823 Works

Additional file 2 of PRR34-AS1 promotes exosome secretion of VEGF and TGF-β via recruiting DDX3X to stabilize Rab27a mRNA in hepatocellular carcinoma

Zhilei Zhang, Ye Zhou, Yuming Jia, Chao Wang, Meng Zhang & Zhuo Xu
Additional file 2: Figure S2. DDX3X positively regulates Rab27a expression in HCC cells. A The cellular distribution of PRR34-AS1 in HCC cells was confirmed by subcellular fractionation assay. N = 3. B, C The mRNA and protein levels of DDX3X and Rab27a were analyzed in HCC cells transfected with sh/DDX3X#a/b. N = 3. **P < 0.01. Data were analyzed via DGP PCR quantification.

Additional file 2 of PRR34-AS1 promotes exosome secretion of VEGF and TGF-β via recruiting DDX3X to stabilize Rab27a mRNA in hepatocellular carcinoma

Zhilei Zhang, Ye Zhou, Yuming Jia, Chao Wang, Meng Zhang & Zhuo Xu
Additional file 2: Figure S2. DDX3X positively regulates Rab27a expression in HCC cells. A The cellular distribution of PRR34-AS1 in HCC cells was confirmed by subcellular fractionation assay. N = 3. B, C The mRNA and protein levels of DDX3X and Rab27a were analyzed in HCC cells transfected with sh/DDX3X#a/b. N = 3. **P < 0.01. Data were analyzed via DGP PCR quantification.

Additional file 3 of PRR34-AS1 promotes exosome secretion of VEGF and TGF-β via recruiting DDX3X to stabilize Rab27a mRNA in hepatocellular carcinoma

Zhilei Zhang, Ye Zhou, Yuming Jia, Chao Wang, Meng Zhang & Zhuo Xu
Additional file 3: Figure S3. Transfection efficiency of Rab27a in HCC cells. A Rab27a levels in HCC cells was detected by RT-qPCR and western bot analyses after Rab27a silencing. N = 3. **P < 0.01.

Seasonal distribution of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in Tibetan sheep in Qinghai, China

Fan Yang, Li Ma, Jing-min Gou, Hui-zhong Yao, Mei Ren, Bing-ke Yang & Qing Lin
Abstract Background Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi can cause important intestinal diseases in ruminants. However, data on the distribution of these three protozoan pathogens in Tibetan sheep are limited. Methods We collected 761 fecal samples from Tibetan sheep across four seasons in Qinghai Province, China, and screened the samples for Cryptosporidium spp., G. duodenalis and E. bieneusi using PCR-based sequence analysis of the genes encoding 18S ribosomal RNA, triosephosphate isomerase and the internal...

Additional file 8 of Biallelic mutations of TTC12 and TTC21B were identified in Chinese patients with multisystem ciliopathy syndromes

Weicheng Chen, Feifei Wang, Weijia Zeng, Xinyan Zhang, Libing Shen, Yuan Zhang & Xiangyu Zhou
Additional file 8: Table S4. Primers used for real-time PCR and cDNA amplification of TTC12

Supplementary material from \"New macronarian from the Middle Jurassic of Chongqing, China: phylogenetic and biogeographic implications for neosauropod dinosaur evolution\"

Hui Dai, Chao Tan, Qingyu Ma, Ning Li, Haidong Yu, Can Xiong, Zhaoying Wei, Ping Wang, Jian Yi, Guangbiao Wei, Hailu You & Xinxin Ren
Macronaria is a clade of gigantic body-sized sauropod dinosaurs widely distributed from the Late Jurassic to the Late Cretaceous globally. However, its origin, early diversification, and dispersal are still controversial. Here, we report a new macronarian Yuzhoulong qurenensis gen. et sp. nov. excavated from the Middle Jurassic (Bathonian) Lower Shaximiao Formation. Yuzhoulong qurenensis bears a unique combination of features, such as two accessory fossae that exist on the posterior surface of dorsal diapophyses of anterior...

Tim-3 downregulation by Toxoplasma gondii infection contributes to decidual dendritic cell dysfunction

Hongbing Xie, Zhidan Li, Guangmei Zheng, Chunyan Yang, Xianbing Liu, Xiaoyan Xu, Yushan Ren, Chao Wang & Xuemei Hu
Abstract Background Women in early pregnancy infected by Toxoplasma gondii may have severe adverse pregnancy outcomes, such as spontaneous abortion and fetal malformation. The inhibitory molecule T cell immunoglobulin and mucin domain 3 (Tim-3) is highly expressed on decidual dendritic cells (dDCs) and plays an important role in maintaining immune tolerance. However, whether T. gondii infection can cause dDC dysfunction by influencing the expression of Tim-3 and further participate in adverse pregnancy outcomes is still...

DNA methylation alternation in Stanford- A acute aortic dissection

Yufei Chen, Xu Xu, Zhaoran Chen, Bi Huang, Xiaojian Wang & Xiaohan Fan
Abstract Background Acute aortic dissection (AAD) is a life-threatening cardiovascular disease. Recent studies have shown that DNA methylation may be associated with the pathological mechanism of AAD, but the panorama of DNA methylation needs to be explored. Methods DNA methylation patterns were screened using Infinium Human Methylation 450 K BeadChip in the aortic tissues from 4 patients with Stanford-A AAD and 4 controls. Gene enrichment was analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG)...

Directional porous polyimide/polyethylene glycol composite aerogel with enhanced CO2 uptake performance

Yihao Nie, Xibin Yi, Xinfu Zhao, Shimo Yu, Jing Zhang, Xiaochan Liu, Sijia Liu, Zhipeng Yuan & Minna Zhang
The cost of CO2 separation and energy consumption can be decreased through the use of CO2 adsorption. Due to the electron-rich heteroatoms in its network, polyimide (PI) has a remarkable affinity for CO2. Polyethylene glycol (PEG) can increase the layer spacing of polymers, so as to change the mass transfer of CO2 in it. Furthermore, the ether bond (-O-) in PEG has good affinity for CO2. In this study, PEG-1000 was introduced into PI aerogel...

Additional file 1 of Seasonal distribution of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in Tibetan sheep in Qinghai, China

Fan Yang, Li Ma, Jing-min Gou, Hui-zhong Yao, Mei Ren, Bing-ke Yang & Qing Lin
Additional file 1. Dataset S1. The nucleotide sequences detected on the triosephosphate isomerase (TPI) genetic locus of Giardia duodenalis.

Additional file 1 of Comparative chloroplast genome analyses of Amomum: insights into evolutionary history and species identification

Lu Gong, Xiaoxia Ding, Wan Guan, Danchun Zhang, Jing Zhang, Junqi Bai, Wen Xu, Juan Huang, Xiaohui Qiu, Xiasheng Zheng, Danyan Zhang, Shijie Li, Zhihai Huang & He Su
Additional file 1: Table S2.. Genbank accession numbers of DNA barcodes. Table S4. Results for one-vs-one calculation of Ka/Ks generated by KaKs_Calculator.

Additional file 3 of Comparative chloroplast genome analyses of Amomum: insights into evolutionary history and species identification

Lu Gong, Xiaoxia Ding, Wan Guan, Danchun Zhang, Jing Zhang, Junqi Bai, Wen Xu, Juan Huang, Xiaohui Qiu, Xiasheng Zheng, Danyan Zhang, Shijie Li, Zhihai Huang & He Su
Additional file 3: Fig. S1. Analysis of simple sequence repeats (SSRs) in the cp genomes of three Amomum species. Fig. S2. Frequency of identified SSR motifs in different repeat class types. Fig. S3. Repeat sequences of three Amomum species. F, P, R, and C indicate the repeat types F (forward), P (palindrome), R (reverse), and C (complement), respectively. Fig. S4. ML tree based on ccsA sequences of Amomum species. This Bootstrap consensus tree was constructed...

Additional file 1 of Comprehensive virome analysis of the viral spectrum in paediatric patients diagnosed with Mycoplasma pneumoniae pneumonia

Qiong Guo, Lili Li, Chao Wang, Yiman Huang, Fenlian Ma, Shanshan Cong, Jingjing Tan, Lihong Yao, Aijun Chen & Lishu Zheng
Additional file 1. The tables of the filtering parameters setting using Prinseq-lite software, primers and probes used to detect co-infection respiratory viruses, type analysis of common virus-related sequence viral in MP positive and MP negative (TTV has been removed). Table S1: The filtering parameters setting using Prinseq-lite software. Table S2: Primers and probes used to detect co-infection respiratory viruses. Table S3: Type analysis of common virus-related sequence viral in MP positive and MP negative (TTV...

Comprehensive virome analysis of the viral spectrum in paediatric patients diagnosed with Mycoplasma pneumoniae pneumonia

Qiong Guo, Lili Li, Chao Wang, Yiman Huang, Fenlian Ma, Shanshan Cong, Jingjing Tan, Lihong Yao, Aijun Chen & Lishu Zheng
Abstract Background Among hospitalized children suffering from community-acquired pneumonia, Mycoplasma pneumoniae (MP) is one of the most common pathogens. MP often exists as a co-infection with bacteria or viruses, which can exacerbate the clinical symptoms. We investigated the pathogen spectrum in MP-positive and MP-negative samples from hospitalized children with respiratory tract infections in Beijing, China. Method This study included 1038 samples of nasopharyngeal aspirates obtained between April, 2017 and March, 2018 from hospitalized children under...

The palynology of the Permian succession in the CSDP-2 Well, South Yellow Sea, China

Xing-Kai Ji, Xing-Wei Guo, Ning Yang, Jiří Bek, Ting Nie, Hui-Nan Lu & Hong-He Xu
The fully cored CSDP-2 Well, drilled in the central uplift region of the South Yellow Sea, China, provides indispensable and very significant research opportunities to understand the evolution of life, paleoclimate, paleogeography, and tectonics during the Permian in the South Yellow Sea area of the Yangtze paleoblock. We here analyze 118 samples from 893.7 to 1798.8 m depth of the CSDP-2 borehole core and recognize three Permian palynological assemblages, Laevigatosporites minimus – Florinites florini (MF)...

Additional file 1 of Whole-transcriptome profiles of Chrysanthemum seticuspe improve genome annotation and shed new light on mRNA–miRNA–lncRNA networks in ray florets and disc florets

Daojin Sun, Jing Zhang, Jun He, Zhiqiang Geng, Song Li, Jiali Zhang, Peiling Li, Lingling Zhang, Zhenxing Wang, Likai Wang, Fadi Chen & Aiping Song
Additional file 1.

Mining and characterization of the PKS–NRPS hybrid for epicoccamide A: a mannosylated tetramate derivative from Epicoccum sp. CPCC 400996

Tao Zhang, Guowei Cai, Xiaoting Rong, Jingwen Xu, Bingya Jiang, Hao Wang, Xinxin Li, Lu Wang, Ran Zhang, Wenni He & Liyan Yu
Abstract Background Genomic analysis indicated that the genomes of ascomycetes might carry dozens of biosynthetic gene clusters (BGCs), yet many clusters have remained enigmatic. The ascomycete genus Epicoccum, belonging to the family Didymellaceae, is ubiquitous that colonizes different types of substrates and is associated with phyllosphere or decaying vegetation. Species of this genus are prolific producers of bioactive substances. The epicoccamides, as biosynthetically distinct mannosylated tetramate, were first isolated in 2003 from Epicoccum sp. In...

N6-methyladenosine-mediated SH3BP5-AS1 upregulation promotes GEM chemoresistance in pancreatic cancer by activating the Wnt signaling pathway

Chengjie Lin, Yan Wang, Yihong Dong, Shihui Lai, Liang Wang, Shangeng Weng & Xiang Zhang
Abstract Background Pancreatic cancer (PC) is highly malignant. Chemotherapy is the main treatment strategy, especially for patients with advanced PC. However, chemoresistance has always been a frequently encountered bottleneck. Hence, there is an urgent need to enhance the sensitivity of PC to gemcitabine (GEM). Results We demonstrated that SH3BP5-AS1 was significantly upregulated in GEM-resistant PC and predicted a poorer prognosis. SH3BP5-AS1 stability was regulated by ALKBH5/IGF2BP1-mediated m6A modification. Loss of SH3BP5-AS1 reduced PC cell migration...

N6-methyladenosine-mediated SH3BP5-AS1 upregulation promotes GEM chemoresistance in pancreatic cancer by activating the Wnt signaling pathway

Chengjie Lin, Yan Wang, Yihong Dong, Shihui Lai, Liang Wang, Shangeng Weng & Xiang Zhang
Abstract Background Pancreatic cancer (PC) is highly malignant. Chemotherapy is the main treatment strategy, especially for patients with advanced PC. However, chemoresistance has always been a frequently encountered bottleneck. Hence, there is an urgent need to enhance the sensitivity of PC to gemcitabine (GEM). Results We demonstrated that SH3BP5-AS1 was significantly upregulated in GEM-resistant PC and predicted a poorer prognosis. SH3BP5-AS1 stability was regulated by ALKBH5/IGF2BP1-mediated m6A modification. Loss of SH3BP5-AS1 reduced PC cell migration...

Screening of ulcerative colitis biomarkers and potential pathways based on weighted gene co-expression network, machine learning and ceRNA hypothesis

Ying Li, Mengyao Tang, Feng Jun Zhang, Yihan Huang, Jing Zhang, Junqi Li, Yunpeng Wang, Jinguang Yang & Shu Zhu
Abstract Background Ulcerative colitis (UC) refers to an intractable intestinal inflammatory disease. Its increasing incidence rate imposes a huge burden on patients and society. The UC etiology has not been determined, so screening potential biomarkers is critical to preventing disease progression and selecting optimal therapeutic strategies more effectively. Methods The microarray datasets of intestinal mucosal biopsy of UC patients were selected from the GEO database, and integrated with R language to screen differentially expressed genes...

Supplementary document for Silicon Bowtie Structure Based Adjustable Non-rigid All Nonmetal Metamaterials Terahertz Filter - 6122688.pdf

Danni Hao, Yi Zhang, Dongxun Yang, Rui Li, Zhao Dan, Zhenzhen Zhang, Shuai Wang, Wei Jin, Hao Tian & Jiaying Duan
supporting information

Additional file 2 of Exploring the pharmacological mechanism of Shengjing capsule on male infertility by a network pharmacology approach

Ming Wang, Qi Wang, Hui Jiang, Yongqiang Du & Xiansheng Zhang
Additional file 2:

Additional file 3 of Exploring the pharmacological mechanism of Shengjing capsule on male infertility by a network pharmacology approach

Ming Wang, Qi Wang, Hui Jiang, Yongqiang Du & Xiansheng Zhang
Additional file 3:

Additional file 4 of Exploring the pharmacological mechanism of Shengjing capsule on male infertility by a network pharmacology approach

Ming Wang, Qi Wang, Hui Jiang, Yongqiang Du & Xiansheng Zhang
Additional file 4:

Exploring the pharmacological mechanism of Shengjing capsule on male infertility by a network pharmacology approach

Ming Wang, Qi Wang, Hui Jiang, Yongqiang Du & Xiansheng Zhang
Abstract Background Shengjing capsule (SJC) is a traditional Chinese medicine (TCM) and has gained widespread clinical application for the treatment of male infertility (MI). However, the pharmacological mechanism of SJC against MI remains vague to date. Method The active ingredients of SJC and their targets were identified from the database, and MI-related genes were retrieved from several databases. Protein–protein interaction (PPI) data were obtained to construct the PPI networks. The candidate targets of SJC against...

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