70 Works

Data from: Trends and determinants of gastric bacterial colonization of preterm neonates in a NICU setting

Ketki S. Patel, Kavitha Konduru, Alok K. Patra, Dinesh S. Chandel, Pinaki Panigrahi & Ketki Patel
Background: Newborn gastrointestinal (GI) tract is considered sterile but rapidly acquires a diverse microbiota from its intimate environment. Early acquisition of a bacterial species in the upper GI tract may play a role in establishing the colonic microbiota. There is paucity of molecular data on the upper GI tract microbiota in preterm neonates. Methods: Gastric aspirates from 22 neonates with an average gestational age 27.7 weeks (±2.8), weighing 973.2 grams (±297.9) admitted to a neonatal...

sj-pdf-1-jfi-10.1177_0192513X211042843 – Supplemental Material for Interparental Conflict and Chinese Children’s Social Development

Fengjiao Xu, Xinyi Chen, Haiyan Xing & Hongmei Wang
Supplemental Material, sj-pdf-1-jfi-10.1177_0192513X211042843 for Interparental Conflict and Chinese Children’s Social Development by Fengjiao Xu, Xinyi Chen, Haiyan Xing and Hongmei Wang in Journal of Family Issues

CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice

Masayuki Tanaka, Keiko Yokoyama, Hideki Hayashi, Sanae Isaki, Kanae Kitatani, Ting Wang, Hisako Kawata, Hideyuki Matsuzawa, Channabasavaiah B. Gurumurthy, Hiromi Miura & Masato Ohtsuka
Abstract CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR (CRISPR-Knock-ins and Random Inserts Searching PRotocol), a method to identify both off-target lesions and random insertions. CRISPR-KRISPR uses as little as 3.4 μg of genomic DNA; thus, it can be easily...

Distinct roles of the Chlamydia trachomatis effectors TarP and TmeA in the regulation of formin and Arp2/3 during entry

Rey Carabeo & Matthew Romero
The obligate intracellular pathogen Chlamydia trachomatis manipulates the host actin cytoskeleton to assemble actin-rich structures that drive pathogen entry. The recent discovery of TmeA, which, like TarP, is an invasion-associated type III effector implicated in actin remodeling, raised questions regarding the nature of their functional interaction. Quantitative live-cell imaging of actin remodeling at invasion sites revealed differences in recruitment and turnover kinetics associated with the TarP and TmeA pathways, with the former accounting for most...

Additional file 2 of Incidence, clinical characteristics, risk factors and outcomes of patients with mixed Candida/bacterial bloodstream infections: a retrospective study

Li Zhong, Zhaohui Dong, Fengqi Liu, Haidong Li, Kankai Tang, Cheng Zheng, Lifang Wang, Kai Zhang, Jiachang Cai, Hongwei Zhou, Wei Cui, Yanqiu Gao & Gensheng Zhang
Additional file 2: Table S1. Comparison of vitro antifungal susceptibility of candida between mono-candidemia and mixed C/B-BSIs.

CC16 polymorphisms in asthma, asthma subtypes, and asthma control in adults from the Agricultural Lung Health Study

KC Gribben, AB Wyss, JA Poole, PA Farazi, C Wichman, M Richards-Barber, LE Beane Freeman, PK Henneberger, DM Umbach, SJ London, TD LeVan & Kelli C. Gribben
Abstract Background The club cell secretory protein (CC16) has anti-inflammatory and antioxidant effects and is a potential early biomarker of lung damage. The CC16 single nucleotide polymorphism (SNP) rs3741240 risk allele (A) has been inconsistently linked to asthma; other tagging SNPs in the gene have not been explored. The aim was to determine whether CC16 tagging polymorphisms are associated with adult asthma, asthma subtypes or asthma control in the Agricultural Lung Health Study (ALHS). Methods...

Supplemental tables: The association between health information technology and influenza vaccine uptake among adults with heart disease.docx

Onoriode Kesiena, Oluwaseun Famojuro & Temitope Olokunlade
Supplemental tables Table 1. Characteristics of individuals with heart disease who used the internet to look up health information Table 2. Characteristics of individuals with heart disease who used the internet to fill up prescriptions Table 3. Characteristics of individuals with heart disease who used the internet to schedule a medical appointment Table 4. Characteristics of individuals with heart disease who used an email to communicate with their healthcare provider

Additional file 1 of Current state and practice variation in the use of Meningitis/Encephalitis (ME) FilmArray panel in children

Prabi Rajbhandari, Nathaniel Goodrich, Aleisha M. Nabower, Miraides F. Brown, Maheswari Ekambaram, Jaclyn Eisenberg, Michael L. Forbes, Nathan Gollehon, Kimberly C. Martin, Russell McCulloh, Bryan Stone, Matt Tandy & Jessica Snowden
Supplementary Material 1

Additional file 1 of A novel dual epigenetic approach targeting BET proteins and HDACs in Group 3 (MYC-driven) Medulloblastoma

Matthew J. Kling, Varun Kesherwani, Nitish K. Mishra, Gracey Alexander, Erin M. McIntyre, Sutapa Ray, Kishore B. Challagundla, Shantaram S. Joshi, Don W. Coulter & Nagendra K. Chaturvedi
Additional file 1: Supplementary Table S1. JQ1 and panobinostat synergistically modulate gene expression. GSE analysis was performed using RNA-sequencing based differential gene expression in HD-MB03 cells 24 h after treatment with control (DMSO) solvent, 0.5 µM JQ1, 10 nM panobinostat, or the combination of JQ1 and panobinostat. GSE analysis generated enriched gene sets, confirming modulation of MYC/HDAC, cell cycle, apoptosis, hypoxia, EMT and stem cell target gene sets by JQ1 and panobinostat alone or in...

The challenges of testing metal and metal oxide nanoparticles in algal bioassays: titanium dioxide and gold nanoparticles as case studies

Nanna B. Hartmann, Christian Engelbrekt, Jingdong Zhang, Jens Ulstrup, Kresten Ole Kusk & Anders Baun
Aquatic toxicology of engineered nanoparticles is challenged by methodological difficulties stemming partly from highly dynamic and poorly understood behavior of nanoparticles in biological test systems. In this paper scientific and technical challenges of testing not readily soluble nanoparticles in standardised algal growth inhibition tests are highlighted with specific focus on biomass quantification methods. This is illustrated through tests with TiO2 and Au nanoparticles, for which cell-nanoparticle interactions and behavior was studied during incubation. Au NP...

Efficacy and safety of semaglutide for type 2 diabetes by race and ethnicity: a post hoc analysis of the SUSTAIN trials

Cyrus Desouza, Bertrand Cariou, Satish Garg, Nanna Lausvig, Andrea Navarria & Vivian Fonseca
Context: Variations in the prevalence and aetiology of type 2 diabetes (T2D) across race and ethnicity may affect treatment responses. Semaglutide is a glucagon-like peptide-1 (GLP-1) analog approved for once–weekly, subcutaneous (s.c.) treatment of T2D. Objective: To compare semaglutide efficacy and safety in race and ethnicity subgroups across the SUSTAIN trials. Design: Post hoc analysis of data from phase 3 randomized SUSTAIN 1–5 and 7 (pooled) and SUSTAIN 6 trials. Participants: 3,074 subjects (SUSTAIN 1–5...

Acute hypertensive response in patients with acute intracerebral hemorrhage: A narrative review

Maximiliano Hawkes & Alejandro Rabinstein
Objective: To review the role of the acute hypertensive response in patients with intracerebral hemorrhage, current treatment options and areas for further research. Methods: Systematic review of the literature to assess 1) Frequency of acute hypertensive response in intracerebral hemorrhage 2) Consequences of acute hypertensive response in clinical outcomes 3) Acute hypertensive response and secondary brain injury: hematoma expansion and perihematomal edema 4) Vascular autoregulation, safety data side effects of acute antihypertensive treatment, and 5)...

Multiplex-Immunoflourescent Staining of Rapid Autopsy Samples from Human Pancreatic Cancer

Michael Hollingsworth
Sequential staining of human tissue samples collected from a pancreatic rapid autopsy program using Multiplex-Immunofluorescence. Samples are taken from primary tumors at the pancreas. Characterization focuses on immune infiltration.

Additional file 1 of CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice

Masayuki Tanaka, Keiko Yokoyama, Hideki Hayashi, Sanae Isaki, Kanae Kitatani, Ting Wang, Hisako Kawata, Hideyuki Matsuzawa, Channabasavaiah B. Gurumurthy, Hiromi Miura & Masato Ohtsuka
Additional file 1. This includes fourteen supplementary figures, four supplementary tables and supplementary text.

Current state and practice variation in the use of Meningitis/Encephalitis (ME) FilmArray panel in children

Prabi Rajbhandari, Nathaniel Goodrich, Aleisha M. Nabower, Miraides F. Brown, Maheswari Ekambaram, Jaclyn Eisenberg, Michael L. Forbes, Nathan Gollehon, Kimberly C. Martin, Russell McCulloh, Bryan Stone, Matt Tandy & Jessica Snowden
Abstract Background The Meningitis/Encephalitis FilmArray® Panel (ME panel) was approved by the U.S. Food and Drug Administration in 2015 and provides rapid results when assessing patients with suspected meningitis or encephalitis. These patients are evaluated by various subspecialties including pediatric hospital medicine (PHM), pediatric emergency medicine (PEM), pediatric infectious diseases, and pediatric intensive care unit (PICU) physicians. The objective of this study was to evaluate the current use of the ME panel and describe the...

Additional file 2 of A novel dual epigenetic approach targeting BET proteins and HDACs in Group 3 (MYC-driven) Medulloblastoma

Matthew J. Kling, Varun Kesherwani, Nitish K. Mishra, Gracey Alexander, Erin M. McIntyre, Sutapa Ray, Kishore B. Challagundla, Shantaram S. Joshi, Don W. Coulter & Nagendra K. Chaturvedi
Additional file 2: Supplementary Table S2. The top 50 genes most significantly downregulated by the combined JQ1-PAN treatment in HD-MB03 cells.

Additional file 2 of A novel dual epigenetic approach targeting BET proteins and HDACs in Group 3 (MYC-driven) Medulloblastoma

Matthew J. Kling, Varun Kesherwani, Nitish K. Mishra, Gracey Alexander, Erin M. McIntyre, Sutapa Ray, Kishore B. Challagundla, Shantaram S. Joshi, Don W. Coulter & Nagendra K. Chaturvedi
Additional file 2: Supplementary Table S2. The top 50 genes most significantly downregulated by the combined JQ1-PAN treatment in HD-MB03 cells.

A novel dual epigenetic approach targeting BET proteins and HDACs in Group 3 (MYC-driven) Medulloblastoma

Matthew J. Kling, Varun Kesherwani, Nitish K. Mishra, Gracey Alexander, Erin M. McIntyre, Sutapa Ray, Kishore B. Challagundla, Shantaram S. Joshi, Don W. Coulter & Nagendra K. Chaturvedi
Abstract Background Medulloblastoma (MB) patients with MYC oncogene amplification or overexpression exhibit extremely poor clinical outcomes and respond poorly to current therapies. Epigenetic deregulation is very common in MYC-driven MB. The bromodomain extra-terminal (BET) proteins and histone deacetylases (HDACs) are epigenetic regulators of MYC transcription and its associated tumorigenic programs. This study aimed to investigate the therapeutic potential of inhibiting the BET proteins and HDACs together in MB. Methods Using clinically relevant BET inhibitors (JQ1...

Additional file 1 of Household disinfection practices by women living in Egypt during the 2020 COVID-19 lockdown and the association of information sources and suspected bleach toxicity

Maha Farid, Rania Talaat, Valerie Pacino, Hyo Jung Tak & Wael ElRayes
Additional file 1: Supp. Fig 1. The disinfectants used by participants since the beginning of the lockdown. Supp. Fig 2. The responses to the question: if you used bleach for disinfection during the lockdown, did you mix with any other substances?. Supp. Fig 3. The responses to the question: How did you use the cleaning products and disinfectants in your household during the lockdown?. Supp. Fig 4. The responses to the question: Did you use...

Measurement error correction in mediation analysis under the additive hazards model

Ying Yan, Mingchen Ren & Alexander de Leon
In the disciplines of medicine, biology and social science, causal mediation analysis is widely used to examine the effect of an exposure on an outcome through different causal pathways. Recently, there is rapid development of mediation analysis with survival data. An early work of Lange and Hansen proposed to identify the direct and indirect causal effects under the additive hazards model with a single mediator. However, inaccurate measurements of mediators and confounders may lead to...

Data from: Trends in malaria in Odisha, India—an analysis of the 2003-2013 time-series data from the National Vector Borne Disease Control Program

Ashirbad Pradhan, Anita Anasuya, Madan Mohan Pradhan, A. K. Kavitha, Priyanka Kar, Krushna Chandra Sahoo, Pinaki Panigrahi, Ambarish Dutta & Kavitha AK
Background: Although Odisha is the largest contributor to the malaria burden in India, no systematic study has examined its malaria trends. Hence, the spatio-temporal trends in malaria in Odisha were assessed against the backdrop of the various anti-malaria strategies implemented in the state. Methods: Using the district-wise malaria incidence and blood examination data (2003-2013) from the National Vector Borne Disease Control Program, blood examination-adjusted time-trends in malaria incidence were estimated and predicted for 2003-2013 and...

Data from: Unique features of germline variation in five Egyptian familial breast cancer families revealed by exome sequencing

Yeong C. Kim, Amr S. Soliman, Jian Cui, Mohamed Ramadan, Ahmed Hablas, Mohamed Abouelhoda, Nehal Hussien, Ola Ahmed, Nabawy Zekri, Ibrahim A. Seifeldin, San Ming Wang & Abdel-Rahman Nabawy Zekri
Genetic predisposition increases the risk of familial breast cancer. Recent studies indicate that genetic predisposition for familial breast cancer can be ethnic-specific. However, current knowledge of genetic predisposition for the disease is predominantly derived from Western populations. Using this existing information as the sole reference to judge the predisposition in non-Western populations is not adequate and can potentially lead to misdiagnosis. Efforts are required to collect genetic predisposition from non-Western populations. The Egyptian population has...

Novel Genetic Algorithm (GA) based hybrid machine learning-pedotransfer Function (ML-PTF) for prediction of spatial pattern of saturated hydraulic conductivity

Vijay Kumar Singh, Kanhu Charan Panda, Atish Sagar, Nadhir Al-Ansari, Huan-Feng Duan, Pradosh Kumar Paramaguru, Dinesh Kumar Vishwakarma, Ashish Kumar, Devendra Kumar, P. S. Kashyap, R. M. Singh & Ahmed Elbeltagi
Saturated hydraulic conductivity (Ks) is an important soil characteristic that controls water moves through the soil. On the other hand, its measurement is difficult, time-consuming, and expensive; hence Pedotransfer Functions (PTFs) are commonly used for its estimation. Despite significant development over the years, the PTFs showed poor performance in predicting Ks. Using Genetic Algorithm (GA), two hybrid Machine Learning based PTFs (ML-PTF), i.e. a combination of GA with Multilayer Perceptron (MLP-GA) and Support Vector Machine...

Additional file 1 of Household disinfection practices by women living in Egypt during the 2020 COVID-19 lockdown and the association of information sources and suspected bleach toxicity

Maha Farid, Rania Talaat, Valerie Pacino, Hyo Jung Tak & Wael ElRayes
Additional file 1: Supp. Fig 1. The disinfectants used by participants since the beginning of the lockdown. Supp. Fig 2. The responses to the question: if you used bleach for disinfection during the lockdown, did you mix with any other substances?. Supp. Fig 3. The responses to the question: How did you use the cleaning products and disinfectants in your household during the lockdown?. Supp. Fig 4. The responses to the question: Did you use...

Household disinfection practices by women living in Egypt during the 2020 COVID-19 lockdown and the association of information sources and suspected bleach toxicity

Maha Farid, Rania Talaat, Valerie Pacino, Hyo Jung Tak & Wael ElRayes
Abstract Introduction The spread of contradictory health information was a hallmark of the early COVID-19 pandemic. Because of a limited understanding of the disease, its mode of transmission, and its pathogenicity, the public turned to easily accessible and familiar sources of information. Some of these sources included wrong or incomplete information that could increase health risks and incidents of toxicity due to improper information about the usage of disinfectants. The objective of this study was...

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Affiliations

  • University of Nebraska Medical Center
    70
  • Huazhong University of Science and Technology
    14
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    10
  • Nanjing Medical University
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  • Colorado State University
    7
  • North West Agriculture and Forestry University
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  • West China Hospital of Sichuan University
    6
  • Ya'an Polytechnic College
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  • Jiangxi Agricultural University
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  • Xuan Wu Hospital of the Capital Medical University
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