Optimized culture systems for the preimplatation ICR mouse embryos with wide range of EDTA concentrations

Yun Seok Heo, Seema Thapa & Seung Hee Kang
In this study, in vitro preimplantation embryo culture media especially for outbred stock mice (Institute of Cancer Research (ICR)) were optimized with different concentrations of Ethylenediaminetetraacetic acid (EDTA). A plot with embryo development rates against EDTA concentrations ranging from 0 to 500µM showed a unique pattern with two characteristic peaks. 200µM was adopted as an optimal concentration of EDTA. The optimized media were also evaluated with two culture systems; conventional large volume culture system (1ml)...

Registration Year

  • 2021
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Resource Types

  • Dataset
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Affiliations

  • Keimyung University
    1
  • Keimyung University Dongsan Medical Center
    1