The establishment of a simple and effective method for determination of heavy metal ions has great significance due to the high hazard of heavy metal ions to public health and environments. Herein, we developed a facile way to synthesise a nanocomposite comprised of carbon nanotubes (CNTs) and metal-organic frameworks (MOFs), and explored its application in constructing an electrochemical (EC) sensor for detection of Cd2+ in aqueous solutions. The sensing surface was simply fabricated by casting...

Abstract Background Anti-thymoglobulin (ATG)-based immunosuppressive treatment (IST) is the standard first-line management for patients with severe AA/very severe AA (SAA/VSAA) and is not suitable for allogeneic stem cell transplantation. The response predictor was not fully investigated. Objective The present study attempted to explore other characteristics, such as serum lipid changes, during ATG-based IST and analyzed their significance in predicting IST response and survival. Methods A total of 61 newly diagnosed SAA/VSAA patients who received ATG-based...

Two new tigliane- and daphnane-type diterpenoids, given the trivial names daphnegens A–B (1–2) were isolated from the buds of Daphne genkwa. Their structures were assigned on the basis of extensive spectroscopic. The absolute configurations of both compounds were determined by comparison of their calculated and experimental CD curves. In addition, compounds 1–2 were tested for their cytotoxic activities against MCF-7 and HepG-2 human cancer cell lines, and compound 2 showed remarkable cytotoxic activity against HepG-2...

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Supplemental material, sj-docx-1-pie-10.1177_09544089221097360 for Sealing mechanism of large size and large deformation rubber cylinder for pipeline intelligent plugging robot under multiple factors by Yang Tang, Yuan Wang, Peng Sun and Xiang Liu in Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering

Additional file 1: Table S1. The host range of the global top two most occurred Colletotrichum species. Data were largely retrieved on July 8, 2022 via website search at https://nt.ars-grin.gov/fungaldatabases/ and analyzed at https://www.iplant.cn/ . Y for hosted. Gray for common hosts shared by C. siamense and C. fructicola, orange for hosts of C. siamense only, light blue for those of C. fructicola. Letters in red for records from previous publication (Talhinhas and Baroncelli 2021).

Abstract Anthracnose caused by Colletotrichum species, is one of the most destructive diseases affecting strawberry production worldwide. Fungi of the genus Colletotrichum, being ancient and co-evolved with flowering plants, rank eighth among the top ten economically important fungal pathogens and have diversified to adapt to various hosts. Since it was first reported in United States in 1931, strawberry anthracnose has spread globally, resulting in devastating economic, social, and ecological consequences. In a sense, to produce...

Cancer immunotherapy is a strategy that is moving to the frontier of cancer treatment in the current decade. In this study, we show evidence that 3-(2-nitrophenyl) propionic acid-paclitaxel nanoparticles (NPPA-PTX NPs), act as immunogenic cell death (ICD) inducers, stimulating an antitumor response which results in synergistic antitumor activity by combining anti-PD-L1 antibody (aPD-L1) in vivo. To investigate the antitumor immunity induced by NPPA-PTX NPs, the expression of both ICD marker calreticulin (CRT) and high mobility...

Here six phosphorescent heteroleptic cyclometalated Ir (III) complexes were theoretically designed to identify some suitable OLED materials. (fppzb)2Ir(acac), (fppzb)2Ir(tpip), (dfbdp)2Ir(fppzb), (F-fppzb)2Ir(acac), (F-fppzb)2Ir(tpip), and (dfbdp)2Ir(F-fppzb) were studied via density functional theory (DFT) and time-dependent density functional theory (TD-DFT) methods. Here, acac denotes acetylacetonate, tpip denotes tetraphenylimido-diphosphinate, fppzb denotes 4-tert-butyl-2-(5-(trifluoromethyl)-4H-pyrazol-3-yl) pyridine and F-fppzb denotes 4-tert-butyl-2-(5-fluoro-4H-pyrazol-3-yl) pyridine. Further, we discuss the value of these materials from the electronic structure, spectral properties, ionisation potentials, electron affinities, and application prospects...

Here six phosphorescent heteroleptic cyclometalated Ir (III) complexes were theoretically designed to identify some suitable OLED materials. (fppzb)2Ir(acac), (fppzb)2Ir(tpip), (dfbdp)2Ir(fppzb), (F-fppzb)2Ir(acac), (F-fppzb)2Ir(tpip), and (dfbdp)2Ir(F-fppzb) were studied via density functional theory (DFT) and time-dependent density functional theory (TD-DFT) methods. Here, acac denotes acetylacetonate, tpip denotes tetraphenylimido-diphosphinate, fppzb denotes 4-tert-butyl-2-(5-(trifluoromethyl)-4H-pyrazol-3-yl) pyridine and F-fppzb denotes 4-tert-butyl-2-(5-fluoro-4H-pyrazol-3-yl) pyridine. Further, we discuss the value of these materials from the electronic structure, spectral properties, ionisation potentials, electron affinities, and application prospects...

In mammalian cells, cohesin acetyltransferases Esco1 and Esco2 acetylate cohesin subunit Smc3 to establish chromosome cohesion, ensuring the accurate chromosome segregation. However, we have previously documented that both Esco1 and Esco2 have unique substrates and roles in mouse oocyte meiosis I to orchestrate the meiotic progression, but whether these functions are conserved among species is still not determined. Here, we used porcine oocytes as a model to illustrate that Esco1 and Esco2 exerted conserved functions...

The establishment of a simple and effective method for determination of heavy metal ions has great significance due to the high hazard of heavy metal ions to public health and environments. Herein, we developed a facile way to synthesise a nanocomposite comprised of carbon nanotubes (CNTs) and metal-organic frameworks (MOFs), and explored its application in constructing an electrochemical (EC) sensor for detection of Cd2+ in aqueous solutions. The sensing surface was simply fabricated by casting...

MicroRNA (miR)-150-5p has been investigated in many studies, while the role of exosomal miR-150-5p from bone arrow mesenchymal stromal cells (BMSCs) on cerebral ischemia/reperfusion (I/R) injury is not fully explored. This research aims to probe the effects of exosomal miR-150-5p from BMSCs on cerebral I/R injury via regulating B-cell translocation gene 2 (TLR5). Bone marrow mesenchymal stem cell-derived exosomes (BMSCs-Exo) were isolated and identified. The middle cerebral artery occlusion (MCAO) rat model was established and...

MicroRNA (miR)-150-5p has been investigated in many studies, while the role of exosomal miR-150-5p from bone marrow mesenchymal stromal cells (BMSCs) on cerebral ischemia/reperfusion (I/R) injury is not fully explored. This research aims to probe the effects of exosomal miR-150-5p from BMSCs on cerebral I/R injury via regulating B-cell translocation gene 2 (TLR5). BMSCs were cultured and transfected with miR-150-5p mimic, then exosomes from BMSCs were extracted. The middle cerebral artery occlusion (MCAO) rat model...

Functional experiments indicated that curcumin displayed stronger inhibitory activity on the proliferation of cervical cancer HeLa cells, while resveratrol had a better inhibition effect on migration. Then, we compared the candidate target genes of curcumin and resveratrol in the treatment of cervical cancer through network pharmacology. GO enrichment results showed that curcumin exerted its anti-cervical cancer effect by regulating cell cycle mitosis, whereas resveratrol affected adhesion. Furthermore, the target genes were verified by molecular docking,...

Functional experiments indicated that curcumin displayed stronger inhibitory activity on the proliferation of cervical cancer HeLa cells, while resveratrol had a better inhibition effect on migration. Then, we compared the candidate target genes of curcumin and resveratrol in the treatment of cervical cancer through network pharmacology. GO enrichment results showed that curcumin exerted its anti-cervical cancer effect by regulating cell cycle mitosis, whereas resveratrol affected adhesion. Furthermore, the target genes were verified by molecular docking,...

Abstract Background Accumulating evidence implicates that gut fungi are associated with the pathogenesis of colorectal cancer (CRC). Our previous study has revealed that Candida tropicalis (C. tropicalis) promotes colorectal tumorigenesis by enhancing immunosuppressive function of myeloid-derived suppressor cells (MDSCs) and increasing accumulation of MDSCs, but the underlying mechanisms remain unestablished. Methods Bone marrow–derived MDSCs were stimulated with C. tropicalis. RNA-sequencing analysis was performed to screen the differentially expressed genes. Quantitative real-time PCR and western blot...

Supplemental material, sj-docx-1-acr-10.1177_02841851211043839 for Clinical evaluation of AI software for rib fracture detection and its impact on junior radiologist performance by Xiang Liu, Dijia Wu, Huihui Xie, Yufeng Xu, Lin Liu, Xiaofeng Tao and Xiaoying Wang in Acta Radiologica

Abstract Purpose This study aimed to analyse the genomic alteration profiles and immune characteristics of a cohort of Chinese cervical cancer patients to understand why certain patients benefited from molecular targeted therapies and immunotherapy as well as their prognostic significance. Methods PD-L1 expression and clinicopathological information were obtained from 98 cervical cancer patients. Differences in PD-L1 expression and gene mutations between squamous cell carcinoma (SCC) and adenocarcinoma (AC) were analysed by the chi-square test or...

Polycystic ovary syndrome (PCOS) is a common endocrine disorder that poses a great threat to women's health. MiR-1224-5p is downregulated in the follicular fluid of patients with PCOS, but its role remains largely unknown. In this study, mice were treated with dehydroepiandrosterone (DHEA) to establish an in vivo model of PCOS. We found that enhanced activation of NLRP3 inflammasome was accompanied by downregulation of miR-1224-5p in ovarian tissue of PCOS mice. The effect of miR-1224-5p...

Polycystic ovary syndrome (PCOS) is a common endocrine disorder that poses a great threat to women's health. MiR-1224-5p is downregulated in the follicular fluid of patients with PCOS, but its role remains largely unknown. In this study, mice were treated with dehydroepiandrosterone (DHEA) to establish an in vivo model of PCOS. We found that enhanced activation of NLRP3 inflammasome was accompanied by downregulation of miR-1224-5p in ovarian tissue of PCOS mice. The effect of miR-1224-5p...

Dysfunction of the glymphatic system, an intracranial clearance pathway that drains misfolded proteins, has been implicated in the onset of Parkinson’s disease (PD). Recently, the coupling strength of global blood-oxygen-level-dependent (gBOLD) signals and cerebrospinal fluid (CSF) inflow dynamics have been suggested to be an indicator of glymphatic function. Using resting-state functional magnetic resonance imaging (MRI), we quantified gBOLD–CSF coupling strength as the cross-correlation between baseline gBOLD and CSF inflow signals to evaluate glymphatic function and...

Severe COVID-19 appears to be disproportionately more common in children and adolescents since the emergence of Omicron. More evidence regarding vaccine effectiveness (VE) is urgently needed to assist policymakers in making decisions and minimize vaccine hesitancy among the public. This was a case-control study in the pediatric population using data extracted from the electronic health records database in Hong Kong. Individuals aged 3–17 with COVID-19 confirmed by polymerase chain reaction were included in the study....

BackgroundThe detection of rib fractures (RFs) on computed tomography (CT) images is time-consuming and susceptible to missed diagnosis. An automated artificial intelligence (AI) detection system may be helpful to improve the diagnostic efficiency for junior radiologists.PurposeTo compare the diagnostic performance of junior radiologists with and without AI software for RF detection on chest CT images.Materials and methodsSix junior radiologists from three institutions interpreted 393 CT images of patients with acute chest trauma, with and without...

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