Additional file 15. Figure S15. Cross talk between cancer cells and BMDCs. BMDCs were treated with 200 ng/ml LPS or different doses of MSA-2. One day later, the supernatant was discarded, and fresh medium was added. The next day, the conditioned medium from BMDCs was used for CT26 and EMT-6 culture. A day later, cancer cells were harvested for flow cytometry assay. Abs targeting H-2Kd (742436, BD) and PD-L1 (124312, BioLegend) were used in the...

Additional file 15. Figure S15. Cross talk between cancer cells and BMDCs. BMDCs were treated with 200 ng/ml LPS or different doses of MSA-2. One day later, the supernatant was discarded, and fresh medium was added. The next day, the conditioned medium from BMDCs was used for CT26 and EMT-6 culture. A day later, cancer cells were harvested for flow cytometry assay. Abs targeting H-2Kd (742436, BD) and PD-L1 (124312, BioLegend) were used in the...

Additional file 1. Figure S1: Flow cytometry for daughter CD4+ T cell in one-way mixed lymphocyte reaction (MLR). Stimulating cells were BMDCs derived from BALB/c mice while responding cells were spleen cells from C57BL/6 in the MLR assays. The mixed cells (the ratio of stimulator to responder = 1:2) were cultured for four days. On day 5, the supernatants and mixed cells were collected for CFSE dilution assay.

Additional file 1. Figure S1: Flow cytometry for daughter CD4+ T cell in one-way mixed lymphocyte reaction (MLR). Stimulating cells were BMDCs derived from BALB/c mice while responding cells were spleen cells from C57BL/6 in the MLR assays. The mixed cells (the ratio of stimulator to responder = 1:2) were cultured for four days. On day 5, the supernatants and mixed cells were collected for CFSE dilution assay.

Additional file 5. Figure S5: MSA-2-stimulated IFN-β expression in cancer cells. Three cell lines B16, CT26, and EMT-6 were cultured with MSA-2 for one day, and supernatants were collected for IFN-β detection with ELISA. BMDM and BMDC were used as positive controls.

Additional file 3: Table S2. The CDSs of ZmABCB family.

Here we proposed a polarization-dependent gradient phase modulation strategy and fabricated a local polarization matched metasurface to add/drop polarization multiplexed cylindrical vector beams (CVBs). The two orthogonal linear polarization states in CVB multiplexing will represent as radial- and azimuthal-polarized CVBs, which means that we must introduce independent wave vectors to them for adding/dropping the polarization channels. By designing the rotation angle and geometric sizes of meta-atom, a local polarization matched propagation phase plasmonic metasurface was...

Here we proposed a polarization-dependent gradient phase modulation strategy and fabricated a local polarization matched metasurface to add/drop polarization multiplexed cylindrical vector beams (CVBs). The two orthogonal linear polarization states in CVB multiplexing will represent as radial- and azimuthal-polarized CVBs, which means that we must introduce independent wave vectors to them for adding/dropping the polarization channels. By designing the rotation angle and geometric sizes of meta-atom, a local polarization matched propagation phase plasmonic metasurface was...

The purpose of this work was to analyze and evaluate the functional properties of a new nanoemulsion that contained cordycepin. Droplet size and distribution were measured, and the stability of the nanoemulsion in different storage conditions was evaluated. We also tested skin irritation caused by the nanoemulsion, cordycepin stability in the nanoemulsion, anti-aging effect, and microbial challenge. The nanoemulsion was homogeneous with proper dispersion stability, and cordycepin was stable for eight months within the cosmetic...

Supplemental material, sj-docx-1-wmr-10.1177_0734242X221139171 for Mini-review of sewage sludge parameters related to system modelling by Huimin Chang, Yan Zhao, Ankun Xu, Anders Damgaard and Thomas H Christensen in Waste Management & Research

Background:Patellar dislocation is attracting considerable research interest.Purpose:To assess studies on patellar dislocation using a scientometric method to better understand the current status of research and explore future study directions.Study Design:Scoping review.Methods:The Web of Science Core Collection database was selected to retrieve publications on patellar dislocation. Articles and reviews written in English with patellar dislocation as the main topic were included. Conference abstracts, notes, letters, expert opinions, and animal studies were excluded. A total of 4632...

Supplemental material, sj-xls-1-tct-10.1177_15330338221139981 for BCL6-SPECC1L: A Novel Fusion Gene in Nasopharyngeal Carcinoma by Shuo-Gui Fang, Tian-Liang Xia, Jian-Chang Fu, Tong Li, Qian Zhong and Fei Han in Technology in Cancer Research & Treatment

Supplemental material, sj-docx-3-tct-10.1177_15330338221139981 for BCL6-SPECC1L: A Novel Fusion Gene in Nasopharyngeal Carcinoma by Shuo-Gui Fang, Tian-Liang Xia, Jian-Chang Fu, Tong Li, Qian Zhong and Fei Han in Technology in Cancer Research & Treatment

The structure design, performance analysis, and process optimization of CNT/MOF-derived hierarchical composite play an important role in the development of high-performance microwave absorbing materials. Herein, the preparation, morphology evolution, and electromagnetic wave absorption mechanism of CNT/MOF-derived hierarchical composite were systematically investigated. The regulation mechanism was revealed by studying the changes in the morphological characteristics, electromagnetic properties, and microwave absorbing performance of CNT/MOF-derived hierarchical composite under different process parameters. The results show that the morphological characteristics...

Background: It remains controversial whether the application of chemotherapy has an impact on recurrent nasopharyngeal carcinoma (rNPC) patients with salvage radiotherapy. Here, we aimed to evaluate treatment outcomes of rNPC patients and derive a prognostic model to assess the benefit of chemotherapy in patients with re-radiotherapy. Methods: This study was conducted as a retrospective study. In total, 340 rNPC patients treated with salvage intensity-modulated radiotherapy (IMRT) or radiochemotherapy (RCT) from October 2006 to September 2019...

Supplemental Material for Preparing low-Density microcellular polystyrene foam by in-Situ fibrillated PTFE and supramolecular nucleator TMC-300 in the presence of sc-CO2 by Zhuolun Li, Xiangdong Wang, Yaqiao Wang and Shihong Chen in Journal of Cellular Plastics

This cross-sectional and longitudinal analysis aimed to demonstrate the disparities in positive results and dissemination patterns of randomized controlled trials (RCTs) in global immuno-oncology (IO). Phase II–IV RCTs with results reported by article publications registered on ClinicalTrials.gov in 2007–2018 studying immune checkpoint inhibitors (ICIs), adoptive cell transfer, cancer vaccines, and immune modulators were included. Twenty-eight percent of trials were positive (72 of 258), most of which were pharma-sponsored and focused on ICI and multiple IO...

This cross-sectional and longitudinal analysis aimed to demonstrate the disparities in positive results and dissemination patterns of randomized controlled trials (RCTs) in global immuno-oncology (IO). Phase II–IV RCTs with results reported by article publications registered on ClinicalTrials.gov in 2007–2018 studying immune checkpoint inhibitors (ICIs), adoptive cell transfer, cancer vaccines, and immune modulators were included. Twenty-eight percent of trials were positive (72 of 258), most of which were pharma-sponsored and focused on ICI and multiple IO...

Boarding and alighting process is the most complicated behaviour at urban railway stations, and significantly affects the service level and evacuation capacities of stations. This paper quantitatively discusses the individual and group behavioural characteristics of both boarding and alighting passengers. The time headways are extracted from surveillance videos. Based on the data analysis results, an improved cellular automata model is proposed to describe the microscopic dynamics of the boarding and alighting process. In this model,...

Circular RNAs (circRNAs) correlate with cancer cell phenotypes. Particularly, circRNAs mediate the cancer process as microRNAs (miRNAs) sponges. This study was to ascertain the roles of hsa_circ_0009910 in phenotypic aspects of ovarian cancer cells. Mantel-Cox test was performed to analyze the correlation between hsa_circ_0009910 and survival outcomes of ovarian cancer. Minigene reporter was constructed and small interfering-RNA was designed for constructing hsa_circ_0009910-dysregulated and miR-145-upregulated cells identified by qRT-PCR. Proliferative and motile activities were monitored by...

As both a vibrant industry and necessity for people’s lives, housing has attracted much attention in recent years. This article examines the efficacy of both loosening and tightening policies on mortgage lending in different priced housing markets in Shanghai, China, from 2014 to 2018 using daily average sale prices across 1,824 neighborhoods, with price-classified cluster analysis and policy-informed hedonic regressions. The results show that (1) the effects of mortgage lending policy interventions varied significantly and...

Background:Bony remodeling of displaced clavicle fractures in adolescents remains poorly understood.Purpose:To evaluate and quantify clavicle remodeling in a large population of adolescents with completely displaced fractures, which were treated nonoperatively, to better understand the factors that may influence this process.Study Design:Case series; Level of evidence, 4.Methods:Patients were identified from the databases of a multicenter study group investigating the functional outcomes of adolescent clavicle fractures. Patients between the ages of 10 and 19 years with completely...

Supplemental material, sj-pdf-1-ajs-10.1177_03635465231152884 for Bony Remodeling of Adolescent Displaced Clavicle Fractures: A FACTS Study by Andrew T. Pennock, Tracey P. Bastrom, Kelly E. Boutelle, Alyssa N. Carroll, Eric W. Edmonds, Jeffrey J. Nepple, Samuel G. Polinsky, David D. Spence, Benton E. Heyworth, Crystal Perkins, Samuel C. Willimon, Donald S. Bae, Michael T. Busch, Henry B. Ellis, Katelyn Hergott, Mininder S. Kocher, Ying Li, Nirav K. Pandya, Coleen S. Sabatini and Philip L. Wilson in The...

Abstract In December 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began to break out in the Hubei Province of China. At present, the epidemic situation in the world continues and the number of confirmed cases is increasing every day. A recent review showed that children under the age of ten years make up about 1% of the infected population, which cannot be ignored. Studies have shown that after SARS-CoV-2 infection children can show...

Passive-state-preparation (PSP) continuous-variable quantum key distribution (CVQKD) protocol explores the intrinsic field fluctuations of a thermal source. Compared with traditional Gaussian-modulated coherent-state (GMCS) CVQKD, it does not need active modulations and has promising applications in chip integration and portable free-space quantum key distribution. In this Letter, we propose and experimentally realize a PSP CVQKD scheme with transmitted local oscillator (LO) through fluctuating transmittance free-space channel using off-the-shelf amplified spontaneous emission source for the first time....