8 Works

Emergent properties as by-products of prebiotic evolution of aminoacylation ribozymes

Evan Janzen, Yuning Shen, Alberto Vazquez-Salazar, Ziwei Liu, Celia Blanco, Josh Kenchel & Irene Chen
The emergence of the genetic code was a major transition in the evolution from a prebiotic RNA world to the earliest modern cells. A prominent feature of the standard genetic code is error minimization, or the tendency of mutations to be unusually conservative in preserving biophysical features of the amino acid. While error minimization is often assumed to result from natural selection, it has also been speculated that error minimization may be a by-product of...

Data from: Neuropeptide signalling shapes feeding and reproductive behaviours in male C. elegans

Matthew Gadenne, Iris Hardege, Eviatar Yemini, Djordji Suleski, Paris Jaggers, Isabel Beets, William Schafer & Yee Lian Chew
Sexual dimorphism occurs where different sexes of the same species display differences in characteristics not limited to reproduction. For the nematode Caenorhabditis elegans, in which the complete neuroanatomy has been solved for both hermaphrodites and males, sexually dimorphic features have been observed both in terms of the number of neurons and in synaptic connectivity. In addition, male behaviours, such as food-leaving to prioritise searching for mates, have been attributed to neuropeptides released from sex-shared or...

Deep sequencing data for document titled: Rolling circle RNA synthesis catalysed by RNA

Emil L. Kristoffersen
RNA-catalysed RNA replication is widely considered a key step in the emergence of life’s first genetic system. However, RNA replication can be impeded by the extraordinary stability of duplex RNA products, which must be dissociated for re-initiation of the next replication cycle. Here we have explored rolling circle synthesis (RCS) as a potential solution to this strand separation problem. RCS on small circular RNAs - as indicated by molecular dynamics simulations - induces a progressive...

Encapsulation of ribozymes inside model protocells leads to faster evolutionary adaptation

Yei-Chen Lai, Ziwei Liu & Irene Chen
Functional biomolecules, such as RNA, encapsulated inside a protocellular membrane are believed to have comprised a very early, critical stage in the evolution of life, since membrane vesicles allow selective permeability and create a unit of selection enabling cooperative phenotypes. The biophysical environment inside a protocell would differ fundamentally from bulk solution, due to the microscopic confinement. However, the effect of the encapsulated environment on ribozyme evolution has not been previously studied experimentally. Here we...

Imaging the onset of oscillatory signaling dynamics during mouse embryo gastrulation

Henning J Falk, Takehito Tomita, Gregor Moenke, Katie McDole & Alexander Aulehla
A fundamental requirement for embryonic development is the coordination of signaling activities in space and time. A notable example in vertebrate embryos is found during somitogenesis, where gene expression oscillations linked to the segmentation clock are synchronized across cells in the presomitic mesoderm (PSM) and result in tissue-level wave patterns. To examine their onset during mouse embryo development, we studied the dynamics of the segmentation clock gene Lfng during gastrulation. To this end, we established...

Data supplement to: Quality control of image sensors using gaseous tritium light sources

David McFadden, Brad Amos & Rainer Heintzmann
In the article “Quality Control of Image Sensors using Gaseous Tritium Light Sources” (https://doi.org/10.1098/rsta.2021.0130) we propose a practical method for radiometrically calibrating cameras using widely available gaseous tritium light sources (betalights). This dataset includes all the recorded data along with the scripts necessary to reproduce the results and figures.

Data from: Random-sequence genetic oligomer pools display an innate potential for ligation and recombination

Hannes Mutschler, Alexander I. Taylor, Benjamin T. Porebski, Alice Lightowlers, Gillian Houlihan, Mikhail Abramov, Piet Herdewijn & Philipp Holliger
Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification mediated by dedicated recombinase enzymes. Here, we describe a pervasive non-enzymatic capacity for recombination (and ligation) in random-sequence genetic oligomer pools. Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids). While DNA, ANA and HNA pools proved...

Data from: EFHC1, implicated in juvenile myoclonic epilepsy, functions at the cilium and synapse to modulate dopamine signaling

Catrina M. Loucks, Kwangjin Park, Denise S. Walker, Andrea H. McEwan, Tiffany A. Timbers, Evan L. Ardiel, Laura J. Grundy, Chunmei Li, Jacque-Lynne Johnson, Julie Kennedy, Oliver E. Blacque, William R. Schafer, Catharine H. Rankin & Michel R. Leroux
Neurons throughout the mammalian brain possess non-motile cilia, organelles with varied functions in sensory physiology and cellular signaling, yet their roles in these neurons are poorly understood. To shed light into their functions, we studied EFHC1, an evolutionarily conserved protein required for motile cilia function and linked to a common form of inherited epilepsy in humans, juvenile myoclonic epilepsy (JME). We demonstrate that C. elegans EFHC1 functions within specialized non-motile mechanosensory cilia, where it regulates...

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  • 2022
  • 2021
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  • MRC Laboratory of Molecular Biology
  • University of California Los Angeles
  • KU Leuven
  • University of Wollongong
  • Flinders University
  • Simon Fraser University
  • Leibniz Institute of Photonic Technology
  • University of California, Santa Barbara
  • European Molecular Biology Laboratory
  • University of British Columbia