Abstract Background Limited progress in terms of an effective treatment for spinal cord injury (SCI) emphasizes the urgent need for novel therapies. As a vital central nervous system component, the resident astrocytes play crucial roles in regulating recovery after SCI. In this study, recovery after SCI was compared following the transplantation of either A1 or A2 astrocytes. A1 astrocytes are harmful as they upregulate the neurotoxic classical complement cascade genes. Conversely, A2 astrocytes are characterized...

Abstract Background Mitochondrial dysfunction is an important pathogenic event in acute kidney injury (AKI). GCN5L1 is a specific acetyltransferase in mitochondria, which regulates glucose and fatty acid metabolism. However, the role of GCN5L1 in mitochondrial dysfunction and the pathogenesis of ischemic AKI are not fully understood. Methods The protein level of GCN5L1 was detected by western blot assay. Acetylated proteomics was used to explore the level of acetylated TFAM. Duolink proximity ligation assay and co-immunoprecipitation...

Abstract Background The effects of traumatic brain injury (TBI) can include physical disability and even death. The development of effective therapies to promote neurological recovery is still a challenging problem. 3D-printed biomaterials are considered to have a promising future in TBI repair. The injury-preconditioned secretome derived from human umbilical cord blood mesenchymal stem cells showed better stability in neurological recovery after TBI. Therefore, it is reasonable to assume that a biological scaffold loaded with an...

Additional file 1: Fig. S1. Typical representative images of TEM (A–C) and HE staining (D–F) of 3D-CH-IB-ST. G Degradation of 3D-CH-IB-ST in rats over 8 weeks. Fig. S2. A–D Representative graphs of phase contrast microscopy (A) and immunofluorescence staining (Nestin) (B–D) of cultured NSCs

Additional file 4: Fig. S4. The co-expression network of the PgHDZ gene transcripts expressed in four-year-old plant roots of 42 cultivars. (a) The co-expression network of the PgHDZ gene transcripts constructed at p ≤ 5.0E-02. (b) Ten clusters of the co-expression network. (c and d) The co-expression network formation tendency of the PgHDZ gene transcripts presented by the number of nodes (c) and the number of edges (d). (e and f) Statistics of the co-expression...

Additional file 7: Table S3. The nucleotide sequences of the PgHDZ gene transcripts identified from ginseng cv. Damaya and their subfamilies.

Abstract Background Children's injuries from traffic accidents have been identified as a global public health issue. Child restraint system (CRS) is a useful tool for lowering the risk of injury to children. Nevertheless, CRS usage is really low in China. The goal of the current study was to investigate the use of CRS after the legislation revised in China and to explore the influencing factors based on Information, Motivation, and Behavioral Skills model (IMB). Methods...

Additional file 2. The genes in the module 1 were extracted and the correlation of clustered genes with modules was calculated.

Additional file 7. Prediction on the target genes of 13 candidate miRNAs (hsa-mir-28, hsa-mir-744, hsa-let-7i, hsa-mir-584, hsa-mir-423, hsa-mir-1249, hsa-mir-1275, hsa-mir-25, hsa-mir-146b, hsa-mir-345, hsa-mir-1237 and hsa-mir-1301.

Additional file 1: Supplementary Table S1. The sequences of promoter PRKU6a and promoter PRKU6b. Supplementary Table S2. The sequence of codon-optimized Cas9-NLS-6*His. Table S3. The sequence of sgRNA of RKCrtYB used in the present study. Supplementary Table S4. Primers used in the present study.

Additional file 2: Table S2. Lung cancer cell lines and their culture condition.

Additional file 4: Table S4. List of index of organs in each group.

Abstract Background Small-cell lung cancer (SCLC) is a high malignant and high energy-consuming type of lung cancer. Total coumarins of Pileostegia tomentella (TCPT) from a traditional folk medicine of Yao minority, is a potential anti-cancer mixture against SCLC, but the pharmacological and molecular mechanism of TCPT remains largely unknown. Methods Screening of viability inhibition of TCPT among 7 cell lines were conducted by using CCK-8 assays. Anti-proliferative activities of TCPT in SCLC were observed by...

Additional file 4: Table S4. KEGG pathway analysis of DEGs.

Additional file 4: Table S4. KEGG pathway analysis of DEGs.

Additional file 5: Table S5. Differential expression of phytohormone-related genes in plants after CYVCV infection.

Additional file 5: Table S5. Differential expression of phytohormone-related genes in plants after CYVCV infection.

Additional file 4: Table S1 Neurological scoring system.

Abstract Background The first wave of COVID-19 in Calgary, Alberta accelerated the integration of primary care with the province’s centrally managed health system. This integration aimed to deliver wraparound in-community patient care through two interventions that combined to create the COVID-19 Integrated Pathway (CIP). The CIP’s interventions were: 1) a data sharing platform that ensured COVID-19 test results were directly available to family physicians (FPs), and 2) a clinical algorithm that supported FPs in delivering...

Additional file 1: Raw data of all figures.

Abstract Background Knee osteoarthritis (KOA) is one of the most common degenerative diseases. Its development is closely related to cartilage injury and subchondral bone remodeling homeostasis. In the present study, we combined icariin-conditioned serum (ICS) with thiolated chitosan (CSSH), a material widely used in tissue engineering for cartilage repair, to demonstrate its effect on the repair of cartilage damage and abnormal subchondral remodeling. Methods New Zealand rabbits were undergoing surgery for cartilage defect, and joint...

Additional file 2: Dataset S1. The stage-specific pACRs.

Additional file 2. m6A peaks related genes in H and NH groups.

Abstract Background Heat stress has a substantial negative economic impact on the dairy industry. N6-methyladenosine (m6A) is the most common internal RNA modification in eukaryotes and plays a key role in regulating heat stress response in animals. In dairy cows, however, this modification remains largely unexplored. Therefore, we examined the effects of heat stress on the m6A modification and gene expression in bovine mammary epithelial cells to elucidate the mechanism of heat stress response. In...

Additional file 2: Figure S2. Variant allele frequency and copy number change in three patient cohorts. a Variant allele frequency (VAF) of EGFR and MET TKD mutations in baseline patients. b The copy number of MET amplification before and after MET-TKI treatment. c VAF of MET exon 14 skipping alterations (METex14) before and after MET-TKI treatment. d VAF of EGFR and MET TKD mutations in patients before and after EGFR-TKI treatment.