective: With the global epidemic of coronavirus disease 2019 (COVID-19), vaccination rates are increasing globally. This study evaluated the relevant clinical manifestations of vaccinated COVID-19 patients. Methods: We searched carefully in 11 databases such as PubMed, Embase, Scopus, Cochrane Library, Web of Science, Ovid, China National Knowledge Infrastructure Database, Wan Fang Data, Sinomed, VIP Database, and Reading Showing Database up to 26 March 2022. To search for articles that have described the characteristics of vaccinated...