959 Works

The effect of amiloride in decreasing albuminuria in patients with diabetic kidney diseases: a prospective, crossover, open-label study

Ruizhao Li, Zhiyong Xie, Li Zhang, Ying Huang, Jianchao Ma, Wei Dong, Zhilian Li, Yuanhan Chen, Huaban Liang, Yanhua Wu, Xingchen Zhao, Wenjian Wang, Zhiming Ye, Shuangxin Liu, Wei Shi & Xinling Liang
Diabetic kidney diseases (DKD) were the leading cause of End-stage renal diseases worldwide. Albuminuria was a target for treatment in DKD and decreasing albuminuria was particularly important for improving its prognosis. However, there is still a lack of specific treatment for DKD. We conducted a prospective, crossover, open-label study to investigate the effect of amiloride in patients with DKD. Safety and efficacy were assessed by monitoring urine protein creatinine ratio(uPCR), urinary albumin creatinine ratio (uACR),...

DFT investigates the mechanisms of cross-dehydrogenative coupling between heterocycles and acetonitrile

Cheng-Yu He, Hong-Xia Hou, Ming-Qiong Tong, Da-Gang Zhou & Rong Li
The mechanisms between CH3CN and 1,3-dimethyl-1H-indole, initiated by DCP (dicumyl peroxide), have been studied with the B3LYP-D3 method with def2TZVP basis set for all atoms, and the SMD model was applied to simulate the solvent of acetonitrile. The computational results show that the homolytic process of DCP, catalysed by CuCl or CuI, can decrease the energy barrier, and the recycle procedure of Cu catalyst has been described. Then the Csp3-H activation of CH3CN has been...

Exploring AI-2-mediated interspecies communications within rumen microbial communities

Xiaozhen Liu, Qinmeng Liu, Sihuai Sun, Hengxi Sun, Yao Wang, Xihui Shen & Lei Zhang
Abstract Background The rumen is an ecosystem with a complex microbial microflora in which microbes initiate biofilm formation by attaching to plant surfaces for plant degradation and are capable of converting feed to nutrients and energy via microbial processes. Quorum sensing (QS) is a cell-to-cell communication mechanism that allows microbes to synchronize the expression of multiple genes in the group to perform social behaviors such as chemotaxis and biofilm formation using self-synthesized QS signaling molecules....

Additional file 10 of Exploring AI-2-mediated interspecies communications within rumen microbial communities

Xiaozhen Liu, Qinmeng Liu, Sihuai Sun, Hengxi Sun, Yao Wang, Xihui Shen & Lei Zhang
Additional file 10: Table S6. Expression of the identified AI-2 synthases and receptors in rumen metatranscriptome datasets.

Additional file 12 of Exploring AI-2-mediated interspecies communications within rumen microbial communities

Xiaozhen Liu, Qinmeng Liu, Sihuai Sun, Hengxi Sun, Yao Wang, Xihui Shen & Lei Zhang
Additional file 12: Figure S6. Widespread occurrence of rumen bacteria in the communication network. (A) The occurrence of rumen bacteria in the network. (B) The occurrence of bacteria that synthesize AI-2 at the phylum level. (C) The occurrence of bacteria that synthesize and sense AI-2 at the phylum level. (D) The occurrence of bacteria that sense AI-2 at the phylum level. Numbers indicate the numbers of bacterial genomes in which the corresponding functions were found...

Additional file 14 of Exploring AI-2-mediated interspecies communications within rumen microbial communities

Xiaozhen Liu, Qinmeng Liu, Sihuai Sun, Hengxi Sun, Yao Wang, Xihui Shen & Lei Zhang
Additional file 14: Table S7. The network among the rumen bacterial taxa and AI-2.

Gapless genome assembly of East Asian finless porpoise, Neophocaena asiaeorientalis sunameri

Denghua Yin, Chunhai Chen, Danqing Lin, Jialun Zhang, Congping Ying, Yan Liu, Wang Liu, Zhichen Cao, Chenxi Zhao, Chenhe Wang, Liping Liang, Pao Xu, Jianbo Jian & Kai Liu
There are the genome sequence, gene set, coding sequence and protein sequence of Neophocaena asiaeorientalis sunameri.

Subspace Estimation with Automatic Dimension and Variable Selection in Sufficient Dimension Reduction

Jing Zeng, Qing Mai & Xin Zhang
Sufficient dimension reduction (SDR) methods target finding lower-dimensional representations of a multivariate predictor to preserve all the information about the conditional distribution of the response given the predictor. The reduction is commonly achieved by projecting the predictor onto a low-dimensional subspace. The smallest such subspace is known as the Central Subspace (CS) and is the key parameter of interest for most SDR methods. In this article, we propose a unified and flexible framework for estimating...

Construction of a transposase accessible chromatin landscape reveals chromatin state of repeat elements and potential causal variant for complex traits in pigs

Tao Jiang, Ziqi Ling, Zhimin Zhou, Xiaoyun Chen, Liqing Chen, Sha Liu, Yingchun Sun, Jiawen Yang, Bin Yang, Jianzhen Huang & Lusheng Huang
Abstract Background A comprehensive landscape of chromatin states for multiple mammalian tissues is essential for elucidating the molecular mechanism underlying regulatory variants on complex traits. However, the genome-wide chromatin accessibility has been only reported in limited tissue types in pigs. Results Here we report a genome-wide landscape of chromatin accessibility of 20 tissues in two female pigs at ages of 6 months using ATAC-seq, and identified 557,273 merged peaks, which greatly expanded the pig regulatory...

Gut microbiota differs between two cold-climate lizards distributed in thermally different regions

Jun-Qiong Chen, Lu-Wen Zhang, Ru-Meng Zhao, Hai-Xia Wu, Long-Hui Lin, Peng Li, Hong Li, Yan-Fu Qu & Xiang Ji
Abstract Background The metabolic cold-climate adaption hypothesis predicts that animals from cold environments have relatively high metabolic rates compared with their warm-climate counterparts. However, studies testing this hypothesis are sparse. Here, we compared gut microbes between two cold-climate lizard species of the genus Phrynocephalus to see if gut microbiota could help lizards adapt to cold environments by promoting metabolism. We conducted a 2 species (P. erythrurus and P. przewalskii) × 2 temperatures (24 and 30...

Additional file 7 of Gut microbiota differs between two cold-climate lizards distributed in thermally different regions

Jun-Qiong Chen, Lu-Wen Zhang, Ru-Meng Zhao, Hai-Xia Wu, Long-Hui Lin, Peng Li, Hong Li, Yan-Fu Qu & Xiang Ji
Supplementary Material 7

Gut microbiota differs between two cold-climate lizards distributed in thermally different regions

Jun-Qiong Chen, Lu-Wen Zhang, Ru-Meng Zhao, Hai-Xia Wu, Long-Hui Lin, Peng Li, Hong Li, Yan-Fu Qu & Xiang Ji
Abstract Background The metabolic cold-climate adaption hypothesis predicts that animals from cold environments have relatively high metabolic rates compared with their warm-climate counterparts. However, studies testing this hypothesis are sparse. Here, we compared gut microbes between two cold-climate lizard species of the genus Phrynocephalus to see if gut microbiota could help lizards adapt to cold environments by promoting metabolism. We conducted a 2 species (P. erythrurus and P. przewalskii) × 2 temperatures (24 and 30...

Additional file 6 of Transcriptomic analysis of paternal behaviors in prairie voles

Florian Duclot, Yan Liu, Samantha K. Saland, Zuoxin Wang & Mohamed Kabbaj
Additional file 6. Relationships between parental behavior test behaviors. For each pair of behavior, a linear model was fit. Each individual point represents a distinct animal, and the shaded area depicts the 95% confidence interval.

Transcriptomic analysis of paternal behaviors in prairie voles

Florian Duclot, Yan Liu, Samantha K. Saland, Zuoxin Wang & Mohamed Kabbaj
Abstract Background The importance of fathers’ engagement in care and its critical role in the offspring’s cognitive and emotional development is now well established. Yet, little is known on the underlying neurobiology due to the lack of appropriate animal models. In the socially monogamous and bi-parental prairie vole (Microtus ochrogaster), while 60–80% of virgin males show spontaneous paternal behaviors (Paternal), others display pup-directed aggression (Attackers). Here we took advantage of this phenotypic dichotomy and used...

Transcriptomic analysis of paternal behaviors in prairie voles

Florian Duclot, Yan Liu, Samantha K. Saland, Zuoxin Wang & Mohamed Kabbaj
Abstract Background The importance of fathers’ engagement in care and its critical role in the offspring’s cognitive and emotional development is now well established. Yet, little is known on the underlying neurobiology due to the lack of appropriate animal models. In the socially monogamous and bi-parental prairie vole (Microtus ochrogaster), while 60–80% of virgin males show spontaneous paternal behaviors (Paternal), others display pup-directed aggression (Attackers). Here we took advantage of this phenotypic dichotomy and used...

Additional file 22 of Transcriptomic analysis of paternal behaviors in prairie voles

Florian Duclot, Yan Liu, Samantha K. Saland, Zuoxin Wang & Mohamed Kabbaj
Additional file 22. Structure-specific associations of gene coexpression modules with parental behaviors. The correlation of each co-expression module from the weighted gene coexpression network analysis with behavioral traits (behaviors scored during parental behavior test and phenotype status) is depicted for each structure. The correlation value is detailed alongside its corresponding p-value in parentheses. Note that within each structure, only modules with at least one significant association are depicted. MPOA: medial preoptic area, NAc: nucleus accumbens,...

Additional file 24 of Transcriptomic analysis of paternal behaviors in prairie voles

Florian Duclot, Yan Liu, Samantha K. Saland, Zuoxin Wang & Mohamed Kabbaj
Additional file 24. Functional enrichment of gene ontologies of the biological processes category in gene co-expression modules derived from the weighted gene coexpression network analysis (WGCNA).

Additional file 16 of Transcriptomic analysis of paternal behaviors in prairie voles

Florian Duclot, Yan Liu, Samantha K. Saland, Zuoxin Wang & Mohamed Kabbaj
Additional file 16. Representation of the number of genes differentially expressed in the medial preoptic area (MPOA, top), nucleus accumbens (NAc, middle), and lateral septum (LS, bottom) across all chromosomes and linkage groups in the prairie vole assembly. Only cases with at least one differentially expressed gene are depicted.

Additional file 17 of Transcriptomic analysis of paternal behaviors in prairie voles

Florian Duclot, Yan Liu, Samantha K. Saland, Zuoxin Wang & Mohamed Kabbaj
Additional file 17. Functional enrichment of genes differentially expressed in the nucleus accumbens (NAc). For each pairwise comparison, the functional enrichments in gene ontologies of the biological processes (A), cellular components (B), and molecular functions (C) categories as well as in pathways from the Kyoto Encyclopedia of Genes and Genomes (KEGG, D) were tested for the differentially expressed genes up- or down-regulated (UP, and DOWN, respectively). The labels of pairwise comparisons were coded in two...

Heart-targeting exosomes from human cardiosphere-derived cells improve the therapeutic effect on cardiac hypertrophy

Liang Mao, Yun-Da Li, Ruo-Lan Chen, Gang Li, Xiao-Xia Zhou, Fei Song, Chan Wu, Yu Hu, Yi-Xiang Hong, Xitong Dang, Gui-Rong Li & Yan Wang
Abstract Exosomes of human cardiosphere-derived cells (CDCs) are very promising for treating cardiovascular disorders. However, the current challenge is inconvenient delivery methods of exosomes for clinical application. The present study aims to explore the potential to enhance the therapeutic effect of exosome (EXO) from human CDCs to myocardial hypertrophy. A heart homing peptide (HHP) was displayed on the surface of exosomes derived from CDCs that were forced to express the HHP fused on the N-terminus...

Additional file 1 of Heart-targeting exosomes from human cardiosphere-derived cells improve the therapeutic effect on cardiac hypertrophy

Liang Mao, Yun-Da Li, Ruo-Lan Chen, Gang Li, Xiao-Xia Zhou, Fei Song, Chan Wu, Yu Hu, Yi-Xiang Hong, Xitong Dang, Gui-Rong Li & Yan Wang
Additional file 1: Figure S1. Schematic illustration of displaying a homing peptide/FLAG on the surface of exosomes. The coding sequence of HHP/FLAG (red bar) was fused in-frame to the LAMP2b cDNA between the signal peptide (SP) and the N-terminus, which was then cloned into pLVX-IRES-ZsGreen1 expression plasmid. Forced expression of the plasmid in CDCs would display the HHP/FLAG (red oval) on the surface of exosomes. Figure S2. Schematic illustration of mice treatment schedule. The TAC...

Additional file 1 of Interaction of miR-200a-3p with YAP regulates cell proliferation and metastasis differentially in HPV-positive and HPV-negative cervical cancer cells

Hong Chen, Lingling Gu, Min Zhang, Huifen Chen, Hong Liao, Xueping Cao, Lu Yu & Jun Zhang
Additional file 1: Supplementary Fig. S1. qRT-PCRassessmentsoftransfectionefficiencies The transfection efficiencies of miR-200a-3p mimics (A), sh-miR-200a-3p (B), and YAP(C)wereperformedinC33A,SihaandHelacells.*p<0.05, **p<0.01,***p<0.001,ns:notsignificant. The authors declare that they have no competing interests. Supplementary Fig. S2. themRNAandproteinlevelsofYAPinHelacells Significant decreases in levels of YAP mRNA (A) and YAP protein (B) following up-regulations of miR-200a-3p, or significant increases in levels of YAP mRNA (C) and protein (D) following down-regulations of miR-200a-3p were not observed in Helacells.*p<0.05,**p<0.01,***p<0.001,ns:notsignificant.

Interaction of miR-200a-3p with YAP regulates cell proliferation and metastasis differentially in HPV-positive and HPV-negative cervical cancer cells

Hong Chen, Lingling Gu, Min Zhang, Huifen Chen, Hong Liao, Xueping Cao, Lu Yu & Jun Zhang
Abstract Background Although evidence has revealed that miR-200a-3p is involved in the malignant progression of various tumors, the regulatory mechanism of miR-200a-3p in the development of cervical cancer (CC) cells with different HPV statuses remains unknown. The present study was to investigate the differential effects of either miR-200a-3p or YAP on tumorous cells’ fate in vitro in HPV-negative and HPV-positive cervical cancer cell models, and to explore if the changes in proliferation, migration, and invasion...

Additional file 3 of Interaction of miR-200a-3p with YAP regulates cell proliferation and metastasis differentially in HPV-positive and HPV-negative cervical cancer cells

Hong Chen, Lingling Gu, Min Zhang, Huifen Chen, Hong Liao, Xueping Cao, Lu Yu & Jun Zhang
Additional file 3.

Using a WeChat mini-program-based lactation consultant intervention to increase the consumption of mother’s own milk by preterm infants in the neonatal intensive care unit: a study protocol for a cluster randomized controlled trial

Jie Huo, Xinping Wu, Chuanli Gu, Zhangbin Yu, Jun Zhang, Xiaohui Chen, Jingai Zhu, Feng Liu, Beibei Liu, Qianqian Li & Shuping Han
Abstract Background The benefits of mother’s own milk (MOM) for preterm infants have been widely recognized. Many studies have shown that the rate of breastfeeding of premature infants remains very low. Although many studies use measures to promote breastfeeding, few high-quality cluster randomized controlled studies have evaluated the effectiveness of these measures. WeChat is an instant messaging software for smart terminals, and WeChat mini-programs have been widely used to promote health and self-management in China....

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