The mammalian gut harbors a very complex and diverse microbiota that play an important role in the intestinal homeostasis and host health. Exposure to radiation results in dysbiosis of the gut microbiota leading to detrimental pathophysiological changes to the host. To alleviate the effects of irradiation, several candidate countermeasures are under investigation. BIO 300 is a promising candidate under advanced development. The aim of this study was to investigate the effects of BIO 300 on...

Global changes in response to human encroachment into natural habitats and carbon emissions are driving the biodiversity extinction crisis and increasing disease emergence risk. Host distributions are one critical component to identify areas at risk of viral spillover, and bats act as reservoirs of diverse viruses. We developed a reproducible ecological niche modelling pipeline for bat hosts of SARS-like viruses (subgenus Sarbecovirus), given that several closely-related viruses have been discovered and sarbecovirus-host interactions have gained...

Electrocatalytic CO2 reduction to fuel is one of the important ways to solve energy and environmental problems. In this work, the preparation of hollow Aux-Cu2O electrocatalyst and the performance of electrocatalytic CO2 reduction to ethanol were studied. Hollow Cu2O nanospheres were prepared by a soft template method, and Aux-Cu2O composites were prepared by galvanic replacement. The characterization results of XRD and XPS reveal that Cu+ is the main chemical state of Cu in the catalysts....

The antimicrobial activity and preservative effect of Ceylon spinach (Basella alba L.) seed protein extract on sturgeon fillets stored at 4°C were investigated in this study. The results showed that Ceylon spinach seed protein extract (CSSPE) had inhibitory effect on 33 microorganisms, including 1 fungus and 32 bacteria (25 gram-negative bacteria and 7 gram-positive bacteria). In addition, CSSPE inhibited bacterial growth (total viable counts, Pseudomonas, Aeromonas and psychrotrophs), suppressed ATP degradation and retained good-quality characteristic...

Additional file 4: Supplemental Table 3. Protein Ontology representation of SARS-CoV-2 proteins. Comparative information in RefSeq and UniProtKB is also provided.

Abstract Background The current COVID-19 pandemic and the previous SARS/MERS outbreaks of 2003 and 2012 have resulted in a series of major global public health crises. We argue that in the interest of developing effective and safe vaccines and drugs and to better understand coronaviruses and associated disease mechenisms it is necessary to integrate the large and exponentially growing body of heterogeneous coronavirus data. Ontologies play an important role in standard-based knowledge and data representation,...

The massive application of agrochemicals and associated environmental residues greatly threaten human health. Cardiac dysfunction, as a major contributing factor to human mortality, is increasingly reported in health risk assessments to be associated with agrochemical exposure. In this mini-review, we summarize the cardiotoxicity of various agrochemicals including herbicides, insecticides, fungicides, and acaricides on zebrafish embryos/larvae and systematically discuss the role of cardiac development-related pathway disorders, imbalance in Ca2+ homeostasis, mitochondrial dysfunction, and general negative effects...

Detection of virus-like particles quantitively under a low concentration is of vital importance for early infection diagnosis and water pollution analysis. In this paper, a novel virus detection method is proposed using indirect polarization parametric imaging method combined with a plasmonic split-ring nanocavity array coated with an Au film and a quantitative algorithm is implemented based on the extended Laplace operator. The attachment of viruses to the split-ring cavity breaks the structural symmetry, and such...

Detection of virus-like particles quantitively under a low concentration is of vital importance for early infection diagnosis and water pollution analysis. In this paper, a novel virus detection method is proposed using indirect polarization parametric imaging method combined with a plasmonic split-ring nanocavity array coated with an Au film and a quantitative algorithm is implemented based on the extended Laplace operator. The attachment of viruses to the split-ring cavity breaks the structural symmetry, and such...

In this study, we presented a case series to highlight the chromosomal microarray (CMA) in identifying chromosomal abnormalities which is undetectable by conventional karyotyping or known abnormal chromosomes without clear diagnosis. Extensive studies showed that CMA was gradually accepted as a prenatal invasive testing during pregnancy. The aim of this study was to evaluate the diagnostic effect of CMA for foetuses with abnormal chromosomes unrecognised by conventional karyotyping. Pregnant women who need prenatal diagnosis with...

The goal of the current study was to identify longitudinal changes in urinary metabolites following IR exposure and to determine potential alleviation of radiation toxicities by administration of recombinant APC formulations. Female adult WAG/RijCmcr rats were irradiated with 13.0 Gy leg-out partial body X-rays; longitudinally collected urine samples were subject to LC-MS based metabolomic profiling. Sub-cohorts of rats were treated with three variants of recombinant APC namely, rat wildtype (WT) APC, rat 3K3A mutant form...

Introduction:The differential diagnosis of pleural effusion is difficult, and studies have reported on the potential role of adenosine deaminase (ADA) in the differential diagnosis of undiagnosed pleural effusion. This retrospective study aimed to investigate the diagnostic role of ADA in pleural effusion.Methods:266 patients with pleural effusion from three centers were enrolled. The concentrations of ADA and lactate dehydrogenase (LDH) were measured in pleural fluids and serum samples of the patients. The diagnostic performance of ADA-based...

Introduction:The differential diagnosis of pleural effusion is difficult, and studies have reported on the potential role of adenosine deaminase (ADA) in the differential diagnosis of undiagnosed pleural effusion. This retrospective study aimed to investigate the diagnostic role of ADA in pleural effusion.Methods:266 patients with pleural effusion from three centers were enrolled. The concentrations of ADA and lactate dehydrogenase (LDH) were measured in pleural fluids and serum samples of the patients. The diagnostic performance of ADA-based...

Supplemental material, sj-docx-1-tar-10.1177_17534666231155747 for Adenosine deaminase-based measurement in the differential diagnosis of pleural effusion: a multicenter retrospective study by Lijuan Gao, Wujun Wang, Ying Zhang, Xueru Hu, Jing An, Yang Li, Mei Chen and Yongchun Shen in Therapeutic Advances in Respiratory Disease

Breast cancer is one of the most common types of cancer in female patients with high morbidity and mortality. Multi-drug chemotherapy has significant advantages in the treatment of malignant tumors, especially in reducing drug toxicity, increasing drug sensitivity and reducing drug resistance. The objective of this research is to fabricate lipid nanoemulsions (LNs) for the co-delivery of PTX and docosahexaenoic acid (DHA) with folic acid (FA) decorating (PTX/DHA-FA-LNs), and investigate the anti-tumor activity of the...

Supplemental material, sj-xlsx-2-cat-10.1177_10760296221139258 for Establishment of a Predictive Model for Poor Prognosis of Incomplete Revascularization in Patients with Coronary Heart Disease and Multivessel Disease by Huan Lian, Zhuoyan Zhao, Kelin Ma, Zhenjiang Ding, Lixian Sun and Ying Zhang in Clinical and Applied Thrombosis/Hemostasis

Serum metabolite profiling in Duchenne muscular dystrophy (DMD) may enable discovery of valuable molecular markers for disease progression and treatment response. Serum samples from 51 DMD patients from a natural history study and 22 age-matched healthy volunteers were profiled using liquid chromatography coupled to mass spectrometry (LC-MS) for discovery of novel circulating serum metabolites associated with DMD. Fourteen metabolites were found significantly altered (1% false discovery rate) in their levels between DMD patients and healthy...

In December 2019, Wuhan, China suffered a serious outbreak of a novel coronavirus infectious disease (COVID) caused by novel severe acute respiratory syndrome-related coronavirus (SARS-CoV 2). To quickly identify the pathogen, we designed and screened primer sets, and established a sensitive and specific qRT-PCR assay for SARS-CoV 2; the lower limit of detection (LOD) was 15 (95% CI: 9.8–21) copies per reaction. We combined this qRT-PCR assay with an automatic integration system for nucleic acid...

Past and recent attempts at devising objective biomarkers for traumatic brain injury (TBI) in both blood and cerebrospinal fluid have focused on abundance measures of time-dependent proteins. Similar independent determinants would be most welcome in diagnosing the most common form of TBI, mild TBI (mTBI), which remains difficult to define and confirm based solely on clinical criteria. There are currently no consensus diagnostic measures that objectively define individuals as having sustained an acute mTBI. Plasma...

Spoken sentences are continuous streams of sound, without reliable acoustic cues to word boundaries. We have previously proposed that language learners identify words via an implicit statistical learning mechanism that computes transitional probabilities between syllables. Neuroimaging studies in healthy young adults associate this learning with left inferior frontal gyrus, left arcuate fasciculus, and bilateral striatum. Here, we test the effects of healthy aging and left hemisphere (LH) injury on statistical learning. Following 10-minute exposure to...

Electrocatalytic CO2 reduction to fuel is one of the important ways to solve energy and environmental problems. In this work, the preparation of hollow Aux-Cu2O electrocatalyst and the performance of electrocatalytic CO2 reduction to ethanol were studied. Hollow Cu2O nanospheres were prepared by a soft template method, and Aux-Cu2O composites were prepared by galvanic replacement. The characterization results of XRD and XPS reveal that Cu+ is the main chemical state of Cu in the catalysts....

Supplemental material, sj-docx-1-cat-10.1177_10760296221139258 for Establishment of a Predictive Model for Poor Prognosis of Incomplete Revascularization in Patients with Coronary Heart Disease and Multivessel Disease by Huan Lian, Zhuoyan Zhao, Kelin Ma, Zhenjiang Ding, Lixian Sun and Ying Zhang in Clinical and Applied Thrombosis/Hemostasis

Arsenic has been identified as a carcinogen, although the molecular mechanism underlying itscarcinogenesis has not been fully elucidated. To date, only a few studies have attempted to confirm a direct link between oxidative stress and the Warburg effect . This study demonstrated that 0.2 μmol/L As3+ induced the Warburg effect to contribute to abnormal proliferation of L-02 cells, that was mediated by upregulation of hexokinase 2 (HK2), a key enzyme in glycolysis. Further study indicated...

Supplemental material, sj-pdf-1-smm-10.1177_09622802221129861 for A general method for calculating power for GEE analysis of complete and incomplete stepped wedge cluster randomized trials by Ying Zhang, John S Preisser, Elizabeth L Turner, Paul J Rathouz, Mark Toles and Fan Li in Statistical Methods in Medical Research

Abstract Background Circular RNA (circRNA) is a type of stable non-coding RNA that modifies macrophage inflammation by sponging micro RNAs (miRNAs), binding to RNA-binding proteins, and undergoing translation into peptides. Activated M1 phenotype macrophages secrete matrix metalloproteinases to participate in softening of the cervix uteri to promote vaginal delivery. Methods In this study, the premature rupture of membranes (PROM) mouse model was used to analyze the role of macrophages in this process. Profiling of circRNAs...