An analysis method was developed for the separation and quantification of hesperidin, neohesperidin, neohesperidin dihydrochalcone and hesperetin by using HPLC-UV. Single factor experiments and Box-Behnken Designs were used to optimize separation of four flavonoids, in which a gradient elution method was adopted with 99% acetonitrile and 0.1% formic acid as mobile phases at a flow rate of 0.9 mL/min. A C18 column was used with a column temperature of 35 °C. LODs and LOQs were...

Supplementary Material 1

Additional file 1. The workflow and study design of the analysis steps.

Additional file 4.The expression level of immune checkpoints in high-risk and low-risk groups.

Additional file 5. Infiltration of gene expression, overall survival, mutation profile and the comparison of tumor infiltration levels in m6A-related genes with different somatic copy number alterations for LUAD and LUSC in TIMER2.0.

Additional file 8. The heatmap of correlation between the immune characteristics and the four hub DEMGs in LUAD and LUSC from TISID.

High-power, high-brightness laser lighting promotes new requirements for light-conversion materials, such as high thermal conductivity, high saturation threshold and compact encapsulation. In this paper, we designed and fabricated a novel composite structure ceramic including a 1.0×1.0 mm2 Al₂O₃-YAG:Ce ceramic and a φ=16.0 mm transparent YAG ceramic for the transmissive configuration in laser lighting. When pumped by blue laser from 0~60 W mm2, all the samples exhibited no luminous saturation phenomenon, and the 10.0 wt.%Al₂O₃-YAG:Ce/YAG composite...

Abstract CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR (CRISPR-Knock-ins and Random Inserts Searching PRotocol), a method to identify both off-target lesions and random insertions. CRISPR-KRISPR uses as little as 3.4 μg of genomic DNA; thus, it can be easily...

Abstract Background According to the Global Cancer Statistics in 2020, the incidence and mortality of colorectal cancer (CRC) rank third and second among all tumors. The disturbance of ubiquitination plays an important role in the initiation and development of CRC, but the ubiquitinome of CRC cells and the survival-relevant ubiquitination are poorly understood. Methods The ubiquitinome of CRC patients (n = 6) was characterized using our own data sets of proteomic and ubiquitin-proteomic examinations. Then,...

Supplemental material, sj-docx-1-hol-10.1177_09596836221131694 for Hydro-climatic aspects of prehistoric human dynamics in the drylands of the Asian interior by Kangkang Li, Xiaoguang Qin, Bing Xu, Zhiqiang Yin, Yong Wu, Guijin Mu, Dong Wei, Xiaohong Tian, Huiqiu Shao, Chunxue Wang, Hongjuan Jia, Wen Li, Haoze Song, Jiaqi Liu and Yingxin Jiao in The Holocene

The causes of prehistoric human migrations in the drylands of the Asian interior have long been debated among multidisciplinary scholars. The Bronze Age Xiaohe settlements (ca. 4000–3300 cal yr BP) are situated in the extremely arid Tarim Basin of northwest China and exemplify a societal collapse which included a long-distance movement along river catchments. Here, we present the results of stable carbon isotope values (δ13C) of archaeobotanical remains, sedimentary faces analysis from the Tarim River...

Additional file 1: Table S1. Composition and sequences of the synthetic DNA material sequins. Table S2. Characteristics of library construction kits. Table S3. 495 core microbial contaminations at the genus level.

Additional file 1: Table S1. Composition and sequences of the synthetic DNA material sequins. Table S2. Characteristics of library construction kits. Table S3. 495 core microbial contaminations at the genus level.

Additional file 3: Table S3. The classification, annotation and GO functional categorization of the PgMYB gene transcripts.

Additional file 3: Table S3. The classification, annotation and GO functional categorization of the PgMYB gene transcripts.

Abstract Background As the king of all herbs, the medicinal value of ginseng is self-evident. The perennial nature of ginseng causes its quality to be influenced by various factors, one of which is the soil environment. During plant growth and development, MYB transcription factors play an important role in responding to abiotic stresses and regulating the synthesis of secondary metabolites. However, there are relatively few reports on the MYB transcription factor family in Panax ginseng....

This report describes fossils recovered from Ganxian Cave in 2008 and 2018 by the Natural History Museum and Anthropology Museum of Guangxi. The cave sedimentary fill yielded rich mammalian fossils consisting mainly of isolated teeth of medium- to large-sized mammals (Primates, Proboscidea, Perissodactyla, Carnivora, Rodentia, Artiodactyla) of typical ‘Ailuropoda-Stegodon’ fauna, (e.g. Stegodon orientalis, Ailuropoda baconi, Pongo weidenreichi, Tapirus sinensis, Megatapirus augustus, Crocuta ultima), and more numerous extant species belonging to 28 taxa in total. The...

Additional file 1. Fig. S1. Optimized lambda selection (A) and feature selection (B) in Lasso regression. Fig. S2. Feature importance values in the Stacking model. Fig. S3. Schematic diagrams of the process for introducing missing values and interpolation in MCAR (A) and MNAR (B). Table S1. Parameter optimization results of KNN and MICE in MCAR. Table S2. Parameter optimization results of KNN and MICE in MNAR. Table S3. The optimized parameters of AE and RRLR....

Abstract Background Reproductive tract infection has become a major public health issue all over the world for its high and growing prevalence. It can cause adverse pregnancy outcomes in pregnant women and their foetuses. This study aimed to investigate the trends and risk factors of the prevalence of reproductive tract infections among women who prepared to conceive in the Chongqing Municipality (China) from 2012 to 2016. Methods A multi-center cross-sectional study was conducted between January...

This dataset contains data relates to the publication " Chemical Origin of Temperature-Dependent Phase Transition and Conductivity in Hydroxyapatite", by Wang et al., 2022. In this work, we use deep potential molecular dynamics simulation to study Temperature-Dependent Phase Transition and Conductivity in Hydroxyapatite. The deep neural network potential is trained on DFT calculations. This dataset contains the deep potential models and the DFT training data reported in the publication, the input files of vasp, lammps,...

Abstract Background Besides featured glucose consumption, recent studies reveal that cancer cells might prefer “addicting” specific energy substrates from the tumor microenvironment (TME); however, the underlying mechanisms remain unclear. Methods Fibroblast-specific long noncoding RNAs were screened using RNA-seq data of our NJLCC cohort, TCGA, and CCLE datasets. The expression and package of LINC01614 into exosomes were identified using flow cytometric sorting, fluorescence in situ hybridization (FISH), and quantitative reverse transcription polymerase chain reaction (RT-PCR). The...

Additional file 1: Table S1. Clinical improvements in rituximab cohort and control cohort

Additional file 1. On the orininal data of the study.

We previously described using a “Y” incision and rectangular patch to enlarge the aortic annulus by 2-3 valve sizes for aortic valve replacement (AVR) with bioprosthesis (1) or mechanical valve (2) in patients with trileaflet aortic valve (TAV). As we are becoming more experienced, we are able to enlarge the aortic annulus by 4 valve sizes routinely in either TAV or bicuspid aortic valve (BAV). In this video we used a case of TAV to...

Additional file 3. Table S3: Performance of three strategies.