2 Works

Data from: The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways

Qingqing Zhu, Lu Song, Guangdun Peng, Na Sun, Jun Chen, Ting Zhang, Nengyin Sheng, Wei Tang, Cheng Qian, Yunbo Qiao, Ke Tang, Jing-Dong Han, Jinsong Li, Naihe Jing & Jing-Dong Jackie Han
The neural fate commitment of pluripotent stem cells requires the repression of extrinsic inhibitory signals and the activation of intrinsic positive transcription factors. However, how these two events are integrated to ensure appropriate neural conversion remains unclear. Here, we showed that Pou3f1 is essential for the neural differentiation of mouse embryonic stem cells (ESCs), specifically during the transition from epiblast stem cells (EpiSCs) to neural progenitor cells (NPCs). Chimeric analysis showed that Pou3f1 knockdown leads...

Data from: Forward genetic screening for regulators involved in cholesterol synthesis using validation-based insertional mutagenesis

Wei Jiang, Jing-Jie Tang, Hong-Hua Miao, Yu-Xiu Qu, Jie Qin, Jie Xu, Jinbo Yang, Bo-Liang Li & Bao-Liang Song
Somatic cell genetics is a powerful approach for unraveling the regulatory mechanism of cholesterol metabolism. However, it is difficult to identify the mutant gene(s) due to cells are usually mutagenized chemically or physically. To identify important genes controlling cholesterol biosynthesis, an unbiased forward genetics approach named validation-based insertional mutagenesis (VBIM) system was used to isolate and characterize the 25-hydroxycholesterol (25-HC)-resistant and SR-12813-resisitant mutants. Here we report that five mutant cell lines were isolated. Among which,...

Registration Year

  • 2014
    2

Resource Types

  • Dataset
    2

Affiliations

  • Shanghai Institutes for Biological Sciences
    2
  • Institute of Biochemistry
    2
  • Sichuan University
    1
  • Lanzhou University
    1
  • Wuhan University
    1
  • Chinese Academy of Sciences
    1
  • Nanchang University
    1