A stereo interplanting system with foxtail millet beneath chestnut trees is an effective planting method to raise the utilization of land in chestnut orchards, increase yields and improve quality of chestnut nuts. Consequently, exploration of genes involved in shade tolerance response in foxtail millet and breeding shade-tolerant varieties have become urgent issues. In this study, RNA-seq of leaf samples from two shade-tolerant varieties and three shade-intolerant varieties of foxtail millet at the booting stage was...

Interstitial lung disease (ILD) events associated with anti-human epidermal growth factor receptor 2 (HER2) antibody-drug conjugates (ADCs) have aroused wide attention. In meta-analysis, we systematically reviewed literatures, and the outcomes were the proportion and risk of ILD related to anti-HER2 ADCs. A disproportionality analysis based on data from VigiBase was conducted to characterize the main features of anti-HER2 ADC-related ILD/pneumonitis. Two hundred and forty-five all-grade and 47 grade ≥ 3 ILD events with the proportion...

Interstitial lung disease (ILD) events associated with anti-human epidermal growth factor receptor 2 (HER2) antibody-drug conjugates (ADCs) have aroused wide attention. In meta-analysis, we systematically reviewed literatures, and the outcomes were the proportion and risk of ILD related to anti-HER2 ADCs. A disproportionality analysis based on data from VigiBase was conducted to characterize the main features of anti-HER2 ADC-related ILD/pneumonitis. Two hundred and forty-five all-grade and 47 grade ≥ 3 ILD events with the proportion...

Additional file 1: Demographic, breast cancer epidemiological and screening factors of the female populations in China.

A stereo interplanting system with foxtail millet beneath chestnut trees is an effective planting method to raise the utilization of land in chestnut orchards, increase yields and improve quality of chestnut nuts. Consequently, exploration of genes involved in shade tolerance response in foxtail millet and breeding shade-tolerant varieties have become urgent issues. In this study, RNA-seq of leaf samples from two shade-tolerant varieties and three shade-intolerant varieties of foxtail millet at the booting stage was...

Additional file 14. Figure S14. Reclustering analysis of tumor-infiltrating NK cells. (a) T-distributed stochastic neighbor embedding (t-SNE) plot for NK cells. (b) t-SNE plot for the treatment group-specific distribution of NK cell subclusters. (c) Violin plots showing NK cell subcluster-specific gene profiles. (d) Histogram representing the proportion of NK cells subcluster in each group. (e-f) Violin plots showing the levels of Cd69 and Tnf of NK cell subclusters. (g) Heatmap depicting the results of GSEA...

Additional file 2. Figure S2: Flow cytometry for daughter CD4+ T cell in one-way mixed lymphocyte reaction (MLR). Stimulating cells were BMDCs derived from BALB/c mice while responding cells were spleen cells from C57BL/6 in the MLR assays. The mixed cells with YM101 or control antibodies were cultured for four days. On day 5, cells were collected for CFSE dilution assay.

Additional file 2. Figure S2: Flow cytometry for daughter CD4+ T cell in one-way mixed lymphocyte reaction (MLR). Stimulating cells were BMDCs derived from BALB/c mice while responding cells were spleen cells from C57BL/6 in the MLR assays. The mixed cells with YM101 or control antibodies were cultured for four days. On day 5, cells were collected for CFSE dilution assay.

Additional file 6. Figure S6: The effect of the combination therapy on T cell infiltration and peritumoral collagen deposition in the EMT-6 model. (a) Immunofluorescent staining showing T cells in tumor margin and center. (b) Picrosirius red staining showing picrosirius red staining.

Additional file 7. Figure S7: The FACS gating strategies for the B16 model.

Additional file 7. Figure S7: The FACS gating strategies for the B16 model.

Abstract Background Non-inflamed tumors, including immune-excluded and immune-desert tumors, are commonly resistant to anti-PD-1/PD-L1 (α-PD-1/PD-L1) therapy. Our previous study reported the potent antitumor activity of anti-TGF-β/PD-L1 bispecific antibody YM101 in immune-excluded tumors. However, YM101 had limited antitumor activity in immune-desert models. MSA-2 is a novel oral stimulator of interferon genes (STING) agonist, which activates the innate immune system and may synergize with YM101 in overcoming immunotherapy resistance. Methods The dose-dependent effect of MSA-2 on STING...

Additional file 2: Fig S1. (A) Echocardiography shows transposition of great arteries and ventricular septal defect in the patient JM0087 (F-2: II-1). (B) Chest X-ray shows dextrocardia in the patient JM0529 (F-4:II-1) at the age of 2 years

Additional file 4: Table S2. Probes sequences targeting NUP188 and PDXDC1 in MLPA. GAPDH served as a internal control

Additional file 4: Table S2. Probes sequences targeting NUP188 and PDXDC1 in MLPA. GAPDH served as a internal control

Additional file 7: Table S3. Primers used for Sanger sequencing for TTC21B and TTC12 variants that identified from WES analyzes

Additional file 7: Table S3. Primers used for Sanger sequencing for TTC21B and TTC12 variants that identified from WES analyzes

Additional file 2: Fig. S1. The phenotypes of WT, Ov-2 and Ov-3 lines. (a) The growing of WT, Ov-2 and Ov-3 lines under control condition. (b) The growing of WT, Ov-2 and Ov-3 lines under RBSDV infection conditions.

Additional file 1.

Abstract Background Malnutrition is common in critically ill patients, but nutrition status in critically ill patients with acute kidney injury (AKI) has been poorly studied. Our study aimed to investigate the relationship between malnutrition risk and the occurrence and prognosis of AKI in elderly patients in the intensive care unit (ICU). Methods Data were extracted from the Beijing Acute Kidney Injury Trial (BAKIT). A total of 1873 elderly patients were included and compared according to...

Additional file 1: Figure S1. Correlation between gene significance and related module membership. A. The correlation between gene significance and module membership in the gray module. B. The correlation between gene significance and module membership in the blue module. *p < 0.05, **p < 0.01, ***p < 0.001.

Additional file 3: Figure S3. Identification of candidate hub genes to predict prognosis for AML patients. A. Partial likelihood deviance for different numbers of variables revealed by the LASSO regression model. Red dots represent the partial likelihood deviance values. Gray lines represent the partial likelihood deviance ± standard error. B. Fourteen candidate genes with minimum lambda values were obtained by LASSO regression with tenfold cross-validation. C. Candidate genes were filtrated from SVM-RFE algorithms. D. Five...

Additional file 4: Figure S4. The expression levels of DLC1, NFIB, DENND5B, TANC2, and ELAVL4 in AML patients with different survival status. Scatter plots of the five gene’s expression level in AML patients with different survival status based on GEO dataset. *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations: GEO, Gene Expression Omnibus.

Additional file 5: Figure S5. Kaplan-Meier survival curve according to the DLC1 expression level for 48 AML patients in our center. Kaplan-Meier plots was used to visualize the overall survival probability of 48 patients based on the expression of DLC1 at diagnosis in our center.

Additional file 6: Table S1. Clinical characteristics of 48 AML patients. Table S2. Basic information for the included GEO databases.