Atherosclerosis is the leading cause of coronary heart disease. In recent years, circ_0029589 (circCHFR) has been found to be associated with atherosclerosis development. However, the molecular mechanism of circCHFR action in atherosclerosis development is unknown. This study was aimed to investigate the function and action mechanism of circCHFR in atherosclerosis development. An atherosclerosis cell model was created by exposing human vascular endothelial cells (HUVECs) to oxidized low-density lipoprotein. The expression of circCHFR, microRNA(miR)-15b-5p, growth arrest...

The occurrence of fungal infection seriously affects the survival and life quality of transplanted patients. The accurate diagnosis is of particular importance in the early stage of infection. To develop a novel diagnostic method for this kind of patient, we established a post-transplant immunosuppressed mice model with fungus inoculation and collected their peripheral blood at specific time points after infection. After screening by microarray, differentially expressed miRNAs and lncRNAs were selected and homologously analyzed with...

The occurrence of fungal infection seriously affects the survival and life quality of transplanted patients. The accurate diagnosis is of particular importance in the early stage of infection. To develop a novel diagnostic method for this kind of patient, we established a post-transplant immunosuppressed mice model with fungus inoculation and collected their peripheral blood at specific time points after infection. After screening by microarray, differentially expressed miRNAs and lncRNAs were selected and homologously analyzed with...

Graft failure (GF) is an intractable complication of transplantation, which can severely affect the efficacy of the graft; however, the characteristics, incidence, and risk factors of primary GF have not been well described. This study aimed to analyze the risk factors and outcomes of primary GF to swiftly identify high-risk patients for GF. We performed a case-control study with a case-control ratio of 1:4 with 869 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT)...

Long non-coding RNA (lncRNA) ZFAS1 (zinc finger antisense 1) was demonstrated to play critical roles in various cancer progression. However, the functions of ZFAS in cervical cancers (CC) are unclear. Human CC cell lines were used for in vitro experiments. RT-qPCR (Real Time Quantitative PCR) was performed to detect the expression of ZFAS1, microRNA-190a-3p (miR-190a-3p) and Kruppel-like factor 6 (KLF6). Cell proliferation, invasion and migration assays were used to investigate biological behaviors of CC cells...

The North Qaidam Orogenic Belt (NQOB) is characterized by widespread Palaeozoic magmatism and high to ultrahigh-pressure metamorphism that provides important information regarding the continental crust growth and tectonic evolution in the North Qaidam. Our work focuses on the geology, geochronology, whole-rock geochemistry, and Sr–Nd isotopy of granitoids in the Tanjianshan area in the northern NQOB. The results indicate that the 486–469 Ma adakitic and TTG-like granitoids originated from the partial melting of Middle–Late Mesoproterozoic lower...

Macroautophagy/autophagy is a conserved cellular mechanism to degrade unneeded cytoplasmic proteins and organelles to recycle their components, and it is critical for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Whereas autophagy is essential for early development of embryos, no information exists regarding its functions during the transition from naive-to-primed pluripotency. Here, by using an in vitro transition model of ESCs to epiblast-like cells (EpiLCs), we find that dynamic changes in ATG7-dependent autophagy are...

Pot and field trials were conducted to investigate Cd uptake and phytoremediation efficiency of two Brassica napus cultivars (QY-1 and SYH) with applied water-soluble chitosan (WSC, Pot: 0, 2% and 4%; Field: 0 and 10 g·m−2) grown in Cd-contaminated soils. The results from the pot and field trials generally showed that WSC treatments significantly increased Cd concentrations in shoot and root tissues by 33.77–159.71% (except for SYH/JY) and 7.42–168.71% of two B. napus cultivars compared...

A variety of disturbances such as starvation, organelle damage, heat stress, hypoxia and pathogen infection can influence the autophagic process. However, how the macroautophagy/autophagy machinery is regulated intrinsically by structural damage of the cell remains largely unknown. In this work, we utilized the C. elegans epidermis as the model to address this question. Our results showed that structural damage by mechanical wounding exerted proximal inhibitory effect and distant promotional effect on autophagy within the same...

Clear cell renal cell carcinoma, the most common type of renal cancer, is associated with poor survival. Ubiquitin-specific peptidase 2 regulates the molecular mechanisms of cancer cells. However, its mechanism in clear cell renal cell carcinoma remains unclear. Quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry were performed to assess ubiquitin-specific peptidase 2 expression in human clear cell renal cell carcinoma samples. Ubiquitin-specific peptidase 2 was weakly expressed in clear cell renal cell...

The EIF3 gene family is essential in controlling translation initiation during the cell cycle. The significance of the EIF3 subunits as prognostic markers and therapeutic targets in breast cancer is not yet clear. We analyzed the expression of EIF3 subunits in breast cancer on the GEPIA and Oncomine databases and compared their expression in breast cancer and normal tissues using BRCA data downloaded from TCGA. Then we performed clinical survival analysis on the Kaplan–Meier Plotter...

The EIF3 gene family is essential in controlling translation initiation during the cell cycle. The significance of the EIF3 subunits as prognostic markers and therapeutic targets in breast cancer is not yet clear. We analyzed the expression of EIF3 subunits in breast cancer on the GEPIA and Oncomine databases and compared their expression in breast cancer and normal tissues using BRCA data downloaded from TCGA. Then we performed clinical survival analysis on the Kaplan–Meier Plotter...

In this paper, cyclone separator is proposed for the first time to separate particles according to density, and it is applied to the recovery of lithium iron phosphate (LFP) from spent lithium battery materials for the first time. The effect of the flow field on the motion of injected particles in a cyclone separator is studied by means of computational fluid dynamics (CFD). It is found that there is a classification phenomenon caused by the...

In this paper, cyclone separator is proposed for the first time to separate particles according to density, and it is applied to the recovery of lithium iron phosphate (LFP) from spent lithium battery materials for the first time. The effect of the flow field on the motion of injected particles in a cyclone separator is studied by means of computational fluid dynamics (CFD). It is found that there is a classification phenomenon caused by the...

A series of new acylthiourea derivatives were synthesized and characterized. The results of preliminary herbicidal activity tests showed that most compounds had good herbicidal activity against Digitaria adscendens and Amaranthus retroflexus, especially for compounds 4b (N-(2-chloro-4-methylsulfonylbenzoyl)-N’-(2-chlorophenyl) thiourea) and 4f (N-(2-chloro-4-methylsulfonylbenzoyl)-N’-(2,3-dichlorophenyl) thiourea). In vivo acetylhydroxylate synthase (AHAS) enzyme activity inhibition tests showed that all compounds had inhibitory activities against the enzyme at a concentration of 100 mg·L−1, with the highest inhibition rate up to 37.05% (compound...

Background. Far-field electrograms from superior vena cava (SVC) can be present in right superior pulmonary vein (RSPV) after pulmonary vein (PV) isolation. Objectives. To analyze the characteristics of far-field SVC potentials in RSPV after PV isolation and the local anatomy difference between patients with and without the potentials. Methods. Patients undergoing PV isolation were retrospectively reviewed, contrast-enhanced computed tomography (CT) was performed before procedure for observing the anatomical relationship between RSPV and SVC. The prevalence...

Activated sludge is a widely applied wastewater treatment process that mainly uses suspended microbial flocs to remove pollutants in wastewater. With the characteristics of high biomass content and high microbial diversity, activated sludge plays an important role in pollutant removal and contains various functional microorganisms as a valuable pool of various useful microbial resources. However, the majority of microorganisms in activated sludge have not been isolated, which substantially limits the improvement of treatment efficiency and...

Context: The high expression of long non-coding RNA deleted in lymphocytic leukaemia 2 (lncRNA DLEU2) has been confirmed in gastric cancer (GC). Objective: However, the detailed mechanism concerning its involvement in GC remained unclear, which we aimed to explore in this study. Materials and methods: LncRNA DLEU2 expression in GC was estimated by bioinformatic analysis, and the relationship between the expression of DLEU2 and the clinicopathological characteristics of patients with GC was performed. qRT-PCR was...

Context: The high expression of long non-coding RNA deleted in lymphocytic leukaemia 2 (lncRNA DLEU2) has been confirmed in gastric cancer (GC). Objective: However, the detailed mechanism concerning its involvement in GC remained unclear, which we aimed to explore in this study. Materials and methods: LncRNA DLEU2 expression in GC was estimated by bioinformatic analysis, and the relationship between the expression of DLEU2 and the clinicopathological characteristics of patients with GC was performed. qRT-PCR was...

Drought plays a prominent role in affecting ecosystem stability and ecosystem productivity. Based on eddy covariance and climatic observations during 2012–2020, the Fisher discriminant analysis method was employed to accurately detect drought occurrences. Furthermore, the ecosystem water sensitivity and its resistance to drought were quantified to evaluate the ecosystem stability. The results showed that the alpine meadow suffered drought most frequently at the beginning of the growing seasons. However, drought during the peak growing seasons...

When the number of strongly correlated electrons becomes larger, the single-reference coupled-cluster (CC) CCSD, CCSDT, etc. hierarchy displays an erratic behaviour, while traditional multi-reference approaches may no longer be applicable due to enormous dimensionalities of the underlying model spaces. These difficulties can be alleviated by the approximate coupled-pair (ACP) theories, in which selected (T2)2 diagrams in the CCSD amplitude equations are removed, but there is no generally accepted and robust way of incorporating connected triply...

Fluorine-doped graphene supported PbO (F-rGO/PbO) composite was prepared through a facile water bath method to study an improvement strategy for its catalytic performance in thermal decomposition of ammonium perchlorate (AP). The characterization results showed that the PbO with the size of 20 ∼ 30 nm are highly dispersed on the surface of F-doped graphene (F-rGO). Compared with other samples, the F-rGO/PbO exhibited superior catalytic performance, as 3 wt% of catalyst added in AP, the peak...

Strawberry has high nutritional and economic value and is an important horticultural crop widely cultivated worldwide. Strawberry anthracnose is the most harmful disease in strawberry production, which causes huge production losses every year. Mining disease resistance genes and breeding high-quality strawberry varieties are the fundamental way to solve the problem of strawberry anthracnose. In this study, transcriptome analysis and disease resistance-related genes mining were targeted on strawberry anthracnose. The main results are as follows: (1)...

Blueberries are rich in anthocyanins, which are plant-specific secondary metabolites that are a good source of plant nutrients. Although anthocyanins have been investigated, there is a lack of comprehensive detailed research that includes large numbers of blueberry cultivars, and the anthocyanin compositions of some cultivars are still unknown. Consequently, we determined the anthocyanin compositions of 62 cultivars using ultra-performance liquid chromatography–mass spectrometry (UPLC–MS). In total, we identified 30 anthocyanins derived from five kinds of anthocyanidin....

Model averaging has a rich history dating from its use for combining forecasts from time-series models (Bates and Granger, 1969) and presents a compelling alternative to model selection methods. We propose a frequentist model averaging procedure defined over categorical regression splines (Ma, Racine, and Yang, 2015) that allows for mixed-data predictors, as well as non-nested and heteroskedastic candidate models. We demonstrate the asymptotic optimality of the proposed model averaging estimator, and develop a post-averaging inference...