1. Design of off-axis reflective system for filamentation;2. Parameters of optimized free-form surface

Abstract Background Cox proportional hazards regression models and machine learning models are widely used for predicting the risk of dementia. Existing comparisons of these models have mostly been based on empirical datasets and have yielded mixed results. This study examines the accuracy of various machine learning and of the Cox regression models for predicting time-to-event outcomes using Monte Carlo simulation in people with mild cognitive impairment (MCI). Methods The predictive accuracy of nine time-to-event regression...

Abstract Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis. As a potential zoonotic pathogen, MAP also seriously threatens human health and social security. At present, long non-coding RNA (lncRNA) has attracted wide attention as an useful biomarker in various diseases. Therefore, our study analyzed the lncRNA expression profiles and lncRNA-mRNA regulatory network of MAP infected bovine monocytes-macrophages and uninfected bovine cells by high-throughput sequencing. A total of 4641 differentially expressed lncRNAs genes...

Abstract Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis. As a potential zoonotic pathogen, MAP also seriously threatens human health and social security. At present, long non-coding RNA (lncRNA) has attracted wide attention as an useful biomarker in various diseases. Therefore, our study analyzed the lncRNA expression profiles and lncRNA-mRNA regulatory network of MAP infected bovine monocytes-macrophages and uninfected bovine cells by high-throughput sequencing. A total of 4641 differentially expressed lncRNAs genes...

The experiment and results.

Additional file 3: Figure S2. Correlation (P values derive from Spearman’s correlation) between DNA methylation and the expression of LIFR-AS1 in CRC samples.

Additional file 3: Figure S2. Correlation (P values derive from Spearman’s correlation) between DNA methylation and the expression of LIFR-AS1 in CRC samples.

With doses simulating those received by spent nuclear fuel (SNF) facilities, gamma-ray (γ-ray) irradiation experiments were conducted on hexagonal zirconium. γ-ray irradiation–induced crystalline defects were observed in the hexagonal zirconium and characterized. Monte Carlo calculations combined with Transmission Electron Microscopy characterizations revealed that the energy of primary knock-on atoms could reach the threshold energy (Ed) of zirconium atoms and result in dislocation loops within the α-zirconium grains. The influence of γ dose on dislocation loop...

Steroid-induced osteonecrosis of the femoral head (SONFH) is a progressive disease that leads to an increased disability rate. This study aimed to ascertain biomarkers, infiltrating immune cells, and therapeutic drugs for SONFH. The gene expression profile of the GSE123568 dataset was downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified using the NetworkAnalyst platform. Functional enrichment, protein-protein interaction network (PPI), and module analyses were performed using Metascape tools. An...

Supplemental material, sj-xlsx-1-imr-10.1177_03000605221141312 for CCK regulates osteogenic differentiation through TNFα/NF-κB in peri-implantitis by LongHang Chou, YaTing Chang, KaiWen Lan, Meng Liu, YuKun Lu, XiaoLei Li, PeiRu Li and Yue Xu in Journal of International Medical Research

Supplemental Material for NFATc2-dependent epigenetic downregulation of the TSC2/Beclin-1 pathway is involved in neuropathic pain induced by oxaliplatin by Meng Liu, Jing-Wen Ma, De-Xing Luo, Guan-Xi Liu, Ting Xu, Wen-Jun Xin, Su-Yan Lin, and Zhen-Yu Li in Molecular Pain

Neuropathic pain is a common dose-limiting side effect of oxaliplatin, which hampers the effective treatment of tumors. Here, we found that upregulation of transcription factor NFATc2 decreased the expression of Beclin-1, a critical molecule in autophagy, in the spinal dorsal horn, and contributed to neuropathic pain following oxaliplatin treatment. Meanwhile, manipulating autophagy levels by intrathecal injection of rapamycin (RAPA) or 3-methyladenine (3-MA) differentially altered mechanical allodynia in oxaliplatin-treated or naïve rats. Utilizing chromatin immunoprecipitation-sequencing (ChIP-seq)...

RNA editing generates genetic diversity in mammals by altering amino acid sequences, miRNA targeting site sequences, influencing the stability of targeted RNAs, and causing changes in gene expression. However, the extent to which RNA editing affect gene expression via modifying miRNA binding site remains unexplored. Here, we first profiled the dynamic A-to-I RNA editome across tissues of Duroc and Luchuan pigs. The RNA editing events at the miRNA binding sites were generated. The biological function...

Supplemental material, sj-xls-1-jcb-10.1177_0271678X231165653 for H3K9 trimethylation dictates neuronal ferroptosis through repressing Tfr1 by Ting Lan, Liye Hu, Tingting Sun, Xuechun Wang, Zhongnan Xiao, Danmin Shen, Weihua Wu, Zhaoli Luo, Chao Wei, Xiaotong Wang, Meng Liu, Yi Guo, Liyong Wang, Yamei Wang, Yabin Lu, Yan Yu, Fei Yang, Chenguang Zhang and Qian Li in Journal of Cerebral Blood Flow & Metabolism

Supplemental material, sj-pdf-4-jcb-10.1177_0271678X231165653 for H3K9 trimethylation dictates neuronal ferroptosis through repressing Tfr1 by Ting Lan, Liye Hu, Tingting Sun, Xuechun Wang, Zhongnan Xiao, Danmin Shen, Weihua Wu, Zhaoli Luo, Chao Wei, Xiaotong Wang, Meng Liu, Yi Guo, Liyong Wang, Yamei Wang, Yabin Lu, Yan Yu, Fei Yang, Chenguang Zhang and Qian Li in Journal of Cerebral Blood Flow & Metabolism

Supplemental material, sj-xls-2-jcb-10.1177_0271678X231165653 for H3K9 trimethylation dictates neuronal ferroptosis through repressing Tfr1 by Ting Lan, Liye Hu, Tingting Sun, Xuechun Wang, Zhongnan Xiao, Danmin Shen, Weihua Wu, Zhaoli Luo, Chao Wei, Xiaotong Wang, Meng Liu, Yi Guo, Liyong Wang, Yamei Wang, Yabin Lu, Yan Yu, Fei Yang, Chenguang Zhang and Qian Li in Journal of Cerebral Blood Flow & Metabolism

Cotton (Gossypium hirsutum) is an important economic crop, so it is of great significance to analyze its molecular mechanisms of resistance to Verticillium Wilt. The WRKY gene family encodes transcription factors (TFs) involved in plant resistance to biotic and abiotic stress. Here, we identified 17 group III WRKY genes in cotton based on the cotton genome project. The phylogenetic relationship and evolutionary of WRKY Group III TFs in Arabidopsis thaliana and G. hirsutum showed that...

The aim of this study was to develop a gambogic acid-loaded mixed micelles (GA-M) system, using Kolliphor HS15 and lecithin, for enhancement of oral bioavailability. GA-M was prepared using the thin film hydration method, and particle size and zeta potential indexes were used to determine the optimized formulation was optimized with taking particle size, zeta potential as indexes. The optimal GA-M system had a mean particle size in the nanometer range (87.22 ± 0.68 nm)...

TableS1. Clinical data of ovarian cancer patients from TCGA and ICGC.
TableS2.Identified DNA driver methylation (DNAme) in TCGA, ICGC and GSE146556.
TableS3. The correlation between DNAme and corresponding mRNA expression in TCGA, ICGC and GSE146556. TableS4.Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of DNAme-associated mRNA in TCGA.
TableS5. Univariate Cox regression analysis of DNAme-associated mRNA. (P < 0.05)
TableS6. Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of...

ObjectivePeri-implantitis is characterized by peri-implant mucositis and alveolar bone resorption. This study investigated cholecystokinin (CCK) expression and the mechanism underlying its involvement in peri-implantitis.MethodsmRNA sequencing was performed using the Gene Expression Omnibus database GSE106090. Human bone marrow mesenchymal stem cells (hBMSCs) were pretreated with various concentrations of CCK (0, 10, 30, or 100 nM) for 1 hour before induction in osteogenic differentiation medium for 2 weeks. Alkaline phosphatase (ALP) activity was determined, and the cells...

Additional file 4: Fig. S1. Expressed lncRNAs in two crosses.

Additional file 6: Fig. S3. DNA methylation profiles of long non-coding RNAs (lncRNAs) and protein-coding genes (PCgenes) in sunflower endosperm from SY1.

Additional file 9: Fig. S5. Identification of imprinted long non-coding RNAs (lncRNAs) in sunflower endosperm at 12 DAP.

Additional file 9: Fig. S5. Identification of imprinted long non-coding RNAs (lncRNAs) in sunflower endosperm at 12 DAP.

Abstract Background Long non-coding RNAs (lncRNAs), as important regulators, play important roles in plant growth and development. The expression and epigenetic regulation of lncRNAs remain uncharacterized generally in plant seeds, especially in the transient endosperm of the dicotyledons. Results In this study, we identified 11,840 candidate lncRNAs in 12 day-after-pollination sunflower endosperm by analyzing RNA-seq data. These lncRNAs were evenly distributed in all chromosomes and had specific features that were distinct from mRNAs including tissue-specificity...