6,296 Works

m6A-immune-related lncRNA prognostic signature for predicting immune landscape and prognosis of bladder cancer

Zi-Hao Feng, Yan-Ping Liang, Jun-Jie Cen, Hao-Hua Yao, Hai-Shan Lin, Jia-Ying Li, Hui Liang, Zhu Wang, Qiong Deng, Jia-Zheng Cao, Yong Huang, Jin-Huan Wei, Jun-Hang Luo, Wei Chen & Zhen-Hua Chen
Abstract Background N6-methyladenosine (m6A) related long noncoding RNAs (lncRNAs) may have prognostic value in bladder cancer for their key role in tumorigenesis and innate immunity. Methods Bladder cancer transcriptome data and the corresponding clinical data were acquired from the Cancer Genome Atlas (TCGA) database. The m6A-immune-related lncRNAs were identified using univariate Cox regression analysis and Pearson correlation analysis. A risk model was established using least absolute shrinkage and selection operator (LASSO) Cox regression analyses, and...

Facile approach for surfactant-free synthesis of Au@ginsenoside Rh2 nanoparticles and researches on anticancer activity

Hua Yao, Xupeng Mu, Zhenhong Wei, Xiuying Li, Liya Wu, Yongri Jin, Xuwen Li, Jing Li & Jinlan Jiang
Abstract Background Inorganic nanocomposites especially Au nanostructures have exhibited outstanding physicochemical properties in biomedical fields. For further clinical applications on theranostics, especially drug delivery, numerous explorations of green and facile synthesis methods combining with pharmacoactive natural components have been investigated to construct safe and multifunctional bioactive Au nanoparticles (NPs). Ginsenoside Rh2 is protopanaxadiol type compound isolated from plants of genus Panax, with excellent anticancer effect and antioxidant activity. In this research, we prepared the novel...

Spatial and temporal evolution of paleo-highs/lows of the permian strata and its petroleum significance in the Sichuan basin, SW China

Ben Feng, Yangwen Pei, Nan Su, Zhengshuo Miao, Yu Qiu, Hua Jiang & Han Liang
The Sichuan basin has a great potential for hydrocarbon exploration. In recent years, Permian strata have been identified as one of the main target strata for natural gas exploration in the Sichuan basin. Not only the source rocks are abundant, but also there are good reservoirs due to the wide and complex continental oceanic interaction. However, there are still many shortcomings, especially the relationship between the tectonic evolution and the hydrocarbon accumulation model. Therefore, this...

Additional file 1 of LncRNA XIST from the bone marrow mesenchymal stem cell derived exosome promotes osteosarcoma growth and metastasis through miR-655/ACLY signal

Guanghui Zhu, Yu Xia, Ziyue Zhao, Aoyu Li, Hui Li & Tao Xiao
Additional file 1: Figure S1. Characterization of BMSCs. A Morphology of BMSCs under light microscope; B, C Flow cytometry and statistical analysis of the positive rates of MSC-specific surface markers (CD29 and CD90) and a hematopoietic marker CD45 (n = 3); D The analysis of ALP level after osteogenesis induction 14d(n = 3); E The level of mineralized nodules analyzed with Alizarin red staining after osteogenesis induction 21d (n = 3); F The level of...

Additional file 2 of Association between single-nucleotide polymorphism rs145497186 related to NDUFV2 and lumbar disc degeneration: a pilot case–control study

Ziyu Wang, Lu Chen, Qinghui Li, Hengshuo Zhang, Yu Shan, Linzeng Qi, Hongliang Wang & Yunzhen Chen
Additional file 2: Table S2. Hardy-Weinberg Equilibrium Test of rs28742109, rs12955018, rs987850, rs8093805, rs12965084 and rs145497186 that related to NDUFV2.

Additional file 1 of Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin–rifampicin combination against Pseudomonas aeruginosa

Mohd Hafidz Mahamad Maifiah, Yan Zhu, Brian T. Tsuji, Darren J. Creek, Tony Velkov & Jian Li
Additional file 1: Table S1. Significantly changed metabolites at 15 min. Table S2. Significantly changed metabolites at 1 h. Table S3. Significantly changed metabolites at 4 h. Table S4. Significantly changed metabolites at 24 h. Table S5. DEGs induced by polymyxin B at 1 h. Table S6. DEGs induced by rifampicin B at 1 h. Table S7. DEGs induced by the combination of polymyxin B and rifampicin at 1 h. Table S8. DEGs induced by...

Additional file 2 of Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin–rifampicin combination against Pseudomonas aeruginosa

Mohd Hafidz Mahamad Maifiah, Yan Zhu, Brian T. Tsuji, Darren J. Creek, Tony Velkov & Jian Li
Additional file 2: Figure S1. Time-kill kinetics of polymyxin B (PolyB; 1 mg/L), rifampicin (Rif; 2 mg/L), and their combination against P. aeruginosa PAO1 at starting 600 nm (OD600) of ~ 0.5 (~ 108 CFU/mL). Figure S2. GO enrichment analysis of the significantly changed genes with the polymyxin B/rifampicin combination at (A) 1 h and (B) 24 h: (i) downregulation and (ii) upregulation of various biological processes. Figure S3. Overview of metabolic pathways of P....

Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin–rifampicin combination against Pseudomonas aeruginosa

Mohd Hafidz Mahamad Maifiah, Yan Zhu, Brian T. Tsuji, Darren J. Creek, Tony Velkov & Jian Li
Abstract Background Understanding the mechanism of antimicrobial action is critical for improving antibiotic therapy. For the first time, we integrated correlative metabolomics and transcriptomics of Pseudomonas aeruginosa to elucidate the mechanism of synergistic killing of polymyxin–rifampicin combination. Methods Liquid chromatography-mass spectrometry and RNA-seq analyses were conducted to identify the significant changes in the metabolome and transcriptome of P. aeruginosa PAO1 after exposure to polymyxin B (1 mg/L) and rifampicin (2 mg/L) alone, or in combination...

Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin–rifampicin combination against Pseudomonas aeruginosa

Mohd Hafidz Mahamad Maifiah, Yan Zhu, Brian T. Tsuji, Darren J. Creek, Tony Velkov & Jian Li
Abstract Background Understanding the mechanism of antimicrobial action is critical for improving antibiotic therapy. For the first time, we integrated correlative metabolomics and transcriptomics of Pseudomonas aeruginosa to elucidate the mechanism of synergistic killing of polymyxin–rifampicin combination. Methods Liquid chromatography-mass spectrometry and RNA-seq analyses were conducted to identify the significant changes in the metabolome and transcriptome of P. aeruginosa PAO1 after exposure to polymyxin B (1 mg/L) and rifampicin (2 mg/L) alone, or in combination...

Additional file 2 of One day versus two days of hepatic arterial infusion with oxaliplatin and fluorouracil for patients with unresectable hepatocellular carcinoma

Zhicheng Lai, Yexing Huang, Dongsheng Wen, Xuanjia Lin, Anna Kan, Qijiong Li, Wei Wei, Minshan Chen, Li Xu, Minke He & Ming Shi
Additional file 2: Table S1. Treatment administration. Table S2. Univariate and multivariate analysis of overall survival and progression-free survival. Table S3. Univariate and multivariate analysis of overall survival and progression-free survival in the PSM cohort. Table S4. Intrahepatic tumor response. Table S5. Baseline characteristics and tumor response within patients performing ELISA detection.

Additional file 4 of One day versus two days of hepatic arterial infusion with oxaliplatin and fluorouracil for patients with unresectable hepatocellular carcinoma

Zhicheng Lai, Yexing Huang, Dongsheng Wen, Xuanjia Lin, Anna Kan, Qijiong Li, Wei Wei, Minshan Chen, Li Xu, Minke He & Ming Shi
Additional file 4: Figure S2. Kaplan-Meier curves of overall survival in BCLC stage C patients receiving HAIC alone (A), HAIC + TKIs (B) and HAIC + TKIs + ICIs (C) in PSM cohort. Kaplan-Meier curves of progression-free survival in BCLC stage C patients receiving HAIC alone (D), HAIC + TKIs (E) and HAIC + TKIs + ICIs (F) in PSM cohort. CI, confidence interval; HR. hazard ratio; PSM, propensity score matching.

Silencing eL31 suppresses the progression of colorectal cancer via targeting DEPDC1

Gaowa Sharen, Xiongfeng Li, Jiaxin Sun, Lei Zhang, Wen Xi, Xiaodong Zhao, Fei Han, Longlong Jia, Rong A, Haidong Cheng & Mingxing Hou
Abstract Background Colorectal cancer (CRC) is one of the most commonly diagnosed human malignancies. Ribosomal protein L31 (RPL31, aka eL31) is a component of the 60S large ribosomal subunit, and its expression pattern and functional role in CRC have not been reported. Methods Herein, we identified that eL31 protein level was dramatically increased in CRC tissues through using IHC analysis. More notably, elevated eL31 was associated with larger tumor size and shorter overall survival. Besides,...

Additional file 1 of Silencing eL31 suppresses the progression of colorectal cancer via targeting DEPDC1

Gaowa Sharen, Xiongfeng Li, Jiaxin Sun, Lei Zhang, Wen Xi, Xiaodong Zhao, Fei Han, Longlong Jia, Rong A, Haidong Cheng & Mingxing Hou
Additional file 1: Figure S1. (A) The transfection efficiencies of sheL31-1 and sheL31-2 in HCT 116 cells were detected by qRT-PCR. (B) The transfection efficiencies of sheL31-1 and sheL31-2 in HCT 116 and RKO cells were evaluated through observing the fluorescence inside cells. Magnification times: 200×. (C) The densitometric analyses of Akt, P-Akt, CCND1, CDK6 and PIK3CA proteins expression. * P < 0.05, *** P < 0.001. Figure S2. (A) The volcano plot of gene...

Identification of the origin of tumor in vein: comparison between CEUS LI-RADS v2017 and v2016 for patients at high risk

Wen-juan Tong, Mei-qing Cheng, Man-xia Lin, Hang-tong Hu, Jia-min Pan, Hui Huang, Ying Wang, Xiao-yan Xie, Ming-de Lu, Ming Kuang, Yang Huang & Wei Wang
Abstract Objectives To compare the diagnostic performance of the Contrast-Enhanced Ultrasound (CEUS) Liver Imaging Report and Data System (LI-RADS) v2016 and v2017 in identifying the origin of tumor in vein (TIV). Methods From April 2014 to December 2018, focal liver lesions (FLLs) accompanied by TIV formation in patients at high risk for hepatocellular carcinoma (HCC) were enrolled. Histologic evaluation or composite imaging reference standard were served as the reference standard. Each case was categorized according...

Additional file 1 of Mechanistic insights into the health benefits of fish-oil supplementation against fine particulate matter air pollution: a randomized controlled trial

Lu Zhou, Yixuan Jiang, Zhijing Lin, Renjie Chen, Yue Niu & Haidong Kan
Supplementary Material 1

Additional file 1 of Mechanistic insights into the health benefits of fish-oil supplementation against fine particulate matter air pollution: a randomized controlled trial

Lu Zhou, Yixuan Jiang, Zhijing Lin, Renjie Chen, Yue Niu & Haidong Kan
Supplementary Material 1

Additional file 1 of Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei

Shanshan Jiang, Yue Wang, Qin Liu, Qinqin Zhao, Liwei Gao, Xin Song, Xuezhi Li, Yinbo Qu & Guodong Liu
Additional file 1: Table S1. Primers used for strain construction and verification in this study. Table S2. Primers used for qPCR in this study. Table S3. Copy numbers of poman5A gene in the constructed strains measured by qPCR. Figure S1. Map of plasmid pM5p.

Additional file 1 of Coexistence of chronic hyperalgesia and multilevel neuroinflammatory responses after experimental SCI: a systematic approach to profiling neuropathic pain

Lei Wang, Mehmet A. Gunduz, Ana T. Semeano, Enis C. Yılmaz, Feras A. H. Alanazi, Ozan B. Imir, Ulas Yener, Christian A. Arbelaez, Esteban Usuga & Yang D. Teng
Additional file 1: Table S1. Scores of spontaneous pain-related physical parameters.

Additional file 2 of Coexistence of chronic hyperalgesia and multilevel neuroinflammatory responses after experimental SCI: a systematic approach to profiling neuropathic pain

Lei Wang, Mehmet A. Gunduz, Ana T. Semeano, Enis C. Yılmaz, Feras A. H. Alanazi, Ozan B. Imir, Ulas Yener, Christian A. Arbelaez, Esteban Usuga & Yang D. Teng
Additional file 2: Table S2. Immunohistochemical reaction protocols.

Coexistence of chronic hyperalgesia and multilevel neuroinflammatory responses after experimental SCI: a systematic approach to profiling neuropathic pain

Lei Wang, Mehmet A. Gunduz, Ana T. Semeano, Enis C. Yılmaz, Feras A. H. Alanazi, Ozan B. Imir, Ulas Yener, Christian A. Arbelaez, Esteban Usuga & Yang D. Teng
Abstract Background People with spinal cord injury (SCI) frequently develop neuropathic pain (NP) that worsens disability and diminishes rehabilitation efficacy. Chronic NP is presently incurable due to poor understanding of underlying mechanisms. We hypothesized that multilocus neuroinflammation (NIF) might be a driver of SCI NP, and tested it by investigating whether NP coexisted with central NIF, neurotransmission (NTM), neuromodulation (NML) and neuroplasticity (NPL) changes post-SCI. Methods Female Sprague–Dawley rats (230–250 g) with T10 compression or...

Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei

Shanshan Jiang, Yue Wang, Qin Liu, Qinqin Zhao, Liwei Gao, Xin Song, Xuezhi Li, Yinbo Qu & Guodong Liu
Abstract The fungus Trichoderma reesei is a powerful host for secreted production of proteins. The promoter of cdna1 gene, which encodes a small basic protein of unknown function and high expression, is commonly used for constitutive protein production in T. reesei. Nevertheless, the production level of proteins driven by this promoter still needs to be improved. Here, we identified that the region 600- to 700-bp upstream of the start codon is critical for the efficiency...

Cloning of goat PGAM2 gene and its overexpression promotes the differentiation of intramuscular preadipocytes

Xiao Ma, Yuxue Wang, Xinyi Li, Jian Wang, Botao Wang, Yaqiu Lin & Yan Xiong
As a member of the PGAMs family, PGAM2 has been proved to catalyze the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. However, it is unclear whether PGAM2 has a role in regulating differentiation in goat intramuscular preadipocytes. Here, this study was carried to clone the open reading frame (ORF) of goat PGAM2, elucidate its molecular and expressional characteristics, and evaluate the involvement in adipogenesis of intramuscular preadipocytes. According to our...

Cloning of goat PGAM2 gene and its overexpression promotes the differentiation of intramuscular preadipocytes

Xiao Ma, Yuxue Wang, Xinyi Li, Jian Wang, Botao Wang, Yaqiu Lin & Yan Xiong
As a member of the PGAMs family, PGAM2 has been proved to catalyze the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. However, it is unclear whether PGAM2 has a role in regulating differentiation in goat intramuscular preadipocytes. Here, this study was carried to clone the open reading frame (ORF) of goat PGAM2, elucidate its molecular and expressional characteristics, and evaluate the involvement in adipogenesis of intramuscular preadipocytes. According to our...

Compact structured illumination microscopy with high spatial frequency diffractive lattice patterns

cilong zhang, Ning Xu & Qiaofeng Tan
Structured illumination microscopy (SIM) enables live-cell super-resolution imaging with wide field of view (FOV) and high imaging speed, but the illumination system is usually bulky. With the advantages of small structure and high efficiency, lattice patterns assisted by diffractive optical elements (DOEs) have been used for structured illumination in SIM. But it is still challenging to raise the spatial frequency of diffractive lattice patterns when using traditional DOE design method, and thus the super-resolution imaging...

Supplementary document for Compact structured illumination microscopy with high spatial frequency diffractive lattice patterns - 6069450.pdf

cilong zhang, Ning Xu & Qiaofeng Tan
supplemental document

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