Neuropathic pain is a common dose-limiting side effect of oxaliplatin, which hampers the effective treatment of tumors. Here, we found that upregulation of transcription factor NFATc2 decreased the expression of Beclin-1, a critical molecule in autophagy, in the spinal dorsal horn, and contributed to neuropathic pain following oxaliplatin treatment. Meanwhile, manipulating autophagy levels by intrathecal injection of rapamycin (RAPA) or 3-methyladenine (3-MA) differentially altered mechanical allodynia in oxaliplatin-treated or naïve rats. Utilizing chromatin immunoprecipitation-sequencing (ChIP-seq)...

The chlorination titanium blast furnace slag belongs to industrial waste, but after grinding, they can be used to prepare cementing fluids to realize the secondary utilization of solid waste resources. As a common additive, fluid loss additives are mostly used in oil well cement systems, and there is still a lot of room for development when applied to chlorination titanium blast furnace slag systems. In this paper, the AMPS/DN-1/DMC/AA tetramer fluid loss additive was synthesized...

Supplemental material, sj-docx-1-tct-10.1177_15330338231157156 for MicroRNA-592 Inhibits the Growth of Ovarian Cancer Cells by Targeting ERBB3 by Qiong Jin, Ning Zhang, Yang Zhan, Xiaohong Xu, Chao Han, Hui Zhao and Xiaodi Hu, Hongbo Tang, Yumei Wu in Technology in Cancer Research & Treatment

Additional file 2.

Matrine is one of the major alkaloids extracted from Sophora flavescens Ait of the traditional Chinese medicine, was the main chemical ingredient of compounds of Kushen injection. The Matrine is considered as a promising therapeutic agent for curing nonsmall cell lung cancer (NSCLC), used either alone or combined with chemotherapeutic agents. In the present study, we focused on the possible roles of Matrine exerted on the self-renewal ability of stem-like cells of the NSCLC group,...

Supplemental material, sj-pdf-3-jcb-10.1177_0271678X231165653 for H3K9 trimethylation dictates neuronal ferroptosis through repressing Tfr1 by Ting Lan, Liye Hu, Tingting Sun, Xuechun Wang, Zhongnan Xiao, Danmin Shen, Weihua Wu, Zhaoli Luo, Chao Wei, Xiaotong Wang, Meng Liu, Yi Guo, Liyong Wang, Yamei Wang, Yabin Lu, Yan Yu, Fei Yang, Chenguang Zhang and Qian Li in Journal of Cerebral Blood Flow & Metabolism

Spontaneous intracerebral hemorrhage (ICH) is a devastating disease with high morbidity and mortality worldwide. We have previously shown that ferroptosis contributes to neuronal loss in ICH mice. The overload of iron and dysfunction of glutathione peroxidase 4 (GPx4) promote neuronal ferroptosis post-ICH. However, how epigenetic regulatory mechanisms affect the ferroptotic neurons in ICH remains unclear. In the current study, hemin was used to induce ferroptosis in N2A and SK-N-SH neuronal cells to mimic ICH. The...

Cotton (Gossypium hirsutum) is an important economic crop, so it is of great significance to analyze its molecular mechanisms of resistance to Verticillium Wilt. The WRKY gene family encodes transcription factors (TFs) involved in plant resistance to biotic and abiotic stress. Here, we identified 17 group III WRKY genes in cotton based on the cotton genome project. The phylogenetic relationship and evolutionary of WRKY Group III TFs in Arabidopsis thaliana and G. hirsutum showed that...

1. Design of off-axis reflective system for filamentation;2. Parameters of optimized free-form surface

Femtosecond laser filamentation propagating tens of meters to several kilometers with high intensity in the atmosphere has been demonstrated as a powerful tool for remote sensing. In contrast to the refractive systems, the reflective optical systems possess a variety of advantages including broad bandwidth, large aperture, light weight and low energy loss. However, astigmatic aberration is inevitably introduced by off-axis reflective mirrors. It can greatly affect the filament quality, which is critical for exciting and...

Understanding the biological rhythms that influence young adult health is vital because the combination of biological changes and a circadian phase delay lead to young adults being at high risk of circadian misalignment. We have previously established a self-rating of biological rhythm disorder for adolescents (SBRDA). However, we did not externally validate the SBRDA against objective measures of biological rhythms such as dim light melatonin onset (DLMO)—the gold standard of the endogenous circadian phase. The...

Oral cancer is a common life-threatening malignancy having high mortality and morbidity rates. During the treatment process, individuals unavoidably experience severe side effects. It is essential to develop safer and more effective strategies. Currently, extracellular vesicles (EVs) and biomimetic nanoparticles are nanomedicines with long-term blood circulation and lower off-target toxicity that orchestrate immune responses and accumulate specifically in tumor sites. EVs create a synergetic effect by encapsulating drugs and collaborating with naturally loaded elements in...

Oral cancer is a common life-threatening malignancy having high mortality and morbidity rates. During the treatment process, individuals unavoidably experience severe side effects. It is essential to develop safer and more effective strategies. Currently, extracellular vesicles (EVs) and biomimetic nanoparticles are nanomedicines with long-term blood circulation and lower off-target toxicity that orchestrate immune responses and accumulate specifically in tumor sites. EVs create a synergetic effect by encapsulating drugs and collaborating with naturally loaded elements in...

Abstract Background Elucidating the phylogenetic relationships within species-rich genera is essential but challenging, especially when lineages are assumed to have been going through radiation events. Mahonia Nutt. (Berberidaceae) is a genus with cosmopolitan distribution, comprising approximately 100 species, two of which are known as Caulis Mahoniae (M. bealei and M. fortunei) with crucial pharmacological significance in Chinese herbal medicine. Mahonia is a taxonomically challenging genus, and intrageneric phylogenetic relationships still need to be explored using...

Additional file 2.

Supplementary Material 1

Additional file 3: Table S3. GO Gene Ontology analysis of co-expressed protein-coding genes with all candidate lncRNAs.

Additional file 5: Fig. S2. Expression levels of lncRNAs and PCgenes in sunflower endosperm.

Abstract Background Long non-coding RNAs (lncRNAs), as important regulators, play important roles in plant growth and development. The expression and epigenetic regulation of lncRNAs remain uncharacterized generally in plant seeds, especially in the transient endosperm of the dicotyledons. Results In this study, we identified 11,840 candidate lncRNAs in 12 day-after-pollination sunflower endosperm by analyzing RNA-seq data. These lncRNAs were evenly distributed in all chromosomes and had specific features that were distinct from mRNAs including tissue-specificity...

Additional file 2: Figure S2. Community heatmap showing the top 50 genera.

Abstract In some regions, integrated rice-fish farms have been developed to balance the needs of aquaculture wastewater discharge and rice field irrigation. In this type of aqua-agriculture system, soil is irrigated with aquaculture wastewater, and intestinal bacteria in cultured fish species likely impact soil bacteria through irrigation. However, little is known about the relationship between soil bacteria and intestinal bacteria in some carp species commonly co-cultured in some Asian regions. Therefore, we co-cultured five carp...

Additional file 1: Figure S1. Pie chart shows the number of differentially expressed lncRNAs in each category.

Additional file 2: Table S1. The characteristics of methylation-driven lncRNAs.

Additional file 4: Figure S3. Volcano plot showing the log2 fold change of 517 significantly differentially expressed miRNAs in CRC patients from the TCGA database.

Additional file 1: Fig. S1. Visualization of alignment of M. bealei RC601 and six outgroups. Mahonia bealei RC601 was used as a reference sequence. Blue represents coding regions, pink represents non-coding regions and gray arrows points at genes. Fig. S2. Comparison of the LSC, IR and SSC boundary regions of plastomes of M. bealei RC601 and six outgroups. Fig. S3. Structural variation between plastomes of ten species of Mahonia revealed by Mauve. Fig. S4. Phylogenetic...