5,518 Works

Additional file 4 of Fetuin-A alleviates neuroinflammation against traumatic brain injury-induced microglial necroptosis by regulating Nrf-2/HO-1 pathway

Pengzhan Zhao, Yutian Wei, Guangchi Sun, Lei Xu, Tian Wang, Yufei Tian, Honglu Chao, Yiming Tu & Jing Ji
Additional file 4: Fig. S4. A, B. Western blot analysis of Fetuin-A expression after transfected with shFetuin-A. GAPDH was used as the loading control. And bar graphs show the results of analysis (by band density analysis) of these proteins (n = 3). C. H&E staining of hemispheres sections (n = 3). D, E. Lesion volume (n = 5) and water content% (n = 5) were analyzed by statistical. F, G. Neuron death measured by TUNEL...

Additional file 6 of Fetuin-A alleviates neuroinflammation against traumatic brain injury-induced microglial necroptosis by regulating Nrf-2/HO-1 pathway

Pengzhan Zhao, Yutian Wei, Guangchi Sun, Lei Xu, Tian Wang, Yufei Tian, Honglu Chao, Yiming Tu & Jing Ji
Additional file 6: Fig. S6. PLX5622, which was specific CSF1R inhibitor, was able to achieve robust brain-wide microglia elimination. We fed PLX5622 to mice and tested its elimination efficiency. A, B. Immunofluorescence for Iba-1 in the hemisphere and statistical analysis of Iba-1. Scale bar is 100 μm (n = 3). Data are presented as the means ± SD; ***P < 0.001 vs. sham + Veh group, ###P < 0.001 vs. CCI + Veh group, and...

Fetuin-A alleviates neuroinflammation against traumatic brain injury-induced microglial necroptosis by regulating Nrf-2/HO-1 pathway

Pengzhan Zhao, Yutian Wei, Guangchi Sun, Lei Xu, Tian Wang, Yufei Tian, Honglu Chao, Yiming Tu & Jing Ji
Abstract Background The microglia-mediated inflammatory response is a vital mechanism of secondary damage following traumatic brain injury (TBI), but the underlying mechanism of microglial activation is unclear. Methods Controlled cortical impact (CCI) was induced in adult male C57BL/6J mice, and glutamate was used to construct a classical in vitro injury model in the primary microglia. Microglial activation was determined by western blot and immunostaining. The inflammatory factors were measured by enzyme-linked immunosorbent assay. The oxidative...

Additional file 3 of Fetuin-A alleviates neuroinflammation against traumatic brain injury-induced microglial necroptosis by regulating Nrf-2/HO-1 pathway

Pengzhan Zhao, Yutian Wei, Guangchi Sun, Lei Xu, Tian Wang, Yufei Tian, Honglu Chao, Yiming Tu & Jing Ji
Additional file 3: Fig. S3. A-B. Western blot analysis of Fetuin-A expression. GAPDH was used as the loading control. And bar graphs show the results of analysis (by band density analysis) of these proteins (n = 3). C. H&E staining of hemispheres sections (n = 3). D. water content% (n = 3) were analyzed by statistical. E–F. cell death measured by TUNEL staining. Scale bar is 100 μm (n = 3). Data are presented as...

Additional file 1 of iTRAQ-based proteomic analysis of Deinococcus radiodurans in response to 12C6+ heavy ion irradiation

Yuan Gao, Naikang Li, Yanxia Zhou, Zhenpeng Zhang, Yao Zhang, Pengcheng Fan, Hangfan Zhou, Tao Zhang, Lei Chang, Huiying Gao, Yanchang Li, Xianjiang Kang, Qiong Xie, Zhitang Lyu & Ping Xu
Additional file 1. Preparation for iTRAQ quantitative proteomics study.

Additional file 7 of iTRAQ-based proteomic analysis of Deinococcus radiodurans in response to 12C6+ heavy ion irradiation

Yuan Gao, Naikang Li, Yanxia Zhou, Zhenpeng Zhang, Yao Zhang, Pengcheng Fan, Hangfan Zhou, Tao Zhang, Lei Chang, Huiying Gao, Yanchang Li, Xianjiang Kang, Qiong Xie, Zhitang Lyu & Ping Xu
Additional file 7: Supplementary file1. Preparation for iTRAQ quantitative proteomics study, related to additional file 1

Additional file 4 of iTRAQ-based proteomic analysis of Deinococcus radiodurans in response to 12C6+ heavy ion irradiation

Yuan Gao, Naikang Li, Yanxia Zhou, Zhenpeng Zhang, Yao Zhang, Pengcheng Fan, Hangfan Zhou, Tao Zhang, Lei Chang, Huiying Gao, Yanchang Li, Xianjiang Kang, Qiong Xie, Zhitang Lyu & Ping Xu
Additional file 4. High correlation between the two technical replicates. The scatterplot of two technical replicates treated with 20/80/160 Gy heavy ion irradiation.

Additional file 5 of iTRAQ-based proteomic analysis of Deinococcus radiodurans in response to 12C6+ heavy ion irradiation

Yuan Gao, Naikang Li, Yanxia Zhou, Zhenpeng Zhang, Yao Zhang, Pengcheng Fan, Hangfan Zhou, Tao Zhang, Lei Chang, Huiying Gao, Yanchang Li, Xianjiang Kang, Qiong Xie, Zhitang Lyu & Ping Xu
Additional file 5. String analysis of 113 overlapped differential proteins in our dataset.

From Edge City to City?

Jennifer Day, Nicholas A. Phelps, Piret Veeroja & Xin Yang
This century’s planning challenge is one of retrofitting suburbia. As relatively compact suburban forms, edge cities may be the most amenable to retrofit. In the 30 years since the publication of Garreau’s Edge City, to what extent and in what ways have planning intentions changed to favor greater urbanity in edge cities? Across 10 indicators, we examined planning intentions covering 117 of 123 edge cities identified in 1991. Content analysis of plans revealed the mixed...

Effect of wounding and wound age on infection of canola cotyledons by Leptosphaeria maculans, interacting with leaf wetness

Shuanglong Huang, Chun Zhai, Zhongwei Zou, Fei Liu, Paula Parks, Linda Mcgregor, W. G. Dilantha Fernando & Gary Peng
Blackleg, caused by the fungus Leptosphaeria maculans, is an important disease of canola. Both ascospores and pycnidiospores of the fungus can infect intact leaves under conditions of extended leaf wetness, with infection then progressing further into the stem. In western Canada, spring is typically cool and dry and additional factors may be involved for successful infection. This study was designed to assess the effect of wounding and wound age on cotyledon infection, to evaluate the...

Italian translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

Nuno Costa, Petros Grivas, John A. Blake-Haskins, Bo Huang, Robert J. Laliberte, Daniel P. Petrylak, Jeanny B. Aragon-Ching, Joaquim Bellmunt, Cora N. Sternberg, Evgeny Kopyltsov, Norihiko Tsuchiya, Srikala S. Sridhar, Yohann Loriot, Anders Ullén, Wim Demey, Sinisa Radulović, Haralabos Kalofonos, Howard Gurney, Begoña P. Valderrama, Claudia Caserta, Eric Voog, Se Hoon Park & Thomas Powles
Italian translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

Italian translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

Nuno Costa, Petros Grivas, John A. Blake-Haskins, Bo Huang, Robert J. Laliberte, Daniel P. Petrylak, Jeanny B. Aragon-Ching, Joaquim Bellmunt, Cora N. Sternberg, Evgeny Kopyltsov, Norihiko Tsuchiya, Srikala S. Sridhar, Yohann Loriot, Anders Ullén, Wim Demey, Sinisa Radulović, Haralabos Kalofonos, Howard Gurney, Begoña P. Valderrama, Claudia Caserta, Eric Voog, Se Hoon Park & Thomas Powles
Italian translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

German translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

Petros Grivas, John A. Blake-Haskins, Nuno Costa, Bo Huang, Robert J. Laliberte, Daniel P. Petrylak, Jeanny B. Aragon-Ching, Joaquim Bellmunt, Cora N. Sternberg, Evgeny Kopyltsov, Norihiko Tsuchiya, Srikala S. Sridhar, Yohann Loriot, Anders Ullén, Wim Demey, Sinisa Radulović, Haralabos Kalofonos, Howard Gurney, Begoña P. Valderrama, Claudia Caserta, Eric Voog, Se-Hoon Park & Thomas Powles
German translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

German translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

Petros Grivas, John A. Blake-Haskins, Nuno Costa, Bo Huang, Robert J. Laliberte, Daniel P. Petrylak, Jeanny B. Aragon-Ching, Joaquim Bellmunt, Cora N. Sternberg, Evgeny Kopyltsov, Norihiko Tsuchiya, Srikala S. Sridhar, Yohann Loriot, Anders Ullén, Wim Demey, Sinisa Radulović, Haralabos Kalofonos, Howard Gurney, Begoña P. Valderrama, Claudia Caserta, Eric Voog, Se-Hoon Park & Thomas Powles
German translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

German translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

Petros Grivas, John A. Blake-Haskins, Nuno Costa, Bo Huang, Robert J. Laliberte, Daniel P. Petrylak, Jeanny B. Aragon-Ching, Joaquim Bellmunt, Cora N. Sternberg, Evgeny Kopyltsov, Norihiko Tsuchiya, Srikala S. Sridhar, Yohann Loriot, Anders Ullén, Wim Demey, Sinisa Radulović, Haralabos Kalofonos, Howard Gurney, Begoña P. Valderrama, Claudia Caserta, Eric Voog, Se-Hoon Park & Thomas Powles
German translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

German translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

Petros Grivas, John A. Blake-Haskins, Nuno Costa, Bo Huang, Robert J. Laliberte, Daniel P. Petrylak, Jeanny B. Aragon-Ching, Joaquim Bellmunt, Cora N. Sternberg, Evgeny Kopyltsov, Norihiko Tsuchiya, Srikala S. Sridhar, Yohann Loriot, Anders Ullén, Wim Demey, Sinisa Radulović, Haralabos Kalofonos, Howard Gurney, Begoña P. Valderrama, Claudia Caserta, Eric Voog, Se-Hoon Park & Thomas Powles
German translation of the JAVELIN Bladder 100 study: avelumab maintenance treatment for advanced urothelial cancer

Additional file 5 of Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway

Xiaopeng Shen, Meng Li, Chunguang Wang, Zhongxian Liu, Kun Wu, Ao Wang, Chao Bi, Shan Lu, Hongan Long & Guoping Zhu
Additional file 5: Fig. S5. The Wnt/β-Catenin pathway was affected by hypoxia. (A) The expression of canonical Wnts (Wnt3 and Wnt8a) and (B) Wnt/β-Catenin pathway downstream targets (Sp5 and Cdx1) was downregulated by hypoxia in AB2.2 mESCs undergoing differentiation. (C) The expression of canonical Wnts (Wnt3 and Wnt8a) and (D) Wnt/β-Catenin pathway downstream targets (Sp5 and Cdx1) was downregulated by hypoxia in R1 mESCs undergoing differentiation. (E-G) Expression of nuclear β-Catenin was significantly repressed, while...

Additional file 7 of Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway

Xiaopeng Shen, Meng Li, Chunguang Wang, Zhongxian Liu, Kun Wu, Ao Wang, Chao Bi, Shan Lu, Hongan Long & Guoping Zhu
Additional file 7: Fig. S7. The supplementary data of the effects of Hif-1α on the mesendoderm differentiation and Wnt/β-Catenin pathway. (A) The expression patterns of Egln1 in the differentiating AB2.2 mESCs under normoxia and hypoxia. (B) Doxycycline (Dox)-inducible overexpression of oxygen-resistant Hif-1α was verified by western blotting analysis. A Myc tag was added to the C-terminus of Hif-1α. (C) The mRNA expression of mesendoderm markers (T, Eomes, Mesp1, and Gsc) in AB2.2 mESCs with or...

Additional file 9 of Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway

Xiaopeng Shen, Meng Li, Chunguang Wang, Zhongxian Liu, Kun Wu, Ao Wang, Chao Bi, Shan Lu, Hongan Long & Guoping Zhu
Additional file 9: Table S1. List of primers used for RT-qPCR.

Additional file 8 of Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway

Xiaopeng Shen, Meng Li, Chunguang Wang, Zhongxian Liu, Kun Wu, Ao Wang, Chao Bi, Shan Lu, Hongan Long & Guoping Zhu
Additional file 8: Fig. S8. The supplementary analyses performed on the RNA-seq data of normoxia, hypoxia, control, and Hif-1α overexpression groups. The volcano plot showed the differentially expressed genes (DEGs) in (A) Hif-1α-OE_vs_Con and (B) HvsN_shHif-1α. |Log2(Fold Change)|>1 and adjusted P-value<0.05 were used as the cutoff criteria. (C) The GO terms of the DEGs upregulated by Hif-1α overexpression. GSEA of (D) apoptosis and (E) cell of AB2.2 mESCs on differentiation day 4 under hypoxia versus...

Additional file 2 of Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway

Xiaopeng Shen, Meng Li, Chunguang Wang, Zhongxian Liu, Kun Wu, Ao Wang, Chao Bi, Shan Lu, Hongan Long & Guoping Zhu
Additional file 2: Fig. S2. The effects of hypoxia on AB2.2 mESC differentiation performed with a high-serum differentiation medium. The differentiation in the high-serum differentiation medium was non-lineage-prone. In the differentiation performed with the high-serum medium, the expressions of (A) mesendoderm markers (T, Eomes, Mesp1, and Gsc) and (B) ectoderm markers (Pax6 and Nestin) were downregulated and upregulated by hypoxia, respectively. *, significant (P<0.05).

Additional file 11 of Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway

Xiaopeng Shen, Meng Li, Chunguang Wang, Zhongxian Liu, Kun Wu, Ao Wang, Chao Bi, Shan Lu, Hongan Long & Guoping Zhu
Additional file 11. A compressed file that included our original uncropped gel/blot images

Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway

Xiaopeng Shen, Meng Li, Chunguang Wang, Zhongxian Liu, Kun Wu, Ao Wang, Chao Bi, Shan Lu, Hongan Long & Guoping Zhu
Abstract Background Hypoxia naturally happens in embryogenesis and thus serves as an important environmental factor affecting embryo development. Hif-1α, an essential hypoxia response factor, was mostly considered to mediate or synergistically regulate the effect of hypoxia on stem cells. However, the function and relationship of hypoxia and Hif-1α in regulating mesendoderm differentiation remains controversial. Results We here discovered that hypoxia dramatically suppressed the mesendoderm differentiation and promoted the ectoderm differentiation of mouse embryonic stem cells...

Additional file 11 of Circular RNA circPVT1 promotes nasopharyngeal carcinoma metastasis via the β-TrCP/c-Myc/SRSF1 positive feedback loop

Yongzhen Mo, Yumin Wang, Yian Wang, Xiangying Deng, Qijia Yan, Chunmei Fan, Shuai Zhang, Shanshan Zhang, Zhaojian Gong, Lei Shi, Qianjin Liao, Can Guo, Yong Li, Guiyuan Li, Zhaoyang Zeng, Weihong Jiang, Wei Xiong & Bo Xiang
Additional file 11: Figure S2. circPVT1 promotes the migration and invasion of NPC in vitroand metastasis of NPCin vivo. A-B. The expression of circPVT1 were measured in CNE2 and 5-8F cells after transfection with the circPVT1 overexpression vector or circPVT1 siRNA. PVT1 expression was not affected in CNE2 and 5-8F cells after overexpresion or knockdown of circPVT. All experiments were repeated at least three times. Data were represented as mean ± SD. ***, p <...

Additional file 12 of Circular RNA circPVT1 promotes nasopharyngeal carcinoma metastasis via the β-TrCP/c-Myc/SRSF1 positive feedback loop

Yongzhen Mo, Yumin Wang, Yian Wang, Xiangying Deng, Qijia Yan, Chunmei Fan, Shuai Zhang, Shanshan Zhang, Zhaojian Gong, Lei Shi, Qianjin Liao, Can Guo, Yong Li, Guiyuan Li, Zhaoyang Zeng, Weihong Jiang, Wei Xiong & Bo Xiang
Additional file 12: Figure S3. circPVT1 promotes the migration and invasion of NPC cells through binding to β-TrCP. A. The secondary ion flow diagram of β-TrCP identified by mass spectrometry according to the peptide sequence (DFITALPAR) of β -TrCP.B. Schematic diagram of binding sites betweent circPVT1 and β-TrCP using the catRAPID website.The 230-280 nt of circPVT1 was predicted to bind to β-TrCP protein with high interaction score.C. The secondary structure of circPVT1 changed when 230-280...

Registration Year

  • 2022
    5,518

Resource Types

  • Text
    2,236
  • Collection
    1,292
  • Dataset
    1,118
  • Image
    767
  • Audiovisual
    57
  • Other
    43
  • Software
    5

Affiliations

  • Huazhong University of Science and Technology
    5,518
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    3,727
  • Shanghai Jiao Tong University
    3,239
  • Fudan University
    3,074
  • Zhejiang University
    3,068
  • Sichuan University
    2,836
  • Capital Medical University
    2,713
  • Nanjing Medical University
    2,602
  • Sun Yat-sen University
    2,565
  • Chinese Academy of Sciences
    2,454