Additional file 2: Clinical stage-specific disability weights and distribution of breast cancer in females in China.

Additional file 4: Age-specific number of females and standardized life expectancy

Additional file 1. Supplementary Figures. Figure S1 shows Aβ labelling in z-stack image volumes of AD and control retinal punches, scanning through the layers from RNFL to INL; Figure S2 shows additional examples of GS and GFAP double-labelling at higher power; Figure S3 shows quantitative methods used to analyse macroglia; Figure S4 illustrates double labelling.

Additional file 3: Table S2. The CDSs of ZmABCB family.

Additional file 4: Table S3. The primer sequences for the qRT-PCR.

Additional file 12. Figure S12. Histogram representing the proportion of clusters in each group.

Additional file 13. Figure S13. Bubble plots showing the results of DEG functional enrichment analysis using GO gene sets in T cells, NK cells, and cDCs.

Additional file 14. Figure S14. Reclustering analysis of tumor-infiltrating NK cells. (a) T-distributed stochastic neighbor embedding (t-SNE) plot for NK cells. (b) t-SNE plot for the treatment group-specific distribution of NK cell subclusters. (c) Violin plots showing NK cell subcluster-specific gene profiles. (d) Histogram representing the proportion of NK cells subcluster in each group. (e-f) Violin plots showing the levels of Cd69 and Tnf of NK cell subclusters. (g) Heatmap depicting the results of GSEA...

Additional file 4. Figure S4: The effects of MSA-2 and TGF-β on chemokine production in BMDM. (a-f) Unactivated BMDMs were cultured with MSA-2 for one day, and supernatants were collected for chemokine detection.

A stereo interplanting system with foxtail millet beneath chestnut trees is an effective planting method to raise the utilization of land in chestnut orchards, increase yields and improve quality of chestnut nuts. Consequently, exploration of genes involved in shade tolerance response in foxtail millet and breeding shade-tolerant varieties have become urgent issues. In this study, RNA-seq of leaf samples from two shade-tolerant varieties and three shade-intolerant varieties of foxtail millet at the booting stage was...

Chitosan (CS) is commonly used as an adsorbent for wastewater treatment because of its low cost, strong adsorption properties, and high availability of raw materials required for its production. However, CS exhibits limited adaptability to pH, poor mechanical properties, and high swelling in aqueous media; these limitations restrict its widespread use. To address these issues, herein, zeolitic imidazolate framework-67 (ZIF-67) is loaded onto crosslinked CS microspheres (CSM) to prepare CSM@ZIF-67, a composite adsorbent. Next, the...

Supplemental Material for Dynamic response control of centrifugal pendulum vibration absorber with motion-limiting device during startup in gravity field by Yi Zhang, Guangqiang Wu, Yiming Long in Journal of Vibration and Control

Onosma hookeri Clarke. var. longiforum Duthie (OHC-LD), one of the traditional Tibetan medicine, has been found many functions, including removing heat to cool blood, nourishing lung and inhibiting bacteria. In order to study the polysaccharides in OHC-LD water extract, the optimal extraction progress of polysaccharides of the roots of OHC-LD by response surface method designed with three-factor three-level Box-Behnken method and the antioxidant capacity and immune activity of the crude polysaccharide were studied in this...

National holidays are associated with high mortality in some diseases, but little is known about patients undergoing peritoneal dialysis (PD). The research aimed to investigate the impact of national holidays on the health outcomes of PD patients. Over ten years, all episodes of unplanned hospitalization, death, and peritonitis in PD patients were collected in our center. Seven national holidays in China were chosen, and non-holiday days were selected as the control period. The effect of...

To investigate the clinical features of endogenous Klebsiella pneumoniae endophthalmitis (EKPE) and the molecular characteristics of pathogenic K. pneumoniae. A retrospective study was conducted between January 2014 and January 2021. Clinical data were extracted. K. pneumoniae isolates cultured from EKPE patients’ specimens were characterized by antimicrobial susceptibility testing, multilocus sequence typing, capsular serotyping, and virulence gene profiles. Virulence phenotypes were confirmed by mouse lethality assay. Thirty-nine patients (47 eyes) were diagnosed with EKPE. Administration of...

Abstract Background Hepatocellular carcinoma (HCC) is one of the deadliest cancers and is mainly developed from chronic liver diseases such as hepatitis-B infection-associated liver cirrhosis (LC). The progression from LC to HCC makes the detection of diagnostic biomarkers to be challenging. Hence, there have been constant efforts to improve on identifying the critical and predictive changes accompanying the disease progression. Methods In this study, we looked to using the mass spectrometry mediated spatial metabolomics technique...

Objective: Polyneuropathy, organomegaly, endocrinopathy, M-protein, skin changes (POEMS) syndrome is a rare monoclonal plasma cell proliferation disorder. At present, there is no unified treatment for POEMS syndrome. Here, we describe one case with POEMS syndrome with obvious neurological symptoms diagnosed in September 2020. We made a meta-­analysis to assess the efficacy of treatment strategies in recent years. Methods: We retrospectively analyzed the diagnosis and treatment of this patient, and searched relevant articles in PubMed, Embase,...

Description The Road Damage Dataset, RDD2022, is released as a part of the Crowdsensing-based Road Damage Detection Challenge (CRDDC'2022), an IEEE BigData Cup. It comprises 47,420 road images from six countries, Japan, India, the Czech Republic, Norway, the United States, and China. The images have been annotated with more than 55,000 instances of road damage. Four types of road damage, namely longitudinal cracks, transverse cracks, alligator cracks, and potholes, are captured in the dataset....

Additional file 2: Table S1.. Number of different SSR types detected in three Amomum species. Table S3. Species information of Ka/Ks. Table S5. ITS2 haplotypes of A. villosum and A. longiligulare from Sanger sequencing. Table S6. ITS2 reads of A. villosum and A. longiligulare from NGS data. Table S7. The primers and conditions for PCR.

No current RNA-targeted interference tools have been reported to simultaneously up and down-regulate different gene expressions. Here we characterized an RNA-targeted genetic regulatory strategy composed of a flap endonuclease 1 (FEN1) and specific mis-hairpin DNA probes (mis-hpDNA), to realize the orthogonal genetic regulation. By targeting mRNA, the strategy hindered the translation and silenced genes in human cells with efficiencies of ~60%. By targeting miRNA, the strategy prevented the combination of miRNA to its specific mRNA...

Additional file 1: Figure S1. The vector map of (A) wt-circ_0003570-luc, (B) mut-circ_0003570-luc; (C) wt-STARD13-luc, and (D) mut-STARD13-luc. Figure S2. Knockdown of circ_0003570 promotes proliferation, migration and invasion of MHCC-97L cells in vitro. (A) MTT assays and (B) colony formation assays showing that downregulation of circ_0003570 promoted the proliferation of MHCC-97L cells, *P < 0.05. (C) Transwell assays showed circ_0003570 knockdown reduced the migration and invasion of MHCC-97L cells. *P < 0.05. (D) Representative images...

Abstract Background To estimate the incidence, risk factors, and impact on mortality and functional outcomes for early coagulopathy after the return of spontaneous circulation (ROSC) in pediatric out-of-hospital cardiac arrest (OHCA) patients. Methods A post hoc analysis of the Therapeutic Hypothermia after Pediatric Cardiac Arrest Out-of-Hospital (THAPCA-OH) trial was conducted. Early coagulopathy was defined as presence of at least one of the following coagulation abnormalities upon admission: international standard ratio (INR), platelets, and age-adjusted activated...

Additional file 2: Figure S2. A Venn diagram shows the overlap of 22 optimal hub genes between GEO differential genes and WGCNA grey gene set.

Abstract Background Malnutrition is common in critically ill patients, but nutrition status in critically ill patients with acute kidney injury (AKI) has been poorly studied. Our study aimed to investigate the relationship between malnutrition risk and the occurrence and prognosis of AKI in elderly patients in the intensive care unit (ICU). Methods Data were extracted from the Beijing Acute Kidney Injury Trial (BAKIT). A total of 1873 elderly patients were included and compared according to...

Additional file 4: Figure S4. TPM1 knockdown exacerbates inflammation in the TREM2−/− mouse retina. A, B Western blot analysis (A) and quantification of TPM1 (B) in the retina of C57BL/6J (WT) and TREM2−/− mice. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test (WT vs. TREM2−/−, **p < 0.01). C, D Western blot analysis (C) and quantification of TREM2 (D) in WT mice following LPS and siCTR or siTPM1-1...