607 Works

Angiopoietin-like protein 8, molecular cloning and regulating lipid accumulation in goat intramuscular preadipocytes

Wei Huang, Yaqiu Lin, Hua Xiang, Jiangjiang Zhu & Yong Wang
This study aimed to clone the full-length open reading frame (ORF) of goat ANGPTL8 gene sequence, reveal its molecular and expression characteristics, and explore its effect on the differentiation of goat intramuscular preadipocytes. The full-length ORF sequence of goat ANGPTL8 gene was cloned by RT-PCR technology, and bioinformatics analysis was performed by related biological software. RT-qPCR was used to detect the expression of ANGPTL8 mRNA in goat tissues. Further use of RNA interference to study...

Long non-coding RNA RP11-283G6.5 confines breast cancer development through modulating miR-188-3p/TMED3/Wnt/β-catenin signaling

Jing Pei, Shengquan Zhang, Xiaowei Yang, Chunguang Han, Yubo Pan, Jun Li, Zhaorui Wang, Chenyu Sun & Jing Zhang
The contributions of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) to breast cancer are critical areas of investigation. In this study, we identified a novel lncRNA RP11-283G6.5 which was lowly expressed in breast cancer and whose low expression was correlated with poor overall survival and disease-free survival of breast cancer patients. Functional experiments revealed that ectopic expression of RP11-283G6.5 confined breast cancer cellular growth, migration, and invasion, and promoted cellular apoptosis. Conversely, RP11-283G6.5 silencing facilitated...

Circular RNA circ_0001162 promotes cell proliferation and invasion of glioma via the miR-936/ERBB4 axis

Dexiang Zhou, Xiaofeng Lin, Peng Wang, Yong Yang, Jiantao Zheng & Dong Zhou
The biological modulatory roles of many circular RNAs (circRNAs) have been validated in glioma. The current study was designed to research the functional mechanism of circ_0001162 in glioma progression. The quantitative real-time polymerase chain reaction (qRT-PCR) was used for assaying the levels of circ_0001162 and microRNA-936 (miR-936). Cell proliferation and colony formation abilities were evaluated via 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) and colony formation assay, respectively. Transwell assay was applied to assess cell migration...

Circular RNA circ_0001162 promotes cell proliferation and invasion of glioma via the miR-936/ERBB4 axis

Dexiang Zhou, Xiaofeng Lin, Peng Wang, Yong Yang, Jiantao Zheng & Dong Zhou
The biological modulatory roles of many circular RNAs (circRNAs) have been validated in glioma. The current study was designed to research the functional mechanism of circ_0001162 in glioma progression. The quantitative real-time polymerase chain reaction (qRT-PCR) was used for assaying the levels of circ_0001162 and microRNA-936 (miR-936). Cell proliferation and colony formation abilities were evaluated via 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) and colony formation assay, respectively. Transwell assay was applied to assess cell migration...

Transcriptional dynamics of transposable elements when converting fibroblast cells of Macaca mulatta to neuroepithelial stem cells

Dahai Liu, Li Liu, Kui Duan, Junqiang Guo, Shipeng Li, Zhigang Zhao, Xiaotuo Zhang, Nan Zhou & Yun Zheng
Abstract Background Transposable elements (TE) account for more than 50% of human genome. It has been reported that some types of TEs are dynamically regulated in the reprogramming of human cell lines. However, it is largely unknown whether some TEs in Macaca mulatta are also regulated during the reprogramming of cell lines of monkey. Results Here, we systematically examined the transcriptional activities of TEs during the conversion of Macaca mulatta fibroblast cells to neuroepithelial stem...

MicroRNA-146b-3p regulates the dysfunction of vascular smooth muscle cells via repressing phosphoinositide-3 kinase catalytic subunit gamma

Xijing Zhuang, Feng Gao, Lei Shi, Wei Liu, Wenjun Wang, Xuezhi He & Yang Gao
MicroRNAs are crucial regulators in the phenotype switch of vascular smooth muscle cells (VSMCs). Nonetheless, the role of miR-146b-3p in VSMCs remains unclear. In the present study, platelet-derived growth factor-BB (PDGF-BB) at different concentrations was employed to stimulate VSMCs for different times, to establish the model of VSMC dysfunction. The relative expression of miR-146b-3p was quantified by quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation of VSMCs was measured by BrdU assay. Flow cytometry analysis...

Fat mass and obesity-associated protein (FTO) mediates signal transducer and activator of transcription 3 (STAT3)-drived resistance of breast cancer to doxorubicin

Yan Wang, Zhiqiang Cheng, Jing Xu, Meina Lai, Liming Liu, Min Zuo & Lin Dang
Excessive activation of signal transducer and activator of transcription 3 (STAT3) is implicated in breast cancer (BC) chemoresistance, but its underlying mechanism is not fully understood. There are STAT3 binding sites in fat mass and obesity-associated protein (FTO) promoter region, thus STAT3 may regulate the transcription of FTO. This study aimed to investigate the correlation between FTO and STAT3 in BC chemoresistance. Herein, FTO and STAT3 were highly expressed in doxorubicin-resistant BC (BC-DoxR) cells. CHIP...

The safety and tolerability of alkaloids from Alstonia scholaris leaves in healthy Chinese volunteers: a single-centre, randomized, double-blind, placebo-controlled phase I clinical trial

Zhong-Ping Gou, Yun-Li Zhao, Lin-Ling Zou, Ying Wang, Shi-Qing Shu, Xiao-Hong Zhu, Li Zheng, Qi Shen, Zhu Luo, Jia Miao, Yong-Sheng Wang, Xiao-Dong Luo & Ping Feng
Capsule of alkaloids from the leaf of Alstonia scholaris (L.) R.Br. (Apocynaceae) (CALAS) is a new investigational botanical drug (No. 2011L01436) for bronchitis, post-infectious cough and asthma. To observe the clinical safety and tolerability of CALAS. Subjects were assigned to eight cohorts, and each received randomly CALAS or placebo in one of single ascending dose (SAD) of 8, 40, 120, 240, 360, 480, or in one of multiple ascending dose (MAD) of 40 or 120...

Comprehensive Analysis of Monocarboxylate Transporter 4 (MCT4) expression in breast cancer prognosis and immune infiltration via integrated bioinformatics analysis

Chen Yuan, Jie Zhang, Jianjuan Lou, Siqi Wang, Yanni Jiang, Feiyun Wu & Shouju Wang
Lactate blunts the anticancer immune response in breast cancer (BC). However, little is known about the exact effect of lactate transporters such as monocarboxylate transporter 4 (MCT4) on immunotherapy. In this study, we investigated the expression status and prognostic value of MCT4 in BC through large-scale transcriptome data. Our results showed that MCT4 was overexpressed in BC, particularly in the basal-like molecular subtype. Overexpression of MCT4 was significantly correlated with high BC lesion grade and...

The effects of ground cover management on fruit yield and quality: a meta-analysis

Linfa Fang, Xiaojun Shi, Yong Zhang, Yehua Yang, Xueliang Zhang, Xiaozhong Wang & Yuting Zhang
An important management strategy for the development of sustainable agriculture in orchards is the management of ground cover. However, there is a knowledge gap regarding the effects of ground cover on fruit yield and quality. We performed a meta-analysis of 580 observations collected from 116 peer-reviewed field studies, to assess the effects of ground cover on fruit yield and quality indicators. Overall, ground cover significantly increased fruit yield, soluble solid content, sugar content, and vitamin...

Heterologous expression and enzymatic identification of two novel soluble pyridine nucleotide transhydrogenases from Acidobacteria bacterium KBS 146 and Nocardia jiangxiensis

Zhengyu Cao, Rui Meng, Peng Wang & Guoping Zhu
Soluble pyridine nucleotide transhydrogenase (STH), catalyzes a reversible hydrogen transfer between NADH and NADPH, is widely utilized in cofactor engineering. In the present study, we expanded the distribution range of STH to more unreported bacteria. Meanwhile, STH was mainly found in α, γ, δ-proteobacteria, acidobacteria, actinobacteria and planctomycetes. The enzymological properties of two novel STHs from Acidobacteria bacterium KBS 146 (AbSTH) and Nocardia jiangxiensis (NjSTH) were characterized. The optimum temperature and pH of AbSTH and...

Additional file 8 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 8: Table S5. Sequence of primers used in this study.

Effects of percutaneous balloon mitral valvuloplasty in patients with mitral stenosis and atrial fibrillation: a systematic review and meta-analysis

Bojiang Liu, Yunyun Wang, Yingwu Liu, Peng Wu & Tong Li
The study aimed to systematically review the existing literature and assess the effects of percutaneous balloon mitral valvuloplasty (PBMV) in patients with mitral stenosis and atrial fibrillation (AF) as opposed to sinus rhythm (SR). Eligible studies were identified from six electronic databases before June 2021. The primary outcome was mitral valve area (MVA), and secondary outcomes were hemodynamic measurements, in-hospital complications, and long-term outcomes. Relative risks (RRs) or weighted mean differences (WMDs) with 95% confidence...

Additional file 2 of Using a WeChat mini-program-based lactation consultant intervention to increase the consumption of mother’s own milk by preterm infants in the neonatal intensive care unit: a study protocol for a cluster randomized controlled trial

Jie Huo, Xinping Wu, Chuanli Gu, Zhangbin Yu, Jun Zhang, Xiaohui Chen, Jingai Zhu, Feng Liu, Beibei Liu, Qianqian Li & Shuping Han
Additional file 2.

Heterogeneous performances and consequences of China’s industrial environmental governance: clean production vs. end-of-pipe treatment

Shuai Shao, Chang Wang, Yue Guo, Bai-Chen Xie, Zhihua Tian & Shiyi Chen
Using a relational two-stage network data envelopment analysis (DEA) model considering undesirable outputs, this paper evaluates both the process and system performances of industrial environmental governance in China’s 30 provincial-level regions during 1998–2015. Furthermore, this is the first study to apply the spatial econometric models with a geographical distance matrix to investigate the local and spatial spillover effects of environmental regulations on heterogeneous environmental governance performance (i.e. clean production and end-of-pipe treatment performances). The results...

Three periods of gold mineralization in the Liaodong Peninsula, North China Craton

Peng Zhang, Linlin Kou & Yang Zhao
The Liaodong gold province includes voluminous gold deposits, which occur in an Archean gneiss complex, Paleoproterozoic metamorphic rocks, and Mesozoic granite. However, the ore-forming age, the genesis, and the geodynamic setting of the gold deposits are not well constrained. In this study, we have summarized previous geochronology data, H-O-S-Pb isotopes, and He-Ar isotopic compositions of the gold deposits. Based on geochronology data, the gold mineralization was divided into Late Triassic, Early Jurassic, and Early Cretaceous....

Aucubin slows the development of osteoporosis by inhibiting osteoclast differentiation via the nuclear factor erythroid 2-related factor 2-mediated antioxidation pathway

Yongfeng Zhang, Xin Liu, Yangyang Li, Minkai Song, Yutong Li, Anhui Yang, Yaqin Zhang, Di Wang & Min Hu
Osteoporosis (OP) is a metabolic disease. We have previously demonstrated that aucubin (AU) has anti-OP effects that are due to its promotion of the formation of osteoblasts. To investigate the mechanisms of anti-OP effects of AU. C57BL/6 mice were randomly divided into control group, 30 mg/kg Dex-induced OP group (OP model group, 15 μg/kg oestradiol-treated positive control group, 5 or 45 mg/kg AU-treated group), and 45 mg/kg AU-alone-treated group. The administration lasted for 7 weeks....

Using a WeChat mini-program-based lactation consultant intervention to increase the consumption of mother’s own milk by preterm infants in the neonatal intensive care unit: a study protocol for a cluster randomized controlled trial

Jie Huo, Xinping Wu, Chuanli Gu, Zhangbin Yu, Jun Zhang, Xiaohui Chen, Jingai Zhu, Feng Liu, Beibei Liu, Qianqian Li & Shuping Han
Abstract Background The benefits of mother’s own milk (MOM) for preterm infants have been widely recognized. Many studies have shown that the rate of breastfeeding of premature infants remains very low. Although many studies use measures to promote breastfeeding, few high-quality cluster randomized controlled studies have evaluated the effectiveness of these measures. WeChat is an instant messaging software for smart terminals, and WeChat mini-programs have been widely used to promote health and self-management in China....

Spatiotemporal specificity of correlated DNA methylation and gene expression pairs across different human tissues and stages of brain development

Kangli Wang, Rujia Dai, Yan Xia, Jianghua Tian, Chuan Jiao, Tatiana Mikhailova, Chunling Zhang, Chao Chen & Chunyu Liu
DNA methylation (DNAm) that occurs on promoter regions is primarily considered to repress gene expression. Previous studies indicated that DNAm could also show positive correlations with gene expression. Both DNAm and gene expression profiles are known to be tissue- and development-specific. This study aims to investigate how DNAm and gene expression are coordinated across different human tissues and developmental stages, as well as the biological significance of such correlations. By analyzing 2,239 samples with both...

Up-regulation of microRNA miR-101-3p enhances sensitivity to cisplatin via regulation of small interfering RNA (siRNA) Anti-human AGT4D and autophagy in non-small-cell lung carcinoma (NSCLC)

Dong Cui, Yu Feng & Rulin Qian
The emergence of drug resistance hinders the treatment of malignant tumors, and autophagy plays an important role in tumor chemotherapy resistance. However, its mechanism in non-small cell lung cancer (NSCLC) has not been well-researched. We aim to investigate the role of miR-101-3p in cisplatin-resistant via regulation of autophagy-related protein 4D (ATG4D) and autophagy. Cell viability, apoptosis, fluorescence intensity of GFP-LC3 and RFP-GFP-LC3 were determined using Cell Counting Kit-8 (CCK-8) assay, flow cytometry, and Laser scanning...

Ages and tectonic attributes of Late Palaeozoic stratigraphic units in the Tarbagatay, West Junggar (NW China): revisited

Yan Xu, Ang Li, Wen Liao & Bao-Fu Han
As the youngest single age of detrital zircons is used as the maximum depositional age, the ages and tectonic attributes of the Late Palaeozoic stratigraphic units in the Tarbagatay area, West Junggar, NW China, become very confusing. An improved statistical approach indicates that 5, 6 and 7 concordant zircons are required for the maximum depositional age at 95% confidence level when the quantity of total concordant zircon in a sample does not exceed 50, 140...

sRNA23, a novel small RNA, regulates to the pathogenesis of Streptococcus suis serotype 2

Quanming Xu, Hong Chen, Wen Sun, Yongyi Zhang, Dewen Zhu, Kul Raj Rai, Ji-Long Chen & Ye Chen
Streptococcus suis serotype 2 (S. suis 2) is an important ubiquitous zoonotic pathogen. To date, regulatory factors and their implication in S. suis pathogenesis are not fully understood. Small non-coding RNAs (sRNAs) have been proven to function as important regulatory factors in bacterial pathogenesis and stress adaptation. Here, we identified a differentially downregulated S. suis 05ZYH33 sRNA after iron starvation by RNA-seq, which we named sRNA23. The presence of sRNA23 was further confirmed by RACE...

Cessation of collisional tectonism and rapid crustal uplift recorded by 430–420 Ma igneous rocks in the South Kunlun belt, northwest China

Jing Chen, Bing-Zhang Wang, Fu-Cheng Yu, Jie Han & Wu-Fu Li
The Wanbaogou granitic plutons are exposed along with coeval volcanic rocks, and provide an opportunity to investigate Silurian tectonism in the South Kunlun belt, northwest China. This study presents new whole-rock geochemical, and zircon U–Pb geochronological and Lu–Hf isotope data for Silurian intrusive rocks of the Wanbaogou area. We analysed four samples: a porphyritic monzogranite and dioritic dike from the Wanbaogou pluton and a granodiorite and monzogranite from the Wudaogou pluton. Zircon U–Pb dating yielded...

Toward Optimal Fingerprinting in Detection and Attribution of Changes in Climate Extremes

Zhuo Wang, Yujing Jiang, Hui Wan, Jun Yan & Xuebin Zhang
Abstract–Detection and attribution of climate change plays a central role in establishing the causal relationship between the observed changes in the climate and their possible causes. Optimal fingerprinting has been widely used as a standard method for detection and attribution analysis for mean climate conditions, but there has been no satisfactory analog for climate extremes. Here, we turn an intuitive concept, which incorporates the expected climate responses to external forcings into the location parameters of...

An infectious clone of enterovirus 71(EV71) that is capable of infecting neonatal immune competent mice without adaptive mutations

Huiying Zhang, Zhigang Song, Jingyi Zou, Yanling Feng, Jing Zhang, Lehao Ren, Xiaonan Zhang, Yunwen Hu, Zhenghong Yuan & Zhigang Yi
Enterovirus 71 (EV71) is a major pathogen that causes hand, foot and mouth disease (HFMD), which is a life threatening disease in certain children. The pathogenesis of EV71-caused HFMD is poorly defined due to the lack of simple and robust animal models with severe phenotypes that recapitulate symptoms observed in humans. Here, we generated the infectious clone of a clinical isolate from a severe HFMD patient. Virus rescued from the cDNA clone was infectious in...

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  • Fudan University
    607
  • Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Chinese Academy of Sciences
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