10 Works

Additional file 1 of Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo & Sandeep Tamber
Additional file 1: Supplementary Figure 1. Relative abundances of species identified on retail chicken breast as determined by the Bruker Biotyper.

Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo & Sandeep Tamber
Abstract Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food. These methods rely on the detection of gene sequences to indicate the presence of viable bacteria. This indirect form of detection can be prone to experimental artefacts. Sample handling and processing are key sources of variation that require standard approaches. Extracting sufficient quantities of high quality DNA...

Additional file 1 of Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo & Sandeep Tamber
Additional file 1: Supplementary Figure 1. Relative abundances of species identified on retail chicken breast as determined by the Bruker Biotyper.

Development of SVA and FMD mRNA vaccines and expression of FMD structural proteins in an SVA infectious clone

Pablo Pineyro
The introduction of FMDV to the United States poses the single largest risk to the United States agricultural sector. The estimated 10-year impact on the United States economy following FMDV introduction is over $128 billion for the beef and pork sectors, $44 billion for the corn sector, $25 billion for the soybean sector, and a loss of approximately 1.5 million jobs40. Due to the high-risk wild-type, FMDV poses to the United States, wild-type FMDV work...

Additional file 2 of Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo & Sandeep Tamber
Additional file 2: Supplementary Figure 2. Microbial composition is altered by enrichment time.

Additional file 2 of Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo & Sandeep Tamber
Additional file 2: Supplementary Figure 2. Microbial composition is altered by enrichment time.

Data from: The genome of the yellow potato cyst nematode Globodera rostochiensis reveals insights into the basis of parasitism and virulence

Sebastian Eves-Van Den Akker, Dominik Laetsch, Peter Thorpe, Catherine Lilley, Etienne Danchin, Martine Da Rocha, Corinne Rancurel, Nancy Holroyd, James Cotton, Amir Szitenberg, Eric Grenier, Josselin Montarry, Benjamin Mimee, Marc-Olivier Duceppe, Ian Boyes, Jessica Marvin, Laura Jones, Hazijah Yusup, Joël Lafond-Lapalme, Magali Esquibet, Michael Sabeh, Michael Rott, Hein Overmars, Anna Finkers-Tomczak, Geert Smant … & John Jones
Background: The yellow potato cyst nematode Globodera rostochiensis is a devastating plant pathogen of global economic importance. This biotrophic parasite secretes effectors from pharyngeal glands, some of which were acquired by horizontal gene transfer, to manipulate host processes and promote parasitism. G. rostochiensis is classified into pathotypes with different plant resistance-breaking phenotypes. Results: We generate a high-quality genome assembly for G. rostochiensis pathotype Ro1, identify putative effectors and horizontal gene transfer events, map gene expression...

Data from: Demographic and spatiotemporal patterns of avian influenza infection at the continental scale, and in relation to annual life cycle of a migratory host

Rodolfo Nallar, Zsuzsanna Papp, Tasha Epp, Frederick A. Leighton, Seth R. Swafford, Thomas J. DeLiberto, Robert J. Dusek, Hon S. Ip, Jeffrey Hall, Johannes Berhane, Samantha E. J. Gibbs, Catherine Soos & Yohannes Berhane
Since the spread of highly pathogenic avian influenza (HPAI) H5N1 in the eastern hemisphere, numerous surveillance programs and studies have been undertaken to detect the occurrence, distribution, or spread of avian influenza viruses (AIV) in wild bird populations worldwide. To identify demographic determinants and spatiotemporal patterns of AIV infection in long distance migratory waterfowl in North America, we fitted generalized linear models with binominal distribution to analyze results from 13,574 blue-winged teal (Anas discors, BWTE)...

Data from: Mitotic recombination and rapid genome evolution in the invasive forest pathogen Phytophthora ramorumm

Angela L. Dale, Nicolas Feau, Sydney E. Everhart, Braham Dhillon, Barbara Wong, Julie Sheppard, Guillaume J. Bilodeau, Avneet Brar, Javier F. Tabima, Danyu Shen, Clive M. Brasier, Brett M. Tyler, Niklaus J. Grünwald & Richard C. Hamelin
Invasive alien species often have reduced genetic diversity and must adapt to new environments. Given the success of many invasions, this is sometimes called the genetic paradox of invasion. Phytophthora ramorum is invasive, limited to asexual reproduction within four lineages, and presumed clonal. NA1 is responsible for sudden oak death in the USA, NA1, NA2 and EU1 are responsible for ramorum blight in the USA and Canada and EU1 and EU2 are responsible for sudden...

Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo & Sandeep Tamber
Abstract Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food. These methods rely on the detection of gene sequences to indicate the presence of viable bacteria. This indirect form of detection can be prone to experimental artefacts. Sample handling and processing are key sources of variation that require standard approaches. Extracting sufficient quantities of high quality DNA...

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