47 Works

Fungal fruit body assemblages are tougher in harsh microclimates

Franz-Sebastian Krah, Jonas Hagge, Jasper Schreiber, Roland Brandl, Jörg Müller & Claus Bässler
Forest species are affected by macroclimate, however, the microclimatic variability can be more extreme and change through climate change. Fungal fruiting community composition was affected by microclimatic differences. Here we ask whether differences in the fruiting community can be explained by morphological traits of the fruit body, which may help endure harsh conditions. We used a dead wood experiment and macrofungal fruit body size, color, and toughness. We exposed logs of two host tree species...

Anatomic and optical parameters of three species of penguin (Aves: Sphenisciformes: Spheniscidae)

Peter W. Hadden, Misha Vorobyev, Stephanie B. Cassidy, Akilesh Gokul, Samantha K. Simkin, Henry Tran, Charles N.J. McGhee & Jie Zhang
This collection contains raw data obtained from in vivo and post mortem examination of the eyes of little penguins (Eudyptula minor) from Auckland, New Zealand, as well as captive king (Aptenodytes patagonicus) and gentoo penguins (Pygoscelis papua) descended from animals living on South Georgia. Included are A & B ultrasound recordings, corneal topography, photographs taken during keratoscopy, anterior segment optical coherence tomography scans and recordings made of the pupil of king, little and gentoo penguins.

Phylogenetic relatedness of food plants reveals highest insect herbivore specialization at intermediate temperatures along a broad climatic gradient

Sebastian König, Jochen Krauss, Alexander Keller & Ingolf Steffan-Dewenter
Phytophagous insects differ in their degree of specialisation, biased by resource availability. The composition and richness of herbivore and plant assemblages change along climatic gradients, but knowledge about associated shifts in specialisation is scarce and lacks controlling for abundance and phylogeny of interaction partners. Thus, we aimed to test whether the specialisation of herbivores in insect- plant – interaction networks decreases towards cold habitats as predicted by the ‘altitude niche-breadth hypothesis’ to forecast possible consequences...

Climate and land-use effects on dung beetle assemblages

Jana Englmeier, Christian Von Hoermann, Daniel Rieker, Eric M. Benbow, Caryl Benjamin, Ute Fricke, Cristina Ganuza, Maria Haensel, Tomáš Lackner, Oliver Mitesser, Sarah Redlich, Rebekka Riebl, Sandra Rojas-Botero, Thomas Rummler, Jörg-Alfred Salamon, David Sommer, Ingolf Steffan-Dewenter, Cynthia Tobisch, Johannes Uhler, Lars Uphus, Jie Zhang & Jörg Müller
This dataset contains data from a field study conducted in 2019 and described in the paper "Dung beetle diversity is mainly affected by land use, while community specialization is driven by climate" by Englmeier et al. To test the effects of land use and climate on α-diversity, local community specialization (H2'), and γ-diversity of dung beetles, we used pitfall traps baited with four different dung types at 114 study sites, distributed over a spatial extent...

The influence of baseline characteristics, treatment and depression on health-related quality of life in patients with multiple myeloma: a prospective observational study

Julia Fischer, Stefan Knop, Sophia Danhof, Hermann Einsele, Daniela Keller & Claudia Löffler
Abstract Background Multiple myeloma (MM) is the third most common hematologic malignancy with increasing importance due to improving treatment strategies and long-term outcomes in an aging population. This study aims to analyse influencing factors on health-related quality of life (HRQoL), such as treatment strategies, participation in a clinical trial and patient characteristics like anxiety, depression, gender, and age. A better understanding of the individual factors in context with HRQoL could provide a helpful instrument for...

The influence of baseline characteristics, treatment and depression on health-related quality of life in patients with multiple myeloma: a prospective observational study

Julia Fischer, Stefan Knop, Sophia Danhof, Hermann Einsele, Daniela Keller & Claudia Löffler
Abstract Background Multiple myeloma (MM) is the third most common hematologic malignancy with increasing importance due to improving treatment strategies and long-term outcomes in an aging population. This study aims to analyse influencing factors on health-related quality of life (HRQoL), such as treatment strategies, participation in a clinical trial and patient characteristics like anxiety, depression, gender, and age. A better understanding of the individual factors in context with HRQoL could provide a helpful instrument for...

USP38 inhibits colorectal cancer cell proliferation and migration via downregulating HMX3 ubiquitylation

Jun Wang, Yongxing Gu, Xueqin Yan, Jie Zhang & Yong Ding
Accumulating evidence has shown that H6 Family Homeobox 3 (HMX3) plays a crucial role in nervous system regulation. However, the regulatory mechanism of HMX3 in colorectal cancer (CRC) has seldom been studied. Herein, HMX3 was significantly downregulated in CRC, as demonstrated by qRT-PCR and WB analysis on clinical samples and a panel of cell lines. Besides, it was found that the expression of HMX3 was negatively correlated with survival of CRC patients. The functional analyses...

High variation in hydraulic efficiency but not xylem safety between roots and branches in four temperate broad-leaved tree species

Torben Lübbe, Laurent J. Lamarque, Sylvain Delzon, José M. Torres-Ruiz, Régis Burlett, Christoph Leuschner & Bernhard Schuldt
Xylem hydraulic safety and efficiency are key traits determining tree fitness in a warmer and drier world. While numerous plant hydraulic studies have focused on branches, our understanding of root hydraulic functioning remains limited, although roots control water uptake, influence stomatal regulation and have commonly been considered as the most vulnerable organ along the hydraulic pathway. We investigated 11 traits related to xylem safety and efficiency along the hydraulic pathway in four temperate broad-leaved tree...

Metabarcoding of canopy arthropods reveals negative impacts of forestry insecticides on community structure across multiple taxa

Benjamin Leroy, Sebastian Seibold, Jérôme Morinière, Vedran Bozicevic, Jessica Jaworek, Nicolas Roth, Sebastian Vogel, Sharon Zytynska, Ralf Petercord, Peter Eichel & Wolfgang Weisser
1. Insecticides used to combat outbreaks of forest defoliators can adversely affect non-target arthropods. Forest use insecticides typically suppress Lepidoptera larvae which are the keystone of the canopy community of deciduous oak forests. The abrupt removal of this dominant component of the food web could have far-reaching implications for forest ecosystems, yet it is rarely investigated in practice owing to several methodological shortcomings. The taxonomic impediment and the biased nature of arthropod sampling techniques particularly...

High-resolution 3D forest structure explains ecomorphological trait variation in assemblages of saproxylic beetles

Lukas Drag, Ryan Burner, Jörg Stephan, Tone Birkemoe, Inken Doerfler, Martin M. Gossner, Paul Magdon, Otso Ovaskainen, Mária Potterf, Peter Schall, Tord Snäll, Anne Sverdrup-Thygeson, Wolfgang Weisser & Jörg Müller
1. Climate, topography, and the 3D structure of forests are major drivers affecting local species communities. However, little is known about how the specific functional traits of saproxylic (wood-living) beetles, involved in the recycling of wood, might be affected by those environmental characteristics. 2. Here we combine ecological and morphological traits available for saproxylic beetles and airborne laser scanning (ALS) data in Bayesian trait-based joint species distribution models to study how traits drive the distributions...

Long-Term Outcome of Corneal Collagen Crosslinking with Riboflavin and UV-A Irradiation for Keratoconus*

Franziska K. Seifert, Johanna Theuersbacher, Dorothee Schwabe, Olga Lamm, Jost Hillenkamp & Daniel Kampik
To evaluate long-term outcomes of corneal collagen crosslinking (CXL) using riboflavin and UV-A irradiation and to determine when to repeat CXL. In this retrospective consecutive interventional case series 131 eyes of 131 patients (95 male, 36 female, mean age 29.7 ± 11.4 years) between 2006 and 2016 received standard CXL (Dresden protocol, epithelium-off) for progressive keratoconus. Corrected distance visual acuity (CDVA) and corneal tomography (K1, K2, Kmax) were repeatedly recorded 1 year (n = 103...

Additional file 1 of Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase

Nastasja Merle, Sabrina Elmshäuser, Florian Strassheimer, Michael Wanzel, Alexander M. König, Julianne Funk, Michelle Neumann, Katharina Kochhan, Frederik Helmprobst, Axel Pagenstecher, Andrea Nist, Marco Mernberger, André Schneider, Thomas Braun, Tilman Borggrefe, Rajkumar Savai, Oleg Timofeev & Thorsten Stiewe
Additional file 1: Survival of non-GLuc transgenic SCLC mice. LSL-Cas9 mice (without GLuc transgene) were intratracheally infected with either AV-C.Cre, AV-PR.Cre or AV-PRL.Cre as depicted in Fig. 6a. Shown is the Kaplan-Meier survival plot with group size, median survival and P-values from Log-rank (Mantel-Cox) test

Additional file 4 of Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase

Nastasja Merle, Sabrina Elmshäuser, Florian Strassheimer, Michael Wanzel, Alexander M. König, Julianne Funk, Michelle Neumann, Katharina Kochhan, Frederik Helmprobst, Axel Pagenstecher, Andrea Nist, Marco Mernberger, André Schneider, Thomas Braun, Tilman Borggrefe, Rajkumar Savai, Oleg Timofeev & Thorsten Stiewe
Additional file 4: Primers used for sgRNA off-target analysis.

Data from: The rising moon promotes mate finding in moths

Jacqueline Degen
To counteract insect decline, it is essential to understand the underlying causes, especially for key pollinators such as nocturnal moths whose ability to orientate can easily be influenced by ambient light conditions. These comprise natural light sources as well as artificial light, but their specific relevance for moth orientation is still unknown. We investigated the influence of moonlight on the reproductive behavior of privet hawkmoths (Sphinx ligustri) at a relatively dark site where the Milky...

Data from: Nectar robbing rather than pollinator availability constrains reproduction of a bee-flowered plant at high elevations

Patrick Laurenz Kohl & Ingolf Steffan-Dewenter
The files contain data on the floral ecology of Clinopodium alpinum collected along an elevational gradient in the Berchtesgaden National Park in Germany in 2017. The data are presented in the work entitled "Nectar robbing rather than pollinator availability constrains reproduction of a bee-flowered plant at high elevations" by Patrick L. Kohl and Ingolf Steffan-Dewenter. Files include data on flower visitor observations (including raw data on the number of visits by individual specifmens), data on...

Anatomic and optical parameters of three species of penguin (Aves: Sphenisciformes: Spheniscidae)

Peter W. Hadden, Misha Vorobyev, Stephanie B. Cassidy, Akilesh Gokul, Samantha K. Simkin, Henry Tran, Charles N.J. McGhee & Jie Zhang
This collection contains raw data obtained from in vivo and post mortem examination of the eyes of little penguins (Eudyptula minor) from Auckland, New Zealand, as well as captive king (Aptenodytes patagonicus) and gentoo penguins (Pygoscelis papua) descended from animals living on South Georgia. Included are A & B ultrasound recordings, corneal topography, photographs taken during keratoscopy, anterior segment optical coherence tomography scans and recordings made of the pupil of king, little and gentoo penguins.

Alpine butterflies want to fly high: Species and communities shift upwards faster than their host plants

Janika M. Kerner, Jochen Krauss, Fabienne Maihoff, Lukas Bofinger & Alice Classen
Despite sometimes strong co-dependencies of insect herbivores and plants, responses of individual taxa to accelerating climate change are typically studied in isolation. Thereby, biotic interactions that potentially limit species in tracking their preferred climatic niches are ignored. Here, we chose butterflies as a prominent representative of herbivorous insects to investigate the impacts of temperature changes and their larval host plant distributions along a 1.4 km elevational gradient in the German Alps. Following a sampling protocol...

Additional file 2 of Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase

Nastasja Merle, Sabrina Elmshäuser, Florian Strassheimer, Michael Wanzel, Alexander M. König, Julianne Funk, Michelle Neumann, Katharina Kochhan, Frederik Helmprobst, Axel Pagenstecher, Andrea Nist, Marco Mernberger, André Schneider, Thomas Braun, Tilman Borggrefe, Rajkumar Savai, Oleg Timofeev & Thorsten Stiewe
Additional file 2: Mutation spectra of SCLC cell lines. Cell lines were established from AV-PR.Cre (n=3) and AV-PRL.Cre (n=5) SCLC tumors and analyzed by deep sequencing of the sgRNA target regions in Trp53, Rb1 and Rbl2. Depicted is the frequency of wild-type and mutant reads. For mutant reads, the Top5 mutations are color-coded as deletions or insertions and labelled with the number of deleted, inserted or substituted base pairs. Less frequent mutations are summarized as...

Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase

Nastasja Merle, Sabrina Elmshäuser, Florian Strassheimer, Michael Wanzel, Alexander M. König, Julianne Funk, Michelle Neumann, Katharina Kochhan, Frederik Helmprobst, Axel Pagenstecher, Andrea Nist, Marco Mernberger, André Schneider, Thomas Braun, Tilman Borggrefe, Rajkumar Savai, Oleg Timofeev & Thorsten Stiewe
Abstract Background In vivo gene editing of somatic cells with CRISPR nucleases has facilitated the generation of autochthonous mouse tumors, which are initiated by genetic alterations relevant to the human disease and progress along a natural timeline as in patients. However, the long and variable, orthotopic tumor growth in inner organs requires sophisticated, time-consuming and resource-intensive imaging for longitudinal disease monitoring and impedes the use of autochthonous tumor models for preclinical studies. Methods To facilitate...

Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase

Nastasja Merle, Sabrina Elmshäuser, Florian Strassheimer, Michael Wanzel, Alexander M. König, Julianne Funk, Michelle Neumann, Katharina Kochhan, Frederik Helmprobst, Axel Pagenstecher, Andrea Nist, Marco Mernberger, André Schneider, Thomas Braun, Tilman Borggrefe, Rajkumar Savai, Oleg Timofeev & Thorsten Stiewe
Abstract Background In vivo gene editing of somatic cells with CRISPR nucleases has facilitated the generation of autochthonous mouse tumors, which are initiated by genetic alterations relevant to the human disease and progress along a natural timeline as in patients. However, the long and variable, orthotopic tumor growth in inner organs requires sophisticated, time-consuming and resource-intensive imaging for longitudinal disease monitoring and impedes the use of autochthonous tumor models for preclinical studies. Methods To facilitate...

Low toxicity crop fungicide (Fenbuconazole) impacts reproductive male quality signals leading to a reduction of mating success in a wild solitary bee

Samuel Boff, Taina Conrad, Josué Raizer, Marten Wehrhahn, Melis Bayer, Anna Friedel, Panagiotis Theodorou, Thomas Schmitt & Daniela Lupi
Recent reports on bee health suggest that sub-lethal doses of pesticides have negative effects on wild bee reproduction and ultimately on their population growth. Females of the solitary horned mason bee, Osmia cornuta, evaluate thoracic vibrations and odours of males to assess male quality. When certain criteria are met, the female accepts the male and copulates. However, these signals were found to be modified by sub-lethal doses of pesticides in other hymenopterans. Here, we tested...

Blast output from: Lost in dead wood? Environmental DNA sequencing from dead wood shows little signs of saproxylic beetles

Nathalie Winiger, Sebastian Seibold, Kay Lucek, Jörg Müller & Gernot Segelbacher
eDNA metabarcoding has become a standard method for assessing wood-inhabiting fungi and bacteria, yet determination of dead-wood-inhabiting beetles still relies on time-consuming collection of beetle specimens. We thus tested whether beetle species can be identified by eDNA sequencing of wood in a mesocosm experiment that manipulated species assemblages. Dead wood samples were taken at exit holes of beetles and DNA was extracted and analyzed using two comparative methods: (i) metabarcoding with standard arthropod primers (421...

The role of diversity, body size and climate in dung removal: a correlative and experimental approach

Friederike Gebert, Ingolf Steffan-Dewenter, Patrick Kronbach & Marcell Peters
The mechanisms by which climatic changes influence ecosystem functions, i.e. by a direct climatic control of ecosystem processes or by modifying richness and trait compositions of species communities, remain unresolved. This study is a contribution to this discourse by elucidating the linkages between climate, land use, biodiversity, body size and ecosystem functions. We disentangled direct climatic from biodiversity-mediated effects by using dung removal by dung beetles as a model system and by combining correlative field...

Light and malaise traps tell different stories about the spatial variations in arthropod biomass and method-specific insect abundance

Annika Busse, Claus Bässler, Roland Brandl, Nicolas Friess, Hermann Hacker, Lea Heidrich, Torben Hilmers, Gisela Merkel-Wallner, Christian Schmid-Egger, Linda Seifert & Jörg Müller
1. Conclusions reached in meta-analyses of changes in insect communities may be influenced by method-specific sampling biases, which may lead to inappropriate conservation measures. 2. We argue that the contradictory conclusions regarding terrestrial insect biomass, abundance and richness patterns are, at least partly, due to methodological limitations that reflect taxon-specific responses to environmental changes. 3. In this study, light and Malaise traps were simultaneously deployed to sample insects at 52 plots in a temperate forest...

Anatomic and optical parameters of three species of penguin (Aves: Sphenisciformes: Spheniscidae)

Peter W. Hadden, Misha Vorobyev, Stephanie B. Cassidy, Akilesh Gokul, Samantha K. Simkin, Henry Tran, Charles N.J. McGhee & Jie Zhang
This collection contains raw data obtained from in vivo and post mortem examination of the eyes of little penguins (Eudyptula minor) from Auckland, New Zealand, as well as captive king (Aptenodytes patagonicus) and gentoo penguins (Pygoscelis papua) descended from animals living on South Georgia. Included are A & B ultrasound recordings, corneal topography, photographs taken during keratoscopy, anterior segment optical coherence tomography scans and recordings made of the pupil of king, little and gentoo penguins.

Registration Year

  • 2022
    47

Resource Types

  • Dataset
    24
  • Text
    12
  • Collection
    9
  • Book Chapter
    1
  • Output Management Plan
    1

Affiliations

  • University of Würzburg
    47
  • University of Bern
    14
  • Max Planck Institute for Heart and Lung Research
    9
  • Philipps University of Marburg
    9
  • University of Giessen
    9
  • Lanzhou Veterinary Research Institute
    5
  • Boston Children's Hospital
    5
  • Jilin University
    5
  • Xiamen University
    5
  • Agricultural Information Institute
    5